The Experts below are selected from a list of 300 Experts worldwide ranked by ideXlab platform

Haiyan Xu - One of the best experts on this subject based on the ideXlab platform.

  • Comparative study of in vitro effects of different nanoparticles at non-cytotoxic concentration on the adherens junction of human vascular endothelial cells
    International Journal of Nanomedicine, 2019
    Co-Authors: Aiyun Yang, Lifan Du, Lingyu Piao, Jie Meng, Haiyan Xu
    Abstract:

    Effects of different nanoparticles (NPs) exposure at acutely non-cytotoxic concentrations are particularly worthy to figure out, compare, and elucidate. To investigate and compare the effect of a small library of NPs at non-cytotoxic concentration on the adherens junction of human umbilical vein endothelial cells (HUVECs), obtaining new insights of NPs safety evaluation. The HUVECs layer was exposed to NPs including gold (Au), platinum (Pt), silica (SiO2), titanium diOxide (TiO2), Ferric Oxide (fe2o3), oxidized multi-walled carbon nanotubes, with different surface chemistry and size distribution. Cellular uptake of NPs was observed by transmission electron microscopy. and the cytotoxicity was determined by Cell Counting Kit-8 assay. The NP-induced variation of intracellular reactive oxygen species (ROS) and catalase (CAT) activity was measured using the probe of 2'7'-dichlorodihydr fluorescein diacetate and a CAT analysis kit, respectively. The level of VE-cadherin of HUVECs was analyzed by Western blot, and the loss of adherens junction was observed with laser confocal microscopy. The acutely non-cytotoxic concentrations of different NPs were determined and applied to HUVECs. The NPs increased the level of intracellular ROS and the activity of CAT to different degrees, depending on the characteristics. At the same time, the HUVECs lost their adherens junction protein VE-cadherin and gaps were formed between the cells. The NP-induced oxidative stress and gap formation could be rescued by the supplementary N-acetylcysteine in the incubation. The increase of intracellular ROS and CAT activity was one common effect of NPs, even at the non-cytotoxic concentration, and the degree was dependent on the composition, surface chemistry, and size distribution of the NP. The effect led to the gap formation between the cells, while could be rescued by the antioxidant. Therefore, the variation of adherens junction between endothelial cells was suggested to evaluate for NPs when used as therapeutics and diagnostics.

  • comparative study of in vitro effects of different nanoparticles at non cytotoxic concentration on the adherens junction of human vascular endothelial cells
    International Journal of Nanomedicine, 2019
    Co-Authors: Aiyun Yang, Lifan Du, Lingyu Piao, Jie Meng, Haiyan Xu
    Abstract:

    Background: Effects of different nanoparticles (NPs) exposure at acutely non-cytotoxic concentrations are particularly worthy to figure out, compare, and elucidate. Objective: To investigate and compare the effect of a small library of NPs at non-cytotoxic concentration on the adherens junction of human umbilical vein endothelial cells (HUVECs), obtaining new insights of NPs safety evaluation. Materials and methods: The HUVECs layer was exposed to NPs including gold (Au), platinum (Pt), silica (SiO2), titanium diOxide (TiO2), Ferric Oxide (fe2o3), oxidized multi-walled carbon nanotubes, with different surface chemistry and size distribution. Cellular uptake of NPs was observed by transmission electron microscopy. and the cytotoxicity was determined by Cell Counting Kit-8 assay. The NP-induced variation of intracellular reactive oxygen species (ROS) and catalase (CAT) activity was measured using the probe of 2'7'-dichlorodihydr fluorescein diacetate and a CAT analysis kit, respectively. The level of VE-cadherin of HUVECs was analyzed by Western blot, and the loss of adherens junction was observed with laser confocal microscopy. Results: The acutely non-cytotoxic concentrations of different NPs were determined and applied to HUVECs. The NPs increased the level of intracellular ROS and the activity of CAT to different degrees, depending on the characteristics. At the same time, the HUVECs lost their adherens junction protein VE-cadherin and gaps were formed between the cells. The NP-induced oxidative stress and gap formation could be rescued by the supplementary N-acetylcysteine in the incubation. Conclusion: The increase of intracellular ROS and CAT activity was one common effect of NPs, even at the non-cytotoxic concentration, and the degree was dependent on the composition, surface chemistry, and size distribution of the NP. The effect led to the gap formation between the cells, while could be rescued by the antioxidant. Therefore, the variation of adherens junction between endothelial cells was suggested to evaluate for NPs when used as therapeutics and diagnostics.

Qingyu Li - One of the best experts on this subject based on the ideXlab platform.

  • core shell structure carbon coated Ferric Oxide fe2o3 c nanoparticles for supercapacitors with superior electrochemical performance
    Journal of Alloys and Compounds, 2015
    Co-Authors: Yipeng Ye, Haiyan Zhang, Yiming Chen, Peng Deng, Zhikun Huang, Yannan Qian, Yunyong Li, Qingyu Li
    Abstract:

    Abstract Core–shell structure carbon coated Ferric Oxide nanoparticles (Fe 2 O 3 @C) were fabricated by the oxidation of carbon coated iron nanoparticles (Fe@C) prepared by a direct current carbon arc discharge method. Porous activated-Fe 2 O 3 @C was prepared by KOH activation of Fe 2 O 3 @C at the temperature of 750 °C. X-ray diffraction analysis (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were employed to characterize the structure and morphology of the Fe 2 O 3 @C and activated-Fe 2 O 3 @C. The specific surface area and pore size distribution of the samples were also tested. The activated-Fe 2 O 3 @C electrodes exhibited good electrochemical performance with a maximum specific capacitance of 612 F g −1 at the charge/discharge current density of 0.5 A g −1 with 5 M NaOH electrolyte. After 10,000 cycling DC tests at the charge/discharge current density of 4 A g −1 , a high level specific capacitance of 518 F g −1 was obtained (90.6% retention of the initial capacity), suggesting excellent long-term cycling stability.

  • Core–shell structure carbon coated Ferric Oxide (fe2o3@C) nanoparticles for supercapacitors with superior electrochemical performance
    Journal of Alloys and Compounds, 2015
    Co-Authors: Yipeng Ye, Haiyan Zhang, Yiming Chen, Peng Deng, Zhikun Huang, Yannan Qian, Yunyong Li, Qingyu Li
    Abstract:

    Abstract Core–shell structure carbon coated Ferric Oxide nanoparticles (Fe 2 O 3 @C) were fabricated by the oxidation of carbon coated iron nanoparticles (Fe@C) prepared by a direct current carbon arc discharge method. Porous activated-Fe 2 O 3 @C was prepared by KOH activation of Fe 2 O 3 @C at the temperature of 750 °C. X-ray diffraction analysis (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were employed to characterize the structure and morphology of the Fe 2 O 3 @C and activated-Fe 2 O 3 @C. The specific surface area and pore size distribution of the samples were also tested. The activated-Fe 2 O 3 @C electrodes exhibited good electrochemical performance with a maximum specific capacitance of 612 F g −1 at the charge/discharge current density of 0.5 A g −1 with 5 M NaOH electrolyte. After 10,000 cycling DC tests at the charge/discharge current density of 4 A g −1 , a high level specific capacitance of 518 F g −1 was obtained (90.6% retention of the initial capacity), suggesting excellent long-term cycling stability.

Aiyun Yang - One of the best experts on this subject based on the ideXlab platform.

  • Comparative study of in vitro effects of different nanoparticles at non-cytotoxic concentration on the adherens junction of human vascular endothelial cells
    International Journal of Nanomedicine, 2019
    Co-Authors: Aiyun Yang, Lifan Du, Lingyu Piao, Jie Meng, Haiyan Xu
    Abstract:

    Effects of different nanoparticles (NPs) exposure at acutely non-cytotoxic concentrations are particularly worthy to figure out, compare, and elucidate. To investigate and compare the effect of a small library of NPs at non-cytotoxic concentration on the adherens junction of human umbilical vein endothelial cells (HUVECs), obtaining new insights of NPs safety evaluation. The HUVECs layer was exposed to NPs including gold (Au), platinum (Pt), silica (SiO2), titanium diOxide (TiO2), Ferric Oxide (fe2o3), oxidized multi-walled carbon nanotubes, with different surface chemistry and size distribution. Cellular uptake of NPs was observed by transmission electron microscopy. and the cytotoxicity was determined by Cell Counting Kit-8 assay. The NP-induced variation of intracellular reactive oxygen species (ROS) and catalase (CAT) activity was measured using the probe of 2'7'-dichlorodihydr fluorescein diacetate and a CAT analysis kit, respectively. The level of VE-cadherin of HUVECs was analyzed by Western blot, and the loss of adherens junction was observed with laser confocal microscopy. The acutely non-cytotoxic concentrations of different NPs were determined and applied to HUVECs. The NPs increased the level of intracellular ROS and the activity of CAT to different degrees, depending on the characteristics. At the same time, the HUVECs lost their adherens junction protein VE-cadherin and gaps were formed between the cells. The NP-induced oxidative stress and gap formation could be rescued by the supplementary N-acetylcysteine in the incubation. The increase of intracellular ROS and CAT activity was one common effect of NPs, even at the non-cytotoxic concentration, and the degree was dependent on the composition, surface chemistry, and size distribution of the NP. The effect led to the gap formation between the cells, while could be rescued by the antioxidant. Therefore, the variation of adherens junction between endothelial cells was suggested to evaluate for NPs when used as therapeutics and diagnostics.

  • comparative study of in vitro effects of different nanoparticles at non cytotoxic concentration on the adherens junction of human vascular endothelial cells
    International Journal of Nanomedicine, 2019
    Co-Authors: Aiyun Yang, Lifan Du, Lingyu Piao, Jie Meng, Haiyan Xu
    Abstract:

    Background: Effects of different nanoparticles (NPs) exposure at acutely non-cytotoxic concentrations are particularly worthy to figure out, compare, and elucidate. Objective: To investigate and compare the effect of a small library of NPs at non-cytotoxic concentration on the adherens junction of human umbilical vein endothelial cells (HUVECs), obtaining new insights of NPs safety evaluation. Materials and methods: The HUVECs layer was exposed to NPs including gold (Au), platinum (Pt), silica (SiO2), titanium diOxide (TiO2), Ferric Oxide (fe2o3), oxidized multi-walled carbon nanotubes, with different surface chemistry and size distribution. Cellular uptake of NPs was observed by transmission electron microscopy. and the cytotoxicity was determined by Cell Counting Kit-8 assay. The NP-induced variation of intracellular reactive oxygen species (ROS) and catalase (CAT) activity was measured using the probe of 2'7'-dichlorodihydr fluorescein diacetate and a CAT analysis kit, respectively. The level of VE-cadherin of HUVECs was analyzed by Western blot, and the loss of adherens junction was observed with laser confocal microscopy. Results: The acutely non-cytotoxic concentrations of different NPs were determined and applied to HUVECs. The NPs increased the level of intracellular ROS and the activity of CAT to different degrees, depending on the characteristics. At the same time, the HUVECs lost their adherens junction protein VE-cadherin and gaps were formed between the cells. The NP-induced oxidative stress and gap formation could be rescued by the supplementary N-acetylcysteine in the incubation. Conclusion: The increase of intracellular ROS and CAT activity was one common effect of NPs, even at the non-cytotoxic concentration, and the degree was dependent on the composition, surface chemistry, and size distribution of the NP. The effect led to the gap formation between the cells, while could be rescued by the antioxidant. Therefore, the variation of adherens junction between endothelial cells was suggested to evaluate for NPs when used as therapeutics and diagnostics.

Yipeng Ye - One of the best experts on this subject based on the ideXlab platform.

  • core shell structure carbon coated Ferric Oxide fe2o3 c nanoparticles for supercapacitors with superior electrochemical performance
    Journal of Alloys and Compounds, 2015
    Co-Authors: Yipeng Ye, Haiyan Zhang, Yiming Chen, Peng Deng, Zhikun Huang, Yannan Qian, Yunyong Li, Qingyu Li
    Abstract:

    Abstract Core–shell structure carbon coated Ferric Oxide nanoparticles (Fe 2 O 3 @C) were fabricated by the oxidation of carbon coated iron nanoparticles (Fe@C) prepared by a direct current carbon arc discharge method. Porous activated-Fe 2 O 3 @C was prepared by KOH activation of Fe 2 O 3 @C at the temperature of 750 °C. X-ray diffraction analysis (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were employed to characterize the structure and morphology of the Fe 2 O 3 @C and activated-Fe 2 O 3 @C. The specific surface area and pore size distribution of the samples were also tested. The activated-Fe 2 O 3 @C electrodes exhibited good electrochemical performance with a maximum specific capacitance of 612 F g −1 at the charge/discharge current density of 0.5 A g −1 with 5 M NaOH electrolyte. After 10,000 cycling DC tests at the charge/discharge current density of 4 A g −1 , a high level specific capacitance of 518 F g −1 was obtained (90.6% retention of the initial capacity), suggesting excellent long-term cycling stability.

  • Core–shell structure carbon coated Ferric Oxide (fe2o3@C) nanoparticles for supercapacitors with superior electrochemical performance
    Journal of Alloys and Compounds, 2015
    Co-Authors: Yipeng Ye, Haiyan Zhang, Yiming Chen, Peng Deng, Zhikun Huang, Yannan Qian, Yunyong Li, Qingyu Li
    Abstract:

    Abstract Core–shell structure carbon coated Ferric Oxide nanoparticles (Fe 2 O 3 @C) were fabricated by the oxidation of carbon coated iron nanoparticles (Fe@C) prepared by a direct current carbon arc discharge method. Porous activated-Fe 2 O 3 @C was prepared by KOH activation of Fe 2 O 3 @C at the temperature of 750 °C. X-ray diffraction analysis (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were employed to characterize the structure and morphology of the Fe 2 O 3 @C and activated-Fe 2 O 3 @C. The specific surface area and pore size distribution of the samples were also tested. The activated-Fe 2 O 3 @C electrodes exhibited good electrochemical performance with a maximum specific capacitance of 612 F g −1 at the charge/discharge current density of 0.5 A g −1 with 5 M NaOH electrolyte. After 10,000 cycling DC tests at the charge/discharge current density of 4 A g −1 , a high level specific capacitance of 518 F g −1 was obtained (90.6% retention of the initial capacity), suggesting excellent long-term cycling stability.

Lingyu Piao - One of the best experts on this subject based on the ideXlab platform.

  • Comparative study of in vitro effects of different nanoparticles at non-cytotoxic concentration on the adherens junction of human vascular endothelial cells
    International Journal of Nanomedicine, 2019
    Co-Authors: Aiyun Yang, Lifan Du, Lingyu Piao, Jie Meng, Haiyan Xu
    Abstract:

    Effects of different nanoparticles (NPs) exposure at acutely non-cytotoxic concentrations are particularly worthy to figure out, compare, and elucidate. To investigate and compare the effect of a small library of NPs at non-cytotoxic concentration on the adherens junction of human umbilical vein endothelial cells (HUVECs), obtaining new insights of NPs safety evaluation. The HUVECs layer was exposed to NPs including gold (Au), platinum (Pt), silica (SiO2), titanium diOxide (TiO2), Ferric Oxide (fe2o3), oxidized multi-walled carbon nanotubes, with different surface chemistry and size distribution. Cellular uptake of NPs was observed by transmission electron microscopy. and the cytotoxicity was determined by Cell Counting Kit-8 assay. The NP-induced variation of intracellular reactive oxygen species (ROS) and catalase (CAT) activity was measured using the probe of 2'7'-dichlorodihydr fluorescein diacetate and a CAT analysis kit, respectively. The level of VE-cadherin of HUVECs was analyzed by Western blot, and the loss of adherens junction was observed with laser confocal microscopy. The acutely non-cytotoxic concentrations of different NPs were determined and applied to HUVECs. The NPs increased the level of intracellular ROS and the activity of CAT to different degrees, depending on the characteristics. At the same time, the HUVECs lost their adherens junction protein VE-cadherin and gaps were formed between the cells. The NP-induced oxidative stress and gap formation could be rescued by the supplementary N-acetylcysteine in the incubation. The increase of intracellular ROS and CAT activity was one common effect of NPs, even at the non-cytotoxic concentration, and the degree was dependent on the composition, surface chemistry, and size distribution of the NP. The effect led to the gap formation between the cells, while could be rescued by the antioxidant. Therefore, the variation of adherens junction between endothelial cells was suggested to evaluate for NPs when used as therapeutics and diagnostics.

  • comparative study of in vitro effects of different nanoparticles at non cytotoxic concentration on the adherens junction of human vascular endothelial cells
    International Journal of Nanomedicine, 2019
    Co-Authors: Aiyun Yang, Lifan Du, Lingyu Piao, Jie Meng, Haiyan Xu
    Abstract:

    Background: Effects of different nanoparticles (NPs) exposure at acutely non-cytotoxic concentrations are particularly worthy to figure out, compare, and elucidate. Objective: To investigate and compare the effect of a small library of NPs at non-cytotoxic concentration on the adherens junction of human umbilical vein endothelial cells (HUVECs), obtaining new insights of NPs safety evaluation. Materials and methods: The HUVECs layer was exposed to NPs including gold (Au), platinum (Pt), silica (SiO2), titanium diOxide (TiO2), Ferric Oxide (fe2o3), oxidized multi-walled carbon nanotubes, with different surface chemistry and size distribution. Cellular uptake of NPs was observed by transmission electron microscopy. and the cytotoxicity was determined by Cell Counting Kit-8 assay. The NP-induced variation of intracellular reactive oxygen species (ROS) and catalase (CAT) activity was measured using the probe of 2'7'-dichlorodihydr fluorescein diacetate and a CAT analysis kit, respectively. The level of VE-cadherin of HUVECs was analyzed by Western blot, and the loss of adherens junction was observed with laser confocal microscopy. Results: The acutely non-cytotoxic concentrations of different NPs were determined and applied to HUVECs. The NPs increased the level of intracellular ROS and the activity of CAT to different degrees, depending on the characteristics. At the same time, the HUVECs lost their adherens junction protein VE-cadherin and gaps were formed between the cells. The NP-induced oxidative stress and gap formation could be rescued by the supplementary N-acetylcysteine in the incubation. Conclusion: The increase of intracellular ROS and CAT activity was one common effect of NPs, even at the non-cytotoxic concentration, and the degree was dependent on the composition, surface chemistry, and size distribution of the NP. The effect led to the gap formation between the cells, while could be rescued by the antioxidant. Therefore, the variation of adherens junction between endothelial cells was suggested to evaluate for NPs when used as therapeutics and diagnostics.