The Experts below are selected from a list of 21 Experts worldwide ranked by ideXlab platform
Friedhelm Meinhardt - One of the best experts on this subject based on the ideXlab platform.
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Extrachromosomal Inheritance: Yeast Linear Killer Plasmids as a Tool in Genetic Engineering
Progress in Botany, 1995Co-Authors: Jürgen Schründer, Friedhelm MeinhardtAbstract:In principle, every stably replicated DNA species can serve as a vector for cloning foreign DNA in yeast or any other organism, in which such DNA is found. In strains of Saccharomyces cerevisiae a high copy number, autonomously replicating, Extrachromosomal DNA element, the 2 µm DNA, was found to exist (Sinclair et al. 1967). This covalently closed circular DNA element resembles in many respects bacterial plasmids. Since it is present in approximately 50 copies per cell it is relatively stably maintained. It was used to construct vectors for cloning and expression of foreign genes in Saccharomyces cerevisiae (for review see Hollenberg 1982). The occurrence of circular plasmids is not restricted to baker’s yeast, because similar elements were also detected in Zygosaccharomyces (Tohe and Utatsu 1985) and in Kluyveromyces drosophilarum (Chen et al. 1986). The latter plasmid, pKD1, is 1.6 µm in size and was also transferred and stably maintained in other yeast species of the genus, i.e., Kluyveromyces lactis (Bianchi et al. 1987).
Curt Carlbom - One of the best experts on this subject based on the ideXlab platform.
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Reciprocal Extrachromosomal Inheritance in rye (Secale cereale L.)
Hereditas, 2009Co-Authors: Sune Fröst, Ludvigs Vaivars, Curt CarlbomAbstract:In an inbred line of Steel rye (Stalrag), a chlorophyll mutant (status paralbomaculatus) arose spontaneously that displayed both Extrachromosomal maternal and paternal Inheritance in crosses. Striata plants gave rise to albino, striata, and normal offspring when selfed. When the striata mutant was used as the maternal or paternal parent and crossed with normal chlorophyllous plants, the progenies were comprised of striata and normal individuals. Upon selfing, the F1 and F2striata plants gave rise to variable non-Mendelian ratios of albina, striata, and normal offspring–the ratios being proportionate to the amount of normal plastid-bearing to defective plastid-bearing tissue in the germ lines (anthers and ovaries). The striata mutant of Steel rye is believed to be the first confirmed case of a status paralbomaculatus mutant in the Gramineae. Thin-layer chromatographic studies confirmed that flavanoid and phenolic compounds are prevalent in the plastids in Steel rye. Seven of the 10 spots in the composite chromatograms of 19 F3 plants representing the three plastom phenotypes displayed differences.
Edmund S. Idziak - One of the best experts on this subject based on the ideXlab platform.
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Plasmids in Listeria monocytogenes and other Listeria species
Canadian journal of microbiology, 1992Co-Authors: P.i. Peterkin, Mary-ann Gardiner, Naeem Malik, Edmund S. IdziakAbstract:One hundred and twenty-two food, clinical, and veterinary strains of Listeria monocytogenes were examined for the presence of plasmids. Twenty-five (20%) contained plasmids, which varied from 1.3 to 66 MDa in size. Of 10 strainsof other Listeria species (L. innocua, L. ivanovii, L. welshimeri, L. seeligeri, L. grayi, and L. murrayi) examined, seven (70%) contained plasmids, varying from 38 to 53 MDa. No strains with multiple plasmids were found. Plasmids of identical size were isolated from related strains in some, although not all, cases. The presence of a plasmid in a strain was not related to phenotypic characters of known Extrachromosomal Inheritance. Key words: plasmid, Listeria spp., Listeria monocytogenes.
Jürgen Schründer - One of the best experts on this subject based on the ideXlab platform.
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Extrachromosomal Inheritance: Yeast Linear Killer Plasmids as a Tool in Genetic Engineering
Progress in Botany, 1995Co-Authors: Jürgen Schründer, Friedhelm MeinhardtAbstract:In principle, every stably replicated DNA species can serve as a vector for cloning foreign DNA in yeast or any other organism, in which such DNA is found. In strains of Saccharomyces cerevisiae a high copy number, autonomously replicating, Extrachromosomal DNA element, the 2 µm DNA, was found to exist (Sinclair et al. 1967). This covalently closed circular DNA element resembles in many respects bacterial plasmids. Since it is present in approximately 50 copies per cell it is relatively stably maintained. It was used to construct vectors for cloning and expression of foreign genes in Saccharomyces cerevisiae (for review see Hollenberg 1982). The occurrence of circular plasmids is not restricted to baker’s yeast, because similar elements were also detected in Zygosaccharomyces (Tohe and Utatsu 1985) and in Kluyveromyces drosophilarum (Chen et al. 1986). The latter plasmid, pKD1, is 1.6 µm in size and was also transferred and stably maintained in other yeast species of the genus, i.e., Kluyveromyces lactis (Bianchi et al. 1987).
Sune Fröst - One of the best experts on this subject based on the ideXlab platform.
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Reciprocal Extrachromosomal Inheritance in rye (Secale cereale L.)
Hereditas, 2009Co-Authors: Sune Fröst, Ludvigs Vaivars, Curt CarlbomAbstract:In an inbred line of Steel rye (Stalrag), a chlorophyll mutant (status paralbomaculatus) arose spontaneously that displayed both Extrachromosomal maternal and paternal Inheritance in crosses. Striata plants gave rise to albino, striata, and normal offspring when selfed. When the striata mutant was used as the maternal or paternal parent and crossed with normal chlorophyllous plants, the progenies were comprised of striata and normal individuals. Upon selfing, the F1 and F2striata plants gave rise to variable non-Mendelian ratios of albina, striata, and normal offspring–the ratios being proportionate to the amount of normal plastid-bearing to defective plastid-bearing tissue in the germ lines (anthers and ovaries). The striata mutant of Steel rye is believed to be the first confirmed case of a status paralbomaculatus mutant in the Gramineae. Thin-layer chromatographic studies confirmed that flavanoid and phenolic compounds are prevalent in the plastids in Steel rye. Seven of the 10 spots in the composite chromatograms of 19 F3 plants representing the three plastom phenotypes displayed differences.
