The Experts below are selected from a list of 107370 Experts worldwide ranked by ideXlab platform
A E Lewtabor - One of the best experts on this subject based on the ideXlab platform.
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rhipicephalus boophilus microplus tick in vitro Feeding Methods for functional dsrna and vaccine candidate antibody screening
Ticks and Tick-borne Diseases, 2014Co-Authors: A E Lewtabor, A Bruyeres, Bing Zhang, Manuel Rodriguez ValleAbstract:Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) ticks cause economic losses for cattle industries throughout tropical and subtropical regions of the world estimated at $US2.5 billion annually. Lack of access to efficacious long-lasting vaccination regimes and increases in tick acaricide resistance have led to the investigation of targets for the development of novel tick vaccines and treatments. In vitro tick Feeding has been used for many tick species to study the effect of new acaricides on the transmission of tick-borne pathogens. Few studies have reported the use of in vitro Feeding for functional genomic studies using RNA interference and/or the effect of specific anti-tick antibodies. In particular, in vitro Feeding reports for the cattle tick are limited due to its relatively short hypostome. Previously published Methods were further modified to broaden optimal tick sizes/weights, Feeding sources including bovine and ovine serum, optimisation of commercially available blood anti-coagulant tubes, and IgG concentrations for effective antibody delivery. Ticks are fed overnight and monitored for ∼5–6 weeks to determine egg output and success of larval emergence using a humidified incubator. Lithium-heparin blood tubes provided the most reliable anti-coagulant for bovine blood Feeding compared with commercial citrated (CPDA) and EDTA tubes. Although >30 mg semi-engorged ticks fed more reliably, ticks as small as 15 mg also fed to repletion to lay viable eggs. Ticks which gained less than ∼10 mg during in vitro Feeding typically did not lay eggs. One mg/ml IgG from Bm86-vaccinated cattle produced a potent anti-tick effect in vitro (83% efficacy) similar to that observed in vivo. Alternatively, Feeding of dsRNA targeting Bm86 did not demonstrate anti-tick effects (11% efficacy) compared with the potent effects of ubiquitin dsRNA. This study optimises R. microplus tick in vitro Feeding Methods which support the development of cattle tick vaccines and treatments.
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rhipicephalus boophilus microplus tick in vitro Feeding Methods for functional dsrna and vaccine candidate antibody screening
Ticks and Tick-borne Diseases, 2014Co-Authors: A E Lewtabor, A Bruyeres, Bing Zhang, Manuel Rodriguez ValleAbstract:Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) ticks cause economic losses for cattle industries throughout tropical and subtropical regions of the world estimated at $US2.5 billion annually. Lack of access to efficacious long-lasting vaccination regimes and increases in tick acaricide resistance have led to the investigation of targets for the development of novel tick vaccines and treatments. In vitro tick Feeding has been used for many tick species to study the effect of new acaricides on the transmission of tick-borne pathogens. Few studies have reported the use of in vitro Feeding for functional genomic studies using RNA interference and/or the effect of specific anti-tick antibodies. In particular, in vitro Feeding reports for the cattle tick are limited due to its relatively short hypostome. Previously published Methods were further modified to broaden optimal tick sizes/weights, Feeding sources including bovine and ovine serum, optimisation of commercially available blood anti-coagulant tubes, and IgG concentrations for effective antibody delivery. Ticks are fed overnight and monitored for ∼5–6 weeks to determine egg output and success of larval emergence using a humidified incubator. Lithium-heparin blood tubes provided the most reliable anti-coagulant for bovine blood Feeding compared with commercial citrated (CPDA) and EDTA tubes. Although >30 mg semi-engorged ticks fed more reliably, ticks as small as 15 mg also fed to repletion to lay viable eggs. Ticks which gained less than ∼10 mg during in vitro Feeding typically did not lay eggs. One mg/ml IgG from Bm86-vaccinated cattle produced a potent anti-tick effect in vitro (83% efficacy) similar to that observed in vivo. Alternatively, Feeding of dsRNA targeting Bm86 did not demonstrate anti-tick effects (11% efficacy) compared with the potent effects of ubiquitin dsRNA. This study optimises R. microplus tick in vitro Feeding Methods which support the development of cattle tick vaccines and treatments.
Manuel Rodriguez Valle - One of the best experts on this subject based on the ideXlab platform.
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rhipicephalus boophilus microplus tick in vitro Feeding Methods for functional dsrna and vaccine candidate antibody screening
Ticks and Tick-borne Diseases, 2014Co-Authors: A E Lewtabor, A Bruyeres, Bing Zhang, Manuel Rodriguez ValleAbstract:Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) ticks cause economic losses for cattle industries throughout tropical and subtropical regions of the world estimated at $US2.5 billion annually. Lack of access to efficacious long-lasting vaccination regimes and increases in tick acaricide resistance have led to the investigation of targets for the development of novel tick vaccines and treatments. In vitro tick Feeding has been used for many tick species to study the effect of new acaricides on the transmission of tick-borne pathogens. Few studies have reported the use of in vitro Feeding for functional genomic studies using RNA interference and/or the effect of specific anti-tick antibodies. In particular, in vitro Feeding reports for the cattle tick are limited due to its relatively short hypostome. Previously published Methods were further modified to broaden optimal tick sizes/weights, Feeding sources including bovine and ovine serum, optimisation of commercially available blood anti-coagulant tubes, and IgG concentrations for effective antibody delivery. Ticks are fed overnight and monitored for ∼5–6 weeks to determine egg output and success of larval emergence using a humidified incubator. Lithium-heparin blood tubes provided the most reliable anti-coagulant for bovine blood Feeding compared with commercial citrated (CPDA) and EDTA tubes. Although >30 mg semi-engorged ticks fed more reliably, ticks as small as 15 mg also fed to repletion to lay viable eggs. Ticks which gained less than ∼10 mg during in vitro Feeding typically did not lay eggs. One mg/ml IgG from Bm86-vaccinated cattle produced a potent anti-tick effect in vitro (83% efficacy) similar to that observed in vivo. Alternatively, Feeding of dsRNA targeting Bm86 did not demonstrate anti-tick effects (11% efficacy) compared with the potent effects of ubiquitin dsRNA. This study optimises R. microplus tick in vitro Feeding Methods which support the development of cattle tick vaccines and treatments.
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rhipicephalus boophilus microplus tick in vitro Feeding Methods for functional dsrna and vaccine candidate antibody screening
Ticks and Tick-borne Diseases, 2014Co-Authors: A E Lewtabor, A Bruyeres, Bing Zhang, Manuel Rodriguez ValleAbstract:Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) ticks cause economic losses for cattle industries throughout tropical and subtropical regions of the world estimated at $US2.5 billion annually. Lack of access to efficacious long-lasting vaccination regimes and increases in tick acaricide resistance have led to the investigation of targets for the development of novel tick vaccines and treatments. In vitro tick Feeding has been used for many tick species to study the effect of new acaricides on the transmission of tick-borne pathogens. Few studies have reported the use of in vitro Feeding for functional genomic studies using RNA interference and/or the effect of specific anti-tick antibodies. In particular, in vitro Feeding reports for the cattle tick are limited due to its relatively short hypostome. Previously published Methods were further modified to broaden optimal tick sizes/weights, Feeding sources including bovine and ovine serum, optimisation of commercially available blood anti-coagulant tubes, and IgG concentrations for effective antibody delivery. Ticks are fed overnight and monitored for ∼5–6 weeks to determine egg output and success of larval emergence using a humidified incubator. Lithium-heparin blood tubes provided the most reliable anti-coagulant for bovine blood Feeding compared with commercial citrated (CPDA) and EDTA tubes. Although >30 mg semi-engorged ticks fed more reliably, ticks as small as 15 mg also fed to repletion to lay viable eggs. Ticks which gained less than ∼10 mg during in vitro Feeding typically did not lay eggs. One mg/ml IgG from Bm86-vaccinated cattle produced a potent anti-tick effect in vitro (83% efficacy) similar to that observed in vivo. Alternatively, Feeding of dsRNA targeting Bm86 did not demonstrate anti-tick effects (11% efficacy) compared with the potent effects of ubiquitin dsRNA. This study optimises R. microplus tick in vitro Feeding Methods which support the development of cattle tick vaccines and treatments.
A Bruyeres - One of the best experts on this subject based on the ideXlab platform.
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rhipicephalus boophilus microplus tick in vitro Feeding Methods for functional dsrna and vaccine candidate antibody screening
Ticks and Tick-borne Diseases, 2014Co-Authors: A E Lewtabor, A Bruyeres, Bing Zhang, Manuel Rodriguez ValleAbstract:Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) ticks cause economic losses for cattle industries throughout tropical and subtropical regions of the world estimated at $US2.5 billion annually. Lack of access to efficacious long-lasting vaccination regimes and increases in tick acaricide resistance have led to the investigation of targets for the development of novel tick vaccines and treatments. In vitro tick Feeding has been used for many tick species to study the effect of new acaricides on the transmission of tick-borne pathogens. Few studies have reported the use of in vitro Feeding for functional genomic studies using RNA interference and/or the effect of specific anti-tick antibodies. In particular, in vitro Feeding reports for the cattle tick are limited due to its relatively short hypostome. Previously published Methods were further modified to broaden optimal tick sizes/weights, Feeding sources including bovine and ovine serum, optimisation of commercially available blood anti-coagulant tubes, and IgG concentrations for effective antibody delivery. Ticks are fed overnight and monitored for ∼5–6 weeks to determine egg output and success of larval emergence using a humidified incubator. Lithium-heparin blood tubes provided the most reliable anti-coagulant for bovine blood Feeding compared with commercial citrated (CPDA) and EDTA tubes. Although >30 mg semi-engorged ticks fed more reliably, ticks as small as 15 mg also fed to repletion to lay viable eggs. Ticks which gained less than ∼10 mg during in vitro Feeding typically did not lay eggs. One mg/ml IgG from Bm86-vaccinated cattle produced a potent anti-tick effect in vitro (83% efficacy) similar to that observed in vivo. Alternatively, Feeding of dsRNA targeting Bm86 did not demonstrate anti-tick effects (11% efficacy) compared with the potent effects of ubiquitin dsRNA. This study optimises R. microplus tick in vitro Feeding Methods which support the development of cattle tick vaccines and treatments.
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rhipicephalus boophilus microplus tick in vitro Feeding Methods for functional dsrna and vaccine candidate antibody screening
Ticks and Tick-borne Diseases, 2014Co-Authors: A E Lewtabor, A Bruyeres, Bing Zhang, Manuel Rodriguez ValleAbstract:Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) ticks cause economic losses for cattle industries throughout tropical and subtropical regions of the world estimated at $US2.5 billion annually. Lack of access to efficacious long-lasting vaccination regimes and increases in tick acaricide resistance have led to the investigation of targets for the development of novel tick vaccines and treatments. In vitro tick Feeding has been used for many tick species to study the effect of new acaricides on the transmission of tick-borne pathogens. Few studies have reported the use of in vitro Feeding for functional genomic studies using RNA interference and/or the effect of specific anti-tick antibodies. In particular, in vitro Feeding reports for the cattle tick are limited due to its relatively short hypostome. Previously published Methods were further modified to broaden optimal tick sizes/weights, Feeding sources including bovine and ovine serum, optimisation of commercially available blood anti-coagulant tubes, and IgG concentrations for effective antibody delivery. Ticks are fed overnight and monitored for ∼5–6 weeks to determine egg output and success of larval emergence using a humidified incubator. Lithium-heparin blood tubes provided the most reliable anti-coagulant for bovine blood Feeding compared with commercial citrated (CPDA) and EDTA tubes. Although >30 mg semi-engorged ticks fed more reliably, ticks as small as 15 mg also fed to repletion to lay viable eggs. Ticks which gained less than ∼10 mg during in vitro Feeding typically did not lay eggs. One mg/ml IgG from Bm86-vaccinated cattle produced a potent anti-tick effect in vitro (83% efficacy) similar to that observed in vivo. Alternatively, Feeding of dsRNA targeting Bm86 did not demonstrate anti-tick effects (11% efficacy) compared with the potent effects of ubiquitin dsRNA. This study optimises R. microplus tick in vitro Feeding Methods which support the development of cattle tick vaccines and treatments.
Bing Zhang - One of the best experts on this subject based on the ideXlab platform.
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rhipicephalus boophilus microplus tick in vitro Feeding Methods for functional dsrna and vaccine candidate antibody screening
Ticks and Tick-borne Diseases, 2014Co-Authors: A E Lewtabor, A Bruyeres, Bing Zhang, Manuel Rodriguez ValleAbstract:Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) ticks cause economic losses for cattle industries throughout tropical and subtropical regions of the world estimated at $US2.5 billion annually. Lack of access to efficacious long-lasting vaccination regimes and increases in tick acaricide resistance have led to the investigation of targets for the development of novel tick vaccines and treatments. In vitro tick Feeding has been used for many tick species to study the effect of new acaricides on the transmission of tick-borne pathogens. Few studies have reported the use of in vitro Feeding for functional genomic studies using RNA interference and/or the effect of specific anti-tick antibodies. In particular, in vitro Feeding reports for the cattle tick are limited due to its relatively short hypostome. Previously published Methods were further modified to broaden optimal tick sizes/weights, Feeding sources including bovine and ovine serum, optimisation of commercially available blood anti-coagulant tubes, and IgG concentrations for effective antibody delivery. Ticks are fed overnight and monitored for ∼5–6 weeks to determine egg output and success of larval emergence using a humidified incubator. Lithium-heparin blood tubes provided the most reliable anti-coagulant for bovine blood Feeding compared with commercial citrated (CPDA) and EDTA tubes. Although >30 mg semi-engorged ticks fed more reliably, ticks as small as 15 mg also fed to repletion to lay viable eggs. Ticks which gained less than ∼10 mg during in vitro Feeding typically did not lay eggs. One mg/ml IgG from Bm86-vaccinated cattle produced a potent anti-tick effect in vitro (83% efficacy) similar to that observed in vivo. Alternatively, Feeding of dsRNA targeting Bm86 did not demonstrate anti-tick effects (11% efficacy) compared with the potent effects of ubiquitin dsRNA. This study optimises R. microplus tick in vitro Feeding Methods which support the development of cattle tick vaccines and treatments.
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rhipicephalus boophilus microplus tick in vitro Feeding Methods for functional dsrna and vaccine candidate antibody screening
Ticks and Tick-borne Diseases, 2014Co-Authors: A E Lewtabor, A Bruyeres, Bing Zhang, Manuel Rodriguez ValleAbstract:Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) ticks cause economic losses for cattle industries throughout tropical and subtropical regions of the world estimated at $US2.5 billion annually. Lack of access to efficacious long-lasting vaccination regimes and increases in tick acaricide resistance have led to the investigation of targets for the development of novel tick vaccines and treatments. In vitro tick Feeding has been used for many tick species to study the effect of new acaricides on the transmission of tick-borne pathogens. Few studies have reported the use of in vitro Feeding for functional genomic studies using RNA interference and/or the effect of specific anti-tick antibodies. In particular, in vitro Feeding reports for the cattle tick are limited due to its relatively short hypostome. Previously published Methods were further modified to broaden optimal tick sizes/weights, Feeding sources including bovine and ovine serum, optimisation of commercially available blood anti-coagulant tubes, and IgG concentrations for effective antibody delivery. Ticks are fed overnight and monitored for ∼5–6 weeks to determine egg output and success of larval emergence using a humidified incubator. Lithium-heparin blood tubes provided the most reliable anti-coagulant for bovine blood Feeding compared with commercial citrated (CPDA) and EDTA tubes. Although >30 mg semi-engorged ticks fed more reliably, ticks as small as 15 mg also fed to repletion to lay viable eggs. Ticks which gained less than ∼10 mg during in vitro Feeding typically did not lay eggs. One mg/ml IgG from Bm86-vaccinated cattle produced a potent anti-tick effect in vitro (83% efficacy) similar to that observed in vivo. Alternatively, Feeding of dsRNA targeting Bm86 did not demonstrate anti-tick effects (11% efficacy) compared with the potent effects of ubiquitin dsRNA. This study optimises R. microplus tick in vitro Feeding Methods which support the development of cattle tick vaccines and treatments.
Wenhong Zhang - One of the best experts on this subject based on the ideXlab platform.
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generation of long laminar plasma jets at atmospheric pressure and effects of flow turbulence
Plasma Chemistry and Plasma Processing, 2001Co-Authors: Wenhua Zhang, Wenhong Zhang, Chengkang WuAbstract:Long, laminar plasma jets at atmospheric pressure of pure argon and a mixture of argon and nitrogen with jet length up to 45 times its diameter could be generated with a DC arc torch by restricting the movement of arc root in the torch channel. Effects of torch structure, gas Feeding, and characteristics of power supply on the length of plasma jets were experimentally examined. Plasma jets of considerable length and excellent stability could be obtained by regulating the generating parameters, including arc channel geometry, gas flow rate, and Feeding Methods, etc. Influence of flow turbulence at the torch nozzle exit on the temperature distribution of plasma jets was numerically simulated. The analysis indicated that laminar flow plasma with very low initial turbulent kinetic energy will produce a long jet with low axial temperature gradient. This kind of long laminar plasma jet could greatly improve the controllability for materials processing, compared with a short turbulent arc jet.
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generation of long laminar plasma jets at atmospheric pressure and effects of flow turbulence
Plasma Chemistry and Plasma Processing, 2001Co-Authors: Wenxia Pan, Wenhua Zhang, Wenhong ZhangAbstract:Long, laminar plasma jets at atmospheric pressure of pure argon and a mixture of argon and nitrogen with jet length up to 45 fi,Hes its diameter could be generated with a DC are torch by! restricting the movement of arc root in the torch channel. Effects of torch structure, gas Feeding, and characteristics of power supply on the length of plasma jets were experimentally examined. Plasma jets of considerable length and excellent stability could be obtained by regulating the generating parameters, including are channel geometry gas flow I ate, and Feeding Methods, etc. Influence of flow turbulence at the torch,nozzle exit on the temperature distribution of plasma jets was numerically simulated. The analysis indicated that laminar flow plasma with very low initial turbulent kinetic energy will produce a long jet, with low axial temperature gradient. This kind of long laminar plasma jet could greatly improve the controllability for materials processing, compared with a short turbulent are let.
