The Experts below are selected from a list of 57 Experts worldwide ranked by ideXlab platform
Michael R Lappin - One of the best experts on this subject based on the ideXlab platform.
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Feline panleukopenia virus Feline herpesvirus 1 and Feline calicivirus antibody responses in seronegative specific pathogen free kittens after parenteral administration of an inactivated fvrcp vaccine or a modified live fvrcp vaccine
Journal of Feline Medicine and Surgery, 2012Co-Authors: Michael R LappinAbstract:Two groups of Feline panleukopenia (FPV), Feline calicivirus (FCV) and Feline herpesvirus 1 (FHV-1) seronegative kittens (six cats per group) were administered one of two Feline Viral Rhinotracheitis, calcivirus and panleukopenia (FVRCP) vaccines subcutaneously (one inactivated and one modified live) and the serological responses to each agent were followed over 49 days (days 0, 2, 5, 7, 10, 14, 21, 28, 35, 42, 49). While the kittens administered the modified live FPV vaccine were more likely to seroconvert on day 7 after the first inoculation than kittens administered the inactivated vaccine, all kittens had seroconverted by day 14. In contrast, FHV-1 serological responses were more rapid following administration of the inactivated FVRCP vaccine when compared with the modified live FVRCP vaccine. There were no statistical differences between the serological response rates between the two FVRCP vaccines in regard to FCV.
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Feline panleukopenia virus Feline herpesvirus 1 and Feline calicivirus antibody responses in seronegative specific pathogen free cats after a single administration of two different modified live fvrcp vaccines
Journal of Feline Medicine and Surgery, 2009Co-Authors: Michael R Lappin, Julia K Veir, Jennifer R HawleyAbstract:Two groups of Feline panleukopenia virus (FPV), Feline calicivirus (FCV), and Feline herpesvirus-1 (FHV-1) seronegative cats (five cats per group) were administered one of two modified live Feline Viral Rhinotracheitis, calicivirus, and panleukopenia virus (FVRCP) vaccines and the serological responses to each agent were followed over 28 days. While all cats developed detectable FPV and FCV antibody titers; only two cats developed detectable FHV-1 antibody titers using the criteria described by the testing laboratory. For FPV and FHV-1, there were no differences in seroconversion rates between the cats that were administered the intranasal (IN) FVRCP vaccine and the cats that were administered the parenteral FVRCP vaccine on any day post-inoculation. For FCV, the cats that were administered the IN FVRCP vaccine were more likely to seroconvert on days 10 and 14 when compared to cats that were administered the parenteral FVRCP vaccine.
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investigation of the induction of antibodies against crandell rees Feline kidney cell lysates and Feline renal cell lysates after parenteral administration of vaccines against Feline Viral Rhinotracheitis calicivirus and panleukopenia in cats
American Journal of Veterinary Research, 2005Co-Authors: Michael R Lappin, Wayne A Jensen, Tracey Jensen, Randall J Basaraba, Cathy A Brown, Steven V Radecki, Jennifer R HawleyAbstract:Objective—To determine whether administration of Crandell-Rees Feline kidney (CRFK) cell lysates or vaccines against Feline Viral Rhinotracheitis, calicivirus, and panleukopenia (FVRCP vaccines) that likely contain CRFK cell proteins induces antibodies against CRFK cell or Feline renal cell (FRC) lysates in cats. Animals—14 eight-week-old cats. Procedure—Before and after the study, renal biopsy specimens were obtained from each cat for histologic evaluation. Each of 4 FVRCP vaccines was administered to 2 cats at weeks 0, 3, 6, and 50. Between weeks 0 and 50, another 3 pairs of cats received 11 CRFK cell lysate inoculations SC (10, 50, or 50 µg mixed with alum). Clinicopathologic evaluations and ELISAs to detect serum antibodies against CRFK cell or FRC lysates were performed at intervals. Results—Cats had no antibodies against CRFK cell or FRC lysates initially. All cats administered CRFK cell lysate had detectable antibodies against CRFK cell or FRC lysates on multiple occasions. Of 6 cats vaccinated par...
Jennifer R Hawley - One of the best experts on this subject based on the ideXlab platform.
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Feline panleukopenia virus Feline herpesvirus 1 and Feline calicivirus antibody responses in seronegative specific pathogen free cats after a single administration of two different modified live fvrcp vaccines
Journal of Feline Medicine and Surgery, 2009Co-Authors: Michael R Lappin, Julia K Veir, Jennifer R HawleyAbstract:Two groups of Feline panleukopenia virus (FPV), Feline calicivirus (FCV), and Feline herpesvirus-1 (FHV-1) seronegative cats (five cats per group) were administered one of two modified live Feline Viral Rhinotracheitis, calicivirus, and panleukopenia virus (FVRCP) vaccines and the serological responses to each agent were followed over 28 days. While all cats developed detectable FPV and FCV antibody titers; only two cats developed detectable FHV-1 antibody titers using the criteria described by the testing laboratory. For FPV and FHV-1, there were no differences in seroconversion rates between the cats that were administered the intranasal (IN) FVRCP vaccine and the cats that were administered the parenteral FVRCP vaccine on any day post-inoculation. For FCV, the cats that were administered the IN FVRCP vaccine were more likely to seroconvert on days 10 and 14 when compared to cats that were administered the parenteral FVRCP vaccine.
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investigation of the induction of antibodies against crandell rees Feline kidney cell lysates and Feline renal cell lysates after parenteral administration of vaccines against Feline Viral Rhinotracheitis calicivirus and panleukopenia in cats
American Journal of Veterinary Research, 2005Co-Authors: Michael R Lappin, Wayne A Jensen, Tracey Jensen, Randall J Basaraba, Cathy A Brown, Steven V Radecki, Jennifer R HawleyAbstract:Objective—To determine whether administration of Crandell-Rees Feline kidney (CRFK) cell lysates or vaccines against Feline Viral Rhinotracheitis, calicivirus, and panleukopenia (FVRCP vaccines) that likely contain CRFK cell proteins induces antibodies against CRFK cell or Feline renal cell (FRC) lysates in cats. Animals—14 eight-week-old cats. Procedure—Before and after the study, renal biopsy specimens were obtained from each cat for histologic evaluation. Each of 4 FVRCP vaccines was administered to 2 cats at weeks 0, 3, 6, and 50. Between weeks 0 and 50, another 3 pairs of cats received 11 CRFK cell lysate inoculations SC (10, 50, or 50 µg mixed with alum). Clinicopathologic evaluations and ELISAs to detect serum antibodies against CRFK cell or FRC lysates were performed at intervals. Results—Cats had no antibodies against CRFK cell or FRC lysates initially. All cats administered CRFK cell lysate had detectable antibodies against CRFK cell or FRC lysates on multiple occasions. Of 6 cats vaccinated par...
Lloret A - One of the best experts on this subject based on the ideXlab platform.
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Feline herpesvirus infections ABCD guidelines on prevention and management
'Elsevier BV', 2009Co-Authors: Thiry E, Addie D, Belak S, Boucrat-baralon C, Egberink H, Frymus T, Gruffyd-jones T, Hartmann K, Hosie M.j, Lloret AAbstract:Feline Viral Rhinotracheitis, caused by Feline herpesvirus (FHV), is an upper respiratory tract disease that is often associated with Feline calicivirus and bacteria. In most cats, FHV remains latent after recovery, and they become lifelong virus carriers. Stress or corticosteroid treatment may lead to virus reactivation and shedding in oronasal and conjunctival secretions. Infection Sick cats shed FHV in oral, nasal and conjunctival secretions; shedding may last for 3 weeks. Infection requires direct contact with a shedding cat. Disease signs Feline herpesvirus infections cause acute rhinitis and conjunctivitis, usually accompanied by fever, depression and anorexia. Affected cats may also develop typical ulcerative, dendritic keratitis. Diagnosis Samples consist of conjunctival, corneal or oropharyngeal swabs, corneal scrapings or biopsies. It is not recommended that cats recently vaccinated with a modified-live virus vaccine are sampled. Positive PCR results should be interpreted with caution, as they may be produced by low-level shedding or Viral latency. Disease management 'Tender loving care' from the owner, supportive therapy and good nursing are essential. Anorexic cats should be fed blended, highly palatable food - warmed up if required. Mucolytic drugs (eg, bromhexine) or nebulisation with saline may offer relief. Broad-spectrum antibiotics should be given to prevent secondary bacterial infections. Topical antiViral drugs may be used for the treatment of acute FHV ocular disease. The virus is labile and susceptible to most disinfectants, antiseptics and detergents
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Feline herpesvirus infection. ABCD guidelines on prevention and management
Elsevier, 2009Co-Authors: Thiry E, Belak S, Egberink H, Frymus T, Hartmann K, Addie Diane, Boucraut-baralon C, Gruffydd-jones T, Hosie M J, Lloret AAbstract:OVERVIEW: Feline Viral Rhinotracheitis, caused by Feline herpesvirus (FHV), is an upper respiratory tract disease that is often associated with Feline calicivirus and bacteria. In most cats, FHV remains latent after recovery, and they become lifelong virus carriers. Stress or corticosteroid treatment may lead to virus reactivation and shedding in oronasal and conjunctival secretions. INFECTION: Sick cats shed FHV in oral, nasal and conjunctival secretions; shedding may last for 3 weeks. Infection requires direct contact with a shedding cat. DISEASE SIGNS: Feline herpesvirus infections cause acute rhinitis and conjunctivitis, usually accompanied by fever, depression and anorexia. Affected cats may also develop typical ulcerative, dendritic keratitis. DIAGNOSIS: Samples consist of conjunctival, corneal or oropharyngeal swabs, corneal scrapings or biopsies. It is not recommended that cats recently vaccinated with a modified-live virus vaccine are sampled. Positive PCR results should be interpreted with caution, as they may be produced by low-level shedding or Viral latency. DISEASE MANAGEMENT: 'Tender loving care' from the owner, supportive therapy and good nursing are essential. Anorexic cats should be fed blended, highly palatable food - warmed up if required. Mucolytic drugs (eg, bromhexine) or nebulisation with saline may offer relief. Broad-spectrum antibiotics should be given to prevent secondary bacterial infections. Topical antiViral drugs may be used for the treatment of acute FHV ocular disease. The virus is labile and susceptible to most disinfectants, antiseptics and detergents. VACCINATION RECOMMENDATIONS: Two injections, at 9 and 12 weeks of age, are recommended, with a first booster 1 year later. Boosters should be given annually to at-risk cats. For cats in low-risk situations (eg, indoor-only cats), 3-yearly intervals suffice. Cats that have recovered from FHV-associated disease are usually not protected for life against further disease episodes; vaccination of recovered cats is therefore recommended
E. I. Elizbarashvili - One of the best experts on this subject based on the ideXlab platform.
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COMPARATIVE STUDIES OF Feline Viral Rhinotracheitis VIRUS FOR ITS REPLICATION PROPERTIES IN DIFFERENT CELL CULTURES
'FGI Federal Centre for Animal Health (FGI ARRIA)', 2018Co-Authors: Ye. G. Kokorina, E. I. ElizbarashviliAbstract:The results of comparative studies of Feline Viral Rhinotracheitis virus for its culture properties in primary and continuous cell cultures of Feline origin (FK, FK (subculture), CrFK, FS, CC-81, FC/Tg) are presented. It was found that Viral Rhinotracheitis virus replication, irrespective of the route of infection and the culture technique, was consistent and practically equal in susceptible cell cultures. The most pronounced cytopathic effect (more than 75% monolayer degeneration) was observed in all types of cell cultures in 48–72 hours of cultivation. However, the accumulation of Feline Viral Rhinotracheitis virus Grand strain was highest when preliminary adsorption occurred within the specified period of time, monolayer cell cultures were infected with the virus at a dose of 5.5 lg TCID50/ml and roller bottle cultivated, and the рН of the medium was maintained at 7.0–7.4. Single freezing of the virus at a temperature of minus 60 degrees Celsius upon the completion of the cultivation cycle (during 60–72 hours) and its thawing were found to significantly increase the virus titre by 0.5 lg TCID50/ml
Roger K Maes - One of the best experts on this subject based on the ideXlab platform.
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felid herpesvirus type 1 infection in cats a natural host model for alphaherpesvirus pathogenesis
International Scholarly Research Notices, 2012Co-Authors: Roger K MaesAbstract:Feline herpesvirus 1 (FeHV-1) is an alphaherpesvirus that causes Feline Viral Rhinotracheitis, an important Viral disease of cats on a worldwide basis. Acute FeHV-1 infection is associated with both upper respiratory and ocular signs. Following the acute phase of the disease lifelong latency is established, primarily in sensory neuronal cells. As is the case with human herpes simplex viruses, latency reactivation can result in recrudescence, which can manifest itself in the form of serious ocular lesions. FeHV-1 infection in cats is a natural host model that is useful for the identification of Viral virulence genes that play a role in replication at the mucosal portals of entry or are mediators of the establishment, maintenance, or reactivation of latency. It is also a model system for defining innate and adaptive immunity mechanisms and for immunization strategies that can lead to better protection against this and other alphaherpesvirus infections.
