The Experts below are selected from a list of 21 Experts worldwide ranked by ideXlab platform
Ryuzo Yanagimachi - One of the best experts on this subject based on the ideXlab platform.
-
Germ cell research: a personal perspective.
Biology of reproduction, 2008Co-Authors: Ryuzo YanagimachiAbstract:My interest in germ cells began when I first witnessed sea urchin Fertilization and embryo development during a laboratory class at Hokkaido University, Sapporo, Japan, almost 60 yr ago. Weismann's concept of germ cells that I learned during my undergraduate years became the driving force of my entire research career. During the early years, my associates and I used mainly the golden hamster and the guinea pig as model animals because their spermatozoa had large acrosomes and we could readily follow changes in the acrosomes without killing or staining spermatozoa. We later used the mouse as our model organism because we wanted to produce live offspring with known genetic backgrounds. A summary of the findings we made during those years includes the following: (1) first in vitro sperm capacitation; (2) discovery of sperm hyperactivation; (3) demonstration of the importance of Ca(2+) in sperm acrosome reaction, hyperactivation, sperm-egg fusion, and egg activation; (4) development of the sperm's fusion competence during the acrosome reaction; (5) characterization of sperm-oviduct relationships before and during Fertilization; (6) use of zona-free hamster eggs to examine fertilizing ability and chromosomes of human spermatozoa; (7) development and use of intracytoplasmic sperm injection; (8) use of prespermatozoal cells for the production of offspring; (9) sperm preservation by freeze-drying and freezing whole-animal bodies; and (10) mouse cloning by somatic cell nuclear transfer. My current interests and my visions for the future include the following: (1) mass production of mature eggs and spermatozoa in vitro, (2) permanent sperm preservation at ambient temperature, (3) development of safer and more efficient assisted Fertilization (Reproduction) technologies, (4) development of safe and efficient methods of cloning, (5) production of artificial organs such as an artificial uterus, (6) development of safe and effective male contraceptives, and (7) prevention of cancer through germ cell research.
Sandra Kraljević Pavelić - One of the best experts on this subject based on the ideXlab platform.
-
Mass spectrometry-based glycomic profiling of the total IgG and total proteome N-glycomes isolated from follicular fluid.
Biomolecular concepts, 2020Co-Authors: Marko Klobučar, Sanja Dević Pavlić, Iris Car, Neda Smiljan Severinski, Tamara Tramišak Milaković, Anđelka Radojčić Badovinac, Sandra Kraljević PavelićAbstract:Couples with infertility issues have been assisted by in vitro Fertilization Reproduction technologies with high success rates of 50-80%. However, complications associated with ovarian stimulation remain, such as ovarian hyperstimulation. Oocyte quality is a significant factor impacting the outcome of in vitro Fertilization procedures, but other processes are also critical for Fertilization success. Increasing evidence points to aberrant inflammation as one of these critical processes reflected in molecular changes, including glycosylation of proteins. Here we report results from a MALDI-TOF-MS-based glycomic profiling of the total IgG and total proteome N-glycomes isolated from the follicular fluid obtained from patients undergoing Fertilization through either (1) assisted Reproduction by modified natural cycle or (2) controlled ovarian stimulation (GnRH antagonist, GnRH Ant) protocols. Significant inflammatory-related differences between analyzed N-glycomes were observed from samples and correlated with the ovarian stimulation protocol used in patients.
Marko Klobučar - One of the best experts on this subject based on the ideXlab platform.
-
Mass spectrometry-based glycomic profiling of the total IgG and total proteome N-glycomes isolated from follicular fluid.
Biomolecular concepts, 2020Co-Authors: Marko Klobučar, Sanja Dević Pavlić, Iris Car, Neda Smiljan Severinski, Tamara Tramišak Milaković, Anđelka Radojčić Badovinac, Sandra Kraljević PavelićAbstract:Couples with infertility issues have been assisted by in vitro Fertilization Reproduction technologies with high success rates of 50-80%. However, complications associated with ovarian stimulation remain, such as ovarian hyperstimulation. Oocyte quality is a significant factor impacting the outcome of in vitro Fertilization procedures, but other processes are also critical for Fertilization success. Increasing evidence points to aberrant inflammation as one of these critical processes reflected in molecular changes, including glycosylation of proteins. Here we report results from a MALDI-TOF-MS-based glycomic profiling of the total IgG and total proteome N-glycomes isolated from the follicular fluid obtained from patients undergoing Fertilization through either (1) assisted Reproduction by modified natural cycle or (2) controlled ovarian stimulation (GnRH antagonist, GnRH Ant) protocols. Significant inflammatory-related differences between analyzed N-glycomes were observed from samples and correlated with the ovarian stimulation protocol used in patients.
Jan Zukal - One of the best experts on this subject based on the ideXlab platform.
-
Reproduction of Rescued Vespertilionid Bats (Nyctalus noctula) in Captivity: Veterinary and Physiologic Aspects
The veterinary clinics of North America. Exotic animal practice, 2017Co-Authors: Jiri Pikula, Hana Bandouchova, Veronika Kovacova, Petr Linhart, Vladimir Piacek, Jan ZukalAbstract:Long-term conservation and educational activities of numerous nongovernmental organizations have greatly increased public awareness about bats and their lifestyle. As a result, there is growing public concern about threats to bat populations. Many species of bats declined over recent decades and there is great demand for medical services to help injured or diseased bats. Veterinary clinicians dealing with such cases have to consider many issues, including ethical issues associated with the delayed Fertilization Reproduction strategy of temperate insectivorous bats. An outline of veterinary and physiologic requirements for treatment of and keeping vespertilionid bats in captivity is highlighted.
Iris Car - One of the best experts on this subject based on the ideXlab platform.
-
Mass spectrometry-based glycomic profiling of the total IgG and total proteome N-glycomes isolated from follicular fluid.
Biomolecular concepts, 2020Co-Authors: Marko Klobučar, Sanja Dević Pavlić, Iris Car, Neda Smiljan Severinski, Tamara Tramišak Milaković, Anđelka Radojčić Badovinac, Sandra Kraljević PavelićAbstract:Couples with infertility issues have been assisted by in vitro Fertilization Reproduction technologies with high success rates of 50-80%. However, complications associated with ovarian stimulation remain, such as ovarian hyperstimulation. Oocyte quality is a significant factor impacting the outcome of in vitro Fertilization procedures, but other processes are also critical for Fertilization success. Increasing evidence points to aberrant inflammation as one of these critical processes reflected in molecular changes, including glycosylation of proteins. Here we report results from a MALDI-TOF-MS-based glycomic profiling of the total IgG and total proteome N-glycomes isolated from the follicular fluid obtained from patients undergoing Fertilization through either (1) assisted Reproduction by modified natural cycle or (2) controlled ovarian stimulation (GnRH antagonist, GnRH Ant) protocols. Significant inflammatory-related differences between analyzed N-glycomes were observed from samples and correlated with the ovarian stimulation protocol used in patients.