The Experts below are selected from a list of 255 Experts worldwide ranked by ideXlab platform
Ruiying Liang - One of the best experts on this subject based on the ideXlab platform.
-
cellular microrna mir 1343 5p modulates ifn i responses to facilitate Feline Panleukopenia virus replication by directly targeting irak1 gene
Veterinary Microbiology, 2020Co-Authors: Lingling Zhang, Jingjie Zhao, Zhian Zhai, Lin Liang, Ruiying LiangAbstract:Abstract Feline Panleukopenia is an acute, highly contagious, and fatal infectious disease caused by Feline Panleukopenia virus (FPV) and has led to severe consequences on pets, economically important animals, and the wildlife industry. MicroRNAs (miRNAs) play significant roles in the host-pathogen interaction by modulating cellular factors expression which are essential for viral replication or host innate immune response to infection. However, the role of host miRNA response in FPV infection remains to be discovered. In this study, we screened nine host miRNAs associated with FPV infection that were previously implicated in innate immunity or antiviral functions. We found that miR-1343-5p overexpression strongly promoted FPV-BJ04 genomic DNA. Subsequently, the expression of host miR-1343-5p was upregulated by FPV-BJ04 infection in vitro and in vivo. In addition, we demonstrated that miR-1343-5p was a negative regulator of the IFN-I signaling pathway, thereby promoting FPV infection. Bioinformatic analysis combined with molecular biological assay indicated that interleukin-1 receptor-associated kinase 1 (IRAK1) is a putative target of miR-1343-5p. Collectively, our findings emphasize the importance of miR-1343-5p in host defense against FPV, thus, enhancing our understanding of its pathogenic mechanism.
-
Cellular microRNA, miR-1343-5p, modulates IFN-I responses to facilitate Feline Panleukopenia virus replication by directly targeting IRAK1 gene
Veterinary Microbiology, 2020Co-Authors: Lingling Zhang, Jingjie Zhao, Zhian Zhai, Lin Liang, Ruiying LiangAbstract:Abstract Feline Panleukopenia is an acute, highly contagious, and fatal infectious disease caused by Feline Panleukopenia virus (FPV) and has led to severe consequences on pets, economically important animals, and the wildlife industry. MicroRNAs (miRNAs) play significant roles in the host-pathogen interaction by modulating cellular factors expression which are essential for viral replication or host innate immune response to infection. However, the role of host miRNA response in FPV infection remains to be discovered. In this study, we screened nine host miRNAs associated with FPV infection that were previously implicated in innate immunity or antiviral functions. We found that miR-1343-5p overexpression strongly promoted FPV-BJ04 genomic DNA. Subsequently, the expression of host miR-1343-5p was upregulated by FPV-BJ04 infection in vitro and in vivo. In addition, we demonstrated that miR-1343-5p was a negative regulator of the IFN-I signaling pathway, regulating in the augment of FPV infection. Bioinformatic analysis combined with molecular biological assay indicated that interleukin-1 receptor-associated kinase 1 (IRAK1) is a putative target of miR-1343-5p. Collectively, our findings emphasize the importance of miR-1343-5p in host defense against FPV, thus, enhancing our understanding of its pathogenic mechanism.
-
regional adaptations and parallel mutations in Feline Panleukopenia virus strains from china revealed by nearly full length genome analysis
PLOS ONE, 2020Co-Authors: Elcio Leal, Lin Liang, Ruiying Liang, Jinxiang Li, Fabiola Villanova, Steven S WitkinAbstract:Protoparvoviruses, widespread among cats and wild animals, are responsible for leukopenia. Feline Panleukopenia virus (FPLV) in domestic cats is genetically diverse and some strains may differ from those used for vaccination. The presence of FPLV in two domestic cats from Hebei Province in China was identified by polymerase chain reaction. Samples from these animals were used to isolate FPLV strains in CRFK cells for genome sequencing. Phylogenetic analysis was performed to compare our isolates with available sequences of FPLV, mink parvovirus (MEV) and canine parvovirus (CPV). The isolated strains were closely related to strains of FPLV/MEV isolated in the 1960s. Our analysis also revealed that the evolutionary history of FPLV and MEV is characterized by local adaptations in the Vp2 gene. Thus, it is likely that new FPLV strains are emerging to evade the anti-FPLV immune response.
-
analysis of the microrna expression profiles in Feline kidney cell line infected with Feline Panleukopenia virus
Infection Genetics and Evolution, 2019Co-Authors: Lingling Zhang, Zhian Zhai, Ruiying Liang, Guangzhi Zhang, Yong Deng, Jinxiang LiAbstract:Abstract MicroRNAs (miRNAs) play crucial roles in post-transcriptional regulation of gene expression in many biological processes. Feline Panleukopenia virus (FPV) is a highly infectious pathogen that can cause severe disease in pets, economically important animals and wildlife. In this study, miRNAs associated with FPV infection were identified using high-throughput sequencing. Our results showed that 673 known miRNAs and 278 novel miRNAs were identified and 57 significantly differential expression miRNAs were found post-FPV infection in Feline kidney cell line. Stem-loop qRT-PCR was applied to validate the expression of the randomly selected miRNAs; the results were consistent with the sequencing data. Furthermore, the target genes of differential expression miRNAs were analyzed and predicated by GO and KEGG pathway. Altogether, our analysis provides a potential link between miRNA expression and the pathogenesis of FPV infection.
Lingling Zhang - One of the best experts on this subject based on the ideXlab platform.
-
cellular microrna mir 1343 5p modulates ifn i responses to facilitate Feline Panleukopenia virus replication by directly targeting irak1 gene
Veterinary Microbiology, 2020Co-Authors: Lingling Zhang, Jingjie Zhao, Zhian Zhai, Lin Liang, Ruiying LiangAbstract:Abstract Feline Panleukopenia is an acute, highly contagious, and fatal infectious disease caused by Feline Panleukopenia virus (FPV) and has led to severe consequences on pets, economically important animals, and the wildlife industry. MicroRNAs (miRNAs) play significant roles in the host-pathogen interaction by modulating cellular factors expression which are essential for viral replication or host innate immune response to infection. However, the role of host miRNA response in FPV infection remains to be discovered. In this study, we screened nine host miRNAs associated with FPV infection that were previously implicated in innate immunity or antiviral functions. We found that miR-1343-5p overexpression strongly promoted FPV-BJ04 genomic DNA. Subsequently, the expression of host miR-1343-5p was upregulated by FPV-BJ04 infection in vitro and in vivo. In addition, we demonstrated that miR-1343-5p was a negative regulator of the IFN-I signaling pathway, thereby promoting FPV infection. Bioinformatic analysis combined with molecular biological assay indicated that interleukin-1 receptor-associated kinase 1 (IRAK1) is a putative target of miR-1343-5p. Collectively, our findings emphasize the importance of miR-1343-5p in host defense against FPV, thus, enhancing our understanding of its pathogenic mechanism.
-
Cellular microRNA, miR-1343-5p, modulates IFN-I responses to facilitate Feline Panleukopenia virus replication by directly targeting IRAK1 gene
Veterinary Microbiology, 2020Co-Authors: Lingling Zhang, Jingjie Zhao, Zhian Zhai, Lin Liang, Ruiying LiangAbstract:Abstract Feline Panleukopenia is an acute, highly contagious, and fatal infectious disease caused by Feline Panleukopenia virus (FPV) and has led to severe consequences on pets, economically important animals, and the wildlife industry. MicroRNAs (miRNAs) play significant roles in the host-pathogen interaction by modulating cellular factors expression which are essential for viral replication or host innate immune response to infection. However, the role of host miRNA response in FPV infection remains to be discovered. In this study, we screened nine host miRNAs associated with FPV infection that were previously implicated in innate immunity or antiviral functions. We found that miR-1343-5p overexpression strongly promoted FPV-BJ04 genomic DNA. Subsequently, the expression of host miR-1343-5p was upregulated by FPV-BJ04 infection in vitro and in vivo. In addition, we demonstrated that miR-1343-5p was a negative regulator of the IFN-I signaling pathway, regulating in the augment of FPV infection. Bioinformatic analysis combined with molecular biological assay indicated that interleukin-1 receptor-associated kinase 1 (IRAK1) is a putative target of miR-1343-5p. Collectively, our findings emphasize the importance of miR-1343-5p in host defense against FPV, thus, enhancing our understanding of its pathogenic mechanism.
-
analysis of the microrna expression profiles in Feline kidney cell line infected with Feline Panleukopenia virus
Infection Genetics and Evolution, 2019Co-Authors: Lingling Zhang, Zhian Zhai, Ruiying Liang, Guangzhi Zhang, Yong Deng, Jinxiang LiAbstract:Abstract MicroRNAs (miRNAs) play crucial roles in post-transcriptional regulation of gene expression in many biological processes. Feline Panleukopenia virus (FPV) is a highly infectious pathogen that can cause severe disease in pets, economically important animals and wildlife. In this study, miRNAs associated with FPV infection were identified using high-throughput sequencing. Our results showed that 673 known miRNAs and 278 novel miRNAs were identified and 57 significantly differential expression miRNAs were found post-FPV infection in Feline kidney cell line. Stem-loop qRT-PCR was applied to validate the expression of the randomly selected miRNAs; the results were consistent with the sequencing data. Furthermore, the target genes of differential expression miRNAs were analyzed and predicated by GO and KEGG pathway. Altogether, our analysis provides a potential link between miRNA expression and the pathogenesis of FPV infection.
Lin Liang - One of the best experts on this subject based on the ideXlab platform.
-
cellular microrna mir 1343 5p modulates ifn i responses to facilitate Feline Panleukopenia virus replication by directly targeting irak1 gene
Veterinary Microbiology, 2020Co-Authors: Lingling Zhang, Jingjie Zhao, Zhian Zhai, Lin Liang, Ruiying LiangAbstract:Abstract Feline Panleukopenia is an acute, highly contagious, and fatal infectious disease caused by Feline Panleukopenia virus (FPV) and has led to severe consequences on pets, economically important animals, and the wildlife industry. MicroRNAs (miRNAs) play significant roles in the host-pathogen interaction by modulating cellular factors expression which are essential for viral replication or host innate immune response to infection. However, the role of host miRNA response in FPV infection remains to be discovered. In this study, we screened nine host miRNAs associated with FPV infection that were previously implicated in innate immunity or antiviral functions. We found that miR-1343-5p overexpression strongly promoted FPV-BJ04 genomic DNA. Subsequently, the expression of host miR-1343-5p was upregulated by FPV-BJ04 infection in vitro and in vivo. In addition, we demonstrated that miR-1343-5p was a negative regulator of the IFN-I signaling pathway, thereby promoting FPV infection. Bioinformatic analysis combined with molecular biological assay indicated that interleukin-1 receptor-associated kinase 1 (IRAK1) is a putative target of miR-1343-5p. Collectively, our findings emphasize the importance of miR-1343-5p in host defense against FPV, thus, enhancing our understanding of its pathogenic mechanism.
-
Cellular microRNA, miR-1343-5p, modulates IFN-I responses to facilitate Feline Panleukopenia virus replication by directly targeting IRAK1 gene
Veterinary Microbiology, 2020Co-Authors: Lingling Zhang, Jingjie Zhao, Zhian Zhai, Lin Liang, Ruiying LiangAbstract:Abstract Feline Panleukopenia is an acute, highly contagious, and fatal infectious disease caused by Feline Panleukopenia virus (FPV) and has led to severe consequences on pets, economically important animals, and the wildlife industry. MicroRNAs (miRNAs) play significant roles in the host-pathogen interaction by modulating cellular factors expression which are essential for viral replication or host innate immune response to infection. However, the role of host miRNA response in FPV infection remains to be discovered. In this study, we screened nine host miRNAs associated with FPV infection that were previously implicated in innate immunity or antiviral functions. We found that miR-1343-5p overexpression strongly promoted FPV-BJ04 genomic DNA. Subsequently, the expression of host miR-1343-5p was upregulated by FPV-BJ04 infection in vitro and in vivo. In addition, we demonstrated that miR-1343-5p was a negative regulator of the IFN-I signaling pathway, regulating in the augment of FPV infection. Bioinformatic analysis combined with molecular biological assay indicated that interleukin-1 receptor-associated kinase 1 (IRAK1) is a putative target of miR-1343-5p. Collectively, our findings emphasize the importance of miR-1343-5p in host defense against FPV, thus, enhancing our understanding of its pathogenic mechanism.
-
regional adaptations and parallel mutations in Feline Panleukopenia virus strains from china revealed by nearly full length genome analysis
PLOS ONE, 2020Co-Authors: Elcio Leal, Lin Liang, Ruiying Liang, Jinxiang Li, Fabiola Villanova, Steven S WitkinAbstract:Protoparvoviruses, widespread among cats and wild animals, are responsible for leukopenia. Feline Panleukopenia virus (FPLV) in domestic cats is genetically diverse and some strains may differ from those used for vaccination. The presence of FPLV in two domestic cats from Hebei Province in China was identified by polymerase chain reaction. Samples from these animals were used to isolate FPLV strains in CRFK cells for genome sequencing. Phylogenetic analysis was performed to compare our isolates with available sequences of FPLV, mink parvovirus (MEV) and canine parvovirus (CPV). The isolated strains were closely related to strains of FPLV/MEV isolated in the 1960s. Our analysis also revealed that the evolutionary history of FPLV and MEV is characterized by local adaptations in the Vp2 gene. Thus, it is likely that new FPLV strains are emerging to evade the anti-FPLV immune response.
Zhian Zhai - One of the best experts on this subject based on the ideXlab platform.
-
cellular microrna mir 1343 5p modulates ifn i responses to facilitate Feline Panleukopenia virus replication by directly targeting irak1 gene
Veterinary Microbiology, 2020Co-Authors: Lingling Zhang, Jingjie Zhao, Zhian Zhai, Lin Liang, Ruiying LiangAbstract:Abstract Feline Panleukopenia is an acute, highly contagious, and fatal infectious disease caused by Feline Panleukopenia virus (FPV) and has led to severe consequences on pets, economically important animals, and the wildlife industry. MicroRNAs (miRNAs) play significant roles in the host-pathogen interaction by modulating cellular factors expression which are essential for viral replication or host innate immune response to infection. However, the role of host miRNA response in FPV infection remains to be discovered. In this study, we screened nine host miRNAs associated with FPV infection that were previously implicated in innate immunity or antiviral functions. We found that miR-1343-5p overexpression strongly promoted FPV-BJ04 genomic DNA. Subsequently, the expression of host miR-1343-5p was upregulated by FPV-BJ04 infection in vitro and in vivo. In addition, we demonstrated that miR-1343-5p was a negative regulator of the IFN-I signaling pathway, thereby promoting FPV infection. Bioinformatic analysis combined with molecular biological assay indicated that interleukin-1 receptor-associated kinase 1 (IRAK1) is a putative target of miR-1343-5p. Collectively, our findings emphasize the importance of miR-1343-5p in host defense against FPV, thus, enhancing our understanding of its pathogenic mechanism.
-
Cellular microRNA, miR-1343-5p, modulates IFN-I responses to facilitate Feline Panleukopenia virus replication by directly targeting IRAK1 gene
Veterinary Microbiology, 2020Co-Authors: Lingling Zhang, Jingjie Zhao, Zhian Zhai, Lin Liang, Ruiying LiangAbstract:Abstract Feline Panleukopenia is an acute, highly contagious, and fatal infectious disease caused by Feline Panleukopenia virus (FPV) and has led to severe consequences on pets, economically important animals, and the wildlife industry. MicroRNAs (miRNAs) play significant roles in the host-pathogen interaction by modulating cellular factors expression which are essential for viral replication or host innate immune response to infection. However, the role of host miRNA response in FPV infection remains to be discovered. In this study, we screened nine host miRNAs associated with FPV infection that were previously implicated in innate immunity or antiviral functions. We found that miR-1343-5p overexpression strongly promoted FPV-BJ04 genomic DNA. Subsequently, the expression of host miR-1343-5p was upregulated by FPV-BJ04 infection in vitro and in vivo. In addition, we demonstrated that miR-1343-5p was a negative regulator of the IFN-I signaling pathway, regulating in the augment of FPV infection. Bioinformatic analysis combined with molecular biological assay indicated that interleukin-1 receptor-associated kinase 1 (IRAK1) is a putative target of miR-1343-5p. Collectively, our findings emphasize the importance of miR-1343-5p in host defense against FPV, thus, enhancing our understanding of its pathogenic mechanism.
-
analysis of the microrna expression profiles in Feline kidney cell line infected with Feline Panleukopenia virus
Infection Genetics and Evolution, 2019Co-Authors: Lingling Zhang, Zhian Zhai, Ruiying Liang, Guangzhi Zhang, Yong Deng, Jinxiang LiAbstract:Abstract MicroRNAs (miRNAs) play crucial roles in post-transcriptional regulation of gene expression in many biological processes. Feline Panleukopenia virus (FPV) is a highly infectious pathogen that can cause severe disease in pets, economically important animals and wildlife. In this study, miRNAs associated with FPV infection were identified using high-throughput sequencing. Our results showed that 673 known miRNAs and 278 novel miRNAs were identified and 57 significantly differential expression miRNAs were found post-FPV infection in Feline kidney cell line. Stem-loop qRT-PCR was applied to validate the expression of the randomly selected miRNAs; the results were consistent with the sequencing data. Furthermore, the target genes of differential expression miRNAs were analyzed and predicated by GO and KEGG pathway. Altogether, our analysis provides a potential link between miRNA expression and the pathogenesis of FPV infection.
Jingjie Zhao - One of the best experts on this subject based on the ideXlab platform.
-
cellular microrna mir 1343 5p modulates ifn i responses to facilitate Feline Panleukopenia virus replication by directly targeting irak1 gene
Veterinary Microbiology, 2020Co-Authors: Lingling Zhang, Jingjie Zhao, Zhian Zhai, Lin Liang, Ruiying LiangAbstract:Abstract Feline Panleukopenia is an acute, highly contagious, and fatal infectious disease caused by Feline Panleukopenia virus (FPV) and has led to severe consequences on pets, economically important animals, and the wildlife industry. MicroRNAs (miRNAs) play significant roles in the host-pathogen interaction by modulating cellular factors expression which are essential for viral replication or host innate immune response to infection. However, the role of host miRNA response in FPV infection remains to be discovered. In this study, we screened nine host miRNAs associated with FPV infection that were previously implicated in innate immunity or antiviral functions. We found that miR-1343-5p overexpression strongly promoted FPV-BJ04 genomic DNA. Subsequently, the expression of host miR-1343-5p was upregulated by FPV-BJ04 infection in vitro and in vivo. In addition, we demonstrated that miR-1343-5p was a negative regulator of the IFN-I signaling pathway, thereby promoting FPV infection. Bioinformatic analysis combined with molecular biological assay indicated that interleukin-1 receptor-associated kinase 1 (IRAK1) is a putative target of miR-1343-5p. Collectively, our findings emphasize the importance of miR-1343-5p in host defense against FPV, thus, enhancing our understanding of its pathogenic mechanism.
-
Cellular microRNA, miR-1343-5p, modulates IFN-I responses to facilitate Feline Panleukopenia virus replication by directly targeting IRAK1 gene
Veterinary Microbiology, 2020Co-Authors: Lingling Zhang, Jingjie Zhao, Zhian Zhai, Lin Liang, Ruiying LiangAbstract:Abstract Feline Panleukopenia is an acute, highly contagious, and fatal infectious disease caused by Feline Panleukopenia virus (FPV) and has led to severe consequences on pets, economically important animals, and the wildlife industry. MicroRNAs (miRNAs) play significant roles in the host-pathogen interaction by modulating cellular factors expression which are essential for viral replication or host innate immune response to infection. However, the role of host miRNA response in FPV infection remains to be discovered. In this study, we screened nine host miRNAs associated with FPV infection that were previously implicated in innate immunity or antiviral functions. We found that miR-1343-5p overexpression strongly promoted FPV-BJ04 genomic DNA. Subsequently, the expression of host miR-1343-5p was upregulated by FPV-BJ04 infection in vitro and in vivo. In addition, we demonstrated that miR-1343-5p was a negative regulator of the IFN-I signaling pathway, regulating in the augment of FPV infection. Bioinformatic analysis combined with molecular biological assay indicated that interleukin-1 receptor-associated kinase 1 (IRAK1) is a putative target of miR-1343-5p. Collectively, our findings emphasize the importance of miR-1343-5p in host defense against FPV, thus, enhancing our understanding of its pathogenic mechanism.
