The Experts below are selected from a list of 141 Experts worldwide ranked by ideXlab platform
Ofelia Acosta - One of the best experts on this subject based on the ideXlab platform.
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Experimental Intoxication of guinea pigs with ipomoea carnea behavioural and neuropathological alterations
Toxicon, 2013Co-Authors: Luciana A Cholich, Mercedes Marquez, Marti Pumarola I Batlle, E J Gimeno, Gladys P Teibler, Elvio E Rios, Ofelia AcostaAbstract:Ipomoea carnea is a toxic plant that affects goats, with symptoms being characterised by nervous disorders and death. Swainsonine and calystegines are the principal toxic components isolated from I. carnea, which also yields lysergic acid derivatives. The aim of this study was to improve the clinical characterisation of Experimental Intoxication by I. carnea in guinea pigs through the evaluation of behavioural changes and to perform a thorough histopathological analysis of the affected CNS. Leaves of I. carnea were administered to guinea pigs. Open-field gait analysis and monoamine levels were measured. The poisoned animals exhibited increased vocalisation, lethargy, and a reduction in the locomotion frequency after the fourth week of Intoxication, as demonstrated in the open-field test. Significant differences were observed in hind-limb gait width by the last week of Intoxication. After 65 days, the guinea pigs were euthanised, necropsied, and examined using light and electron microscopy. At the end of the experiment, plasma serotonin decreased. In contrast, dopamine decreased, and noradrenaline increased in urine. Brain sections were evaluated with conventional histological methods and immunohistochemistry (IHC), as well as by transmission electron microscopy (TEM). Vacuoles were observed throughout the brain, but they were particularly prominent in the brainstem. In addition, there were PAS-negative regions, and the Nissl substance was dispersed or absent, which was confirmed with the Kluver-Barreda stain. Moderate microgliosis was observed by immunohistochemistry. In the medulla oblongata, numerous ubiquitin-positive spheroids together with neuronal degeneration were observed in the nucleus gracilis/cuneatus. Furthermore, vacuoles were observed in astrocytes, oligodendrocytes, and endothelial cells by TEM. Our results showed that the behavioural effects may have been caused by alterations in the brain in conjunction with changes in monoamine levels. This research confirms the utility of this model for studying the pathogenesis of plant-induced lysosomal storage diseases.
Paulo Henrique Jorge Da Cunha - One of the best experts on this subject based on the ideXlab platform.
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evaluation of the resistance of nellore curraleiro pe duro and pantaneiro cattle breeds by Experimental Intoxication of palicourea marcgravii
Toxicon, 2019Co-Authors: Juliana J Serodio, Luma Tatiana Silva Castro, Tabata Laiza Morais, Roberta Dias Da Silva Cunha, Fabiano Jose Ferreira De Santana, R S Juliano, Jose Renato Junqueira Borges, Maria Clorinda Soares Fioravanti, Paulo Henrique Jorge Da CunhaAbstract:Abstract Palicourea marcgravii is the most important toxic plant in Brazil due to its acute toxicity (sudden death), palatability, cumulative effect and broad geographical distribution. The study aimed to evaluate the resistance between cattle of different breeds by Experimental Intoxication of Palicourea marcgravii. Eighteen males were randomly divided into three groups (Nellore, Curraleiro Pe-Duro and Pantaneiro), six animals in each group. All animals were Experimentally intoxicated with P. marcgravii at 0.5 mg/kg of equivalent sodium monofluoracetate acid, receiving one single oral dose. All cattle presented symptoms and the toxic plant caused death in all cattle of the Nellore and Pantaneiro breeds and only three Curraleiro cattle. No changes were observed in the leukogram, fibrinogen, albumin, total protein and bilirubins. Hyperglycemia and increased of aspartate transaminase, creatinine kinase, urea and creatinine were detected in all breeds. Changes in the values of the gamma glutamyl transferase were individualized and did not follow a standard response. Fourteen cattle presented the histopathological findings of renal hydropic degeneration. The lower death rate of Curraleiro cattle indicates a higher resistance than cattle of the Nellore and Pantaneiro breeds.
Luciana A Cholich - One of the best experts on this subject based on the ideXlab platform.
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Experimental Intoxication of guinea pigs with ipomoea carnea behavioural and neuropathological alterations
Toxicon, 2013Co-Authors: Luciana A Cholich, Mercedes Marquez, Marti Pumarola I Batlle, E J Gimeno, Gladys P Teibler, Elvio E Rios, Ofelia AcostaAbstract:Ipomoea carnea is a toxic plant that affects goats, with symptoms being characterised by nervous disorders and death. Swainsonine and calystegines are the principal toxic components isolated from I. carnea, which also yields lysergic acid derivatives. The aim of this study was to improve the clinical characterisation of Experimental Intoxication by I. carnea in guinea pigs through the evaluation of behavioural changes and to perform a thorough histopathological analysis of the affected CNS. Leaves of I. carnea were administered to guinea pigs. Open-field gait analysis and monoamine levels were measured. The poisoned animals exhibited increased vocalisation, lethargy, and a reduction in the locomotion frequency after the fourth week of Intoxication, as demonstrated in the open-field test. Significant differences were observed in hind-limb gait width by the last week of Intoxication. After 65 days, the guinea pigs were euthanised, necropsied, and examined using light and electron microscopy. At the end of the experiment, plasma serotonin decreased. In contrast, dopamine decreased, and noradrenaline increased in urine. Brain sections were evaluated with conventional histological methods and immunohistochemistry (IHC), as well as by transmission electron microscopy (TEM). Vacuoles were observed throughout the brain, but they were particularly prominent in the brainstem. In addition, there were PAS-negative regions, and the Nissl substance was dispersed or absent, which was confirmed with the Kluver-Barreda stain. Moderate microgliosis was observed by immunohistochemistry. In the medulla oblongata, numerous ubiquitin-positive spheroids together with neuronal degeneration were observed in the nucleus gracilis/cuneatus. Furthermore, vacuoles were observed in astrocytes, oligodendrocytes, and endothelial cells by TEM. Our results showed that the behavioural effects may have been caused by alterations in the brain in conjunction with changes in monoamine levels. This research confirms the utility of this model for studying the pathogenesis of plant-induced lysosomal storage diseases.
E. Galecka - One of the best experts on this subject based on the ideXlab platform.
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Effects of CGS 9896 in acute Experimental Intoxication with fluostigmine
Archives of Toxicology, 1990Co-Authors: S. Rump, W. Raszewski, T. Gidynska, E. GaleckaAbstract:The therapeutic effectiveness of CGS 9896, a novel potent anxiolytic and anticonvulsant, in organophosphate Intoxication was studied. It was demonstrated that this drug, used as an adjunct to a mixture of atropine and obidoxime, led to a rise in the LD50 of fluostigmine in mice of more than 150 times and more than 138 times for 2 and 24 h, respectively. After 24 h, this effect was twice that of diazepam, which served as a reference drug.
Marti Pumarola I Batlle - One of the best experts on this subject based on the ideXlab platform.
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Experimental Intoxication of guinea pigs with ipomoea carnea behavioural and neuropathological alterations
Toxicon, 2013Co-Authors: Luciana A Cholich, Mercedes Marquez, Marti Pumarola I Batlle, E J Gimeno, Gladys P Teibler, Elvio E Rios, Ofelia AcostaAbstract:Ipomoea carnea is a toxic plant that affects goats, with symptoms being characterised by nervous disorders and death. Swainsonine and calystegines are the principal toxic components isolated from I. carnea, which also yields lysergic acid derivatives. The aim of this study was to improve the clinical characterisation of Experimental Intoxication by I. carnea in guinea pigs through the evaluation of behavioural changes and to perform a thorough histopathological analysis of the affected CNS. Leaves of I. carnea were administered to guinea pigs. Open-field gait analysis and monoamine levels were measured. The poisoned animals exhibited increased vocalisation, lethargy, and a reduction in the locomotion frequency after the fourth week of Intoxication, as demonstrated in the open-field test. Significant differences were observed in hind-limb gait width by the last week of Intoxication. After 65 days, the guinea pigs were euthanised, necropsied, and examined using light and electron microscopy. At the end of the experiment, plasma serotonin decreased. In contrast, dopamine decreased, and noradrenaline increased in urine. Brain sections were evaluated with conventional histological methods and immunohistochemistry (IHC), as well as by transmission electron microscopy (TEM). Vacuoles were observed throughout the brain, but they were particularly prominent in the brainstem. In addition, there were PAS-negative regions, and the Nissl substance was dispersed or absent, which was confirmed with the Kluver-Barreda stain. Moderate microgliosis was observed by immunohistochemistry. In the medulla oblongata, numerous ubiquitin-positive spheroids together with neuronal degeneration were observed in the nucleus gracilis/cuneatus. Furthermore, vacuoles were observed in astrocytes, oligodendrocytes, and endothelial cells by TEM. Our results showed that the behavioural effects may have been caused by alterations in the brain in conjunction with changes in monoamine levels. This research confirms the utility of this model for studying the pathogenesis of plant-induced lysosomal storage diseases.
