Exposed Collagen

14,000,000 Leading Edge Experts on the ideXlab platform

Scan Science and Technology

Contact Leading Edge Experts & Companies

Scan Science and Technology

Contact Leading Edge Experts & Companies

The Experts below are selected from a list of 13638 Experts worldwide ranked by ideXlab platform

Junji Tagami - One of the best experts on this subject based on the ideXlab platform.

  • elemental distributions and microtensile bond strength of the adhesive interface to normal and caries affected dentin
    Journal of Biomedical Materials Research Part B, 2005
    Co-Authors: Masatoshi Nakajima, Yuichi Kitasako, Mamiko Okuda, Richard M Foxton, Junji Tagami
    Abstract:

    The aim of this study was to evaluate the microtensile bond strength (μTBS) and the elemental contents of the adhesive interface created to normal versus caries-affected dentin. Extracted human molars with coronal carious lesions were used in this study. A self-etching primer/adhesive system (Clearfil Protect Bond) was applied to flat dentin surfaces with normal and caries-affected dentin according to the manufacturer's instructions. After 24 h water storage, the bonded specimens were cross-sectioned and subjected to a μTBS test and electron probe microanalysis for the elemental distributions [calcium (Ca), phosphorus (P), magnesium (Mg), and nitrogen (N)] of the resin–dentin interface after gold sputter-coating. The μTBS to caries-affected dentin was lower than that of normal dentin. The demineralized zone of the caries-affected dentin–resin interface was thicker than that of normal dentin (approximately 3 μm thick in normal dentin; 8 μm thick in caries-affected dentin), and Ca and P in both types of dentin gradually increased from the interface to the underlying dentin. The caries-affected dentin had lost most of its Mg content. The distributions of the minerals, Ca, P, and Mg, at the adhesive interface to caries-affected dentin were different from normal dentin. Moreover, a N peak, which was considered to be the Collagen-rich zone resulting from incomplete resin infiltration of Exposed Collagen, was observed to be thicker within the demineralized zone of caries-affected dentin compared with normal dentin. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 72B: 268–275, 2005

  • elemental distributions and microtensile bond strength of the adhesive interface to normal and caries affected dentin
    Journal of Biomedical Materials Research, 2005
    Co-Authors: Masatoshi Nakajima, Yuichi Kitasako, Mamiko Okuda, Richard M Foxton, Junji Tagami
    Abstract:

    The aim of this study was to evaluate the microtensile bond strength (μTBS) and the elemental contents of the adhesive interface created to normal versus caries-affected dentin. Extracted human molars with coronal cariouslesions were used in this study. A self-etching primer/adhesive system (Clearfil Protect Bond) was applied to flat dentin surfaces with normal and caries-affected dentin according to the manufacturer's instructions. After 24 h water storage, the bonded specimens were cross-sectioned and subjected to a μTBS test and electron probe microanalysis for the elemental distributions [calcium (Ca), phosphorus (P), magnesium (Mg), and nitrogen (N)] of the resin-dentin interface after gold sputter-coating. The μTBS to caries-affected dentin was lower than that of normal dentin. The demineralized zone of the caries-affected dentin-resin interface was thicker than that of normal dentin (approximately 3 μm thick in normal dentin; 8 μm thick in caries-affected dentin), and Ca and P in both types of dentin gradually increased from the interface to the underlying dentin. The caries-affected dentin had lost most of its Mg content. The distributions of the minerals, Ca, P, and Mg, at the adhesive interface to caries-affected dentin were different from normal dentin. Moreover, a N peak, which was considered to be the Collagen-rich zone resulting from incomplete resin infiltration of Exposed Collagen, was observed to be thicker within the demineralized zone of caries-affected dentin compared with normal dentin.

Jayalakshmi Vaidyanathan - One of the best experts on this subject based on the ideXlab platform.

  • recent advances in the theory and mechanism of adhesive resin bonding to dentin a critical review
    Journal of Biomedical Materials Research Part B, 2009
    Co-Authors: T. K. Vaidyanathan, Jayalakshmi Vaidyanathan
    Abstract:

    Dentin bonding issues involving adhesive resins have attracted considerable research interest in recent years. An important advance due to the ongoing research is the concept of hybridization of the tissue with primer/adhesive systems. Hybridization involves permeation of primer monomer into the tissue substrate. Although the mechanism of adhesive permeation and interaction with tissue may be complex, significant advances have been made. In systems where etching precedes priming and bonding steps, the Hoy's solubility parameter compatibility of the primer formulation with that of demineralized dentin matrix may determine adhesive permeability. Monomer permeation brings the primer atoms in closer contact with the substrate atoms, leading to adhesive interactions through van der Waals, hydrogen bonding, and electrostatic interactions. In self-etch primer systems, stronger electrostatic interaction between primer monomers and hydroxyapatite has been used to explain the adhesion process. These interactions have been computer-modeled and analyzed. Such interactions and subsequent polymerization of the monomer promote improved bond strength and efficient margin sealing. Incomplete permeation of monomer into the full depth of demineralized region may, however, leave Exposed Collagen fibrils and cause nanoleakage of water into these regions through a 20–100 nm sized marginal gap, leading to subsequent hydrolytic degradation of these Collagen fibrils and the hybrid layer. Microleakage is also a problem in some single step formulations. In this review, we analyze these current theoretical and mechanism-related issues of interest in adhesive resin bonding to dentin, and outline the continuing problems that need to be overcome in the future. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009

  • Recent advances in the theory and mechanism of adhesive resin bonding to dentin: A critical review
    Journal of Biomedical Materials Research - Part B Applied Biomaterials, 2009
    Co-Authors: T. K. Vaidyanathan, Jayalakshmi Vaidyanathan
    Abstract:

    Dentin bonding issues involving adhesive resins have attracted considerable research interest in recent years. An important advance due to the ongoing research is the concept of hybridization of the tissue with primer/adhesive systems. Hybridization involves permeation of primer monomer into the tissue substrate. Although the mechanism of adhesive permeation and interaction with tissue may be complex, significant advances have been made. In systems where etching precedes priming and bonding steps, the Hoy's solubility parameter compatibility of the primer formulation with that of demineralized dentin matrix may determine adhesive permeability. Monomer permeation brings the primer atoms in closer contact with the substrate atoms, leading to adhesive interactions through van der Waals, hydrogen bonding, and electrostatic interactions. In self-etch primer systems, stronger electrostatic interaction between primer monomers and hydroxyapatite has been used to explain the adhesion process. These interactions have been computer-modeled and analyzed. Such interactions and subsequent polymerization of the monomer promote improved bond strength and efficient margin sealing. Incomplete permeation of monomer into the full depth of demineralized region may, however, leave Exposed Collagen fibrils and cause nanoleakage of water into these regions through a 20-100 nm sized marginal gap, leading to subsequent hydrolytic degradation of these Collagen fibrils and the hybrid layer. Microleakage is also a problem in some single step formulations. In this review, we analyze these current theoretical and mechanism-related issues of interest in adhesive resin bonding to dentin, and outline the continuing problems that need to be overcome in the future.

Masatoshi Nakajima - One of the best experts on this subject based on the ideXlab platform.

  • elemental distributions and microtensile bond strength of the adhesive interface to normal and caries affected dentin
    Journal of Biomedical Materials Research Part B, 2005
    Co-Authors: Masatoshi Nakajima, Yuichi Kitasako, Mamiko Okuda, Richard M Foxton, Junji Tagami
    Abstract:

    The aim of this study was to evaluate the microtensile bond strength (μTBS) and the elemental contents of the adhesive interface created to normal versus caries-affected dentin. Extracted human molars with coronal carious lesions were used in this study. A self-etching primer/adhesive system (Clearfil Protect Bond) was applied to flat dentin surfaces with normal and caries-affected dentin according to the manufacturer's instructions. After 24 h water storage, the bonded specimens were cross-sectioned and subjected to a μTBS test and electron probe microanalysis for the elemental distributions [calcium (Ca), phosphorus (P), magnesium (Mg), and nitrogen (N)] of the resin–dentin interface after gold sputter-coating. The μTBS to caries-affected dentin was lower than that of normal dentin. The demineralized zone of the caries-affected dentin–resin interface was thicker than that of normal dentin (approximately 3 μm thick in normal dentin; 8 μm thick in caries-affected dentin), and Ca and P in both types of dentin gradually increased from the interface to the underlying dentin. The caries-affected dentin had lost most of its Mg content. The distributions of the minerals, Ca, P, and Mg, at the adhesive interface to caries-affected dentin were different from normal dentin. Moreover, a N peak, which was considered to be the Collagen-rich zone resulting from incomplete resin infiltration of Exposed Collagen, was observed to be thicker within the demineralized zone of caries-affected dentin compared with normal dentin. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 72B: 268–275, 2005

  • elemental distributions and microtensile bond strength of the adhesive interface to normal and caries affected dentin
    Journal of Biomedical Materials Research, 2005
    Co-Authors: Masatoshi Nakajima, Yuichi Kitasako, Mamiko Okuda, Richard M Foxton, Junji Tagami
    Abstract:

    The aim of this study was to evaluate the microtensile bond strength (μTBS) and the elemental contents of the adhesive interface created to normal versus caries-affected dentin. Extracted human molars with coronal cariouslesions were used in this study. A self-etching primer/adhesive system (Clearfil Protect Bond) was applied to flat dentin surfaces with normal and caries-affected dentin according to the manufacturer's instructions. After 24 h water storage, the bonded specimens were cross-sectioned and subjected to a μTBS test and electron probe microanalysis for the elemental distributions [calcium (Ca), phosphorus (P), magnesium (Mg), and nitrogen (N)] of the resin-dentin interface after gold sputter-coating. The μTBS to caries-affected dentin was lower than that of normal dentin. The demineralized zone of the caries-affected dentin-resin interface was thicker than that of normal dentin (approximately 3 μm thick in normal dentin; 8 μm thick in caries-affected dentin), and Ca and P in both types of dentin gradually increased from the interface to the underlying dentin. The caries-affected dentin had lost most of its Mg content. The distributions of the minerals, Ca, P, and Mg, at the adhesive interface to caries-affected dentin were different from normal dentin. Moreover, a N peak, which was considered to be the Collagen-rich zone resulting from incomplete resin infiltration of Exposed Collagen, was observed to be thicker within the demineralized zone of caries-affected dentin compared with normal dentin.

Luis Roberto Marcondes Martins - One of the best experts on this subject based on the ideXlab platform.

  • effect of dimethyl sulfoxide wet bonding technique on hybrid layer quality and dentin bond strength
    Dental Materials, 2015
    Co-Authors: Thiago Henrique Scarabello Stape, Leo Tjaderhane, Marcelo Rocha Marques, Flavio Henrique Baggio Aguiar, Luis Roberto Marcondes Martins
    Abstract:

    Abstract Objectives This study examined the effect of a dimethyl sulfoxide (DMSO) wet bonding technique on the resin infiltration depths at the bonded interface and dentin bond strength of different adhesive systems. Methods Flat dentin surfaces of 48 human third molars were treated with 50% DMSO (experimental groups) or with distilled water (controls) before bonding using an etch-and-rinse (SBMP: Scotchbond Multi-Purpose, 3M ESPE) or a self-etch (Clearfil: Clearfil SE Bond, Kuraray) adhesive system. The restored crown segments (n = 12/group) were stored in distilled water (24 h) and sectioned for interfacial analysis of Exposed Collagen using Masson's Trichrome staining and for microtensile bond strength testing. The extent of Exposed Collagen was measured using light microscopy and a histometric analysis software. Failure modes were examined by SEM. Data was analyzed by two-way ANOVA followed by Tukey Test (α = 0.05). Results The interaction of bonding protocol and adhesive system had significant effects on the extension of Exposed Collagen matrix (p   0.05). Significance DMSO-wet bonding was effective to improve the quality of resin–dentin bonds of the tested etch-and-rinse adhesives by reducing the extent of Exposed Collagen matrix at the base of the resin–dentin biopolymer. The improved penetration of adhesive monomers is reflected as an increase in the immediate bond strength when the DMSO-wet bonding technique is used with a water-based etch-and-rinse adhesive.

Leo Tjaderhane - One of the best experts on this subject based on the ideXlab platform.

  • effect of dimethyl sulfoxide wet bonding technique on hybrid layer quality and dentin bond strength
    Dental Materials, 2015
    Co-Authors: Thiago Henrique Scarabello Stape, Leo Tjaderhane, Marcelo Rocha Marques, Flavio Henrique Baggio Aguiar, Luis Roberto Marcondes Martins
    Abstract:

    Abstract Objectives This study examined the effect of a dimethyl sulfoxide (DMSO) wet bonding technique on the resin infiltration depths at the bonded interface and dentin bond strength of different adhesive systems. Methods Flat dentin surfaces of 48 human third molars were treated with 50% DMSO (experimental groups) or with distilled water (controls) before bonding using an etch-and-rinse (SBMP: Scotchbond Multi-Purpose, 3M ESPE) or a self-etch (Clearfil: Clearfil SE Bond, Kuraray) adhesive system. The restored crown segments (n = 12/group) were stored in distilled water (24 h) and sectioned for interfacial analysis of Exposed Collagen using Masson's Trichrome staining and for microtensile bond strength testing. The extent of Exposed Collagen was measured using light microscopy and a histometric analysis software. Failure modes were examined by SEM. Data was analyzed by two-way ANOVA followed by Tukey Test (α = 0.05). Results The interaction of bonding protocol and adhesive system had significant effects on the extension of Exposed Collagen matrix (p   0.05). Significance DMSO-wet bonding was effective to improve the quality of resin–dentin bonds of the tested etch-and-rinse adhesives by reducing the extent of Exposed Collagen matrix at the base of the resin–dentin biopolymer. The improved penetration of adhesive monomers is reflected as an increase in the immediate bond strength when the DMSO-wet bonding technique is used with a water-based etch-and-rinse adhesive.