The Experts below are selected from a list of 75 Experts worldwide ranked by ideXlab platform
Lluis Tort - One of the best experts on this subject based on the ideXlab platform.
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comparison of two stunning slaughtering methods on stress response and quality indicators of european sea bass dicentrarchus labrax
Aquaculture, 2009Co-Authors: L Acerete, L Reig, D Alvarez, R Flos, Lluis TortAbstract:Abstract Two stunning/slaughtering methods, ice asphyxia and carbon dioxide narcosis, as common capture procedures in commercial farms, were compared with asphyxia without ice in order to assess their suitability as stunning methods for European sea bass. In this study we have analyzed the stunning effects on the stress response related to fish welfare, and on the meat quality. Sampling was performed 0, 2, 9, 24, 48, 72 and 96 h after death, and for all sampling times, rigor mortis assessment, muscle pH, Eye Refraction index and sensory evaluation were determined as quality parameters. Moreover, osmolality, glucose, lactate and cortisol were determined as stress indicators. The muscle pH values were significantly lower in CO 2 narcosis group from the first 9 h until the end of the study in comparison to the respective zero time. If we compared muscle pH values between each slaughtering method with the asphyxia group through sampling time, we observed statistically higher values in CO 2 and ice asphyxia throughout the study. The sensorial evaluation showed a progressive decrease in the three groups analysed versus the respective zero time, although the comparison with the control group showed statistical differences only at zero and nine hours sampling time. Regarding stress indicators, all of them showed significant higher levels after both slaughtering methods compared with the undisturbed group and significant lower levels if compared with the asphyxia group. Our results also show that asphyxia produced a five-fold increase in glucose levels whereas other methods studied only produced a 1.5–1.7-fold increase. Cortisol levels increased 8-fold after asphyxia whereas the other slaughtering methods provoked a 5-fold increase. In summary, the results indicate that both methods are suitable to process fish although the CO 2 treatment showed lower lactate and cortisol response compared to asphyxia in ice. In terms of quality, differences between methods are minor.
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comparison of methods for anaesthetizing senegal sole solea senegalensis before slaughter stress responses and final product quality
Aquaculture, 2007Co-Authors: L Reig, R Flos, Laia Ribas, Simon Mackenzie, Bruce A Barton, Lluis TortAbstract:The aim of this work was to evaluate several anaesthetization methods such as clove oil, hypothermia and asphyxia in Senegal sole (Solea senegalensis), a highly resistant species to hypoxic conditions. The overall intent of the study was to determine a method that minimizes pre mortem stress and maximizes final product quality in sole for market sale. Several pre and post mortem indicators were considered for this study, namely rigor mortis, muscle pH, ATP/IMP ratios, Eye Refraction index and sensorial attributes. In addition, the stress experienced by the fish pre mortem was assessed by measuring cortisol, glucose and osmolality levels in blood plasma just after death (post mortem). Based on these physiological indicators, the results revealed that of the three methods considered, 1 ml·L �1 clove oil was the best method for stunning sole because it not only ensures a good final quality
R Flos - One of the best experts on this subject based on the ideXlab platform.
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comparison of two stunning slaughtering methods on stress response and quality indicators of european sea bass dicentrarchus labrax
Aquaculture, 2009Co-Authors: L Acerete, L Reig, D Alvarez, R Flos, Lluis TortAbstract:Abstract Two stunning/slaughtering methods, ice asphyxia and carbon dioxide narcosis, as common capture procedures in commercial farms, were compared with asphyxia without ice in order to assess their suitability as stunning methods for European sea bass. In this study we have analyzed the stunning effects on the stress response related to fish welfare, and on the meat quality. Sampling was performed 0, 2, 9, 24, 48, 72 and 96 h after death, and for all sampling times, rigor mortis assessment, muscle pH, Eye Refraction index and sensory evaluation were determined as quality parameters. Moreover, osmolality, glucose, lactate and cortisol were determined as stress indicators. The muscle pH values were significantly lower in CO 2 narcosis group from the first 9 h until the end of the study in comparison to the respective zero time. If we compared muscle pH values between each slaughtering method with the asphyxia group through sampling time, we observed statistically higher values in CO 2 and ice asphyxia throughout the study. The sensorial evaluation showed a progressive decrease in the three groups analysed versus the respective zero time, although the comparison with the control group showed statistical differences only at zero and nine hours sampling time. Regarding stress indicators, all of them showed significant higher levels after both slaughtering methods compared with the undisturbed group and significant lower levels if compared with the asphyxia group. Our results also show that asphyxia produced a five-fold increase in glucose levels whereas other methods studied only produced a 1.5–1.7-fold increase. Cortisol levels increased 8-fold after asphyxia whereas the other slaughtering methods provoked a 5-fold increase. In summary, the results indicate that both methods are suitable to process fish although the CO 2 treatment showed lower lactate and cortisol response compared to asphyxia in ice. In terms of quality, differences between methods are minor.
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comparison of methods for anaesthetizing senegal sole solea senegalensis before slaughter stress responses and final product quality
Aquaculture, 2007Co-Authors: L Reig, R Flos, Laia Ribas, Simon Mackenzie, Bruce A Barton, Lluis TortAbstract:The aim of this work was to evaluate several anaesthetization methods such as clove oil, hypothermia and asphyxia in Senegal sole (Solea senegalensis), a highly resistant species to hypoxic conditions. The overall intent of the study was to determine a method that minimizes pre mortem stress and maximizes final product quality in sole for market sale. Several pre and post mortem indicators were considered for this study, namely rigor mortis, muscle pH, ATP/IMP ratios, Eye Refraction index and sensorial attributes. In addition, the stress experienced by the fish pre mortem was assessed by measuring cortisol, glucose and osmolality levels in blood plasma just after death (post mortem). Based on these physiological indicators, the results revealed that of the three methods considered, 1 ml·L �1 clove oil was the best method for stunning sole because it not only ensures a good final quality
L Reig - One of the best experts on this subject based on the ideXlab platform.
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comparison of two stunning slaughtering methods on stress response and quality indicators of european sea bass dicentrarchus labrax
Aquaculture, 2009Co-Authors: L Acerete, L Reig, D Alvarez, R Flos, Lluis TortAbstract:Abstract Two stunning/slaughtering methods, ice asphyxia and carbon dioxide narcosis, as common capture procedures in commercial farms, were compared with asphyxia without ice in order to assess their suitability as stunning methods for European sea bass. In this study we have analyzed the stunning effects on the stress response related to fish welfare, and on the meat quality. Sampling was performed 0, 2, 9, 24, 48, 72 and 96 h after death, and for all sampling times, rigor mortis assessment, muscle pH, Eye Refraction index and sensory evaluation were determined as quality parameters. Moreover, osmolality, glucose, lactate and cortisol were determined as stress indicators. The muscle pH values were significantly lower in CO 2 narcosis group from the first 9 h until the end of the study in comparison to the respective zero time. If we compared muscle pH values between each slaughtering method with the asphyxia group through sampling time, we observed statistically higher values in CO 2 and ice asphyxia throughout the study. The sensorial evaluation showed a progressive decrease in the three groups analysed versus the respective zero time, although the comparison with the control group showed statistical differences only at zero and nine hours sampling time. Regarding stress indicators, all of them showed significant higher levels after both slaughtering methods compared with the undisturbed group and significant lower levels if compared with the asphyxia group. Our results also show that asphyxia produced a five-fold increase in glucose levels whereas other methods studied only produced a 1.5–1.7-fold increase. Cortisol levels increased 8-fold after asphyxia whereas the other slaughtering methods provoked a 5-fold increase. In summary, the results indicate that both methods are suitable to process fish although the CO 2 treatment showed lower lactate and cortisol response compared to asphyxia in ice. In terms of quality, differences between methods are minor.
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comparison of methods for anaesthetizing senegal sole solea senegalensis before slaughter stress responses and final product quality
Aquaculture, 2007Co-Authors: L Reig, R Flos, Laia Ribas, Simon Mackenzie, Bruce A Barton, Lluis TortAbstract:The aim of this work was to evaluate several anaesthetization methods such as clove oil, hypothermia and asphyxia in Senegal sole (Solea senegalensis), a highly resistant species to hypoxic conditions. The overall intent of the study was to determine a method that minimizes pre mortem stress and maximizes final product quality in sole for market sale. Several pre and post mortem indicators were considered for this study, namely rigor mortis, muscle pH, ATP/IMP ratios, Eye Refraction index and sensorial attributes. In addition, the stress experienced by the fish pre mortem was assessed by measuring cortisol, glucose and osmolality levels in blood plasma just after death (post mortem). Based on these physiological indicators, the results revealed that of the three methods considered, 1 ml·L �1 clove oil was the best method for stunning sole because it not only ensures a good final quality
Osuagwu, Uchechukwu Levi - One of the best experts on this subject based on the ideXlab platform.
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Peripheral Refraction validity of the Shin-Nippon SRW5000 autorefractor
'Ovid Technologies (Wolters Kluwer Health)', 2016Co-Authors: Osuagwu, Uchechukwu Levi, Suheimat Marwan, Wolffsohn, James S., Atchison, David A.Abstract:PURPOSE: To investigate the operation of the Shin-Nippon/Grand Seiko autorefractor and whether higher-order aberrations affect its peripheral Refraction measurements. METHODS: Information on instrument design, together with parameters and equations used to obtain Refraction, was obtained from a patent. A model Eye simulating the operating principles was tested with an optical design program. Effects of induced defocus and astigmatism on the retinal image were used to calibrate the model Eye to match the patent equations. Coma and trefoil were added to assess their effects on the image. Peripheral Refraction of a physical model Eye was measured along four visual field meridians with the Shin-Nippon/Grand Seiko autorefractor SRW-5000 and a Hartmann-Shack aberrometer, and simulated autorefractor peripheral Refraction was derived using the Zernike coefficients from the aberrometer. RESULTS: In simulation, the autorefractor's square image was changed in size by defocus, into rectangles or parallelograms by astigmatism, and into irregular shapes by coma and trefoil. In the presence of 1.0 D oblique astigmatism, errors in Refraction were proportional to the higher-order aberrations, with up to 0.8 D sphere and 1.5 D cylinder for ±0.6 μm of coma or trefoil coefficients with a 5-mm-diameter pupil. For the physical model Eye, Refraction with the aberrometer was similar in all visual field meridians, but Refraction with the autorefractor changed more quickly along one oblique meridian and less quickly along the other oblique meridian than along the horizontal and vertical meridians. Simulations predicted that higher-order aberrations would affect Refraction in oblique meridians, and this was supported by the experimental measurements with the physical model Eye. CONCLUSIONS: The autorefractor's peripheral Refraction measurements are valid for horizontal and vertical field meridians, but not for oblique field meridians. Similar instruments must be validated before being adopted outside their design scope
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Peripheral Refraction validity of the Shin-Nippon SRW5000 autorefractor
'Ovid Technologies (Wolters Kluwer Health)', 2016Co-Authors: Osuagwu, Uchechukwu Levi, Suheimat Marwan, Wolffsohn James, Atchison DavidAbstract:Purpose: To investigate the operation of the Shin-Nippon/Grand Seiko autorefractor and whether higher-order aberrations affect its peripheral Refraction measurements. Methods: Information on instrument design, together with parameters and equations used to obtain Refraction, was obtained from a patent. A model Eye simulating the operating principles was tested with an optical design program. Effects of induced defocus and astigmatism on the retinal image were used to calibrate the model Eye to match the patent equations. Coma and trefoil were added to assess their effects on the image. Peripheral Refraction of a physical model Eye was measured along four visual field meridians with the Shin-Nippon SRW-5000 and a Hartmann-Shack aberrometer, and simulated autorefractor peripheral Refraction was derived using the Zernike coefficients from the aberrometer. Results: In simulation, the autorefractor’s square image was changed in size by defocus, into rectangles or parallelograms by astigmatism, and into irregular shapes by coma and trefoil. In the presence of 1.0 D oblique astigmatism, errors in Refraction were proportional to the higher-order aberrations, with up to 0.8 D sphere and 1.5 D cylinder for ±0.6 µm of coma or trefoil coefficients with a 5 mm diameter pupil. For the physical model Eye, Refraction with the aberrometer was similar in all visual field meridians, but Refraction with the autorefractor changed more quickly along one oblique meridian and less quickly along the other oblique meridian, than along the horizontal and vertical meridians. Simulations predicted that higher-order aberrations would affect Refraction in oblique meridians, and this was supported by experimental measurements with the physical model Eye. Conclusions: The autorefractor’s peripheral Refraction measurements are valid for horizontal and vertical field meridians, but not for oblique field meridians. Similar instruments must be validated before being adopted outside their design scope
L Acerete - One of the best experts on this subject based on the ideXlab platform.
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comparison of two stunning slaughtering methods on stress response and quality indicators of european sea bass dicentrarchus labrax
Aquaculture, 2009Co-Authors: L Acerete, L Reig, D Alvarez, R Flos, Lluis TortAbstract:Abstract Two stunning/slaughtering methods, ice asphyxia and carbon dioxide narcosis, as common capture procedures in commercial farms, were compared with asphyxia without ice in order to assess their suitability as stunning methods for European sea bass. In this study we have analyzed the stunning effects on the stress response related to fish welfare, and on the meat quality. Sampling was performed 0, 2, 9, 24, 48, 72 and 96 h after death, and for all sampling times, rigor mortis assessment, muscle pH, Eye Refraction index and sensory evaluation were determined as quality parameters. Moreover, osmolality, glucose, lactate and cortisol were determined as stress indicators. The muscle pH values were significantly lower in CO 2 narcosis group from the first 9 h until the end of the study in comparison to the respective zero time. If we compared muscle pH values between each slaughtering method with the asphyxia group through sampling time, we observed statistically higher values in CO 2 and ice asphyxia throughout the study. The sensorial evaluation showed a progressive decrease in the three groups analysed versus the respective zero time, although the comparison with the control group showed statistical differences only at zero and nine hours sampling time. Regarding stress indicators, all of them showed significant higher levels after both slaughtering methods compared with the undisturbed group and significant lower levels if compared with the asphyxia group. Our results also show that asphyxia produced a five-fold increase in glucose levels whereas other methods studied only produced a 1.5–1.7-fold increase. Cortisol levels increased 8-fold after asphyxia whereas the other slaughtering methods provoked a 5-fold increase. In summary, the results indicate that both methods are suitable to process fish although the CO 2 treatment showed lower lactate and cortisol response compared to asphyxia in ice. In terms of quality, differences between methods are minor.
