The Experts below are selected from a list of 17892 Experts worldwide ranked by ideXlab platform
I. R. Rajput - One of the best experts on this subject based on the ideXlab platform.
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establishment of pantropic spotted dolphin stenella attenuata Fibroblast Cell Line and potential influence of polybrominated diphenyl ethers pbdes on cytokines response
Aquatic Toxicology, 2018Co-Authors: I. R. Rajput, Edmond Sanganyado, Sun Yajing, Ziyang Xiao, Summra Yaqoob, Huang YingAbstract:Abstract The presence of polybrominated diphenyl ethers (PBDEs) in the aquatic environment is an issue of major concern which may be a cause of increasing prevalence and severity of diseases in marine mammals. Although, Cell culture model development and in vitro investigation approach is a prime need of time to progress immunotoxic research on aquatic mammals. In this study, we stablished Fibroblast Cell Line (pantropic spotted dolphin) to assess the potential effects of PBDEs on cytokines response. Cells were grown in 6 well Cell culture plate and complete media (DMEM and Ham's F12 nutrient mixture, fetal bovine serum, antibiotic and essential amino acids) was provided. The primary culture of (PSP-LWH) Cells identification was achieved by vimentin (gene and protein) expressions. Karyotyping revealed pantropic spotted dolphin chromosomes 20 pairs with XX. Transfection was achieved by SV40 LT antigen and transfected Cells were expended for passages. Stability of Cell Line was confirmed at various passages intervals using RT-PCR, western blotting and immunofluorescence methods. After confirmation, Cell Line was exposed to BDE-47 (250 ng/ml), BDE-100 (250 ng/ml) and BDE-209 (1000 ng/ml), with control group (PBS), positive control DMSO (0.1%) and negative control LPS (500 ng/ml) for 24 h. The ELISA results showed significant increase in IL-6 in BDE- 100 and BDE-209 while IL-1β and IL-8 were found higher in BDE-47 and BDE-100. TNFα and IL-10 secretion was noted higher in control and positive control groups. Altogether, these results emphasize importance of transfected (PSP-LWHT) Cell Line in aquatic research and potential effects of PBDEs on Fibroblast provides evident to understand immune modulating effects of PBDEs in marine mammals. The impact of PBDEs on dolphin’s Fibroblast Cells immune response and altered cytokine response have been presented for the first time.
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establishment and characterization of pygmy killer whale feresa attenuata dermal Fibroblast Cell Line
PLOS ONE, 2018Co-Authors: Sun Yajing, Wang Jingzhen, I. R. Rajput, Edmond Sanganyado, Huang Ying, Li Ping, Liu WenhuaAbstract:The pygmy killer whale (Feresa attenuata) (PKW) is a tropical and subtropical marine mammal commonly found in the Atlantic, Indian and Pacific oceans. Since the PKWs live in offshore protected territories, they are rarely seen onshore. Hence, PKW are one of the most poorly understood oceanic species of odontocetes. The dermal tissue comes primarily from stranding events that occur along the coast of the Shantou, Guangdong, China. The sampled tissues were immediately processed and attached on collagen-coated 6-well tissue culture plate. The complete medium (DMEM and Ham’s F12, fetal bovine serum, antibiotic and essential amino acids) was added to the culture plates. The primary culture (PKW-LWH) Cells were verified as Fibroblast by vimentin and karyotype analyses, which revealed 42 autosomes and two sex chromosomes X and Y. Following transfection of PKW-LWH Cells with a plasmid encoding, the SV40 large T-antigens and the transfected Cells were isolated and expanded. Using RT-PCR, western blot, immunofluorescence analysis and SV40 large T-antigen stability was confirmed. The Cell proliferation rate of the Fibroblast Cells, PKW-LWHT was faster than the primary Cells PKW-LWH with the doubling time 68.9h and 14.4h, respectively. In this study, we established PKW dermal Fibroblast Cell Line for the first time, providing a unique opportunity for in vitro studies on the effects of environmental pollutants and pathogens that could be determined in PKW and/or Cetaceans.
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establishment and characterization of pygmy killer whale feresa attenuata dermal Fibroblast Cell Line
PLOS ONE, 2018Co-Authors: Sun Yajing, Wang Jingzhen, I. R. Rajput, Edmond Sanganyado, Huang Ying, Li Ping, Liu WenhuaAbstract:The pygmy killer whale (Feresa attenuata) (PKW) is a tropical and subtropical marine mammal commonly found in the Atlantic, Indian and Pacific oceans. Since the PKWs live in offshore protected territories, they are rarely seen onshore. Hence, PKW are one of the most poorly understood oceanic species of odontocetes. The dermal tissue comes primarily from stranding events that occur along the coast of the Shantou, Guangdong, China. The sampled tissues were immediately processed and attached on collagen-coated 6-well tissue culture plate. The complete medium (DMEM and Ham’s F12, fetal bovine serum, antibiotic and essential amino acids) was added to the culture plates. The primary culture (PKW-LWH) Cells were verified as Fibroblast by vimentin and karyotype analyses, which revealed 42 autosomes and two sex chromosomes X and Y. Following transfection of PKW-LWH Cells with a plasmid encoding, the SV40 large T-antigens and the transfected Cells were isolated and expanded. Using RT-PCR, western blot, immunofluorescence analysis and SV40 large T-antigen stability was confirmed. The Cell proliferation rate of the Fibroblast Cells, PKW-LWHT was faster than the primary Cells PKW-LWH with the doubling time 68.9h and 14.4h, respectively. In this study, we established PKW dermal Fibroblast Cell Line for the first time, providing a unique opportunity for in vitro studies on the effects of environmental pollutants and pathogens that could be determined in PKW and/or Cetaceans.
Liu Wenhua - One of the best experts on this subject based on the ideXlab platform.
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establishment and characterization of pygmy killer whale feresa attenuata dermal Fibroblast Cell Line
PLOS ONE, 2018Co-Authors: Sun Yajing, Wang Jingzhen, I. R. Rajput, Edmond Sanganyado, Huang Ying, Li Ping, Liu WenhuaAbstract:The pygmy killer whale (Feresa attenuata) (PKW) is a tropical and subtropical marine mammal commonly found in the Atlantic, Indian and Pacific oceans. Since the PKWs live in offshore protected territories, they are rarely seen onshore. Hence, PKW are one of the most poorly understood oceanic species of odontocetes. The dermal tissue comes primarily from stranding events that occur along the coast of the Shantou, Guangdong, China. The sampled tissues were immediately processed and attached on collagen-coated 6-well tissue culture plate. The complete medium (DMEM and Ham’s F12, fetal bovine serum, antibiotic and essential amino acids) was added to the culture plates. The primary culture (PKW-LWH) Cells were verified as Fibroblast by vimentin and karyotype analyses, which revealed 42 autosomes and two sex chromosomes X and Y. Following transfection of PKW-LWH Cells with a plasmid encoding, the SV40 large T-antigens and the transfected Cells were isolated and expanded. Using RT-PCR, western blot, immunofluorescence analysis and SV40 large T-antigen stability was confirmed. The Cell proliferation rate of the Fibroblast Cells, PKW-LWHT was faster than the primary Cells PKW-LWH with the doubling time 68.9h and 14.4h, respectively. In this study, we established PKW dermal Fibroblast Cell Line for the first time, providing a unique opportunity for in vitro studies on the effects of environmental pollutants and pathogens that could be determined in PKW and/or Cetaceans.
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establishment and characterization of pygmy killer whale feresa attenuata dermal Fibroblast Cell Line
PLOS ONE, 2018Co-Authors: Sun Yajing, Wang Jingzhen, I. R. Rajput, Edmond Sanganyado, Huang Ying, Li Ping, Liu WenhuaAbstract:The pygmy killer whale (Feresa attenuata) (PKW) is a tropical and subtropical marine mammal commonly found in the Atlantic, Indian and Pacific oceans. Since the PKWs live in offshore protected territories, they are rarely seen onshore. Hence, PKW are one of the most poorly understood oceanic species of odontocetes. The dermal tissue comes primarily from stranding events that occur along the coast of the Shantou, Guangdong, China. The sampled tissues were immediately processed and attached on collagen-coated 6-well tissue culture plate. The complete medium (DMEM and Ham’s F12, fetal bovine serum, antibiotic and essential amino acids) was added to the culture plates. The primary culture (PKW-LWH) Cells were verified as Fibroblast by vimentin and karyotype analyses, which revealed 42 autosomes and two sex chromosomes X and Y. Following transfection of PKW-LWH Cells with a plasmid encoding, the SV40 large T-antigens and the transfected Cells were isolated and expanded. Using RT-PCR, western blot, immunofluorescence analysis and SV40 large T-antigen stability was confirmed. The Cell proliferation rate of the Fibroblast Cells, PKW-LWHT was faster than the primary Cells PKW-LWH with the doubling time 68.9h and 14.4h, respectively. In this study, we established PKW dermal Fibroblast Cell Line for the first time, providing a unique opportunity for in vitro studies on the effects of environmental pollutants and pathogens that could be determined in PKW and/or Cetaceans.
Sun Yajing - One of the best experts on this subject based on the ideXlab platform.
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establishment of pantropic spotted dolphin stenella attenuata Fibroblast Cell Line and potential influence of polybrominated diphenyl ethers pbdes on cytokines response
Aquatic Toxicology, 2018Co-Authors: I. R. Rajput, Edmond Sanganyado, Sun Yajing, Ziyang Xiao, Summra Yaqoob, Huang YingAbstract:Abstract The presence of polybrominated diphenyl ethers (PBDEs) in the aquatic environment is an issue of major concern which may be a cause of increasing prevalence and severity of diseases in marine mammals. Although, Cell culture model development and in vitro investigation approach is a prime need of time to progress immunotoxic research on aquatic mammals. In this study, we stablished Fibroblast Cell Line (pantropic spotted dolphin) to assess the potential effects of PBDEs on cytokines response. Cells were grown in 6 well Cell culture plate and complete media (DMEM and Ham's F12 nutrient mixture, fetal bovine serum, antibiotic and essential amino acids) was provided. The primary culture of (PSP-LWH) Cells identification was achieved by vimentin (gene and protein) expressions. Karyotyping revealed pantropic spotted dolphin chromosomes 20 pairs with XX. Transfection was achieved by SV40 LT antigen and transfected Cells were expended for passages. Stability of Cell Line was confirmed at various passages intervals using RT-PCR, western blotting and immunofluorescence methods. After confirmation, Cell Line was exposed to BDE-47 (250 ng/ml), BDE-100 (250 ng/ml) and BDE-209 (1000 ng/ml), with control group (PBS), positive control DMSO (0.1%) and negative control LPS (500 ng/ml) for 24 h. The ELISA results showed significant increase in IL-6 in BDE- 100 and BDE-209 while IL-1β and IL-8 were found higher in BDE-47 and BDE-100. TNFα and IL-10 secretion was noted higher in control and positive control groups. Altogether, these results emphasize importance of transfected (PSP-LWHT) Cell Line in aquatic research and potential effects of PBDEs on Fibroblast provides evident to understand immune modulating effects of PBDEs in marine mammals. The impact of PBDEs on dolphin’s Fibroblast Cells immune response and altered cytokine response have been presented for the first time.
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establishment and characterization of pygmy killer whale feresa attenuata dermal Fibroblast Cell Line
PLOS ONE, 2018Co-Authors: Sun Yajing, Wang Jingzhen, I. R. Rajput, Edmond Sanganyado, Huang Ying, Li Ping, Liu WenhuaAbstract:The pygmy killer whale (Feresa attenuata) (PKW) is a tropical and subtropical marine mammal commonly found in the Atlantic, Indian and Pacific oceans. Since the PKWs live in offshore protected territories, they are rarely seen onshore. Hence, PKW are one of the most poorly understood oceanic species of odontocetes. The dermal tissue comes primarily from stranding events that occur along the coast of the Shantou, Guangdong, China. The sampled tissues were immediately processed and attached on collagen-coated 6-well tissue culture plate. The complete medium (DMEM and Ham’s F12, fetal bovine serum, antibiotic and essential amino acids) was added to the culture plates. The primary culture (PKW-LWH) Cells were verified as Fibroblast by vimentin and karyotype analyses, which revealed 42 autosomes and two sex chromosomes X and Y. Following transfection of PKW-LWH Cells with a plasmid encoding, the SV40 large T-antigens and the transfected Cells were isolated and expanded. Using RT-PCR, western blot, immunofluorescence analysis and SV40 large T-antigen stability was confirmed. The Cell proliferation rate of the Fibroblast Cells, PKW-LWHT was faster than the primary Cells PKW-LWH with the doubling time 68.9h and 14.4h, respectively. In this study, we established PKW dermal Fibroblast Cell Line for the first time, providing a unique opportunity for in vitro studies on the effects of environmental pollutants and pathogens that could be determined in PKW and/or Cetaceans.
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establishment and characterization of pygmy killer whale feresa attenuata dermal Fibroblast Cell Line
PLOS ONE, 2018Co-Authors: Sun Yajing, Wang Jingzhen, I. R. Rajput, Edmond Sanganyado, Huang Ying, Li Ping, Liu WenhuaAbstract:The pygmy killer whale (Feresa attenuata) (PKW) is a tropical and subtropical marine mammal commonly found in the Atlantic, Indian and Pacific oceans. Since the PKWs live in offshore protected territories, they are rarely seen onshore. Hence, PKW are one of the most poorly understood oceanic species of odontocetes. The dermal tissue comes primarily from stranding events that occur along the coast of the Shantou, Guangdong, China. The sampled tissues were immediately processed and attached on collagen-coated 6-well tissue culture plate. The complete medium (DMEM and Ham’s F12, fetal bovine serum, antibiotic and essential amino acids) was added to the culture plates. The primary culture (PKW-LWH) Cells were verified as Fibroblast by vimentin and karyotype analyses, which revealed 42 autosomes and two sex chromosomes X and Y. Following transfection of PKW-LWH Cells with a plasmid encoding, the SV40 large T-antigens and the transfected Cells were isolated and expanded. Using RT-PCR, western blot, immunofluorescence analysis and SV40 large T-antigen stability was confirmed. The Cell proliferation rate of the Fibroblast Cells, PKW-LWHT was faster than the primary Cells PKW-LWH with the doubling time 68.9h and 14.4h, respectively. In this study, we established PKW dermal Fibroblast Cell Line for the first time, providing a unique opportunity for in vitro studies on the effects of environmental pollutants and pathogens that could be determined in PKW and/or Cetaceans.
Huang Ying - One of the best experts on this subject based on the ideXlab platform.
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establishment of pantropic spotted dolphin stenella attenuata Fibroblast Cell Line and potential influence of polybrominated diphenyl ethers pbdes on cytokines response
Aquatic Toxicology, 2018Co-Authors: I. R. Rajput, Edmond Sanganyado, Sun Yajing, Ziyang Xiao, Summra Yaqoob, Huang YingAbstract:Abstract The presence of polybrominated diphenyl ethers (PBDEs) in the aquatic environment is an issue of major concern which may be a cause of increasing prevalence and severity of diseases in marine mammals. Although, Cell culture model development and in vitro investigation approach is a prime need of time to progress immunotoxic research on aquatic mammals. In this study, we stablished Fibroblast Cell Line (pantropic spotted dolphin) to assess the potential effects of PBDEs on cytokines response. Cells were grown in 6 well Cell culture plate and complete media (DMEM and Ham's F12 nutrient mixture, fetal bovine serum, antibiotic and essential amino acids) was provided. The primary culture of (PSP-LWH) Cells identification was achieved by vimentin (gene and protein) expressions. Karyotyping revealed pantropic spotted dolphin chromosomes 20 pairs with XX. Transfection was achieved by SV40 LT antigen and transfected Cells were expended for passages. Stability of Cell Line was confirmed at various passages intervals using RT-PCR, western blotting and immunofluorescence methods. After confirmation, Cell Line was exposed to BDE-47 (250 ng/ml), BDE-100 (250 ng/ml) and BDE-209 (1000 ng/ml), with control group (PBS), positive control DMSO (0.1%) and negative control LPS (500 ng/ml) for 24 h. The ELISA results showed significant increase in IL-6 in BDE- 100 and BDE-209 while IL-1β and IL-8 were found higher in BDE-47 and BDE-100. TNFα and IL-10 secretion was noted higher in control and positive control groups. Altogether, these results emphasize importance of transfected (PSP-LWHT) Cell Line in aquatic research and potential effects of PBDEs on Fibroblast provides evident to understand immune modulating effects of PBDEs in marine mammals. The impact of PBDEs on dolphin’s Fibroblast Cells immune response and altered cytokine response have been presented for the first time.
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establishment and characterization of pygmy killer whale feresa attenuata dermal Fibroblast Cell Line
PLOS ONE, 2018Co-Authors: Sun Yajing, Wang Jingzhen, I. R. Rajput, Edmond Sanganyado, Huang Ying, Li Ping, Liu WenhuaAbstract:The pygmy killer whale (Feresa attenuata) (PKW) is a tropical and subtropical marine mammal commonly found in the Atlantic, Indian and Pacific oceans. Since the PKWs live in offshore protected territories, they are rarely seen onshore. Hence, PKW are one of the most poorly understood oceanic species of odontocetes. The dermal tissue comes primarily from stranding events that occur along the coast of the Shantou, Guangdong, China. The sampled tissues were immediately processed and attached on collagen-coated 6-well tissue culture plate. The complete medium (DMEM and Ham’s F12, fetal bovine serum, antibiotic and essential amino acids) was added to the culture plates. The primary culture (PKW-LWH) Cells were verified as Fibroblast by vimentin and karyotype analyses, which revealed 42 autosomes and two sex chromosomes X and Y. Following transfection of PKW-LWH Cells with a plasmid encoding, the SV40 large T-antigens and the transfected Cells were isolated and expanded. Using RT-PCR, western blot, immunofluorescence analysis and SV40 large T-antigen stability was confirmed. The Cell proliferation rate of the Fibroblast Cells, PKW-LWHT was faster than the primary Cells PKW-LWH with the doubling time 68.9h and 14.4h, respectively. In this study, we established PKW dermal Fibroblast Cell Line for the first time, providing a unique opportunity for in vitro studies on the effects of environmental pollutants and pathogens that could be determined in PKW and/or Cetaceans.
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establishment and characterization of pygmy killer whale feresa attenuata dermal Fibroblast Cell Line
PLOS ONE, 2018Co-Authors: Sun Yajing, Wang Jingzhen, I. R. Rajput, Edmond Sanganyado, Huang Ying, Li Ping, Liu WenhuaAbstract:The pygmy killer whale (Feresa attenuata) (PKW) is a tropical and subtropical marine mammal commonly found in the Atlantic, Indian and Pacific oceans. Since the PKWs live in offshore protected territories, they are rarely seen onshore. Hence, PKW are one of the most poorly understood oceanic species of odontocetes. The dermal tissue comes primarily from stranding events that occur along the coast of the Shantou, Guangdong, China. The sampled tissues were immediately processed and attached on collagen-coated 6-well tissue culture plate. The complete medium (DMEM and Ham’s F12, fetal bovine serum, antibiotic and essential amino acids) was added to the culture plates. The primary culture (PKW-LWH) Cells were verified as Fibroblast by vimentin and karyotype analyses, which revealed 42 autosomes and two sex chromosomes X and Y. Following transfection of PKW-LWH Cells with a plasmid encoding, the SV40 large T-antigens and the transfected Cells were isolated and expanded. Using RT-PCR, western blot, immunofluorescence analysis and SV40 large T-antigen stability was confirmed. The Cell proliferation rate of the Fibroblast Cells, PKW-LWHT was faster than the primary Cells PKW-LWH with the doubling time 68.9h and 14.4h, respectively. In this study, we established PKW dermal Fibroblast Cell Line for the first time, providing a unique opportunity for in vitro studies on the effects of environmental pollutants and pathogens that could be determined in PKW and/or Cetaceans.
Mengsu Yang - One of the best experts on this subject based on the ideXlab platform.
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herbal formula astragali radix and rehmanniae radix exerted wound healing effect on human skin Fibroblast Cell Line hs27 via the activation of transformation growth factor tgf β pathway and promoting extraCellular matrix ecm deposition
Phytomedicine, 2012Co-Authors: Qi Zhang, Chi Chun Fong, Yao Chen, Fan Wei, Chi Man Koon, Kit Man Lau, Pingchung Leung, Clara Biksan Lau, Kwokpui Fung, Mengsu YangAbstract:Astragali Radix (AR) and Rehmanniae Radix (RR) have long been used in traditional Chinese Medicine and as the principal herbs in treating diabetic foot ulcer. In this study, we investigated the effect of NF3, which comprises of AR and RR in the ratio of 2:1(w/w), on skin Fibroblast Cell migration and the activation of selected genes and proteins related to wound healing. Human skin Fibroblast Cell Line Hs27 was treated with NF3 at 4 mg/ml for 24h, and in vitro scratch wound healing and quantitative Cell migration assays were performed, respectively. The expression of transformation growth factor (TGF-β1) and bone morphogenetic protein 6 (BMP6) in Hs27 Cells with or without NF3 treatment was analyzed by western blot analysis. In addition, the expression of a panel of genes involved in human TGF-β signaling pathway was analyzed in Hs27 Cells upon NF3 treatment (4 mg/ml, 24 h) by quantitative real-time PCR (qRT-PCR). Furthermore, the expression of several genes and proteins associated with ECM synthesis was investigated by qRT-PCR analysis or/and ELISA techniques. The results suggested that NF3 promoted the migration of human skin Fibroblast Cells. Western blot analysis demonstrated that NF3 up-regulated TGF-β1 and BMP-6 synthesis. qRT-PCR analysis revealed that the expression of 26 genes in Hs27 Cells was changed upon NF3 induction, including TGF-β superfamily ligands and down stream effectors genes, and genes involved in TGF/Smad pathway, and Ras/MAPK (non-Smad) pathway. Among the extraCellular matrix (ECM)-related molecules, it was found that NF3 up-regulated the expression of type I and III collagens, fibronectin as well as TIMP-1, and down-regulated the MMP-9 expression in skin Fibroblast Cells. This study demonstrated that herb formula NF3 could enhance skin Fibroblast Cell migration and activated genes involved in TGF-β1 pathway. NF3 could regulate gene transcription for extraCellular matrix synthesis via the Smad pathway, and gene transcription for Cell motility via the Ras/MAPK (non-Smad) pathway.
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transcriptional profiling of human skin Fibroblast Cell Line hs27 induced by herbal formula astragali radix and rehmanniae radix
Journal of Ethnopharmacology, 2011Co-Authors: Qi Zhang, Chi Chun Fong, Yao Chen, Fan Wei, Chi Man Koon, Kit Man Lau, Pingchung Leung, Clara Biksan Lau, Kwokpui Fung, Mengsu YangAbstract:Abstract Ethnopharmacological relevance The herbs Astragali Radix (AR) and Rehmanniae Radix (RR) have long been used in traditional Chinese Medicine and serve as the principal herbs in treating diabetic foot ulcer. Aim of the study Chinese herbal formulus comprising Astragali Radix (AR) and Rehmanniae Radix (RR) have been shown to improve the healing of diabetic foot ulcer through enhancing the viability of primary Fibroblasts in diabetic patients suffering insulin resistance. Our previous study demonstrated that the herbal formula NF3 comprising of AR and RR in the ratio of 2:1 was effective in promoting wound healing in diabetic rats, and in vitro data indicated that the wound healing effects of NF3 might be due to the regulation and coordination of inflammation, angiogenesis and tissue regeneration. However, the underlying molecular mechanism has not been well investigated. In this study, we investigated the Cellular and molecular effects of the herbal formula NF3 on human skin Fibroblast Cells. Materials and methods Human skin Fibroblast Cells Hs27 were treated with NF3 ranging from 0 to 8 mg/ml for 24 h, and the Cells without NF3 treatment were used as control. Cell proliferation assay and Cell cycle analysis were performed. Transcriptional profiles of Hs27 Cells upon NF3 treatment were acquired by using a human cDNA microarray containing 10,000 genes, and the signaling pathways differentially regulated by NF3 were identified and analyzed. Results NF3 promoted Hs27 Cell proliferation and Cell cycle progression. Microarray analysis revealed that 116 genes were differentially expressed upon NF3 treatment. Functional analysis of the genes indicated that NF3 mainly activated Wnt and angiogenesis related pathways, which are directly related to Cell proliferation, angiogenesis, extraCellular matrix (ECM) formation and inflammation during the process of wound healing. Conclusion This study provides insight into the molecular mechanism of how the herbal formula Astragali Radix and Rehmanniae Radix may serve as potential therapeutics for wound healing.