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Pietro Castellino - One of the best experts on this subject based on the ideXlab platform.

  • effects of dietary protein restriction on albumin and Fibrinogen Synthesis in macroalbuminuric type 2 diabetic patients
    Diabetologia, 2008
    Co-Authors: Mauro Giordano, Paola Lucidi, Pietro Castellino, Tiziana Ciarambino, L Gesue, M Cioffi, P Gresele, Giuseppe Paolisso, Pierpaolo De Feo
    Abstract:

    Aims/hypothesis Diabetic nephropathy is associated with hypoalbuminaemia and hyperFibrinogenaemia. A low-protein diet has been recommended in patients with diabetic nephropathy, but its effects on albumin and Fibrinogen Synthesis are unknown.

  • increased albumin and Fibrinogen Synthesis in hemodialysis patients with normal nutritional status
    Journal of The American Society of Nephrology, 2001
    Co-Authors: Mauro Giordano, Pierpaolo De Feo, Paola Lucidi, Emanuela Depascale, Gelsomina Giordano, Lorena Infantone, Anna Maria Zoccolo, Pietro Castellino
    Abstract:

    Abstract . This study compared the rates of whole-body proteolysis and of albumin and Fibrinogen Synthesis of seven hemodialysis patients (HD) with those of seven normal matched control subjects (C). HD patients had a normal nutritional and inflammatory status and serum albumin levels >3.5 g/dl. Endogenous leucine flux, albumin and Fibrinogen fractional Synthesis rate (FSR), and absolute intravascular Synthesis rate (ASR) of albumin and Fibrinogen all were evaluated by a primed/continuous infusion of 5,5,5-D3-L-leucine. Plasma volume was determined by the Evans blue dye dilution method. Endogenous leucine flux was significantly increased in HD (2.64 ± 0.08 μmol/kg per min) compared with C (2.17 ± 0.07 μmol/kg per min, P P P versus 114 ± 3 g/1.73 m 2 , P versus 6.7 ± 0.5 g/1.73 m 2 , P versus 10.3 ± 1 g/1.73 m 2 per d, P versus 1.94 ± 0.3 g/1.73 m 2 per d, P

  • Effects of dietary protein restriction on Fibrinogen and albumin metabolism in nephrotic patients
    Kidney international, 2001
    Co-Authors: Mauro Giordano, Pierpaolo De Feo, Paola Lucidi, Emanuela Depascale, Gelsomina Giordano, Domenico Cirillo, Giuseppe Dardo, Salvatore Santo Signorelli, Pietro Castellino
    Abstract:

    Effects of dietary protein restriction on Fibrinogen and albumin metabolism in nephrotic patients. Background Nephrotic syndrome (NS) is characterized by profound changes in albumin and Fibrinogen levels. Dietary protein restriction has been advocated in the treatment of patients with NS, but its effects on albumin and Fibrinogen metabolism have not been fully elucidated. Methods We evaluated the effects of dietary protein restriction on endogenous leucine flux (ELF), Fibrinogen and albumin metabolism in seven patients with NS who consumed either a normal protein diet (NPD; 1.20 ± 0.06 g/kg/day), or a low protein diet (LPS; 0.66 ± 0.04 g/kg/day) for four weeks. Seven normal subjects served as controls. The postabsorptive ELF value, fractional Synthesis rate (FSR) and absolute Synthesis rate (ASR) of both albumin and Fibrinogen were evaluated during the last 120 minutes of a five-hour 5,5,5-D3- L -leucine infusion. Results During the NPD regimen. ELF was increased, serum albumin was reduced, plasma Fibrinogen was increased, albumin FSR and ASR were both increased, Fibrinogen FSR was normal, and Fibrinogen ASR was greater in patients with NS compared to controls. In patients with NS the LPD regimen reduced proteinuria, ELF, albumin FSR and ASR, plasma Fibrinogen levels, Fibrinogen ASR, and increased serum ulbumin levels. Dietary-induced changes in albumin and Fibrinogen Synthesis were significantly correlated ( r = 0.719, P Conclusions Patients with NS treated with LPD show: ( 1 ) a reduction of proteinuria, albumin ASR and FSR, with an increase in serum albumin levels and its intravascular pool; ( 2 ) a decrease of Fibrinogen ASR, with a reduction in both plasma Fibrinogen levels and intravascular pool; and ( 3 ) a reduced rate of whole body proteolysis.

Pierpaolo De Feo - One of the best experts on this subject based on the ideXlab platform.

  • effects of dietary protein restriction on albumin and Fibrinogen Synthesis in macroalbuminuric type 2 diabetic patients
    Diabetologia, 2008
    Co-Authors: Mauro Giordano, Paola Lucidi, Pietro Castellino, Tiziana Ciarambino, L Gesue, M Cioffi, P Gresele, Giuseppe Paolisso, Pierpaolo De Feo
    Abstract:

    Aims/hypothesis Diabetic nephropathy is associated with hypoalbuminaemia and hyperFibrinogenaemia. A low-protein diet has been recommended in patients with diabetic nephropathy, but its effects on albumin and Fibrinogen Synthesis are unknown.

  • increased albumin and Fibrinogen Synthesis in hemodialysis patients with normal nutritional status
    Journal of The American Society of Nephrology, 2001
    Co-Authors: Mauro Giordano, Pierpaolo De Feo, Paola Lucidi, Emanuela Depascale, Gelsomina Giordano, Lorena Infantone, Anna Maria Zoccolo, Pietro Castellino
    Abstract:

    Abstract . This study compared the rates of whole-body proteolysis and of albumin and Fibrinogen Synthesis of seven hemodialysis patients (HD) with those of seven normal matched control subjects (C). HD patients had a normal nutritional and inflammatory status and serum albumin levels >3.5 g/dl. Endogenous leucine flux, albumin and Fibrinogen fractional Synthesis rate (FSR), and absolute intravascular Synthesis rate (ASR) of albumin and Fibrinogen all were evaluated by a primed/continuous infusion of 5,5,5-D3-L-leucine. Plasma volume was determined by the Evans blue dye dilution method. Endogenous leucine flux was significantly increased in HD (2.64 ± 0.08 μmol/kg per min) compared with C (2.17 ± 0.07 μmol/kg per min, P P P versus 114 ± 3 g/1.73 m 2 , P versus 6.7 ± 0.5 g/1.73 m 2 , P versus 10.3 ± 1 g/1.73 m 2 per d, P versus 1.94 ± 0.3 g/1.73 m 2 per d, P

  • Effects of dietary protein restriction on Fibrinogen and albumin metabolism in nephrotic patients
    Kidney international, 2001
    Co-Authors: Mauro Giordano, Pierpaolo De Feo, Paola Lucidi, Emanuela Depascale, Gelsomina Giordano, Domenico Cirillo, Giuseppe Dardo, Salvatore Santo Signorelli, Pietro Castellino
    Abstract:

    Effects of dietary protein restriction on Fibrinogen and albumin metabolism in nephrotic patients. Background Nephrotic syndrome (NS) is characterized by profound changes in albumin and Fibrinogen levels. Dietary protein restriction has been advocated in the treatment of patients with NS, but its effects on albumin and Fibrinogen metabolism have not been fully elucidated. Methods We evaluated the effects of dietary protein restriction on endogenous leucine flux (ELF), Fibrinogen and albumin metabolism in seven patients with NS who consumed either a normal protein diet (NPD; 1.20 ± 0.06 g/kg/day), or a low protein diet (LPS; 0.66 ± 0.04 g/kg/day) for four weeks. Seven normal subjects served as controls. The postabsorptive ELF value, fractional Synthesis rate (FSR) and absolute Synthesis rate (ASR) of both albumin and Fibrinogen were evaluated during the last 120 minutes of a five-hour 5,5,5-D3- L -leucine infusion. Results During the NPD regimen. ELF was increased, serum albumin was reduced, plasma Fibrinogen was increased, albumin FSR and ASR were both increased, Fibrinogen FSR was normal, and Fibrinogen ASR was greater in patients with NS compared to controls. In patients with NS the LPD regimen reduced proteinuria, ELF, albumin FSR and ASR, plasma Fibrinogen levels, Fibrinogen ASR, and increased serum ulbumin levels. Dietary-induced changes in albumin and Fibrinogen Synthesis were significantly correlated ( r = 0.719, P Conclusions Patients with NS treated with LPD show: ( 1 ) a reduction of proteinuria, albumin ASR and FSR, with an increase in serum albumin levels and its intravascular pool; ( 2 ) a decrease of Fibrinogen ASR, with a reduction in both plasma Fibrinogen levels and intravascular pool; and ( 3 ) a reduced rate of whole body proteolysis.

Mauro Giordano - One of the best experts on this subject based on the ideXlab platform.

  • effects of dietary protein restriction on albumin and Fibrinogen Synthesis in macroalbuminuric type 2 diabetic patients
    Diabetologia, 2008
    Co-Authors: Mauro Giordano, Paola Lucidi, Pietro Castellino, Tiziana Ciarambino, L Gesue, M Cioffi, P Gresele, Giuseppe Paolisso, Pierpaolo De Feo
    Abstract:

    Aims/hypothesis Diabetic nephropathy is associated with hypoalbuminaemia and hyperFibrinogenaemia. A low-protein diet has been recommended in patients with diabetic nephropathy, but its effects on albumin and Fibrinogen Synthesis are unknown.

  • increased albumin and Fibrinogen Synthesis in hemodialysis patients with normal nutritional status
    Journal of The American Society of Nephrology, 2001
    Co-Authors: Mauro Giordano, Pierpaolo De Feo, Paola Lucidi, Emanuela Depascale, Gelsomina Giordano, Lorena Infantone, Anna Maria Zoccolo, Pietro Castellino
    Abstract:

    Abstract . This study compared the rates of whole-body proteolysis and of albumin and Fibrinogen Synthesis of seven hemodialysis patients (HD) with those of seven normal matched control subjects (C). HD patients had a normal nutritional and inflammatory status and serum albumin levels >3.5 g/dl. Endogenous leucine flux, albumin and Fibrinogen fractional Synthesis rate (FSR), and absolute intravascular Synthesis rate (ASR) of albumin and Fibrinogen all were evaluated by a primed/continuous infusion of 5,5,5-D3-L-leucine. Plasma volume was determined by the Evans blue dye dilution method. Endogenous leucine flux was significantly increased in HD (2.64 ± 0.08 μmol/kg per min) compared with C (2.17 ± 0.07 μmol/kg per min, P P P versus 114 ± 3 g/1.73 m 2 , P versus 6.7 ± 0.5 g/1.73 m 2 , P versus 10.3 ± 1 g/1.73 m 2 per d, P versus 1.94 ± 0.3 g/1.73 m 2 per d, P

  • Effects of dietary protein restriction on Fibrinogen and albumin metabolism in nephrotic patients
    Kidney international, 2001
    Co-Authors: Mauro Giordano, Pierpaolo De Feo, Paola Lucidi, Emanuela Depascale, Gelsomina Giordano, Domenico Cirillo, Giuseppe Dardo, Salvatore Santo Signorelli, Pietro Castellino
    Abstract:

    Effects of dietary protein restriction on Fibrinogen and albumin metabolism in nephrotic patients. Background Nephrotic syndrome (NS) is characterized by profound changes in albumin and Fibrinogen levels. Dietary protein restriction has been advocated in the treatment of patients with NS, but its effects on albumin and Fibrinogen metabolism have not been fully elucidated. Methods We evaluated the effects of dietary protein restriction on endogenous leucine flux (ELF), Fibrinogen and albumin metabolism in seven patients with NS who consumed either a normal protein diet (NPD; 1.20 ± 0.06 g/kg/day), or a low protein diet (LPS; 0.66 ± 0.04 g/kg/day) for four weeks. Seven normal subjects served as controls. The postabsorptive ELF value, fractional Synthesis rate (FSR) and absolute Synthesis rate (ASR) of both albumin and Fibrinogen were evaluated during the last 120 minutes of a five-hour 5,5,5-D3- L -leucine infusion. Results During the NPD regimen. ELF was increased, serum albumin was reduced, plasma Fibrinogen was increased, albumin FSR and ASR were both increased, Fibrinogen FSR was normal, and Fibrinogen ASR was greater in patients with NS compared to controls. In patients with NS the LPD regimen reduced proteinuria, ELF, albumin FSR and ASR, plasma Fibrinogen levels, Fibrinogen ASR, and increased serum ulbumin levels. Dietary-induced changes in albumin and Fibrinogen Synthesis were significantly correlated ( r = 0.719, P Conclusions Patients with NS treated with LPD show: ( 1 ) a reduction of proteinuria, albumin ASR and FSR, with an increase in serum albumin levels and its intravascular pool; ( 2 ) a decrease of Fibrinogen ASR, with a reduction in both plasma Fibrinogen levels and intravascular pool; and ( 3 ) a reduced rate of whole body proteolysis.

Jean D Sipe - One of the best experts on this subject based on the ideXlab platform.

  • the down regulation of il 6 stimulated Fibrinogen steady state mrna and protein levels by human recombinant il 1 is not pge2 dependent effects of il 1 receptor antagonist il 1ra
    Molecular and Cellular Biochemistry, 1995
    Co-Authors: P Conti, Laura Bartle, Renato C Barbacane, Marcella Reale, Jean D Sipe
    Abstract:

    Infections, trauma and inflammatory processes induce a host response with increases in a large group of structurally and functionally diverse plasma proteins. Parental administration of foreign proteins also induce an increase in plasma Fibrinogen. Interleukin-6 (IL-6) is a monocyte-derived mediator and has regulatory effects on acute phase protein genes which result in the induction of Fibrinogen Synthesis in primary hepatocytes, while the addition of interleukin-1 (IL-1) exerts a negative modulating influence on the IL-6-stimulated Fibrinogen. In order to understand the mechanisms by which IL-1 inhibits IL-6-stimulated Fibrinogen transcription and translation, and since IL-1 is believed to act through PGE2 stimulation, we have studied the influence of PGE2 in IL-6 or IL-1, alone and in combination, on Fg mRNA expression (by Northern blot analysis) and the influence of PGE2, indomethacin, and arachidonic acid on Fg secretion. Moreover, since human recombinant interleukin-1 receptor antagonist (hrIL-1ra) is a strong inhibitor of IL-1 induced IL-1 transcription and translation and has an inhibitory effect on PGE2, we have studied the effects of IL-1ra on the down-regulation of IL-6 stimulated Fibrinogen by IL-1, using an Fg ELISA method.

  • increased Fibrinogen Synthesis in mice during the acute phase response co operative interaction of interleukin 1 interleukin 6 and interleukin 1 receptor antagonist
    Cytokine, 1993
    Co-Authors: Hanna Rokita, Ruta Neta, Jean D Sipe
    Abstract:

    Interleukin 6 (IL-6) stimulates Fibrinogen (Fg) gene expression both in vivo and in vitro; while interleukin 1 (IL-1) paradoxically stimulates in vivo, yet inhibits in vitro, Fg Synthesis. The naturally occurring interleukin 1 receptor antagonist (IL-1ra) and passive immunization with anti-IL-6 antiserum were used to study the in vivo mechanism of action of IL-1 on Fg gene expression. Changes in plasma Fg and hepatic Fg mRNA concentrations were measured following administration of exogenous IL-1ra together with IL-6 or IL-1 to CD2F1 mice. Our results suggest that in vivo, IL-1 per se inhibits Fg production since when IL-1ra was co-administered with IL-6, greater concentrations of Fg were observed than when IL-6 was administered alone. The data suggest that IL-1 stimulates Fg production through intermediate production of IL-6, since stimulation was abrogated when either IL-1ra or anti-IL-6 antiserum was co-administered with IL-1. An in vivo role for IL-1ra in the stimulation of Fg by IL-1 was supported by the observation that within 1 h of IL-1 administration to mice, IL-1ra mRNA was detectable in liver. It appears that IL-1, an early mediator of inflammation, inhibits constitutive expression of Fg genes and stimulates the IL-1ra and IL-6 genes. The inhibitory effect of IL-1 is reversed by endogenous IL-1ra and by the direct stimulation of Fg gene expression by IL-6.

Oliver Grottke - One of the best experts on this subject based on the ideXlab platform.

  • Testing in a 24-Hour Porcine Trauma Model
    2016
    Co-Authors: Christian Zentai, Jonas Schnabel, Rolf Rossaint, Cristina Solomon, Henri M. H. Spronk, Oliver Grottke
    Abstract:

    Introduction: In a 24-hour porcine model of liver injury, we showed that Fibrinogen supplementation does not downregulate endogenous Fibrinogen Synthesis. Herewe report data from the same study showing the impact of Fibrinogen on coagulation variables. Materials and Methods: Coagulopathy was induced in 20 German land race pigs by hemodilution and blunt liver injury. Animals randomly received Fibrinogen concentrate (100 mg/kg) or saline. Coagulation parameters were assessed and thromboelastometry (ROTEM) was performed. Results: Fibrinogen concentrate significantly reduced the prolongations of EXTEM clotting time, EXTE

  • Fibrinogen Concentrate Does Not Suppress Endogenous Fibrinogen Synthesis in a 24-hour Porcine Trauma Model
    Anesthesiology, 2014
    Co-Authors: Christian Zentai, Till Braunschweig, Jonas Schnabel, Michael Rose, Rolf Rossaint, Oliver Grottke
    Abstract:

    BACKGROUND Fibrinogen concentrate may reduce blood loss after trauma. However, its effect on endogenous Fibrinogen Synthesis is unknown. The authors investigated the effect of exogenous human Fibrinogen on endogenous Fibrinogen metabolism in a 24-h porcine trauma model. METHODS Coagulopathy was induced in 20 German Landrace pigs by hemodilution and blunt liver injury. Animals were randomized to receive Fibrinogen concentrate (100 mg/kg; infusion beginning 20 min postinjury and lasting approximately 10 min) or saline. Fibrinogen concentration, thromboelastometry, and quantitative reverse transcriptase polymerase chain reaction of Fibrinogen genes in liver tissue samples were recorded. Internal organs were examined histologically for emboli. RESULTS Coagulation parameters were impaired and plasma Fibrinogen concentrations were reduced before starting infusion of Fibrinogen concentrate/saline. Twenty minutes after starting infusion, exogenous Fibrinogen supplementation had increased plasma Fibrinogen concentration versus controls (171 ± 19 vs. 63 ± 10 mg/dl [mean ± SD for Multifibren U]; 185 ± 30 vs. 41 ± 4 mg/dl [Thrombin reagent]; P < 0.05 for both comparisons). The between-group difference in plasma Fibrinogen concentration diminished thereafter, with maximum concentrations in both groups observed at approximately 24 h, that is, during the acute-phase reaction after trauma. Fibrinogen supplementation did not down-regulate endogenous Fibrinogen Synthesis (no between-group differences in Fibrinogen messenger RNA). Total postinjury blood loss was significantly lower in the Fibrinogen group (1,062 ± 216 vs. 1,643 ± 244 ml; P < 0.001). No signs of thromboembolism were observed. CONCLUSIONS Administration of human Fibrinogen concentrate did not down-regulate endogenous porcine Fibrinogen Synthesis. The effect on plasma Fibrinogen concentration was most pronounced at 20 min but nonsignificant at approximately 24 h.