The Experts below are selected from a list of 240 Experts worldwide ranked by ideXlab platform

Alexander J Lazar - One of the best experts on this subject based on the ideXlab platform.

  • fus rearrangements are rare in pure sclerosing epithelioid Fibrosarcoma
    Modern Pathology, 2012
    Co-Authors: Wei Lien Wang, Brian P Rubin, Harry L Evans, Jeanne M Meis, Bernadette Lieglatzwanger, Judith V M G Bovee, John R Goldblum, Steven D Billings, Dolores Lopezterrada, Alexander J Lazar
    Abstract:

    Several recent reports have described low-grade fibromyxoid sarcoma with sclerosing epithelioid Fibrosarcoma-like areas. We evaluated cases of pure sclerosing epithelioid Fibrosarcoma lacking areas of low-grade fibromyxoid sarcoma for FUS rearrangement to determine whether this entity could be related to low-grade fibromyxoid sarcoma. Available formalin-fixed paraffin-embedded tissue of 27 sclerosing epithelioid Fibrosarcoma from 25 patients was retrieved and tabulated with clinical information. Unstained slides from formalin-fixed paraffin-embedded blocks were prepared and fluorescence in-situ hybridization was performed using a commercial FUS break-apart probe. The median patient age at presentation was 50 (range, 14–78) years, with 14 males and 10 females. Sclerosing epithelioid Fibrosarcoma most commonly involved the extremities (n=8) or chest (n=6). Sixteen patients had a median follow-up of 17 (range, 1–99) months; seven were alive and well at 12 (range, 5–30) months; three alive with disease at 28 (range, 9–99) months; five dead of disease at a median of 22 (range, 1–36) months and one was dead of unknown causes. Twelve patients were known to have metastases; the most common site was lung (n=7), followed by bone (n=3), lymph nodes (n=2) and peritoneum (n=1). Only 2 of 22 (9%) analyzable cases of sclerosing epithelioid Fibrosarcoma showed rearrangement in the FUS locus by fluorescence in-situ hybridization. Although cytogenetically confirmed low-grade fibromyxoid sarcoma can have sclerosing epithelioid Fibrosarcoma-like areas, FUS rearrangement, which is characteristic of low-grade fibromyxoid sarcoma, appears to be relatively rare in pure sclerosing epithelioid Fibrosarcoma.

Pierpaolo Aimola - One of the best experts on this subject based on the ideXlab platform.

  • targeting a newly established spontaneous feline Fibrosarcoma cell line by gene transfer
    PLOS ONE, 2012
    Co-Authors: Rounak Nande, Altomare Di Benedetto, Pierpaolo Aimola, Flavia De Carlo, Miranda B Carper
    Abstract:

    Fibrosarcoma is a deadly disease in cats and is significantly more often located at classical vaccine injections sites. More rare forms of spontaneous non-vaccination site (NSV) Fibrosarcomas have been described and have been found associated to genetic alterations. Purpose of this study was to compare the efficacy of adenoviral gene transfer in NVS Fibrosarcoma. We isolated and characterized a NVS Fibrosarcoma cell line (Cocca-6A) from a spontaneous Fibrosarcoma that occurred in a domestic calico cat. The feline cells were karyotyped and their chromosome number was counted using a Giemsa staining. Adenoviral gene transfer was verified by western blot analysis. Flow cytometry assay and Annexin-V were used to study cell-cycle changes and cell death of transduced cells. Cocca-6A Fibrosarcoma cells were morphologically and cytogenetically characterized. Giemsa block staining of metaphase spreads of the Cocca-6A cells showed deletion of one of the E1 chromosomes, where feline p53 maps. Semi-quantitative PCR demonstrated reduction of p53 genomic DNA in the Cocca-6A cells. Adenoviral gene transfer determined a remarkable effect on the viability and growth of the Cocca-6A cells following single transduction with adenoviruses carrying Mda-7/IL-24 or IFN-γ or various combination of RB/p105, Ras-DN, IFN-γ, and Mda-7 gene transfer. Therapy for feline Fibrosarcomas is often insufficient for long lasting tumor eradication. More gene transfer studies should be conducted in order to understand if these viral vectors could be applicable regardless the origin (spontaneous vs. vaccine induced) of feline Fibrosarcomas.

Altomare Di Benedetto - One of the best experts on this subject based on the ideXlab platform.

  • targeting a newly established spontaneous feline Fibrosarcoma cell line by gene transfer
    PLOS ONE, 2012
    Co-Authors: Rounak Nande, Altomare Di Benedetto, Pierpaolo Aimola, Flavia De Carlo, Miranda B Carper
    Abstract:

    Fibrosarcoma is a deadly disease in cats and is significantly more often located at classical vaccine injections sites. More rare forms of spontaneous non-vaccination site (NSV) Fibrosarcomas have been described and have been found associated to genetic alterations. Purpose of this study was to compare the efficacy of adenoviral gene transfer in NVS Fibrosarcoma. We isolated and characterized a NVS Fibrosarcoma cell line (Cocca-6A) from a spontaneous Fibrosarcoma that occurred in a domestic calico cat. The feline cells were karyotyped and their chromosome number was counted using a Giemsa staining. Adenoviral gene transfer was verified by western blot analysis. Flow cytometry assay and Annexin-V were used to study cell-cycle changes and cell death of transduced cells. Cocca-6A Fibrosarcoma cells were morphologically and cytogenetically characterized. Giemsa block staining of metaphase spreads of the Cocca-6A cells showed deletion of one of the E1 chromosomes, where feline p53 maps. Semi-quantitative PCR demonstrated reduction of p53 genomic DNA in the Cocca-6A cells. Adenoviral gene transfer determined a remarkable effect on the viability and growth of the Cocca-6A cells following single transduction with adenoviruses carrying Mda-7/IL-24 or IFN-γ or various combination of RB/p105, Ras-DN, IFN-γ, and Mda-7 gene transfer. Therapy for feline Fibrosarcomas is often insufficient for long lasting tumor eradication. More gene transfer studies should be conducted in order to understand if these viral vectors could be applicable regardless the origin (spontaneous vs. vaccine induced) of feline Fibrosarcomas.

Lee So Maeng - One of the best experts on this subject based on the ideXlab platform.

  • A Case of Primary Ovarian Fibrosarcoma
    Obstetrics & gynecology science, 2006
    Co-Authors: Woo Mi Shin, Jae Yen Song, Yong Wook Kim, Tae Eung Kim, Jae Keun Jung, Lee So Maeng
    Abstract:

    Fibrosarcoma of the ovary is an exceedingly rare primary ovarian stromal tumor, which has a poor prognosis. Fibrosarcoma may arise de novo or as a result of malignant change in a benign fibromatous or fibrothecomatous tumor of the ovary. There are only about 30 cases in the literature reported within past three decades, showing the extreme rarity of these tumors. We report a case of primary ovarian Fibrosarcoma with a review of the available literature.

Brian P Rubin - One of the best experts on this subject based on the ideXlab platform.

  • fus rearrangements are rare in pure sclerosing epithelioid Fibrosarcoma
    Modern Pathology, 2012
    Co-Authors: Wei Lien Wang, Brian P Rubin, Harry L Evans, Jeanne M Meis, Bernadette Lieglatzwanger, Judith V M G Bovee, John R Goldblum, Steven D Billings, Dolores Lopezterrada, Alexander J Lazar
    Abstract:

    Several recent reports have described low-grade fibromyxoid sarcoma with sclerosing epithelioid Fibrosarcoma-like areas. We evaluated cases of pure sclerosing epithelioid Fibrosarcoma lacking areas of low-grade fibromyxoid sarcoma for FUS rearrangement to determine whether this entity could be related to low-grade fibromyxoid sarcoma. Available formalin-fixed paraffin-embedded tissue of 27 sclerosing epithelioid Fibrosarcoma from 25 patients was retrieved and tabulated with clinical information. Unstained slides from formalin-fixed paraffin-embedded blocks were prepared and fluorescence in-situ hybridization was performed using a commercial FUS break-apart probe. The median patient age at presentation was 50 (range, 14–78) years, with 14 males and 10 females. Sclerosing epithelioid Fibrosarcoma most commonly involved the extremities (n=8) or chest (n=6). Sixteen patients had a median follow-up of 17 (range, 1–99) months; seven were alive and well at 12 (range, 5–30) months; three alive with disease at 28 (range, 9–99) months; five dead of disease at a median of 22 (range, 1–36) months and one was dead of unknown causes. Twelve patients were known to have metastases; the most common site was lung (n=7), followed by bone (n=3), lymph nodes (n=2) and peritoneum (n=1). Only 2 of 22 (9%) analyzable cases of sclerosing epithelioid Fibrosarcoma showed rearrangement in the FUS locus by fluorescence in-situ hybridization. Although cytogenetically confirmed low-grade fibromyxoid sarcoma can have sclerosing epithelioid Fibrosarcoma-like areas, FUS rearrangement, which is characteristic of low-grade fibromyxoid sarcoma, appears to be relatively rare in pure sclerosing epithelioid Fibrosarcoma.

  • Neonate with a Fibrosarcoma and consumptive coagulopathy.
    Journal of the American Academy of Dermatology, 2004
    Co-Authors: Maryam Asgari, Brian P Rubin, Robin L Hornung
    Abstract:

    We report a case of a neonate with a congenital tumor on the left palm. The patient had a grapefruit-sized tumor and coagulopathy at birth and was presumed to have a hemangioendothelioma with associated Kasabach-Merritt phenomenon. When the tumor failed to respond to systemic steroids, a biopsy specimen was taken, revealing a Fibrosarcoma. We describe this case to highlight the importance of including rare tumors, such as Fibrosarcomas, in the clinical differential when evaluating a congenital tumor with associated coagulopathy. We discuss subtle differences in clinical presentations that might aid in differentiating vascular tumors from Fibrosarcomas.