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Boosya Bunnag - One of the best experts on this subject based on the ideXlab platform.
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Effect of Extraction Temperature on the diffusion coefficient of polysaccharides from Spirulina and the optimal separation method
Biotechnology and Bioprocess Engineering, 2014Co-Authors: Ratana Chaiklahan, Panya Triratana, Nattayaporn Chirasuwan, Boosya BunnagAbstract:The Extraction Temperature had a significant impact on the concentration of polysaccharides derived from solid-liquid Extraction of Spirulina. The polysaccharide concentration was significantly higher when the Extraction was performed at 90°C than when it was performed at 80, 70, and 50°C. This result is related to the diffusion coefficients of the polysaccharides, which increased from 1.07 × 10−12 at 50°C to 3.02 × 10−12 m2/sec at 90°C. Using the Arrhenius equation, the pre-exponential factor (D0) and the activation energy (Ea) for Spirulina polysaccharide Extraction were calculated as 7.958 × 10−9 m2/sec and 24.0 kJ/mol, respectively. Among the methods used for the separation of Spirulina polysaccharides, cetyltrimethylammonium bromide (CTAB, method I) and organic solvent (ethanol, in methods II and III) provided similar yields of polysaccharides. However, the separation of polysaccharides using an ultrafiltration (UF) process (method III) and ethanol precipitation was superior to separation via CTAB or vacuum rotary evaporation (method II). The use of a membrane with a molecular weight cut-off (MWCO) of 30 kDa and an area of 0.01 m2 at a feed pressure of 103 kPa with a mean permeate flux of 39.3 L/m2/h and a retention rate of 95% was optimal for the UF process. The addition of two volumes (v/v) of ethanol, which gave a total polysaccharide content of approximately 4% dry weight, was found to be most suitable for polysaccharide precipitation. The results of a Sepharose 6B column separation showed that the molecular weights of the polysaccharides in fractions I and II were 212 and 12.6 kDa, respectively.
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Effect of Extraction Temperature on the diffusion coefficient of polysaccharides from Spirulina and the optimal separation method
Biotechnology and Bioprocess Engineering, 2014Co-Authors: Ratana Chaiklahan, Panya Triratana, Nattayaporn Chirasuwan, Suvit Tia, Boosya BunnagAbstract:The Extraction Temperature had a significant impact on the concentration of polysaccharides derived from solid-liquid Extraction of Spirulina. The polysaccharide concentration was significantly higher when the Extraction was performed at 90°C than when it was performed at 80, 70, and 50°C. This result is related to the diffusion coefficients of the polysaccharides, which increased from 1.07 × 10-12 at 50°C to 3.02 × 10 -12 m2/sec at 90°C. Using the Arrhenius equation, the pre-exponential factor (D0) and the activation energy (Ea) for Spirulina polysaccharide Extraction were calculated as 7.958 × 10-9 m2/sec and 24.0 kJ/mol, respectively. Among the methods used for the separation of Spirulina polysaccharides, cetyltrimethylammonium bromide (CTAB, method I) and organic solvent (ethanol, in methods II and III) provided similar yields of polysaccharides. However, the separation of polysaccharides using an ultrafiltration (UF) process (method III) and ethanol precipitation was superior to separation via CTAB or vacuum rotary evaporation (method II). The use of a membrane with a molecular weight cut-off (MWCO) of 30 kDa and an area of 0.01 m2 at a feed pressure of 103 kPa with a mean permeate flux of 39.3 L/m2/h and a retention rate of 95% was optimal for the UF process. The addition of two volumes (v/v) of ethanol, which gave a total polysaccharide content of approximately 4% dry weight, was found to be most suitable for polysaccharide precipitation. The results of a Sepharose 6B column separation showed that the molecular weights of the polysaccharides in fractions I and II were 212 and 12.6 kDa, respectively. © 2014 The Korean Society for Biotechnology and Bioengineering and Springer-Verlag.
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Polysaccharide Extraction from Spirulina sp. and its antioxidant capacity
International Journal of Biological Macromolecules, 2013Co-Authors: Ratana Chaiklahan, Panya Triratana, Nattayaporn Chirasuwan, Veara Loha, Suvit Tia, Boosya BunnagAbstract:To optimize polysaccharide Extraction from Spirulina sp., the effect of solid-to-liquid ratio, Extraction Temperature and time were investigated using Box-Behnken experimental design and response surface methodology. The results showed that Extraction Temperature and solid-to-liquid ratio had a significant impact on the yield of polysaccharides. A polysaccharides yield of around 8.3% dry weight was obtained under the following optimized conditions: solid-to-liquid ratio of 1:45, Temperature of 90. °C, and time of 120. min. The polysaccharide extracts contained rhamnose, which accounted for 53% of the total sugars, with a phenolic content of 45. mg GAE/g sample. © 2013 Elsevier B.V.
Athanasia M Goula - One of the best experts on this subject based on the ideXlab platform.
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ultrasound assisted Extraction of pomegranate seed oil kinetic modeling
Journal of Food Engineering, 2013Co-Authors: Athanasia M GoulaAbstract:Abstract In this work, ultrasound-assisted Extraction was employed to extract oil from pomegranate seeds. Seed particle size, Extraction Temperature, solvent/solid ratio, amplitude level, and pulse duration/pulse interval ratio were the factors investigated with respect to Extraction yield using a central composite design. The optimum operating conditions were found to be: seed particle size, 0.2 mm; Extraction Temperature, 20 o C; solvent/solid ratio, 20/1; amplitude level, 60%; pulse duration/pulse interval ratio, 5/15. Under these optimized conditions, the predicted value for Extraction yield was 59.8%. A second-order kinetic model was successfully developed for describing the mechanism of ultrasound Extraction under different processing parameters.
Ratana Chaiklahan - One of the best experts on this subject based on the ideXlab platform.
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Effect of Extraction Temperature on the diffusion coefficient of polysaccharides from Spirulina and the optimal separation method
Biotechnology and Bioprocess Engineering, 2014Co-Authors: Ratana Chaiklahan, Panya Triratana, Nattayaporn Chirasuwan, Boosya BunnagAbstract:The Extraction Temperature had a significant impact on the concentration of polysaccharides derived from solid-liquid Extraction of Spirulina. The polysaccharide concentration was significantly higher when the Extraction was performed at 90°C than when it was performed at 80, 70, and 50°C. This result is related to the diffusion coefficients of the polysaccharides, which increased from 1.07 × 10−12 at 50°C to 3.02 × 10−12 m2/sec at 90°C. Using the Arrhenius equation, the pre-exponential factor (D0) and the activation energy (Ea) for Spirulina polysaccharide Extraction were calculated as 7.958 × 10−9 m2/sec and 24.0 kJ/mol, respectively. Among the methods used for the separation of Spirulina polysaccharides, cetyltrimethylammonium bromide (CTAB, method I) and organic solvent (ethanol, in methods II and III) provided similar yields of polysaccharides. However, the separation of polysaccharides using an ultrafiltration (UF) process (method III) and ethanol precipitation was superior to separation via CTAB or vacuum rotary evaporation (method II). The use of a membrane with a molecular weight cut-off (MWCO) of 30 kDa and an area of 0.01 m2 at a feed pressure of 103 kPa with a mean permeate flux of 39.3 L/m2/h and a retention rate of 95% was optimal for the UF process. The addition of two volumes (v/v) of ethanol, which gave a total polysaccharide content of approximately 4% dry weight, was found to be most suitable for polysaccharide precipitation. The results of a Sepharose 6B column separation showed that the molecular weights of the polysaccharides in fractions I and II were 212 and 12.6 kDa, respectively.
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Effect of Extraction Temperature on the diffusion coefficient of polysaccharides from Spirulina and the optimal separation method
Biotechnology and Bioprocess Engineering, 2014Co-Authors: Ratana Chaiklahan, Panya Triratana, Nattayaporn Chirasuwan, Suvit Tia, Boosya BunnagAbstract:The Extraction Temperature had a significant impact on the concentration of polysaccharides derived from solid-liquid Extraction of Spirulina. The polysaccharide concentration was significantly higher when the Extraction was performed at 90°C than when it was performed at 80, 70, and 50°C. This result is related to the diffusion coefficients of the polysaccharides, which increased from 1.07 × 10-12 at 50°C to 3.02 × 10 -12 m2/sec at 90°C. Using the Arrhenius equation, the pre-exponential factor (D0) and the activation energy (Ea) for Spirulina polysaccharide Extraction were calculated as 7.958 × 10-9 m2/sec and 24.0 kJ/mol, respectively. Among the methods used for the separation of Spirulina polysaccharides, cetyltrimethylammonium bromide (CTAB, method I) and organic solvent (ethanol, in methods II and III) provided similar yields of polysaccharides. However, the separation of polysaccharides using an ultrafiltration (UF) process (method III) and ethanol precipitation was superior to separation via CTAB or vacuum rotary evaporation (method II). The use of a membrane with a molecular weight cut-off (MWCO) of 30 kDa and an area of 0.01 m2 at a feed pressure of 103 kPa with a mean permeate flux of 39.3 L/m2/h and a retention rate of 95% was optimal for the UF process. The addition of two volumes (v/v) of ethanol, which gave a total polysaccharide content of approximately 4% dry weight, was found to be most suitable for polysaccharide precipitation. The results of a Sepharose 6B column separation showed that the molecular weights of the polysaccharides in fractions I and II were 212 and 12.6 kDa, respectively. © 2014 The Korean Society for Biotechnology and Bioengineering and Springer-Verlag.
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Polysaccharide Extraction from Spirulina sp. and its antioxidant capacity
International Journal of Biological Macromolecules, 2013Co-Authors: Ratana Chaiklahan, Panya Triratana, Nattayaporn Chirasuwan, Veara Loha, Suvit Tia, Boosya BunnagAbstract:To optimize polysaccharide Extraction from Spirulina sp., the effect of solid-to-liquid ratio, Extraction Temperature and time were investigated using Box-Behnken experimental design and response surface methodology. The results showed that Extraction Temperature and solid-to-liquid ratio had a significant impact on the yield of polysaccharides. A polysaccharides yield of around 8.3% dry weight was obtained under the following optimized conditions: solid-to-liquid ratio of 1:45, Temperature of 90. °C, and time of 120. min. The polysaccharide extracts contained rhamnose, which accounted for 53% of the total sugars, with a phenolic content of 45. mg GAE/g sample. © 2013 Elsevier B.V.
Hou Xujie - One of the best experts on this subject based on the ideXlab platform.
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optimization of Extraction process of crude polysaccharides from wild edible bachu mushroom by response surface methodology
Carbohydrate Polymers, 2008Co-Authors: Hou XujieAbstract:Abstract Polysaccharides production from wild edible BaChu mushroom was carried out using boiling water decoction. Response surface methodology (RSM), based on a five level, four variable central composite rotatable design (CCRD), was employed to obtain the best possible combination of Extraction Temperature, Extraction time, particle size and ratio of water to mushroom for maximum polysaccharides production. The optimum Extraction conditions were as follows: Extraction Temperature 94 °C, Extraction time 10 h, particle size 33 and ratio of water to mushroom 6. Under these conditions, the experimental yield was 8.73%, which is well matched with the predictive yield.
Panya Triratana - One of the best experts on this subject based on the ideXlab platform.
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Effect of Extraction Temperature on the diffusion coefficient of polysaccharides from Spirulina and the optimal separation method
Biotechnology and Bioprocess Engineering, 2014Co-Authors: Ratana Chaiklahan, Panya Triratana, Nattayaporn Chirasuwan, Boosya BunnagAbstract:The Extraction Temperature had a significant impact on the concentration of polysaccharides derived from solid-liquid Extraction of Spirulina. The polysaccharide concentration was significantly higher when the Extraction was performed at 90°C than when it was performed at 80, 70, and 50°C. This result is related to the diffusion coefficients of the polysaccharides, which increased from 1.07 × 10−12 at 50°C to 3.02 × 10−12 m2/sec at 90°C. Using the Arrhenius equation, the pre-exponential factor (D0) and the activation energy (Ea) for Spirulina polysaccharide Extraction were calculated as 7.958 × 10−9 m2/sec and 24.0 kJ/mol, respectively. Among the methods used for the separation of Spirulina polysaccharides, cetyltrimethylammonium bromide (CTAB, method I) and organic solvent (ethanol, in methods II and III) provided similar yields of polysaccharides. However, the separation of polysaccharides using an ultrafiltration (UF) process (method III) and ethanol precipitation was superior to separation via CTAB or vacuum rotary evaporation (method II). The use of a membrane with a molecular weight cut-off (MWCO) of 30 kDa and an area of 0.01 m2 at a feed pressure of 103 kPa with a mean permeate flux of 39.3 L/m2/h and a retention rate of 95% was optimal for the UF process. The addition of two volumes (v/v) of ethanol, which gave a total polysaccharide content of approximately 4% dry weight, was found to be most suitable for polysaccharide precipitation. The results of a Sepharose 6B column separation showed that the molecular weights of the polysaccharides in fractions I and II were 212 and 12.6 kDa, respectively.
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Effect of Extraction Temperature on the diffusion coefficient of polysaccharides from Spirulina and the optimal separation method
Biotechnology and Bioprocess Engineering, 2014Co-Authors: Ratana Chaiklahan, Panya Triratana, Nattayaporn Chirasuwan, Suvit Tia, Boosya BunnagAbstract:The Extraction Temperature had a significant impact on the concentration of polysaccharides derived from solid-liquid Extraction of Spirulina. The polysaccharide concentration was significantly higher when the Extraction was performed at 90°C than when it was performed at 80, 70, and 50°C. This result is related to the diffusion coefficients of the polysaccharides, which increased from 1.07 × 10-12 at 50°C to 3.02 × 10 -12 m2/sec at 90°C. Using the Arrhenius equation, the pre-exponential factor (D0) and the activation energy (Ea) for Spirulina polysaccharide Extraction were calculated as 7.958 × 10-9 m2/sec and 24.0 kJ/mol, respectively. Among the methods used for the separation of Spirulina polysaccharides, cetyltrimethylammonium bromide (CTAB, method I) and organic solvent (ethanol, in methods II and III) provided similar yields of polysaccharides. However, the separation of polysaccharides using an ultrafiltration (UF) process (method III) and ethanol precipitation was superior to separation via CTAB or vacuum rotary evaporation (method II). The use of a membrane with a molecular weight cut-off (MWCO) of 30 kDa and an area of 0.01 m2 at a feed pressure of 103 kPa with a mean permeate flux of 39.3 L/m2/h and a retention rate of 95% was optimal for the UF process. The addition of two volumes (v/v) of ethanol, which gave a total polysaccharide content of approximately 4% dry weight, was found to be most suitable for polysaccharide precipitation. The results of a Sepharose 6B column separation showed that the molecular weights of the polysaccharides in fractions I and II were 212 and 12.6 kDa, respectively. © 2014 The Korean Society for Biotechnology and Bioengineering and Springer-Verlag.
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Polysaccharide Extraction from Spirulina sp. and its antioxidant capacity
International Journal of Biological Macromolecules, 2013Co-Authors: Ratana Chaiklahan, Panya Triratana, Nattayaporn Chirasuwan, Veara Loha, Suvit Tia, Boosya BunnagAbstract:To optimize polysaccharide Extraction from Spirulina sp., the effect of solid-to-liquid ratio, Extraction Temperature and time were investigated using Box-Behnken experimental design and response surface methodology. The results showed that Extraction Temperature and solid-to-liquid ratio had a significant impact on the yield of polysaccharides. A polysaccharides yield of around 8.3% dry weight was obtained under the following optimized conditions: solid-to-liquid ratio of 1:45, Temperature of 90. °C, and time of 120. min. The polysaccharide extracts contained rhamnose, which accounted for 53% of the total sugars, with a phenolic content of 45. mg GAE/g sample. © 2013 Elsevier B.V.
