The Experts below are selected from a list of 156 Experts worldwide ranked by ideXlab platform
Edward A. Hoover - One of the best experts on this subject based on the ideXlab platform.
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a retrospective examination of Feline Leukemia subgroup characterization viral interference assays to deep sequencing
Viruses, 2018Co-Authors: Elliott S Chiu, Edward A. Hoover, Sue VandewoudeAbstract:Feline Leukemia virus (FeLV) was the first Feline retrovirus discovered, and is associated with multiple fatal disease syndromes in cats, including lymphoma. The original research conducted on FeLV employed classical virological techniques. As methods have evolved to allow FeLV genetic characterization, investigators have continued to unravel the molecular pathology associated with this fascinating agent. In this review, we discuss how FeLV classification, transmission, and disease-inducing potential have been defined sequentially by viral interference assays, Sanger sequencing, PCR, and next-generation sequencing. In particular, we highlight the influences of endogenous FeLV and host genetics that represent FeLV research opportunities on the near horizon.
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Development and testing of an inactivated Feline Leukemia virus vaccine.
Seminars in veterinary medicine and surgery (small animal), 1995Co-Authors: Edward A. Hoover, James I. Mullins, Hsien-jue ChuAbstract:We assessed an inactivated whole virus Feline Leukemia virus (FeLV) vaccine developed from a molecularly cloned Feline Leukemia virus isolate (FeLV-61E-A) for its ability to protect cats against homologous and heterologous virulent virus challenge. The fractions of cats that resisted the induction of persistent viremia after FeLV challenge were the following: (1) FeLV-61E-A vaccine, 95%; (2) adjuvant controls, 26%; and (3) established commercial control FeLV vaccine, 35%. The pre-challenge mean neutralizing antibody titers for each group were (1) FeLV-61E-A vaccine, 1:43; (2) adjuvant controls,
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In vitro infection of human bone marrow by Feline Leukemia viruses.
Virology, 1993Co-Authors: Edward A. HooverAbstract:Four subgroup Feline Leukemia viruses (FeLV) were tested for their ability to infect primary cultures of human bone marrow in vitro. Using three sequential exposures to both concentrated and unconcentrated virus, FeLV infection of human bone marrow was documented by indirect immunofluorescence, ELISA, and polymerase chain reaction (PCR) assays. Based on comparison to known standards, it was estimated that between 5 and 10% of the bulk human marrow culture could be infected by FeLV. Further evidence for FeLV reverse transcription, integration, and expression was obtained using specific PCR assays.
Sue Vandewoude - One of the best experts on this subject based on the ideXlab platform.
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a retrospective examination of Feline Leukemia subgroup characterization viral interference assays to deep sequencing
Viruses, 2018Co-Authors: Elliott S Chiu, Edward A. Hoover, Sue VandewoudeAbstract:Feline Leukemia virus (FeLV) was the first Feline retrovirus discovered, and is associated with multiple fatal disease syndromes in cats, including lymphoma. The original research conducted on FeLV employed classical virological techniques. As methods have evolved to allow FeLV genetic characterization, investigators have continued to unravel the molecular pathology associated with this fascinating agent. In this review, we discuss how FeLV classification, transmission, and disease-inducing potential have been defined sequentially by viral interference assays, Sanger sequencing, PCR, and next-generation sequencing. In particular, we highlight the influences of endogenous FeLV and host genetics that represent FeLV research opportunities on the near horizon.
Stephen J Obrien - One of the best experts on this subject based on the ideXlab platform.
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genetic characterization of Feline Leukemia virus from florida panthers
Emerging Infectious Diseases, 2008Co-Authors: Meredith A. Brown, Mark W. Cunningham, Alfred L. Roca, Jennifer L. Troyer, Warren E. Johnson, Stephen J ObrienAbstract:From 2002 through 2005, an outbreak of Feline Leukemia virus (FeLV) occurred in Florida panthers (Puma concolor coryi). Clinical signs included lymphadenopathy, anemia, septicemia, and weight loss; 5 panthers died. Not associated with FeLV outcome were the genetic heritage of the panthers (pure Florida vs. Texas/Florida crosses) and co-infection with Feline immunodeficiency virus. Genetic analysis of panther FeLV, designated FeLV-Pco, determined that the outbreak likely came from 1 cross-species transmission from a domestic cat. The FeLV-Pco virus was closely related to the domestic cat exogenous FeLV-A subgroup in lacking recombinant segments derived from endogenous FeLV. FeLV-Pco sequences were most similar to the well-characterized FeLV-945 strain, which is highly virulent and strongly pathogenic in domestic cats because of unique long terminal repeat and envelope sequences. These unique features may also account for the severity of the outbreak after cross-species transmission to the panther.
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insertional polymorphisms of endogenous Feline Leukemia viruses
Journal of Virology, 2005Co-Authors: Alfred L. Roca, William G Nash, Joan C Menninger, William J Murphy, Stephen J ObrienAbstract:The number, chromosomal distribution, and insertional polymorphisms of endogenous Feline Leukemia viruses (enFeLVs) were determined in four domestic cats (Burmese, Egyptian Mau, Persian, and nonbreed) using fluorescent in situ hybridization and radiation hybrid mapping. Twenty-nine distinct enFeLV loci were detected across 12 of the 18 autosomes. Each cat carried enFeLV at only 9 to 16 of the loci, and many loci were heterozygous for presence of the provirus. Thus, an average of 19 autosomal copies of enFeLV were present per cat diploid genome. Only five of the autosomal enFeLV sites were present in all four cats, and at only one autosomal locus, B4q15, was enFeLV present in both homologues of all four cats. A single enFeLV occurred in the X chromosome of the Burmese cat, while three to five enFeLV proviruses occurred in each Y chromosome. The X chromosome and nine autosomal enFeLV loci were telomeric, suggesting that ectopic recombination between nonhomologous subtelomeres may contribute to enFeLV distribution. Since endogenous FeLVs may affect the infectiousness or pathogenicity of exogenous FeLVs, genomic variation in enFeLVs represents a candidate for genetic influences on FeLV leukemogenesis in cats.
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genomically intact endogenous Feline Leukemia viruses of recent origin
Journal of Virology, 2004Co-Authors: Alfred L. Roca, Jill Peconslattery, Stephen J ObrienAbstract:We isolated and sequenced two complete endogenous Feline Leukemia viruses (enFeLVs), designated enFeLV-AGTT and enFeLV-GGAG. In enFeLV-AGTT, the open reading frames are reminiscent of a functioning FeLV genome, and the 5' and 3' long terminal repeat sequences are identical. Neither endogenous provirus is genetically fixed in cats but polymorphic, with 8.9 and 15.2% prevalence for enFeLV-AGTT and enFeLV-GGAG, respectively, among a survey of domestic cats. Neither provirus was found in the genomes of related species of the Felis genus, previously shown to harbor enFeLVs. The absence of mutational divergence, polymorphic incidence in cats, and absence in related species suggest that these enFeLVs may have entered the germ line more recently than previously believed, perhaps coincident with domestication, and reopens the question of whether some enFeLVs might be replication competent.
Hans Lutz - One of the best experts on this subject based on the ideXlab platform.
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Survey of the Feline Leukemia virus infection status of cats in Southern Germany.
Journal of feline medicine and surgery, 2012Co-Authors: Theresa Englert, Hans Lutz, Carola Sauter-louis, Katrin HartmannAbstract:Most studies that investigate the prevalence of infections with Feline Leukemia virus (FeLV) are based on the detection of p27 antigen in blood, but they do not detect proviral DNA to identify the ...
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Inhibition of Feline Leukemia virus replication by the integrase inhibitor Raltegravir.
Veterinary microbiology, 2011Co-Authors: Valentino Cattori, Beatrice Weibel, Hans LutzAbstract:The oncogenic gammaretrovirus Feline Leukemia virus (FeLV) has been the leading cause of death among domestic cats until the introduction of efficient diagnostics and vaccines in the late 1980s. So far, no efficient treatment for viremic animals is available. Hence, use of the FeLV model to evaluate antiretroviral therapies applied to HIV is a timely task. The efficacy of the integrase inhibitor Raltegravir, which is widely used for the treatment of HIV in humans, has been assessed in vitro for the FeLV-A/Glasgow-1 strain. EC(50) values for FeLV-A inhibition in Feline cell lines are in the range of that observed for HIV and xenotropic murine Leukemia virus-related gammaretrovirus. Therefore, Raltegravir may be a potential therapeutical agent for felids with progressive FeLV infection.
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Association between endogenous Feline Leukemia virus loads and exogenous Feline Leukemia virus infection in domestic cats.
Virus research, 2008Co-Authors: Ravi Tandon, Hans Lutz, Valentino Cattori, Andrea C. Pepin, Barbara Riond, Marina L. Meli, Michael Mcdonald, Marcus G. Doherr, Regina Hofmann-lehmannAbstract:Recently, we demonstrated that endogenous Feline Leukemia virus (enFeLV) loads may vary among cats of different populations and that FeLV-infected cats have higher enFeLV loads than uninfected cats. Thus, we hypothesized that enFeLV might influence the pathogenesis and outcome of FeLV infection. No significant difference in the infection outcome (regressive versus progressive infection) was observed between groups of cats with high or low enFeLV loads following FeLV-A challenge. However, cats with high enFeLV loads showed higher viral replication (plasma viral RNA and p27 antigen levels) than cats with low enFeLV loads in the early phase of the infection. The enFeLV transcription level varied at different time points, but no clear-cut pattern was observed. In conclusion, our results demonstrated an association between enFeLV loads and FeLV replication but not outcome of infection. enFeLV should be considered as an important confounder in experimental FeLV infection or vaccination studies.
Elliott S Chiu - One of the best experts on this subject based on the ideXlab platform.
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a retrospective examination of Feline Leukemia subgroup characterization viral interference assays to deep sequencing
Viruses, 2018Co-Authors: Elliott S Chiu, Edward A. Hoover, Sue VandewoudeAbstract:Feline Leukemia virus (FeLV) was the first Feline retrovirus discovered, and is associated with multiple fatal disease syndromes in cats, including lymphoma. The original research conducted on FeLV employed classical virological techniques. As methods have evolved to allow FeLV genetic characterization, investigators have continued to unravel the molecular pathology associated with this fascinating agent. In this review, we discuss how FeLV classification, transmission, and disease-inducing potential have been defined sequentially by viral interference assays, Sanger sequencing, PCR, and next-generation sequencing. In particular, we highlight the influences of endogenous FeLV and host genetics that represent FeLV research opportunities on the near horizon.
