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Chiara Valtolina - One of the best experts on this subject based on the ideXlab platform.
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Gene expressions of de novo Hepatic lipogenesis in Feline Hepatic Lipidosis.
Journal of feline medicine and surgery, 2019Co-Authors: Chiara Valtolina, Robert P. Favier, J.h. Robben, Monique E Van Wolferen, Hedwig S. Kruitwagen, Ronald J. Corbee, Louis C. PenningAbstract:ObjectivesThe aim of this study was to evaluate if de novo Hepatic lipid synthesis contributes to fatty acid overload in the liver of cats with Feline Hepatic Lipidosis (FHL).MethodsLipogenic gene ...
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Pathophysiologic Aspects of Feline Hepatic Lipidosis
2019Co-Authors: Chiara ValtolinaAbstract:Feline Hepatic Lipidosis (FHL) is considered the most common hepatobiliary disease in cats. It is characterized by the excessive accumulation of fat, in the form of triglycerides, in more than 80% of the hepatocytes resulting in secondary impairment of liver function, and intraHepatic cholestasis. Feline Hepatic Lipidosis presents as an acute critical syndrome that can result in the animal’s death due to severe liver dysfunction and failure if therapeutic measures are not taken expeditiously. The pathophysiologic mechanisms leading to the accumulation of fat in hepatocytes and FHL are not yet completely understood. Most likely, they consist of disturbances in the pathways that regulate uptake, synthesis, degradation, and secretion of fatty acids. The aim of this thesis is to unravel the characteristics of the different pathways of lipid mobilisation, Hepatic lipid metabolism and de novo lipid synthesis in healthy cats and cats with FHL. The effect of sex hormones on the lipid metabolism of healthy cats, and the lipid profile and the activation of progenitor cells in cats affected with Hepatic Lipidosis have been evaluated. Considering the results from our studies and data derived from experimental studies of overweight cats undergoing rapid weight loss and induction of Hepatic Lipidosis, we can argue that both limitations in lipid mobilisation from the liver and increased de novo Hepatic lipid synthesis are unlikely to be important causes of Hepatic lipid accumulation in FHL. The most likely pathophysiologic pathway appears an insufficient increase in the rate of fatty-acid oxidation in the face of increased peripheral lipid mobilization.
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Immunohistochemical characterisation of the Hepatic stem cell niche in Feline Hepatic Lipidosis : a preliminary morphological study
Journal of feline medicine and surgery, 2018Co-Authors: Chiara Valtolina, Robert P. Favier, J.h. Robben, Jan Rothuizen, Guy C. M. Grinwis, Baukje A. Schotanus, Louis C. PenningAbstract:ObjectivesThe aim of this study was to describe the cellular and stromal components of the Hepatic progenitor cell niche in Feline Hepatic Lipidosis (FHL).MethodsImmunohistochemical staining for th...
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sex specific differences in Hepatic and plasma lipid profiles in healthy cats pre and post spaying and neutering relationship with Feline Hepatic Lipidosis
BMC Veterinary Research, 2017Co-Authors: Chiara Valtolina, Robert P. Favier, Arie B. Vaandrager, Maidina Tuohetahuntila, Anne Kummeling, Isabelle Jeusette, J Rothuizen, J.h. RobbenAbstract:A link between lipid metabolism and disease has been recognized in cats. Since Hepatic Lipidosis is a frequent disorder in cats, the aim of the current study was to evaluate liver and plasma lipid dimorphism in healthy cats and the effects of gonadectomy on lipid profiling. From six female and six male cats plasma and liver lipid profiles before and after spaying/neutering were assessed and compared to five cats (three neutered male and two spayed female) diagnosed with Hepatic Lipidosis. Intact female cats had a significantly lower level of plasma triacylglycerides (TAG) and a higher liver level of the long chain polyunsaturated fatty acid arachidonic acid (AA) compared to their neutered state. Both male and female cats with Lipidosis had a higher liver, but not plasma TAG level and an increased level of plasma and liver sphingomyelin compared to the healthy cats. Although lipid dimorphism in healthy cats resembles that of other species, intact female cats show differences in metabolic configuration that could predispose them to develop Hepatic Lipidosis. The increased sphingomyelin levels in cats with Lipidosis could suggest a potential role in the pathogenesis of Hepatic Lipidosis in cats.
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Sex specific differences in Hepatic and plasma lipid profiles in healthy cats pre and post spaying and neutering: relationship with Feline Hepatic Lipidosis
BMC Veterinary Research, 2017Co-Authors: Chiara Valtolina, Robert P. Favier, Jan Rothuizen, Arie B. Vaandrager, Maidina Tuohetahuntila, Anne Kummeling, Isabelle Jeusette, Joris H. RobbenAbstract:Background A link between lipid metabolism and disease has been recognized in cats. Since Hepatic Lipidosis is a frequent disorder in cats, the aim of the current study was to evaluate liver and plasma lipid dimorphism in healthy cats and the effects of gonadectomy on lipid profiling. From six female and six male cats plasma and liver lipid profiles before and after spaying/neutering were assessed and compared to five cats (three neutered male and two spayed female) diagnosed with Hepatic Lipidosis . Results Intact female cats had a significantly lower level of plasma triacylglycerides (TAG) and a higher liver level of the long chain polyunsaturated fatty acid arachidonic acid (AA) compared to their neutered state. Both male and female cats with Lipidosis had a higher liver, but not plasma TAG level and an increased level of plasma and liver sphingomyelin compared to the healthy cats. Conclusion Although lipid dimorphism in healthy cats resembles that of other species, intact female cats show differences in metabolic configuration that could predispose them to develop Hepatic Lipidosis. The increased sphingomyelin levels in cats with Lipidosis could suggest a potential role in the pathogenesis of Hepatic Lipidosis in cats.
Robert P. Favier - One of the best experts on this subject based on the ideXlab platform.
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Gene expressions of de novo Hepatic lipogenesis in Feline Hepatic Lipidosis.
Journal of feline medicine and surgery, 2019Co-Authors: Chiara Valtolina, Robert P. Favier, J.h. Robben, Monique E Van Wolferen, Hedwig S. Kruitwagen, Ronald J. Corbee, Louis C. PenningAbstract:ObjectivesThe aim of this study was to evaluate if de novo Hepatic lipid synthesis contributes to fatty acid overload in the liver of cats with Feline Hepatic Lipidosis (FHL).MethodsLipogenic gene ...
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Immunohistochemical characterisation of the Hepatic stem cell niche in Feline Hepatic Lipidosis : a preliminary morphological study
Journal of feline medicine and surgery, 2018Co-Authors: Chiara Valtolina, Robert P. Favier, J.h. Robben, Jan Rothuizen, Guy C. M. Grinwis, Baukje A. Schotanus, Louis C. PenningAbstract:ObjectivesThe aim of this study was to describe the cellular and stromal components of the Hepatic progenitor cell niche in Feline Hepatic Lipidosis (FHL).MethodsImmunohistochemical staining for th...
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sex specific differences in Hepatic and plasma lipid profiles in healthy cats pre and post spaying and neutering relationship with Feline Hepatic Lipidosis
BMC Veterinary Research, 2017Co-Authors: Chiara Valtolina, Robert P. Favier, Arie B. Vaandrager, Maidina Tuohetahuntila, Anne Kummeling, Isabelle Jeusette, J Rothuizen, J.h. RobbenAbstract:A link between lipid metabolism and disease has been recognized in cats. Since Hepatic Lipidosis is a frequent disorder in cats, the aim of the current study was to evaluate liver and plasma lipid dimorphism in healthy cats and the effects of gonadectomy on lipid profiling. From six female and six male cats plasma and liver lipid profiles before and after spaying/neutering were assessed and compared to five cats (three neutered male and two spayed female) diagnosed with Hepatic Lipidosis. Intact female cats had a significantly lower level of plasma triacylglycerides (TAG) and a higher liver level of the long chain polyunsaturated fatty acid arachidonic acid (AA) compared to their neutered state. Both male and female cats with Lipidosis had a higher liver, but not plasma TAG level and an increased level of plasma and liver sphingomyelin compared to the healthy cats. Although lipid dimorphism in healthy cats resembles that of other species, intact female cats show differences in metabolic configuration that could predispose them to develop Hepatic Lipidosis. The increased sphingomyelin levels in cats with Lipidosis could suggest a potential role in the pathogenesis of Hepatic Lipidosis in cats.
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Sex specific differences in Hepatic and plasma lipid profiles in healthy cats pre and post spaying and neutering: relationship with Feline Hepatic Lipidosis
BMC Veterinary Research, 2017Co-Authors: Chiara Valtolina, Robert P. Favier, Jan Rothuizen, Arie B. Vaandrager, Maidina Tuohetahuntila, Anne Kummeling, Isabelle Jeusette, Joris H. RobbenAbstract:Background A link between lipid metabolism and disease has been recognized in cats. Since Hepatic Lipidosis is a frequent disorder in cats, the aim of the current study was to evaluate liver and plasma lipid dimorphism in healthy cats and the effects of gonadectomy on lipid profiling. From six female and six male cats plasma and liver lipid profiles before and after spaying/neutering were assessed and compared to five cats (three neutered male and two spayed female) diagnosed with Hepatic Lipidosis . Results Intact female cats had a significantly lower level of plasma triacylglycerides (TAG) and a higher liver level of the long chain polyunsaturated fatty acid arachidonic acid (AA) compared to their neutered state. Both male and female cats with Lipidosis had a higher liver, but not plasma TAG level and an increased level of plasma and liver sphingomyelin compared to the healthy cats. Conclusion Although lipid dimorphism in healthy cats resembles that of other species, intact female cats show differences in metabolic configuration that could predispose them to develop Hepatic Lipidosis. The increased sphingomyelin levels in cats with Lipidosis could suggest a potential role in the pathogenesis of Hepatic Lipidosis in cats.
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Feline Hepatic Lipidosis
The Veterinary clinics of North America. Small animal practice, 2017Co-Authors: Chiara Valtolina, Robert P. FavierAbstract:Feline Hepatic Lipidosis (FHL) is a common and potentially fatal liver disorder. Although the pathophysiologic mechanisms of FHL remain elusive, there is an imbalance between the influx of fatty acids from peripheral fat stores into the liver, de novo liposynthesis, and the rate of Hepatic oxidation and dispersal of Hepatic TAG via excretion of very-low density lipoproteins. The diagnosis of FHL is based on anamnestic, clinical, and clinicopathologic findings, associated with diagnostic imaging of the liver, and cytology, or histological examination of liver biopsies. Fluid therapy, electrolyte correction and adequate early nutrition are essential components of the therapy for FHL.
Jan Rothuizen - One of the best experts on this subject based on the ideXlab platform.
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Immunohistochemical characterisation of the Hepatic stem cell niche in Feline Hepatic Lipidosis : a preliminary morphological study
Journal of feline medicine and surgery, 2018Co-Authors: Chiara Valtolina, Robert P. Favier, J.h. Robben, Jan Rothuizen, Guy C. M. Grinwis, Baukje A. Schotanus, Louis C. PenningAbstract:ObjectivesThe aim of this study was to describe the cellular and stromal components of the Hepatic progenitor cell niche in Feline Hepatic Lipidosis (FHL).MethodsImmunohistochemical staining for th...
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Sex specific differences in Hepatic and plasma lipid profiles in healthy cats pre and post spaying and neutering: relationship with Feline Hepatic Lipidosis
BMC Veterinary Research, 2017Co-Authors: Chiara Valtolina, Robert P. Favier, Jan Rothuizen, Arie B. Vaandrager, Maidina Tuohetahuntila, Anne Kummeling, Isabelle Jeusette, Joris H. RobbenAbstract:Background A link between lipid metabolism and disease has been recognized in cats. Since Hepatic Lipidosis is a frequent disorder in cats, the aim of the current study was to evaluate liver and plasma lipid dimorphism in healthy cats and the effects of gonadectomy on lipid profiling. From six female and six male cats plasma and liver lipid profiles before and after spaying/neutering were assessed and compared to five cats (three neutered male and two spayed female) diagnosed with Hepatic Lipidosis . Results Intact female cats had a significantly lower level of plasma triacylglycerides (TAG) and a higher liver level of the long chain polyunsaturated fatty acid arachidonic acid (AA) compared to their neutered state. Both male and female cats with Lipidosis had a higher liver, but not plasma TAG level and an increased level of plasma and liver sphingomyelin compared to the healthy cats. Conclusion Although lipid dimorphism in healthy cats resembles that of other species, intact female cats show differences in metabolic configuration that could predispose them to develop Hepatic Lipidosis. The increased sphingomyelin levels in cats with Lipidosis could suggest a potential role in the pathogenesis of Hepatic Lipidosis in cats.
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No up-regulation of the phosphatidylethanolamine N-methyltransferase pathway and choline production by sex hormones in cats
BMC Veterinary Research, 2015Co-Authors: Chiara Valtolina, Robert P. Favier, Arie B. Vaandrager, Joris H. Robben, Maidina Tuohetahuntila, Anne Kummeling, Isabelle Jeusette, Jan RothuizenAbstract:Background Feline Hepatic Lipidosis (FHL) is a common cholestatic disease affecting cats of any breed, age and sex. Both choline deficiency and low Hepatic phosphatidylethanolamine N-methyltransferase (PEMT) activity are associated with Hepatic Lipidosis (HL) in humans, mice and rats. The PEMT expression is known to be upregulated by oestrogens, protecting the females in these species from the development of HL when exposed to choline deficient diets. The aim of the present study was to evaluate the influence of sex hormones on choline synthesis via the PEMT pathway in healthy male and female cats before and after spaying/neutering, when fed a diet with recommended dietary choline content. Results From six female and six male cats PEMT activity was assayed directly in liver biopsies taken before and after spaying/neutering, and assessed indirectly by analyses of PEMT–specific Hepatic phosphatidylcholine (PC) species and plasma choline levels. Hepatic PEMT activity did not differ between intact female and male cats and no changes upon spaying/neutering were observed. Likewise, no significant differences in liver PC content and PEMT-specific polyunsaturated PC species were found between the sexes and before or after spaying/neutering. Conclusion These results suggest that choline synthesis in cats differs from what is observed in humans, mice and rats. The lack of evident influence of sex hormones on the PEMT pathway makes it unlikely that spaying/neutering predisposes cats for HL by causing PC deficiency as suggested in other species.
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No up-regulation of the phosphatidylethanolamine N-methyltransferase pathway and choline production by sex hormones in cats
BMC veterinary research, 2015Co-Authors: Chiara Valtolina, Robert P. Favier, J.h. Robben, Arie B. Vaandrager, Maidina Tuohetahuntila, Anne Kummeling, Isabelle Jeusette, Jan RothuizenAbstract:Feline Hepatic Lipidosis (FHL) is a common cholestatic disease affecting cats of any breed, age and sex. Both choline deficiency and low Hepatic phosphatidylethanolamine N-methyltransferase (PEMT) activity are associated with Hepatic Lipidosis (HL) in humans, mice and rats. The PEMT expression is known to be upregulated by oestrogens, protecting the females in these species from the development of HL when exposed to choline deficient diets. The aim of the present study was to evaluate the influence of sex hormones on choline synthesis via the PEMT pathway in healthy male and female cats before and after spaying/neutering, when fed a diet with recommended dietary choline content. From six female and six male cats PEMT activity was assayed directly in liver biopsies taken before and after spaying/neutering, and assessed indirectly by analyses of PEMT–specific Hepatic phosphatidylcholine (PC) species and plasma choline levels. Hepatic PEMT activity did not differ between intact female and male cats and no changes upon spaying/neutering were observed. Likewise, no significant differences in liver PC content and PEMT-specific polyunsaturated PC species were found between the sexes and before or after spaying/neutering. These results suggest that choline synthesis in cats differs from what is observed in humans, mice and rats. The lack of evident influence of sex hormones on the PEMT pathway makes it unlikely that spaying/neutering predisposes cats for HL by causing PC deficiency as suggested in other species.
J.h. Robben - One of the best experts on this subject based on the ideXlab platform.
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Gene expressions of de novo Hepatic lipogenesis in Feline Hepatic Lipidosis.
Journal of feline medicine and surgery, 2019Co-Authors: Chiara Valtolina, Robert P. Favier, J.h. Robben, Monique E Van Wolferen, Hedwig S. Kruitwagen, Ronald J. Corbee, Louis C. PenningAbstract:ObjectivesThe aim of this study was to evaluate if de novo Hepatic lipid synthesis contributes to fatty acid overload in the liver of cats with Feline Hepatic Lipidosis (FHL).MethodsLipogenic gene ...
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Immunohistochemical characterisation of the Hepatic stem cell niche in Feline Hepatic Lipidosis : a preliminary morphological study
Journal of feline medicine and surgery, 2018Co-Authors: Chiara Valtolina, Robert P. Favier, J.h. Robben, Jan Rothuizen, Guy C. M. Grinwis, Baukje A. Schotanus, Louis C. PenningAbstract:ObjectivesThe aim of this study was to describe the cellular and stromal components of the Hepatic progenitor cell niche in Feline Hepatic Lipidosis (FHL).MethodsImmunohistochemical staining for th...
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sex specific differences in Hepatic and plasma lipid profiles in healthy cats pre and post spaying and neutering relationship with Feline Hepatic Lipidosis
BMC Veterinary Research, 2017Co-Authors: Chiara Valtolina, Robert P. Favier, Arie B. Vaandrager, Maidina Tuohetahuntila, Anne Kummeling, Isabelle Jeusette, J Rothuizen, J.h. RobbenAbstract:A link between lipid metabolism and disease has been recognized in cats. Since Hepatic Lipidosis is a frequent disorder in cats, the aim of the current study was to evaluate liver and plasma lipid dimorphism in healthy cats and the effects of gonadectomy on lipid profiling. From six female and six male cats plasma and liver lipid profiles before and after spaying/neutering were assessed and compared to five cats (three neutered male and two spayed female) diagnosed with Hepatic Lipidosis. Intact female cats had a significantly lower level of plasma triacylglycerides (TAG) and a higher liver level of the long chain polyunsaturated fatty acid arachidonic acid (AA) compared to their neutered state. Both male and female cats with Lipidosis had a higher liver, but not plasma TAG level and an increased level of plasma and liver sphingomyelin compared to the healthy cats. Although lipid dimorphism in healthy cats resembles that of other species, intact female cats show differences in metabolic configuration that could predispose them to develop Hepatic Lipidosis. The increased sphingomyelin levels in cats with Lipidosis could suggest a potential role in the pathogenesis of Hepatic Lipidosis in cats.
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No up-regulation of the phosphatidylethanolamine N-methyltransferase pathway and choline production by sex hormones in cats
BMC veterinary research, 2015Co-Authors: Chiara Valtolina, Robert P. Favier, J.h. Robben, Arie B. Vaandrager, Maidina Tuohetahuntila, Anne Kummeling, Isabelle Jeusette, Jan RothuizenAbstract:Feline Hepatic Lipidosis (FHL) is a common cholestatic disease affecting cats of any breed, age and sex. Both choline deficiency and low Hepatic phosphatidylethanolamine N-methyltransferase (PEMT) activity are associated with Hepatic Lipidosis (HL) in humans, mice and rats. The PEMT expression is known to be upregulated by oestrogens, protecting the females in these species from the development of HL when exposed to choline deficient diets. The aim of the present study was to evaluate the influence of sex hormones on choline synthesis via the PEMT pathway in healthy male and female cats before and after spaying/neutering, when fed a diet with recommended dietary choline content. From six female and six male cats PEMT activity was assayed directly in liver biopsies taken before and after spaying/neutering, and assessed indirectly by analyses of PEMT–specific Hepatic phosphatidylcholine (PC) species and plasma choline levels. Hepatic PEMT activity did not differ between intact female and male cats and no changes upon spaying/neutering were observed. Likewise, no significant differences in liver PC content and PEMT-specific polyunsaturated PC species were found between the sexes and before or after spaying/neutering. These results suggest that choline synthesis in cats differs from what is observed in humans, mice and rats. The lack of evident influence of sex hormones on the PEMT pathway makes it unlikely that spaying/neutering predisposes cats for HL by causing PC deficiency as suggested in other species.
Penning, Louis C. - One of the best experts on this subject based on the ideXlab platform.
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Gene expressions of de novo Hepatic lipogenesis in Feline Hepatic Lipidosis
2020Co-Authors: Valtolina Chiara, Robben, Joris H., Van Wolferen, Monique E., Kruitwagen, Hedwig S., Corbee, Ronald J., Favier, Robert P., Penning, Louis C.Abstract:Objectives: The aim of this study was to evaluate if de novo Hepatic lipid synthesis contributes to fatty acid overload in the liver of cats with Feline Hepatic Lipidosis (FHL). Methods: Lipogenic gene expression of peroxisome proliferator-activated receptor-alpha (PPAR-α), peroxisome proliferator-activated receptor-gamma (PPAR-γ), fatty acid synthase (FASN) and sterol regulatory element-binding factor (SREBF1) were evaluated using quantitative RT-PCR in liver tissue of six cats with FHL and compared with the liver tissue of eight healthy cats. Results: In liver tissue PPAR-α, PPAR-γ and FASN mRNA expression levels were not significantly different (P >0.12, P >0.89 and P >0.5, respectively) in the FHL group compared with the control group. SREBF1 gene expression was downregulated around 10-fold in the FHL group vs the control group (P = 0.039). Conclusions and relevance: The downregulation of SREBF1 in the liver tissue of cats with FHL does not support the hypothesis that de novo lipogenesis in the liver is an important pathway of fatty acid accumulation in FHL
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Immunohistochemical characterisation of the Hepatic stem cell niche in Feline Hepatic Lipidosis : a preliminary morphological study
2019Co-Authors: Valtolina Chiara, Robben, Joris H., Favier, Robert P., Guy C. M. Grinwis, Rothuizen Jan, Schotanus, Baukje A, Penning, Louis C.Abstract:Objectives The aim of this study was to describe the cellular and stromal components of the Hepatic progenitor cell niche in Feline Hepatic Lipidosis (FHL). Methods Immunohistochemical staining for the progenitor/bile duct marker (K19), activated Kupffer cells (MAC387), myofibroblasts (alpha-smooth muscle actin [α-SMA]) and the extracellular matrix component laminin were used on seven liver biopsies of cats with FHL and three healthy cats. Double immunofluorescence stainings were performed to investigate co-localisation of different cell types in the Hepatic progenitor cell (HPC) niche. Results HPCs, Kupffer cells, myofibroblasts and laminin deposition were observed in the liver samples of FHL, although with variability in the expression and positivity of the different immunostainings between different samples. When compared with the unaffected cats where K19 positivity and minimal α-SMA and laminin positivity were seen mainly in the portal area, in the majority of FHL samples K19 and α-SMA-positive cells and laminin positivity were seen also in the periportal and parenchymatous area. MAC387-positive cells were present throughout the parenchyma. Conclusions and relevance This is a preliminary morphological study to describe the activation and co-localisation of components of the HPC niche in FHL. Although the HPC niche in FHL resembles that described in hepatopathies in dogs and in Feline lymphocytic cholangitis, the expression of K19, α-SMA, MAC387 and lamin is more variable in FHL, and a common pattern of activation could not be established. Nevertheless, when HPCs were activated, a spatial association between HPCs and their niche could be demonstrated
