The Experts below are selected from a list of 606 Experts worldwide ranked by ideXlab platform
Riedel H - One of the best experts on this subject based on the ideXlab platform.
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Epidermal growth factor (EGF) modulation of Feline Sarcoma Virus fms tyrosine kinase activity, internalization, degradation, and transforming potential in an EGF receptor/v-fms chimera.
1994Co-Authors: Riedel HAbstract:The Feline Sarcoma Virus oncogene v-fms has significantly contributed to the dissection of peptide growth factor action since it encodes the transmembrane tyrosine kinase gp140v-fms, a transforming version of colony-stimulating factor 1 receptor, a member of the growth factor receptor tyrosine kinase family. In this study, the functional significance of structural differences between distinct tyrosine kinase types, in particular between cellular receptors and viral transforming proteins of distinct structural types, has been further investigated, and their functional compatibility has been addressed. For this purpose, major functional domains of three structurally distinct tyrosine kinases were combined into two chimeric receptors. The cytoplasmic gp140v-fms kinase domain and the kinase domain of Rous Sarcoma Virus pp60v-src were each fused to the extracellular ligand-binding domain of the epidermal growth factor (EGF) receptor to create chimeras EFR and ESR, respectively, which were studied upon stable expression in NIH 3T3 fibroblasts. Both chimeras were faithfully synthesized and routed to the cell surface, where they displayed EGF-specific, low-affinity ligand-binding domains in contrast to the high- and low-affinity EGF-binding sites of normal EGF receptors. While the EFR kinase was EGF controlled for autophosphorylation and substrate phosphorylation in vitro, in vivo, and in digitonin-treated cells, the ESR kinase was not responsive to EGF. While ESR appeared to recycle to the cell surface upon endocytosis, EGF induced efficient EFR internalization and degradation, and phorbol esters stimulated protein kinase C-mediated downmodulation of EFR. Despite its ligand-inducible kinase activity, EFR was partly EGF independent in mediating mitogenesis and cell transformation, while ESR appeared biologically inactive
Bruce H. Grahn - One of the best experts on this subject based on the ideXlab platform.
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Lack of detection of Feline leukemia and Feline Sarcoma Viruses in diffuse iris melanomas of cats by immunohistochemistry and polymerase chain reaction.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians Inc, 2002Co-Authors: Cheryl L. Cullen, Deborah M. Haines, Marion L. Jackson, Bruce H. GrahnAbstract:Diffuse iris melanoma was confirmed by light-microscopic examination in 10 formalin-fixed, paraffin-embedded globes from 10 cats. To determine if Feline leukemia Virus or a replication defective Feline leukemia Virus, Feline Sarcoma Virus, was present in these anterior uveal melanomas, immunohistochemistry and polymerase chain reaction for Feline leukemia Virus were utilized. Immunohistochemical staining for Feline leukemia Virus glycoprotein 70 was performed on all 10 tumors using an avidin-biotin complex technique. The DNA was extracted from each specimen and a 166-base pair region of the Feline leukemia Virus long terminal repeat was targeted by polymerase chain reaction. Immunohistochemical staining for Feline leukemia Virus glycoprotein 70 and polymerase chain reaction amplification of a Feline leukemia Virus long terminal repeat region were negative in all cases. Feline leukemia Virus/Feline Sarcoma Virus was not detected in any neoplasms and therefore was unlikely to play a role in the tumorigenesis of these Feline diffuse iris melanomas.
David O - One of the best experts on this subject based on the ideXlab platform.
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Regression of Feline Sarcoma Virus-Induced Sarcomas in Dogs. II. Immunologic Investigations 1,2
2016Co-Authors: David OAbstract:activity, and Virus-neutralizing antibody levels were moni-tored in vitro for beagle and mongrel puppies inoculated with Feline Sarcoma Virus (FeSV) and were compared to in vivo histologic markers of regression of induced Sarcomas. CMI developed rapidly and maintained a high level of in vitro activity throughout the tumor life-span. Cytotoxic antibody levels similarly rose rapidly to peak just before clinically detectable regression and then declined during most of the regression sequence. This suggested antibody fixation at the tumor site, correlating with the histologic finding of focal necrosis and neutrophilic infiltrates. I nactivation of antibody by circulating antigen with sub-sequent immune complex formation was a possibility. Levels of Virus-neutralizing antibody in sera paralleled those of cytotoxic antibody; their relationship in the cir-culation was not clear, but each related to Virus-determined antigenic specificities. Serum blocking activity rose rapidly, leveled oft during most ot the tumor life-span, and rose slightly during the last stages of regression. This partly explained the lack of in vivo tumor lymphoid infil-trates to correlate with the striking in vitro CMI. Blocking activity was also present, however, when lymphoid in-filtrates were seen histologically. Thus in vitro-in vivo correlation was best for cytotoxic antibody, which sug-gested that antigen-antibody reactions involving neu-trophil-mediated regression sequences were important in etfecting tumor-cell destruction.-J Natl Cancer Ins
Anton Svitin - One of the best experts on this subject based on the ideXlab platform.
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DATA NOTE Open Access Annotated features of domestic cat – Felis catus genome
2016Co-Authors: Gaik Tamazian, Alexey I Makunin, Serguei Simonov, Pavel Dobrynin, Anton Logachev, Aleksey Komissarov, Andrey Shevchenko, Vladimir Brukhin, Nikolay Cherkasov, Anton SvitinAbstract:Background: Domestic cats enjoy an extensive veterinary medical surveillance which has described nearly 250 genetic diseases analogous to human disorders. Feline infectious agents offer powerful natural models of deadly human diseases, which include Feline immunodeficiency Virus, Feline Sarcoma Virus and Feline leukemia Virus. A rich veterinary literature of Feline disease pathogenesis and the demonstration of a highly conserved ancestral mammal genome organization make the cat genome annotation a highly informative resource that facilitates multifaceted research endeavors. Findings: Here we report a preliminary annotation of the whole genome sequence of Cinnamon, a domestic cat living in Columbia (MO, USA), bisulfite sequencing of Boris, a male cat from St. Petersburg (Russia), and light 30× sequencing of Sylvester, a European wildcat progenitor of cat domestication. The annotation includes 21,865 protein-coding genes identified by a comparative approach, 217 loci of endogenous retroVirus-like elements, repetitive elements which comprise about 55.7 % of the whole genome, 99,494 new SNVs, 8,355 new indels, 743,326 evolutionary constrained elements, and 3,182 microRNA homologues. The methylation sites study shows that 10.5% of cat genome cytosines are methylated. An assisted assembly of a European wildcat, Felis silvestris silvestris, was performed; variants between F. silvestris and F. catus genomes were derived and compared to F. catus
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annotated features of domestic cat felis catus genome
GigaScience, 2014Co-Authors: Gaik Tamazian, Alexey I Makunin, Serguei Simonov, Pavel Dobrynin, Anton Logachev, Aleksey Komissarov, Andrey Shevchenko, Vladimir Brukhin, Nikolay Cherkasov, Anton SvitinAbstract:Domestic cats enjoy an extensive veterinary medical surveillance which has described nearly 250 genetic diseases analogous to human disorders. Feline infectious agents offer powerful natural models of deadly human diseases, which include Feline immunodeficiency Virus, Feline Sarcoma Virus and Feline leukemia Virus. A rich veterinary literature of Feline disease pathogenesis and the demonstration of a highly conserved ancestral mammal genome organization make the cat genome annotation a highly informative resource that facilitates multifaceted research endeavors. Here we report a preliminary annotation of the whole genome sequence of Cinnamon, a domestic cat living in Columbia (MO, USA), bisulfite sequencing of Boris, a male cat from St. Petersburg (Russia), and light 30× sequencing of Sylvester, a European wildcat progenitor of cat domestication. The annotation includes 21,865 protein-coding genes identified by a comparative approach, 217 loci of endogenous retroVirus-like elements, repetitive elements which comprise about 55.7% of the whole genome, 99,494 new SNVs, 8,355 new indels, 743,326 evolutionary constrained elements, and 3,182 microRNA homologues. The methylation sites study shows that 10.5% of cat genome cytosines are methylated. An assisted assembly of a European wildcat, Felis silvestris silvestris, was performed; variants between F. silvestris and F. catus genomes were derived and compared to F. catus. The presented genome annotation extends beyond earlier ones by closing gaps of sequence that were unavoidable with previous low-coverage shotgun genome sequencing. The assembly and its annotation offer an important resource for connecting the rich veterinary and natural history of cats to genome discovery.
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Annotated features of domestic cat – Felis catus genome
GigaScience, 2014Co-Authors: Gaik Tamazian, Alexey I Makunin, Serguei Simonov, Pavel Dobrynin, Anton Logachev, Aleksey Komissarov, Andrey Shevchenko, Vladimir Brukhin, Nikolay Cherkasov, Anton SvitinAbstract:Background Domestic cats enjoy an extensive veterinary medical surveillance which has described nearly 250 genetic diseases analogous to human disorders. Feline infectious agents offer powerful natural models of deadly human diseases, which include Feline immunodeficiency Virus, Feline Sarcoma Virus and Feline leukemia Virus. A rich veterinary literature of Feline disease pathogenesis and the demonstration of a highly conserved ancestral mammal genome organization make the cat genome annotation a highly informative resource that facilitates multifaceted research endeavors. Findings Here we report a preliminary annotation of the whole genome sequence of Cinnamon, a domestic cat living in Columbia (MO, USA), bisulfite sequencing of Boris, a male cat from St. Petersburg (Russia), and light 30× sequencing of Sylvester, a European wildcat progenitor of cat domestication. The annotation includes 21,865 protein-coding genes identified by a comparative approach, 217 loci of endogenous retroVirus-like elements, repetitive elements which comprise about 55.7 % of the whole genome, 99,494 new SNVs, 8,355 new indels, 743,326 evolutionary constrained elements, and 3,182 microRNA homologues. The methylation sites study shows that 10.5 % of cat genome cytosines are methylated. An assisted assembly of a European wildcat, Felis silvestris silvestris , was performed; variants between F. silvestris and F. catus genomes were derived and compared to F. catus . Conclusions The presented genome annotation extends beyond earlier ones by closing gaps of sequence that were unavoidable with previous low-coverage shotgun genome sequencing. The assembly and its annotation offer an important resource for connecting the rich veterinary and natural history of cats to genome discovery.
Donald L. Dungworth - One of the best experts on this subject based on the ideXlab platform.
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Regression of Feline Sarcoma Virus-Induced Sarcomas in Dogs. I. Morphologic Investigations 1,2
2016Co-Authors: David O. Slauson, B. I. Osburn, Moshe Shifrine, Donald L. DungworthAbstract:SUMMARY-lIT a study of morphologic changes in the development and regression ot Feline Sarcoma Virus (FeSV)-induced tumors in dogs, 27 weaned and newborn beagle and mongrel puppies were inoculated with FeSV in doses from 1.0 to 3.0 gEq; 2 beagle and 2 mongrel puppies were used as uninoculated contact controls. All animals were examined daily, and crude tumor volume was calculated from length, width, and depth measurements of the neo-plasms. Biopsies were done at various stages of tumor development and regression. When tumors were no longer palpable, all puppies were necropsied. Two of 8 (25%) weaned beagle puppies, 10 of 12 (83%) newborn beagles, and 1 of 7 (14%) newborn mongrels developed tumors, all histologically confirmed fibroSarcomas. No metastatic tumor foci were detected. The tumor life-span was divided into approximately equal periods ot 9rowth and regression. The initial regression period was characterized by focal necrosis and accompanying neutrophil intiltration. The later stages of regression were characterized by lympho-cytic or mixed mononuclear infiltrates. Thus the regression histopathology was not uniform and suggested that dif-ferent immunologic mediation systems effect re9ression. Nonneoplastic morphologic changes consisted largely of lymphoid depletion and necrosis in lymph nodes an
