The Experts below are selected from a list of 120 Experts worldwide ranked by ideXlab platform

Ellen M. Rasch - One of the best experts on this subject based on the ideXlab platform.

  • the dna content of trophozoites and cysts of giardia lamblia by microdensitometric quantitation of Feulgen Staining and examination by laser scanning confocal microscopy
    Journal of Histochemistry and Cytochemistry, 1994
    Co-Authors: Stanley L Erlandsen, Ellen M. Rasch
    Abstract:

    We investigated direct measurement of the DNA content of the parasitic intestinal flagellate Giardia lamblia through quantitation by Feulgen microspectrophotometry and also by visualization of Feulgen-stained DNA chromosomes within dividing cells by laser scanning confocal microscopy. Individual trophozoites of Giardia (binucleate) contained 0.144 +/- 0.018 pg of DNA/cell or 0.072 pg DNA/nucleus. Giardia lamblia cysts (quadranucleate) contained 0.313 +/- 0.003 pg DNA or 0.078 pg DNA/nucleus. The genome size (C) value per nucleus ranged between 6.5-7.1 x 10(7) BP for trophozoites and cysts, respectively. Confocal microscopic examination of Giardia trophozoites undergoing binary fission revealed five chromosome-like bodies within each nucleus. Further information about genome size and DNA content within different Giardia species may help to clarify the pivotal role of these primitive eukaryotic cells in evolutionary development.

Stanley L Erlandsen - One of the best experts on this subject based on the ideXlab platform.

  • the dna content of trophozoites and cysts of giardia lamblia by microdensitometric quantitation of Feulgen Staining and examination by laser scanning confocal microscopy
    Journal of Histochemistry and Cytochemistry, 1994
    Co-Authors: Stanley L Erlandsen, Ellen M. Rasch
    Abstract:

    We investigated direct measurement of the DNA content of the parasitic intestinal flagellate Giardia lamblia through quantitation by Feulgen microspectrophotometry and also by visualization of Feulgen-stained DNA chromosomes within dividing cells by laser scanning confocal microscopy. Individual trophozoites of Giardia (binucleate) contained 0.144 +/- 0.018 pg of DNA/cell or 0.072 pg DNA/nucleus. Giardia lamblia cysts (quadranucleate) contained 0.313 +/- 0.003 pg DNA or 0.078 pg DNA/nucleus. The genome size (C) value per nucleus ranged between 6.5-7.1 x 10(7) BP for trophozoites and cysts, respectively. Confocal microscopic examination of Giardia trophozoites undergoing binary fission revealed five chromosome-like bodies within each nucleus. Further information about genome size and DNA content within different Giardia species may help to clarify the pivotal role of these primitive eukaryotic cells in evolutionary development.

Martin Schramm - One of the best experts on this subject based on the ideXlab platform.

  • Feulgen Staining Remains the Gold Standard for Precise DNA Image Cytometry
    Anticancer Research, 2020
    Co-Authors: Stefan Biesterfeld, Sascha Beckers, Maria Del Carmen Villa Cadenas, Martin Schramm
    Abstract:

    Aim: The suitability of Papanicolaou Staining and of hematoxylin Staining for DNA single-cell cytometry was investigated in comparison to Feulgen Staining. Materials and Methods: Ten normal cervical smears and ten cervical smears containing cells of a squamous cell carcinoma in situ were analyzed. The integrated optical density (IOD) of 200 epithelial cells, chosen per random, was determined using a CM-1 TV-image analysis system (Hund, Wetzlar, Germany). Various DNA cytometric variables, accepted by the European Society for Analytical and Cellular Pathology (ESACP), and the mean nuclear area were calculated. Two measurements were performed after Papanicolaou Staining (wavelengths: 530 nm and 590 nm), followed by measurements after hematoxylin reStaining (wavelength: 590 nm) and after Feulgen reStaining (wavelength: 570 nm). Results: All histograms of Feulgen-stained normal squamous epithelia revealed a regular DNA distribution. The corresponding histograms after Papanicolaou Staining or hematoxylin Staining showed a wide scatter of values instead of a clear-cut diploid peak and an increased number of values >4c. Similar findings were observed in the carcinomatous smears. In particular, the mean values of the dispersion parameters (2cDI, entropy, ploidy imbalance and 2,5cEE) were significantly increased as compared to Feulgen Staining. Conclusion: Diagnostic or prognostic conclusions cannot be drawn from DNA measurements on Papanicolaou-stained or hematoxylin-stained specimens; Feulgen Staining remains the gold standard for such purposes.

  • Feulgen Staining remains the gold standard for precise dna image cytometry
    Anticancer Research, 2011
    Co-Authors: Stefan Biesterfeld, Sascha Beckers, Maria Del Carmen Villa Cadenas, Martin Schramm
    Abstract:

    AIM: The suitability of Papanicolaou Staining and of hematoxylin Staining for DNA single-cell cytometry was investigated in comparison to Feulgen Staining. MATERIALS AND METHODS: Ten normal cervical smears and ten cervical smears containing cells of a squamous cell carcinoma in situ were analyzed. The integrated optical density (IOD) of 200 epithelial cells, chosen per random, was determined using a CM-1 TV-image analysis system (Hund, Wetzlar, Germany). Various DNA cytometric variables, accepted by the European Society for Analytical and Cellular Pathology (ESACP), and the mean nuclear area were calculated. Two measurements were performed after Papanicolaou Staining (wavelengths: 530 nm and 590 nm), followed by measurements after hematoxylin re-Staining (wavelength: 590 nm) and after Feulgen reStaining (wavelength: 570 nm). RESULTS: All histograms of Feulgen-stained normal squamous epithelia revealed a regular DNA distribution. The corresponding histograms after Papanicolaou Staining or hematoxylin Staining showed a wide scatter of values instead of a clear-cut diploid peak and an increased number of values >4c. Similar findings were observed in the carcinomatous smears. In particular, the mean values of the dispersion parameters (2cDI, entropy, ploidy imbalance and 2,5cEE) were significantly increased as compared to Feulgen Staining. CONCLUSION: Diagnostic or prognostic conclusions cannot be drawn from DNA measurements on Papanicolaou-stained or hematoxylin-stained specimens; Feulgen Staining remains the gold standard for such purposes.

Elena V. Gostjeva - One of the best experts on this subject based on the ideXlab platform.

  • The study of calcified atherosclerotic arteries: an alternative to evaluate the composition of a problematic tissue reveals new insight including metakaryotic cells
    BMC Clinical Pathology, 2016
    Co-Authors: Silvia Fittipaldi, Francesco Vasuri, Alessio Degiovanni, Rodolfo Pini, Mauro Gargiulo, Andrea Stella, Gianandrea Pasquinelli, William G. Thilly, Elena V. Gostjeva
    Abstract:

    Background Calcifications of atherosclerotic plaques represent a controversial issue as they either lead to the stabilization or rupture of the lesion. However, the cellular key players involved in the progression of the calcified plaques have not yet been described. The primary reason for this lacuna is that decalcification procedures impair protein and nucleic acids contained in the calcified tissue. The aim of our study was to preserve the cellular content of heavily calcified plaques with a new rapid fixation in order to simplify the study of calcifications. Methods Here we applied a fixation method for fresh calcified tissue using the Carnoy’s solution followed by an enzymatic tissue digestion with type II collagenase. Immunohistochemistry was performed to verify the preservation of nuclear and cytoplasmic antigens. DNA content and RNA preservation was evaluated respectively with Feulgen Staining and RT-PCR. A checklist of steps for successful image analysis was provided. To present the basic features of the F-DNA analysis we used descriptive statistics, skewness and kurtosis. Differences in DNA content were analysed with Kruskal-Wallis and Dunn’s post tests. The value of P  

Danielle Pechinot - One of the best experts on this subject based on the ideXlab platform.

  • a combined agnor Feulgen Staining technique
    Journal of Histochemistry and Cytochemistry, 1990
    Co-Authors: Jean Foucrier, Jean Paul Rigaut, Danielle Pechinot
    Abstract:

    : We describe a new Staining technique (H-Ag-S) which allows observation and counting of active nucleolus organizer regions (NORs) and evaluation of the amount of DNA in the same cell nucleus. The procedure consists of combining a modified AgNOR Staining method with the Feulgen reaction. A sequential procedure is proposed, based on the determination of optimal Staining conditions. The technique, which was designed to allow studies of correlations between the transcriptional activity of rDNA genes and the cell ploidy, was primarily developed for rat liver smears. It should be applicable to most biological preparations, but the optimal conditions might be variable.