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Luciane Holsback - One of the best experts on this subject based on the ideXlab platform.
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Natural infection by endoparasites among free-living Wild Animals Infecção natural por endoparasitas em animais silvestres de vida-livre
2020Co-Authors: Luciane Holsback, José Lahm Cardoso, Helena ConstantinoAbstract:Abstract The objective of this study was to investigate the frequency of occurrence and variety of intestinal parasites among free-living Wild Animals. Fecal samples from Wild mammals and birds at rehabilitation centers in the states of Mato Grosso do Sul and São Paulo were analyzed by sedimentation and flotation-centrifugation methods. Parasite eggs, oocysts, cysts and/or trophozoites were found in 71% of the samples. Cryptosporidium sp. oocysts were detected in fecal samples from oncillas (Leopardus tigrinus) and scaly-headed parrots (Pionus maximiliani). Giardia cysts were identified in the feces of a gray brocket (Mazama gouazoubira). Among the most common parasites found, there were eggs from Toxocara cati, Toxascaris leonina and Ancylostoma tubaeforme, and from Cestoda. Several Enterobius sp. eggs were found in the feces of red howler monkeys (Alouatta seniculus). It can be concluded from this study that despite the small number of samples, the diversity of parasites found was noteworthy. Additional information about parasite endofauna in Wild Animals is needed, since their presence might suggest that there could be proximity to and interactions with domestic Animals and/or humans. In addition, further studies on parasites from free-living Wild Animals are of prime importance for understanding the intensity of anthropic changes in Wild environments
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natural infection by endoparasites among free living Wild Animals
Revista Brasileira De Parasitologia Veterinaria, 2013Co-Authors: Luciane Holsback, Mauro José Lahm Cardoso, Rafael Fagnani, Thaís Helena Constantino PatelliAbstract:The objective of this study was to investigate the frequency of occurrence and variety of intestinal parasites among free-living Wild Animals. Fecal samples from Wild mammals and birds at rehabilitation centers in the states of Mato Grosso do Sul and Sao Paulo were analyzed by sedimentation and flotation-centrifugation methods. Parasite eggs, oocysts, cysts and/or trophozoites were found in 71% of the samples. Cryptosporidium sp. oocysts were detected in fecal samples from oncillas (Leopardus tigrinus) and scaly-headed parrots (Pionus maximiliani). Giardia cysts were identified in the feces of a gray brocket (Mazama gouazoubira). Among the most common parasites found, there were eggs from Toxocara cati, Toxascaris leonina and Ancylostoma tubaeforme, and from Cestoda. Several Enterobius sp. eggs were found in the feces of red howler monkeys (Alouatta seniculus). It can be concluded from this study that despite the small number of samples, the diversity of parasites found was noteworthy. Additional information about parasite endofauna in Wild Animals is needed, since their presence might suggest that there could be proximity to and interactions with domestic Animals and/or humans. In addition, further studies on parasites from free-living Wild Animals are of prime importance for understanding the intensity of anthropic changes in Wild environments.
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Natural infection by endoparasites among free-living Wild Animals Infecção natural por endoparasitas em animais silvestres de vida-livre
Colégio Brasileiro de Parasitologia Veterinaria, 2025Co-Authors: Luciane Holsback, Mauro José Lahm Cardoso, Rafael Fagnani, Thaís Helena Constantino PatelliAbstract:The objective of this study was to investigate the frequency of occurrence and variety of intestinal parasites among free-living Wild Animals. Fecal samples from Wild mammals and birds at rehabilitation centers in the states of Mato Grosso do Sul and São Paulo were analyzed by sedimentation and flotation-centrifugation methods. Parasite eggs, oocysts, cysts and/or trophozoites were found in 71% of the samples. Cryptosporidium sp. oocysts were detected in fecal samples from oncillas (Leopardus tigrinus) and scaly-headed parrots (Pionus maximiliani). Giardia cysts were identified in the feces of a gray brocket (Mazama gouazoubira). Among the most common parasites found, there were eggs from Toxocara cati, Toxascaris leonina and Ancylostoma tubaeforme, and from Cestoda. Several Enterobius sp. eggs were found in the feces of red howler monkeys (Alouatta seniculus). It can be concluded from this study that despite the small number of samples, the diversity of parasites found was noteworthy. Additional information about parasite endofauna in Wild Animals is needed, since their presence might suggest that there could be proximity to and interactions with domestic Animals and/or humans. In addition, further studies on parasites from free-living Wild Animals are of prime importance for understanding the intensity of anthropic changes in Wild environments.O objetivo deste trabalho foi pesquisar a frequência de ocorrência e a variedade de parasitas intestinais de animais silvestres de vida livre. Amostras de fezes de mamíferos e aves silvestres de centros de reabilitação dos Estados do Mato Grosso do Sul e São Paulo, foram analisadas pelos métodos de sedimentação e de centrífugo-flutuação. Foram encontrados ovos, oocistos, cistos e/ou trofozoítos de parasitas em 71% das amostras. Oocistos de Cryptosporidium sp. foram detectados em amostras de fezes de gato-do-mato-pequeno (Leopardus tigrinus) e maritacas (Pionus maximiliani). Cistos de Giardia foram identificados nas fezes de um veado catingueiro (Mazama gouazoubira). Entre os parasitas mais comuns, foram encontrados ovos de Toxocara cati, Toxascaris leonina e Ancylostoma tubaeforme, além de ovos de Cestoda. Vários ovos de Enterobius sp. foram encontrados nas fezes de bugio (Alouatta seniculus). Neste trabalho concluiu-se que, apesar de pequeno o número de amostras, a diversidade de parasitas encontrados foi relevante. Informações adicionais sobre a endofauna parasitária em animais silvestres são necessárias, pois podem sugerir a interação e proximidade com animais domésticos e/ou o homem. Além disso, maiores estudos sobre os parasitas de animais silvestres de vida livre são primordiais para a compreensão da intensidade de alterações antrópicas no meio silvestre
Thaís Helena Constantino Patelli - One of the best experts on this subject based on the ideXlab platform.
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natural infection by endoparasites among free living Wild Animals
Revista Brasileira De Parasitologia Veterinaria, 2013Co-Authors: Luciane Holsback, Mauro José Lahm Cardoso, Rafael Fagnani, Thaís Helena Constantino PatelliAbstract:The objective of this study was to investigate the frequency of occurrence and variety of intestinal parasites among free-living Wild Animals. Fecal samples from Wild mammals and birds at rehabilitation centers in the states of Mato Grosso do Sul and Sao Paulo were analyzed by sedimentation and flotation-centrifugation methods. Parasite eggs, oocysts, cysts and/or trophozoites were found in 71% of the samples. Cryptosporidium sp. oocysts were detected in fecal samples from oncillas (Leopardus tigrinus) and scaly-headed parrots (Pionus maximiliani). Giardia cysts were identified in the feces of a gray brocket (Mazama gouazoubira). Among the most common parasites found, there were eggs from Toxocara cati, Toxascaris leonina and Ancylostoma tubaeforme, and from Cestoda. Several Enterobius sp. eggs were found in the feces of red howler monkeys (Alouatta seniculus). It can be concluded from this study that despite the small number of samples, the diversity of parasites found was noteworthy. Additional information about parasite endofauna in Wild Animals is needed, since their presence might suggest that there could be proximity to and interactions with domestic Animals and/or humans. In addition, further studies on parasites from free-living Wild Animals are of prime importance for understanding the intensity of anthropic changes in Wild environments.
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Natural infection by endoparasites among free-living Wild Animals Infecção natural por endoparasitas em animais silvestres de vida-livre
Colégio Brasileiro de Parasitologia Veterinaria, 2025Co-Authors: Luciane Holsback, Mauro José Lahm Cardoso, Rafael Fagnani, Thaís Helena Constantino PatelliAbstract:The objective of this study was to investigate the frequency of occurrence and variety of intestinal parasites among free-living Wild Animals. Fecal samples from Wild mammals and birds at rehabilitation centers in the states of Mato Grosso do Sul and São Paulo were analyzed by sedimentation and flotation-centrifugation methods. Parasite eggs, oocysts, cysts and/or trophozoites were found in 71% of the samples. Cryptosporidium sp. oocysts were detected in fecal samples from oncillas (Leopardus tigrinus) and scaly-headed parrots (Pionus maximiliani). Giardia cysts were identified in the feces of a gray brocket (Mazama gouazoubira). Among the most common parasites found, there were eggs from Toxocara cati, Toxascaris leonina and Ancylostoma tubaeforme, and from Cestoda. Several Enterobius sp. eggs were found in the feces of red howler monkeys (Alouatta seniculus). It can be concluded from this study that despite the small number of samples, the diversity of parasites found was noteworthy. Additional information about parasite endofauna in Wild Animals is needed, since their presence might suggest that there could be proximity to and interactions with domestic Animals and/or humans. In addition, further studies on parasites from free-living Wild Animals are of prime importance for understanding the intensity of anthropic changes in Wild environments.O objetivo deste trabalho foi pesquisar a frequência de ocorrência e a variedade de parasitas intestinais de animais silvestres de vida livre. Amostras de fezes de mamíferos e aves silvestres de centros de reabilitação dos Estados do Mato Grosso do Sul e São Paulo, foram analisadas pelos métodos de sedimentação e de centrífugo-flutuação. Foram encontrados ovos, oocistos, cistos e/ou trofozoítos de parasitas em 71% das amostras. Oocistos de Cryptosporidium sp. foram detectados em amostras de fezes de gato-do-mato-pequeno (Leopardus tigrinus) e maritacas (Pionus maximiliani). Cistos de Giardia foram identificados nas fezes de um veado catingueiro (Mazama gouazoubira). Entre os parasitas mais comuns, foram encontrados ovos de Toxocara cati, Toxascaris leonina e Ancylostoma tubaeforme, além de ovos de Cestoda. Vários ovos de Enterobius sp. foram encontrados nas fezes de bugio (Alouatta seniculus). Neste trabalho concluiu-se que, apesar de pequeno o número de amostras, a diversidade de parasitas encontrados foi relevante. Informações adicionais sobre a endofauna parasitária em animais silvestres são necessárias, pois podem sugerir a interação e proximidade com animais domésticos e/ou o homem. Além disso, maiores estudos sobre os parasitas de animais silvestres de vida livre são primordiais para a compreensão da intensidade de alterações antrópicas no meio silvestre
Manuel Simões - One of the best experts on this subject based on the ideXlab platform.
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Biofilms and antibiotic susceptibility of multidrug-resistant bacteria from Wild Animals
PeerJ Inc., 2018Co-Authors: Carla Dias, Anabela Borges, Diana Oliveira, Antonio Martinez-murcia, Maria José Saavedra, Manuel SimõesAbstract:Background The “One Health” concept recognizes that human health and animal health are interdependent and bound to the health of the ecosystem in which they (co)exist. This interconnection favors the transmission of bacteria and other infectious agents as well as the flow of genetic elements containing antibiotic resistance genes. This problem is worsened when pathogenic bacteria have the ability to establish as biofilms. Therefore, it is important to understand the characteristics and behaviour of microorganisms in both planktonic and biofilms states from the most diverse environmental niches to mitigate the emergence and dissemination of resistance. Methods The purpose of this work was to assess the antibiotic susceptibility of four bacteria (Acinetobacter spp., Klebsiella pneumoniae, Pseudomonas fluorescens and Shewanella putrefaciens) isolated from Wild Animals and their ability to form biofilms. The effect of two antibiotics, imipenem (IPM) and ciprofloxacin (CIP), on biofilm removal was also assessed. Screening of resistance genetic determinants was performed by PCR. Biofilm tests were performed by a modified microtiter plate method. Bacterial surface hydrophobicity was determined by sessile drop contact angles. Results The susceptibility profile classified the bacteria as multidrug-resistant. Three genes coding for β-lactamases were detected in K. pneumoniae (TEM, SHV, OXA-aer) and one in P. fluorescens (OXA-aer). K. pneumoniae was the microorganism that carried more β-lactamase genes and it was the most proficient biofilm producer, while P. fluorescens demonstrated the highest adhesion ability. Antibiotics at their MIC, 5 × MIC and 10 × MIC were ineffective in total biofilm removal. The highest biomass reductions were found with IPM (54% at 10 × MIC) against K. pneumoniae biofilms and with CIP (40% at 10 × MIC) against P. fluorescens biofilms. Discussion The results highlight Wildlife as important host reservoirs and vectors for the spread of multidrug-resistant bacteria and genetic determinants of resistance. The ability of these bacteria to form biofilms should increase their persistence
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Biofilms and antibiotic susceptibility of multidrug-resistant bacteria from Wild Animals
'PeerJ', 2018Co-Authors: Carla Dias, Anabela Borges, Diana Oliveira, Antonio Martinez-murcia, Maria José Saavedra, Manuel SimõesAbstract:Background. The "One Health" concept recognizes that human health and animal health are interdependent and bound to the health of the ecosystem in which they (co)exist. This interconnection favors the transmission of bacteria and other infectious agents as well as the flow of genetic elements containing antibiotic resistance genes. This problem is worsened when pathogenic bacteria have the ability to establish as biofilms. Therefore, it is important to understand the characteristics and behaviour of microorganisms in both planktonic and biofilms states from the most diverse environmental niches to mitigate the emergence and dissemination of resistance.Methods. The purpose of this work was to assess the antibiotic susceptibility of four bacteria (Acinetobacter spp., Klebsiella pneumoniae, Pseudomonas fluorescens and Shewanella putrefaciens) isolated from Wild Animals and their ability to form biofilms. The effect of two antibiotics, imipenem (IPM) and ciprofloxacin (CIP), on biofilm removal was also assessed. Screening of resistance genetic determinants was performed by PCR. Biofilm tests were performed by a modified microtiter plate method. Bacterial surface hydrophobicity was determined by sessile drop contact angles.Results. The susceptibility profile classified the bacteria as multidrug-resistant. Three genes coding for beta-lactamases were detected in K. pneumoniae (TEM, SHV, OXA-aer) and one in P. fluorescens (OXA-aer). K. pneumoniae was the microorganism that carried more beta-lactamase genes and it was the most proficient biofilm producer, while P. fluorescens demonstrated the highest adhesion ability. Antibiotics at their MIC, 5 x MIC and 10 x MIC were ineffective in total biofilm removal. The highest biomass reductions were found with IPM (54% at 10 x MIC) against K. pneumoniae biofilms and with CIP (40% at 10 x MIC) against P. fluorescens biofilms.Discussion. The results highlight Wildlife as important host reservoirs and vectors for the spread of multidrug-resistant bacteria and genetic determinants of resistance. The ability of these bacteria to form biofilms should increase their persistence
Christine Strussmann - One of the best experts on this subject based on the ideXlab platform.
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rickettsial infection in ticks acari ixodidae of Wild Animals in midwestern brazil
Ticks and Tick-borne Diseases, 2016Co-Authors: Rute Witter, Sandra Helena Ramiro Corrêa, Thais Oliveira Morgado, Afonso Lodovico Sinkoc, Artur Kanadani Campos, Andreia Lima Tome Melo, R W Wolf, Joares Adenilson Mayjunior, Christine StrussmannAbstract:Abstract Ticks collected in the last two decades from free-living and captive Wild Animals from 28 municipalities of the Brazilian state of Mato Grosso were identified and tested using molecular methods for the presence of rickettsial agents. A total of 4467 ticks (229 larvae, 1676 nymphs, 1565 males, 997 females) representing 27 ixodid species were collected from 235 species of amphibians, reptiles, birds, and mammals from three different ecoregions (Pantanal, Cerrado, and Amazonia). The species Amblyomma parkeri , Amblyomma romitii , Amblyomma varium and Ixodes luciae are reported for the first time in the state of Mato Grosso. Amongst 538 ticks tested by molecular methods for rickettsial infection, we detected ‘ Candidatus Rickettsia amblyommii’ infecting Amblyomma cajennense sensu stricto and Amblyomma coelebs , Rickettsia sp. strain Atlantic rainforest infecting Amblyomma ovale , Rickettsia sp. strain NOD infecting Amblyomma nodosum , and ‘ Candidatus Rickettsia andeanae’ infecting Amblyomma sculptum . Our results represent an impressive expansion of knowledge on tick fauna and rickettsiae and are essential for understanding the ecology of ticks and tick-borne diseases in the Neotropical region, particularly in midwestern Brazil.
Eduardo Bagagli - One of the best experts on this subject based on the ideXlab platform.
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Molecular detection of Paracoccidioides brasiliensis in road-killed Wild Animals
Medical Mycology, 2008Co-Authors: Virgínia Bodelão Richini-pereira, Juliana Griese, Raquel Cordeiro Theodoro, Severino Assis Da Graça Macoris, Ligia Vizeu Barrozo, Patrícia Morais E Silva Tavares, Rosely Maria Zancopé-oliveira, Sandra De Moraes Gimenes Bosco, Reinaldo José Da Silva, Eduardo BagagliAbstract:Paracoccidioides brasiliensis infections have been little studied in Wild and/or domestic Animals, which may represent an important indicator of the presence of the pathogen in nature. Road-killed Wild Animals have been used for surveillance of vectors of zoonotic pathogens and may offer new opportunities for eco-epidemiological studies of paracoccidiodomycosis (PCM). The presence of P. brasiliensis infection was evaluated by Nested-PCR in tissue samples collected from 19 roadkilled Animals; 3 Cavia aperea (guinea pig), 5 Cerdocyon thous (crab-eating-fox), 1 Dasypus novemcinctus (nine-banded armadillo), 1 Dasypus septemcinctus (sevenbanded armadillo), 2 Didelphis albiventris (white-eared opossum), 1 Eira barbara (tayra), 2 Gallictis vittata (grison), 2 Procyon cancrivorus (raccoon) and 2 Sphiggurus spinosus (porcupine). Specific P. brasiliensis amplicons were detected in (a) several organs of the two armadillos and one guinea pig, (b) the lung and liver of the porcupine, and (c) the lungs of raccoons and grisons. P. brasiliensis infection in Wild Animals from endemic areas might be more common than initially postulated. Molecular techniques can be used for detecting new hosts and mapping ‘hot spot’ areas of PCM.
