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Han Bok Kim - One of the best experts on this subject based on the ideXlab platform.

  • Inflammation-related signaling pathways implicating TGFβ are revealed in the expression profiling of MCF7 cell treated with Fermented Soybean, chungkookjang
    Nutrition and cancer, 2011
    Co-Authors: Jae Sung Hwang, Hyung Jae Yoo, Hye Jung Song, Kyukwang Kim, Young Jin Chun, Toshiro Matsui, Han Bok Kim
    Abstract:

    Chungkookjang is a Korean Fermented Soybean containing microorganisms, proteinase, and diverse bioactive compounds, including a high concentration of isoflavones and peptides. Growth of breast cancer MCF7 cells decreased dependent on the concentration of Fermented Soybean extracts. The effect of Fermented Soybean on cellular gene expression was determined in a systematic manner comprehensively. DNA microarray analysis was performed using 25,804 probes. Ninety one genes whose expression levels were significantly changed were selected. TGFβI and Smad3 were upregulated. Downregulation of inflammation-related CSF2, CSF2RA, and CSF3 was found. Differential expression of chemokines CCL2, CCL3, CCL3L3, CXCL1, and CXCL2 were observed. Network analysis identified ERβ in the network. Based on the experimental results, taking Fermented Soybean might be helpful for preventing breast cancer by a mechanism activating TGFβ pathway and depressing inflammation.

  • ethanol extract of Fermented Soybean chungkookjang inhibits the apoptosis of mouse spleen and thymus cells
    Journal of Microbiology, 2007
    Co-Authors: Han Bok Kim, Hye Sung Lee, Sook Jin Kim, Hyung Jae Yoo, Jae Sung Hwang, Gang Chen, Hyun Joo Youn
    Abstract:

    Apoptosis is a step of the cell cycle which is important in the regulation of immune cell populations. Chungkookjang is a Korean traditional Fermented Soybean containing microorganisms, enzymes, and bioactive compounds which was used in the treatment of mouse spleen as well as thymus cells (CH1-Fermented Soybean containing barley, wormwood, and sea tangle; CH2-Fermented Soybean) and was found to exhibit substantially reduced small DNA fragmentation. An MTT assay showed that the treatment of CH1 and CH2 into the mouse splenocytes and thymocytes sharply increased their survival. Moreover, a FACS analysis also showed that CH1 and CH2 are effective at suppressing the apoptosis of splenocytes and thymocytes. The Fermented Soybean isoflavone concentrations, which are implicated in lowering breast and prostate cancers, lowering the risk of cardiovascular diseases, and improving bone health, were determined using Capillary Electrophoresis-Electrochemical Detection (CE-ED). The amount of Daidzein in Fermented Soybean significantly increased by 44-fold dramatically, compared with those in unFermented Soybean. In this study, we demonstrated that ethanol extracts of Chungkookjang promote the survival of the mouse spleen and thymus cells in culture by suppressing their apoptotic death. Future studies should investigate which genes are related to apoptosis of the immune cells.

Jong Ho Lee - One of the best experts on this subject based on the ideXlab platform.

Jae Sung Hwang - One of the best experts on this subject based on the ideXlab platform.

  • Inflammation-related signaling pathways implicating TGFβ are revealed in the expression profiling of MCF7 cell treated with Fermented Soybean, chungkookjang
    Nutrition and cancer, 2011
    Co-Authors: Jae Sung Hwang, Hyung Jae Yoo, Hye Jung Song, Kyukwang Kim, Young Jin Chun, Toshiro Matsui, Han Bok Kim
    Abstract:

    Chungkookjang is a Korean Fermented Soybean containing microorganisms, proteinase, and diverse bioactive compounds, including a high concentration of isoflavones and peptides. Growth of breast cancer MCF7 cells decreased dependent on the concentration of Fermented Soybean extracts. The effect of Fermented Soybean on cellular gene expression was determined in a systematic manner comprehensively. DNA microarray analysis was performed using 25,804 probes. Ninety one genes whose expression levels were significantly changed were selected. TGFβI and Smad3 were upregulated. Downregulation of inflammation-related CSF2, CSF2RA, and CSF3 was found. Differential expression of chemokines CCL2, CCL3, CCL3L3, CXCL1, and CXCL2 were observed. Network analysis identified ERβ in the network. Based on the experimental results, taking Fermented Soybean might be helpful for preventing breast cancer by a mechanism activating TGFβ pathway and depressing inflammation.

  • ethanol extract of Fermented Soybean chungkookjang inhibits the apoptosis of mouse spleen and thymus cells
    Journal of Microbiology, 2007
    Co-Authors: Han Bok Kim, Hye Sung Lee, Sook Jin Kim, Hyung Jae Yoo, Jae Sung Hwang, Gang Chen, Hyun Joo Youn
    Abstract:

    Apoptosis is a step of the cell cycle which is important in the regulation of immune cell populations. Chungkookjang is a Korean traditional Fermented Soybean containing microorganisms, enzymes, and bioactive compounds which was used in the treatment of mouse spleen as well as thymus cells (CH1-Fermented Soybean containing barley, wormwood, and sea tangle; CH2-Fermented Soybean) and was found to exhibit substantially reduced small DNA fragmentation. An MTT assay showed that the treatment of CH1 and CH2 into the mouse splenocytes and thymocytes sharply increased their survival. Moreover, a FACS analysis also showed that CH1 and CH2 are effective at suppressing the apoptosis of splenocytes and thymocytes. The Fermented Soybean isoflavone concentrations, which are implicated in lowering breast and prostate cancers, lowering the risk of cardiovascular diseases, and improving bone health, were determined using Capillary Electrophoresis-Electrochemical Detection (CE-ED). The amount of Daidzein in Fermented Soybean significantly increased by 44-fold dramatically, compared with those in unFermented Soybean. In this study, we demonstrated that ethanol extracts of Chungkookjang promote the survival of the mouse spleen and thymus cells in culture by suppressing their apoptotic death. Future studies should investigate which genes are related to apoptosis of the immune cells.

Man Jinhao - One of the best experts on this subject based on the ideXlab platform.

  • Analysis of the solid-state Fermented Soybean meal by Monascus.
    Modern Food Science and Technology, 2009
    Co-Authors: Liu Anjun, Shang Xiaolan, Zhu Zhenyuan, Man Jinhao
    Abstract:

    M. rubber(Monascus purpureus) mediated solid state fermentation of the Soybean meal was studied. The high-value Fermented Soybean meal was obtained with the ratio of Soybean meal to water being of 1:1. The color-value,the comparison of the protein type,the contents of protein,and the detection of polypeptide before the fermentation and after the fermentation was determined and the contents of Monacolin K in the Fermented product were determined. The results showed that the color-value of the solid-state Fermented Soybean mea was low and the solubility of the protein in the Fermented Soybean was reduced due to denaturation by steam heating. Through the solid-state fermentation,the contents of the soluble protein increased and the proteins decomposed to peptides and thus improve the function of the protein. Low content of Monacolin K was found in the Fermented Soybean meal,which indicated that the solid state fermentation of Soybean meal by Monascus was unsuitable to the production of Monascus Pigment and Monacolin K.

Seong-il Lim - One of the best experts on this subject based on the ideXlab platform.