The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Pierre Dorny - One of the best experts on this subject based on the ideXlab platform.
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bovine fasciolosis in the human fasciolosis hyperendemic binh dinh province in central vietnam
Acta Tropica, 2011Co-Authors: Thanh Giang Thi Nguyen, T L H Tran, Nicolas Praet, Thanh Hoa Le, Jozef Vercruysse, Niko Speybroeck, Pierre DornyAbstract:A cross-sectional survey on bovine fasciolosis was conducted in the Binh Dinh province of Central Vietnam that was previously identified as hyperendemic for human fasciolosis. In Vietnam, both pure Fasciola gigantica and hybrid and/or introgressed populations of liverflukes bearing genetic material from both Fasciola hepatica and F. gigantica infect humans and animals. In this study, 825 cattle were randomly selected from 8 of the 11 provincial districts for faecal collection; blood samples were taken from 400 of these animals. Fasciola eggs and antibodies against Fasciola were detected by a quantitative sedimentation method and an Enzyme Linked Immunosorbent Assay, respectively. Overall, 54.9% of the animals were shedding Fasciola eggs while 72.2% were Fasciola seropositive. Animals under two years showed lower Fasciola infection rates than older animals. There were no differences in infection rates between districts. These results indicate a very high prevalence of Fasciola infections in cattle in Binh Dinh province. It is concluded that a fasciolosis control programme should be designed in this region aiming at reducing infection in both cattle and humans. (C) 2010 Elsevier B.V. All rights reserved.
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assessment of a 27 kda antigen in enzyme linked immunosorbent assay for the diagnosis of fasciolosis in vietnamese patients
Tropical Medicine & International Health, 2010Co-Authors: Thanh Giang Thi Nguyen, Tinh Doan, Thanh Hoa Le, Jozef Vercruysse, Pierre DornyAbstract:Fasciolosis has emerged as an important zoonotic disease in many parts of the world. In recent years, an increasing number of human cases were reported in Vietnam. In this study, the 27-kDa component protein from the excretory/secretory production of adult Fasciola gigantica, purified by high performance liquid chromatography, was assessed in an enzyme-linked immunosorbent assay (ELISA) to detect antibodies against Fasciola spp. for diagnosis of human fasciolosis. The ELISA showed a high sensitivity (100%) and specificity (97.67%) when tested on patients with fasciolosis, other parasitic infections, cholangiocarcinoma and on healthy controls. The assay was applied for diagnosis on 143 patients in the Viet Duc-Hanoi hospital who presented with clinical signs of liver disease and lesions in their livers as shown by imaging techniques. Antibodies were found in 37 (25.9%) of these patients, of whom only 3 shed Fasciola eggs in their stools (2.1%). The excellent response to triclabendazole treatment of 37 sero-positive patients confirmed the diagnosis of fasciolosis. This study demonstrated the diagnostic potential for human fasciolosis of the 27-kDa antigen ELISA. Fasciolosis should be considered in the differential diagnosis of hepatic disease in Vietnam.
Tariq Halasa - One of the best experts on this subject based on the ideXlab platform.
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Farm-level risk factors for Fasciola hepatica infection in Danish dairy cattle as evaluated by two diagnostic methods
Parasites & Vectors, 2017Co-Authors: Nao Takeuchi-storm, Matthew Denwood, Tina Vicky Alstrup Hansen, Tariq Halasa, Heidi Larsen Enemark, Jaap Boes, E Rattenborg, Stig Milan ThamsborgAbstract:Background The prevalence of bovine fasciolosis in Denmark is increasing but appropriate guidelines for control are currently lacking. In order to help develop a control strategy for liver fluke, a risk factor study of farm management factors was conducted and the utility of bulk tank milk (BTM ELISA) as a tool for diagnosis in Danish dairy cattle farms was assessed. Methods This case-control study aimed to identify farm-level risk factors for fasciolosis in Danish dairy farms (> 50 animals slaughtered in 2013) using two diagnostic methods: recordings of liver condemnation at slaughter, and farm-level Fasciola hepatica antibody levels in BTM. A case farm was defined as having a minimum of 3 incidents of liver condemnation due to liver fluke at slaughter (in any age group) during 2013, and control farms were located within 10 km of at least one case farm and had no history of liver condemnation due to liver fluke during 2011–2013. The selected farmers were interviewed over telephone about grazing and control practices, and BTM from these farms was collected and analysed by ELISA in 2014. The final complete dataset consisting of 131 case and 63 control farms was analysed using logistic regression. Results Heifers grazing on wet pastures, dry cows grazing on wet pastures, herd size, breed and concurrent beef cattle production were identified as risk factors associated with being classified as a case farm. With the categorised BTM ELISA result as the response variable, heifers grazing on wet pastures, dry cows grazing on wet pastures, and purchase of cows were identified as risk factors. Within the case and control groups, 74.8 and 12.7% of farms were positive for fasciolosis on BTM ELISA, respectively. The differences are likely to be related to the detection limit of the farm-level prevalence by the BTM ELISA test, time span between slaughter data and BTM, and the relatively low sensitivity of liver inspection at slaughter. Conclusions Control of bovine fasciolosis in Denmark should target heifers and dry cows through grazing management and appropriate anthelmintic treatment, and BTM ELISA can be a useful diagnostic tool for fasciolosis in Danish dairy farms.
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prevalence risk factors and spatial analysis of liver fluke infections in danish cattle herds
Parasites & Vectors, 2015Co-Authors: Abbey Olsen, K Frankena, Rene Bodker, Nils Toft, S M Thamsborg, Heidi L Enemark, Tariq HalasaAbstract:Background Fasciola hepatica, a trematode parasite (liver fluke), infects a wide range of host species causing fasciolosis. The disease is prevalent world-wide and causes considerable economic losses to the livestock industry. Fasciolosis is regarded as an emerging food-borne zoonosis. To promote awareness among farmers and to implement strategies to control the infection, this study examined the prevalence, spatial distribution and risk factors for F. hepatica infection in Danish cattle herds.
Qiang Xue - One of the best experts on this subject based on the ideXlab platform.
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case report surgical intervention for Fasciolopsis buski infection a literature review
American Journal of Tropical Medicine and Hygiene, 2020Co-Authors: Weimin Wang, Qiang XueAbstract:Fasciolopsis buski, also called the giant intestinal fluke, is the largest intestinal fluke of the zoonotic trematode parasites and found mainly in Southeast Asian countries, including China. Fasciolopsis buski infection was formerly a common health problem in many countries, but it is now rare. Typically, it can be cured by oral drugs, but some infected patients need surgical intervention because of the severity of their condition or because of an unclear diagnosis or even misdiagnosis. Here, we report a case of a 15-year-old girl from Guizhou Province, China, presenting with recurrent upper-middle abdominal pain that was misdiagnosed as a choledochal cyst. Through laparotomy combined with postoperative histopathological examination, the source of the pain was proven to be mechanical biliary obstruction caused by F. buski infection. In the past, mechanical obstruction, especially biliary obstruction, caused by F. buski infection leading to surgery was not uncommon, but it is very rare in modern society. Moreover, delayed treatment and misdiagnosis of parasitic infection can lead to severe consequences. Therefore, we reviewed the previous literature on F. buski infection treated by surgical operation and summarized the characteristics and therapeutic strategies of these cases to raise clinicians' awareness of this rare infection.
Veena Tandon - One of the best experts on this subject based on the ideXlab platform.
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de novo genome and transcriptome analyses provide insights into the biology of the trematode human parasite Fasciolopsis buski
PLOS ONE, 2018Co-Authors: Devendra Kumar Biswal, Tanmoy Roychowdhury, Priyatama Pandey, Veena TandonAbstract:Many trematode parasites cause infection in humans and are thought to be a major public health problem. Their ecological diversity in different regions provides challenging questions on evolution of these organisms. In this report, we perform transcriptome analysis of the giant intestinal fluke, Fasciolopsis buski, using next generation sequencing technology. Short read sequences derived from polyA containing RNA of this organism were assembled into 30,677 unigenes that led to the annotation of 12,380 genes. Annotation of the assembled transcripts enabled insight into processes and pathways in the intestinal fluke, such as RNAi pathway and energy metabolism. The expressed kinome of the organism was characterized by identifying all protein kinases. A rough draft genome assembly for Fasciolopsis buski is also reported herewith with SRA accessions for crosschecking the findings in the analyzed transcriptome data. Transcriptome data also helped us to identify some of the expressed transposable elements. Though many Long Interspersed elements (LINEs) were identified, only two Short Interspersed Elements (SINEs) were visible. Overall transcriptome and draft genome analysis of F. buski helped us to characterize some of its important biological characteristics and provided enormous resources for development of a suitable diagnostic system and anti-parasitic therapeutic molecules.
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complete mitochondrial genome annotation of the giant intestinal fluke Fasciolopsis buski indian isolate as revealed by ion torrent and illuminanext generation sequencing
Mitochondrial DNA Part B, 2016Co-Authors: Devendra Kumar Biswal, Ruchishree Konhar, Manish Debnath, Veena TandonAbstract:The complete mitogenome sequences of the intestinal fluke Fasciolopsis buski are presented for the first time in this study. It is 14,119 bp long and is thus the shortest trematode mitochondrial ge...
Santiago Mascoma - One of the best experts on this subject based on the ideXlab platform.
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anaemia in advanced chronic fasciolosis
Acta Tropica, 2008Co-Authors: M A Valero, Nuria Girones, M A Garciabodelon, Messaoud Khoubbane, Maria Victoria Periago, Manuel Fresno, Isabel Chicocalero, Santiago MascomaAbstract:Abstract The association between fasciolosis-induced anaemia and related factors has been quantified in a rodent model. Haematological parameters were analysed in Wistar rats at 20 and 60 weeks post-infection (p.i.). Pigment stones and bile specimens were collected. Serum IgG1, IgG2a and IgE were determined in rat serum samples. Cytokine levels have been correlated with haematological parameters. The screening for gastrointestinal bleeding was carried out. Bacteriological bile cultures revealed viable bacteria in 53.8% of specimens at 60 weeks p.i. The results show that the type of anaemia in fasciolosis might be considered a biomarker of the chronicity period of the disease, changing from normocytic to macrocytic in the early chronic period (20 weeks p.i.) and to microcytic in the advanced chronic period (60 weeks p.i.). Likewise, changing from normochromic in the early chronic period to hypochromic in the advanced chronic period. Multivariate analysis suggested an association between anaemia and the following factors: fluke burden, eggs per gram of faeces, body area of parasite, presence of blood in faeces, IgG1 and eosinophil levels, and % of splenic weight. Of all variables analysed, the fluke burden is the one which presents the highest anaemia risk, even exceeding the variable presence of blood in faeces. The development of anaemia appears to be complex and may involve multiple mechanisms. However, to the mechanisms that until now explain Fascioliosis-related anaemia (compensatory increase in erythrocyte production and a continuous drain on iron stores resulting from the parasites’ blood-sucking activities) the following causes ought to be added: haemolysis of red blood cells, the general effects of inflammation on erythropoiesis, concomitant parasitic and bacterial infections and pre-morbid nutritional abnormalities. Extrapolation to human fasciolosis is discussed. The results of the rodent model lead to the assumption that a high risk of anaemia in subjects with a heavy parasitic burden in human hyperendemic areas of fasciolosis is to be expected.
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high risk of bacterobilia in advanced experimental chronic fasciolosis
Acta Tropica, 2006Co-Authors: M A Valero, M A Garciabodelon, Miriam Navarro, Maria M Morales, P Mengual, Antonio Marcilla, J.l. Hernández, Santiago MascomaAbstract:Fasciolosis is recognized as an important human disease. Wistar rats experimentally infected with Fasciola hepatica were examined using data obtained in the advanced chronic state of the disease (200, 300 and 400 days post-infection, dpi). Pigment stones (PS) and bile specimens were collected. The same procedure was applied in control rats. Liver tests were determined using stored serum samples. Bacteriological bile culture revealed viable bacteria (Escherichia coli, 45% of cases, Enterococcus faecalis, 45% and Klebsiella pneumoniae, 10%). The presence of bacterobilia was associated with liver serum enzymes, including aspartate aminotransferase (AST or SGOT), alanine aminotransferase (ALT or SGPT), alkaline phosphatase (AP) and total bilirubin levels. Multivariate analysis suggested an association between bacterobilia and the following factors: duration of parasitic infection and intensity of parasitic infection supported the impression that the obstruction caused by advanced chronic fasciolosis in the rat may be related to biliary sepsis. Extrapolation to human infection in fasciolosis hyperendemic areas is discussed. In conclusion, the results of the rodent model should lead to a reconsideration of treatment features in human disease, i.e. therapeutic strategies should not only include a parasitic treatment but also consider the possibility of bacterial co-infection.
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validation of a mapping and prediction model for human fasciolosis transmission in andean very high altitude endemic areas using remote sensing data
Acta Tropica, 2001Co-Authors: Marius V Fuentes, John B Malone, Santiago MascomaAbstract:Abstract The present paper aims to validate the usefulness of the Normalized Difference Vegetation Index (NDVI) obtained by satellite remote sensing for the development of local maps of risk and for prediction of human fasciolosis in the Northern Bolivian Altiplano. The endemic area, which is located at very high altitudes (3800–4100 m) between Lake Titicaca and the valley of the city of La Paz, presents the highest prevalences and intensities of fasciolosis known in humans. NDVI images of 1.1 km resolution from the Advanced Very High Resolution Radiometer (AVHRR) sensor on board the National Oceanic and Atmospheric Administration (NOAA) series of environmental satellites appear to provide adequate information for a study area such as that of the Northern Bolivian Altiplano. The predictive value of the remotely sensed map based on NDVI data appears to be better than that from forecast indices based only on climatic data. A close correspondence was observed between real ranges of human fasciolosis prevalence at 13 localities of known prevalence rates and the predicted ranges of fasciolosis prevalence using NDVI maps. However, results based on NDVI map data predicted zones as risk areas where, in fact, field studies have demonstrated the absence of lymnaeid populations during snail surveys, corroborated by the absence of the parasite in humans and livestock. NDVI data maps represent a useful data component in long-term efforts to develop a comprehensive geographical information system control program model that accurately fits real epidemiological and transmission situations of human fasciolosis in high altitude endemic areas in Andean countries.