The Experts below are selected from a list of 75 Experts worldwide ranked by ideXlab platform
Dong Jianping - One of the best experts on this subject based on the ideXlab platform.
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Comprehensive analysis on clinical symptoms of bacillary dysentery by laboratory confirmation and clinical diagnosis in Haidian District in 2007.
Modern Preventive Medicine, 2010Co-Authors: Sun Peiyuan, Xu Jie, Dong JianpingAbstract:[Objective] To discuss the pathogenetic features,types of pathogenic bacteria and drug resistance of bacillary dysentery by laboratory confirmation and clinical diagnosis in Haidian District,and then provide evidence for rational drug using in clinical diagnosis.[Methods] Determinated pathogenic bacteria by Feces Culture,and identify the species,grouped and serotyped by biochemistry and serology.Investigated the clinical symptoms and analyzed its clinical manifestation and drug resistance.[Results] The major clinical manifestations of the confirmed bacillary dysentery were atypical.Among the confirmed bacillary dysentery patients,more women were infected than men,which was great different from the clinical gender ratio.Only one patient had the mucopurulent bloody Feces,which was one typical manifestation in bacillary dysentery.Most of the patients had watery Feces,paste Feces and loose Feces.46.15% of the patients had the symptom of tenesmus.One patient endured abdominal pain.The main manifestations were dull pain and colic.The mild cases of patients might have normal temperature.88.89% of the patients' total leukocyte increased.The detecting rate of Shigella was 6.28% ;while the detecting rate of other pathogenic bacteria was 9.18%.Shigellas were resistant to ampicillin,rifampicin,nalidixic acid and tetracycline.[Conclusion] The major clinical manifestations of the confirmed bacillary dysentery are atypical,which are difficult to distinguish from other pathogenic bacteria infection.At least there are 9.18% of the clinical confirmed bacillary dysentery misdiagnosed.We should make comprehensive diagnosis by the symptoms,manifestation,temperature and laboratory test.Shigellas have various drug resistances towards different antibiotics,and thus we should strengthen monitoring.
D Owen Rae - One of the best experts on this subject based on the ideXlab platform.
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Lymphatic fluid for the detection of Mycobacterium avium subsp. paratuberculosis in cows by PCR, compared to fecal sampling and detection of antibodies in blood and milk.
Veterinary microbiology, 2014Co-Authors: Johannes L Khol, Pablo J Pinedo, Claus D Buergelt, Laura M Neumann, D Owen RaeAbstract:Johne's disease (JD), caused by Mycobacterium avium subsp. paratuberculosis (MAP), can cause considerable economic losses in affected herds. Early diagnosis of JD is hampered by the chronic nature of the disease with a slow subclincal progression. The aim of the present study was to challenge the hypothesis that lymphatic fluid is of diagnostic value in the early stages of the disease. Lymphatic fluid from 122 animals was collected and tested for MAP by nested PCR for IS900 and compared to the results of testing for MAP in Feces (Culture), blood and milk (ELISA) in 110 of these samples. MAP was detected by PCR in 27.1% of the lymph samples. Agreement between the tests was poor: 6.9% of the lymph positive cows were also positive in all other tests applied, and 69.0% had negative results in fecal Culture, blood and milk ELISA. Resampling of 25 cows after 8 to 12 and 16 to 20 months revealed 20.0% lymph positive animals at the first, 5.5% at the second and 27.8% at the third sampling, respectively. Only one cow showed positive lymph-PCR results at more than one sampling date. Lymph-positive cows had a 7.2 times greater likelihood of being culled within 8 to 12 months after sampling, compared to negative cows, mainly due to other health issues than JD. It can be concluded, that lymphatic fluid might be promising for the detection of early MAP-infection in cows, but further studies to elucidate the potential of this diagnostic approach are needed.
Wang Gui-qiang - One of the best experts on this subject based on the ideXlab platform.
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Infectious diarrhea bacterial pathogens monitoring and resistance to antibiotics in Beijing
The Chinese Journal of Clinical Pharmacology, 2008Co-Authors: Wang Gui-qiangAbstract:Objective To investigate the distribution, the resistance of bacterial pathogens isolated from acute diarrheal patients in Beijing. Methods Enteric pathogenic bacteria were isolated by Feces Culture. Antimicrobial susceptibility were carried out in Kirby-bauer method.Results Shigella spp.(23.4%) was still the most frequently detected pathogen in all of the pathogens resulted in diarrhea. The rate of conditional pathogenic bacteria such as Bacterium termo, Enterococci, and so on increased to 32.4%. The rate of resistence of bacterial pathogens often caused diarrhea to both ampicllin and trimethoprim-sulfamethoxazole were within 40%～100%, while to fluoroquinolones were less than 15%(excepted for Saimonella 20%), and to the third generation of cephalosporins were about 10%.Conclusion The rate of conditional pathogenic bacteria increased,which should be taken seriously . The rates of resistence of all pathogens caused diarrhea to fluoroquinolones were less than 20%, so can be remained as first-line medicines for infectious diarrhea.
Sun Peiyuan - One of the best experts on this subject based on the ideXlab platform.
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Comprehensive analysis on clinical symptoms of bacillary dysentery by laboratory confirmation and clinical diagnosis in Haidian District in 2007.
Modern Preventive Medicine, 2010Co-Authors: Sun Peiyuan, Xu Jie, Dong JianpingAbstract:[Objective] To discuss the pathogenetic features,types of pathogenic bacteria and drug resistance of bacillary dysentery by laboratory confirmation and clinical diagnosis in Haidian District,and then provide evidence for rational drug using in clinical diagnosis.[Methods] Determinated pathogenic bacteria by Feces Culture,and identify the species,grouped and serotyped by biochemistry and serology.Investigated the clinical symptoms and analyzed its clinical manifestation and drug resistance.[Results] The major clinical manifestations of the confirmed bacillary dysentery were atypical.Among the confirmed bacillary dysentery patients,more women were infected than men,which was great different from the clinical gender ratio.Only one patient had the mucopurulent bloody Feces,which was one typical manifestation in bacillary dysentery.Most of the patients had watery Feces,paste Feces and loose Feces.46.15% of the patients had the symptom of tenesmus.One patient endured abdominal pain.The main manifestations were dull pain and colic.The mild cases of patients might have normal temperature.88.89% of the patients' total leukocyte increased.The detecting rate of Shigella was 6.28% ;while the detecting rate of other pathogenic bacteria was 9.18%.Shigellas were resistant to ampicillin,rifampicin,nalidixic acid and tetracycline.[Conclusion] The major clinical manifestations of the confirmed bacillary dysentery are atypical,which are difficult to distinguish from other pathogenic bacteria infection.At least there are 9.18% of the clinical confirmed bacillary dysentery misdiagnosed.We should make comprehensive diagnosis by the symptoms,manifestation,temperature and laboratory test.Shigellas have various drug resistances towards different antibiotics,and thus we should strengthen monitoring.
Wayne R. Matson - One of the best experts on this subject based on the ideXlab platform.
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A carbon column-based liquid chromatography electrochemical approach to routine 8-hydroxy-2'-deoxyguanosine measurements in urine and other biologic matrices: a one-year evaluation of methods.
Free Radical Biology and Medicine, 1999Co-Authors: Mikhail B. Bogdanov, M. Flint Beal, Douglas R Mccabe, Reg M Griffin, Wayne R. MatsonAbstract:Abstract 8-Hydroxy-2′-deoxyguanosine (8OH2′dG) is a principal stable marker of hydroxyl radical damage to DNA. It has been related to a wide variety of disorders and environmental insults, and has been proposed as a useful systematic marker of oxidative stress. Analytic procedures for 8OH2′dG in DNA digests are well established; however, routine measurement of free 8OH2′dG in other body fluids such as urine or plasma has been problematic. This has hindered its evaluation as a general clinical, therapeutic monitoring, or environmental assessment tool. Therefore, we developed a liquid chromatography electrochemical column–switching system based on the use of the unique purine selectivity of porous carbon columns that allows routine accurate measurement of 8OH2′dG in a variety of biologic matrices. This paper describes the rationale of the system design and the protocols developed for 8OH2′dG in urine, plasma, cerebrospinal fluid, tissue, DNA, saliva, sweat, kidney dialysis fluid, foods, Feces, Culture matrix, and microdialysates. Concentrations in both human and animal body fluids and tissues are reported. The system performance is discussed in the context of a 1-year evaluation of the methods applied to approximately 3600 samples, using internal quality control and external blind testing to determine long-term accuracy. The methods are reliable and accurate, and therefore should prove useful in assessing the role and utility of oxidative DNA damage in aging and human illness.