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Na Wang - One of the best experts on this subject based on the ideXlab platform.
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Identification and analysis of the β-catenin1 gene in half-smooth tongue sole (Cynoglossus semilaevis).
PloS one, 2017Co-Authors: Ying Zhu, Changwei Shao, Hua Guo, Liang Meng, Min Wei, Na WangAbstract:β-catenin is a key signalling molecule in the canonical Wnt pathway, which plays a role in cell adhesion, embryogenesis and sex determination. However, little is known about its function in teleosts. We cloned and characterized the full-length β-catenin1 gene from half-smooth tongue sole (Cynoglossus semilaevis), which was designated CS-β-catenin1. The CS-β-catenin1 cDNA consists of 2,346 nucleotides and encodes a protein with 782 amino acids. Although CS-β-catenin1 was transcribed in the gonads of both sexes, the level was significantly higher in ovaries compared to testes. Furthermore, the mRNA level of CS-β-catenin1 was significantly upregulated at 160 days and constantly increased until 2 years of age. In situ hybridization revealed that CS-β-catenin1 mRNA was mainly localized in oocyte cells, especially in stage I, II and III oocytes. When CS-β-catenin1 expression was inhibited by injection of quercetin in the ovaries, levels of CS-FIGLA and CS-foxl2 mRNA were significantly down-regulated, and CS-dmrt1 was up-regulated, which suggested that CS-β-catenin1 is a potential upstream gene of CS-FIGLA and is involved in the development of the ovaries, i.e., folliculogenesis.
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Relative mRNA expression levels of CS-β-catenin1 and other sex-related genes after treatment with quercetin.
2017Co-Authors: Ying Zhu, Changwei Shao, Hua Guo, Liang Meng, Min Wei, Na WangAbstract:(A) Expression of CS-β-catenin1 24 and 48 h after injection with quercetin. (B) Expression of CS-β-catenin1, CS-FIGLA, CS-Foxl2, CS-Wnt4a and CS-dmrt1 48 h after injection with quercetin. The expression of the β-actin gene was used as an internal control. Three samples were handled. Values with asterisks indicate a significant difference (P
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Two FIGLA homologues have disparate functions during sex differentiation in half-smooth tongue sole (Cynoglossus semilaevis)
Scientific reports, 2016Co-Authors: Ning Zhang, Changwei Shao, Ying Zhu, Zhongdian Dong, Na Wang, Xiaodong Jia, Songlin ChenAbstract:FIGLA is a germ-cell-specific transcription factor associated with ovary development and differentiation. In vertebrates, one transcriptional form of FIGLA is commonly found. However, besides the common form of this gene (named FIGLA_tv1), a new transcriptional form (named FIGLA_tv2) was identified in half-smooth tongue sole (Cynoglossus semilaevis). The full-length cDNA of FIGLA_tv1 was 1057 bp long with a 591-bp open reading frame encoding a predicted 196 amino acid protein, while FIGLA_tv2 encoded a 125 amino acid protein. FIGLA_tv1 and FIGLA_tv2 expression in various tissues was detected by qRT-PCR. FIGLA_tv1 was expressed mainly in ovary, skin and liver, while FIGLA_tv2 was expressed in all examined tissues. In the gonads, FIGLA_tv1 was expressed in ovary, while FIGLA_tv2 was predominately expressed in testis of pseudomales. Further, in situ hybridization located FIGLA_tv1 only in oocytes and FIGLA_tv2 mainly in germ cells of pseudomale testis. After knocking down FIGLA_tv2 in a pseudomale testis cell line, the expression of two steroid hormone-encoding genes, StAR and P450scc, was significantly up-regulated (P
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two FIGLA homologues have disparate functions during sex differentiation in half smooth tongue sole cynoglossus semilaevis
Scientific Reports, 2016Co-Authors: Ning Zhang, Changwei Shao, Ying Zhu, Zhongdian Dong, Na Wang, Xiaodong Jia, Songlin ChenAbstract:FIGLA is a germ-cell-specific transcription factor associated with ovary development and differentiation. In vertebrates, one transcriptional form of FIGLA is commonly found. However, besides the common form of this gene (named FIGLA_tv1), a new transcriptional form (named FIGLA_tv2) was identified in half-smooth tongue sole (Cynoglossus semilaevis). The full-length cDNA of FIGLA_tv1 was 1057 bp long with a 591-bp open reading frame encoding a predicted 196 amino acid protein, while FIGLA_tv2 encoded a 125 amino acid protein. FIGLA_tv1 and FIGLA_tv2 expression in various tissues was detected by qRT-PCR. FIGLA_tv1 was expressed mainly in ovary, skin and liver, while FIGLA_tv2 was expressed in all examined tissues. In the gonads, FIGLA_tv1 was expressed in ovary, while FIGLA_tv2 was predominately expressed in testis of pseudomales. Further, in situ hybridization located FIGLA_tv1 only in oocytes and FIGLA_tv2 mainly in germ cells of pseudomale testis. After knocking down FIGLA_tv2 in a pseudomale testis cell line, the expression of two steroid hormone-encoding genes, StAR and P450scc, was significantly up-regulated (P < 0.05). Our findings suggest that FIGLA_tv1 has a conserved function in folliculogenesis, as in other vertebrates, and that FIGLA_tv2 may have a role in the spermatogenesis of pseudomales by regulating the synthesis and metabolism of steroid hormones.
Songlin Chen - One of the best experts on this subject based on the ideXlab platform.
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Two FIGLA homologues have disparate functions during sex differentiation in half-smooth tongue sole (Cynoglossus semilaevis)
Scientific reports, 2016Co-Authors: Ning Zhang, Changwei Shao, Ying Zhu, Zhongdian Dong, Na Wang, Xiaodong Jia, Songlin ChenAbstract:FIGLA is a germ-cell-specific transcription factor associated with ovary development and differentiation. In vertebrates, one transcriptional form of FIGLA is commonly found. However, besides the common form of this gene (named FIGLA_tv1), a new transcriptional form (named FIGLA_tv2) was identified in half-smooth tongue sole (Cynoglossus semilaevis). The full-length cDNA of FIGLA_tv1 was 1057 bp long with a 591-bp open reading frame encoding a predicted 196 amino acid protein, while FIGLA_tv2 encoded a 125 amino acid protein. FIGLA_tv1 and FIGLA_tv2 expression in various tissues was detected by qRT-PCR. FIGLA_tv1 was expressed mainly in ovary, skin and liver, while FIGLA_tv2 was expressed in all examined tissues. In the gonads, FIGLA_tv1 was expressed in ovary, while FIGLA_tv2 was predominately expressed in testis of pseudomales. Further, in situ hybridization located FIGLA_tv1 only in oocytes and FIGLA_tv2 mainly in germ cells of pseudomale testis. After knocking down FIGLA_tv2 in a pseudomale testis cell line, the expression of two steroid hormone-encoding genes, StAR and P450scc, was significantly up-regulated (P
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two FIGLA homologues have disparate functions during sex differentiation in half smooth tongue sole cynoglossus semilaevis
Scientific Reports, 2016Co-Authors: Ning Zhang, Changwei Shao, Ying Zhu, Zhongdian Dong, Na Wang, Xiaodong Jia, Songlin ChenAbstract:FIGLA is a germ-cell-specific transcription factor associated with ovary development and differentiation. In vertebrates, one transcriptional form of FIGLA is commonly found. However, besides the common form of this gene (named FIGLA_tv1), a new transcriptional form (named FIGLA_tv2) was identified in half-smooth tongue sole (Cynoglossus semilaevis). The full-length cDNA of FIGLA_tv1 was 1057 bp long with a 591-bp open reading frame encoding a predicted 196 amino acid protein, while FIGLA_tv2 encoded a 125 amino acid protein. FIGLA_tv1 and FIGLA_tv2 expression in various tissues was detected by qRT-PCR. FIGLA_tv1 was expressed mainly in ovary, skin and liver, while FIGLA_tv2 was expressed in all examined tissues. In the gonads, FIGLA_tv1 was expressed in ovary, while FIGLA_tv2 was predominately expressed in testis of pseudomales. Further, in situ hybridization located FIGLA_tv1 only in oocytes and FIGLA_tv2 mainly in germ cells of pseudomale testis. After knocking down FIGLA_tv2 in a pseudomale testis cell line, the expression of two steroid hormone-encoding genes, StAR and P450scc, was significantly up-regulated (P < 0.05). Our findings suggest that FIGLA_tv1 has a conserved function in folliculogenesis, as in other vertebrates, and that FIGLA_tv2 may have a role in the spermatogenesis of pseudomales by regulating the synthesis and metabolism of steroid hormones.
Linyan Zhou - One of the best experts on this subject based on the ideXlab platform.
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RESEARCH ARTICLE FIGLA Favors Ovarian Differentiation by Antagonizing Spermatogenesis in a Teleosts, Nile Tilapia (Oreochromis niloticus)
2016Co-Authors: Yongxiu Qiu, Shaohua Sun, Tapas Charkraborty, Lina Sun, Yoshitaka Nagahama, Linyan ZhouAbstract:FIGLA (factor in the germ line, alpha), a female germ cell-specific transcription factor, had been shown to activate genetic hierarchies in oocytes. The ectopic expression of FIGLA was known to repress spermatogenesis-associated genes in male mice. However, the potential role of FIGLA in other vertebrates remains elusive. The present work was aimed to identify and characterize the functional relevance of FIGLA in the ovarian development of Nile tilapia (Oreochromis niloticus). Tissue distribution and ontogeny analysis revealed that tilapia FIGLA gene was dominantly expressed in the ovary from 30 days after hatching. Immunohis-tochemistry analysis also demonstrated that FIGLA was expressed in the cytoplasm of early primary oocytes. Intriguingly, over-expression of FIGLA in XY fish resulted in the disruption of spermatogenesis along with the depletion of meiotic spermatocytes and spermatids in tes-tis. Dramatic decline of sycp3 (synaptonemal complex protein 3) and prm (protamine) ex-pression indicates that meiotic spermatocytes and mature sperm production are impaired. Even though Sertoli cell (dmrt1) and Leydig cell (star and cyp17a1) marker genes remained unaffected, hsd3b1 expression and 11-KT production were enhanced in FIGLA-transgene testis. Taken together, our data suggest that fish FIGLAmight play an essential role in the ovarian development by antagonizing spermatogenesis
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FIGLA favors ovarian differentiation by antagonizing spermatogenesis in a teleosts, Nile tilapia (Oreochromis niloticus)
PloS one, 2015Co-Authors: Yongxiu Qiu, Shaohua Sun, Tapas Charkraborty, Lina Sun, Jing Wei, Yoshitaka Nagahama, Deshou Wang, Linyan ZhouAbstract:FIGLA (factor in the germ line, alpha), a female germ cell-specific transcription factor, had been shown to activate genetic hierarchies in oocytes. The ectopic expression of FIGLA was known to repress spermatogenesis-associated genes in male mice. However, the potential role of FIGLA in other vertebrates remains elusive. The present work was aimed to identify and characterize the functional relevance of FIGLA in the ovarian development of Nile tilapia (Oreochromis niloticus). Tissue distribution and ontogeny analysis revealed that tilapia FIGLA gene was dominantly expressed in the ovary from 30 days after hatching. Immunohistochemistry analysis also demonstrated that FIGLA was expressed in the cytoplasm of early primary oocytes. Intriguingly, over-expression of FIGLA in XY fish resulted in the disruption of spermatogenesis along with the depletion of meiotic spermatocytes and spermatids in testis. Dramatic decline of sycp3 (synaptonemal complex protein 3) and prm (protamine) expression indicates that meiotic spermatocytes and mature sperm production are impaired. Even though Sertoli cell (dmrt1) and Leydig cell (star and cyp17a1) marker genes remained unaffected, hsd3b1 expression and 11-KT production were enhanced in FIGLA-transgene testis. Taken together, our data suggest that fish FIGLA might play an essential role in the ovarian development by antagonizing spermatogenesis.
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Screening, analysis and histological investigations of Fig1a-transgene fish.
2015Co-Authors: Yongxiu Qiu, Shaohua Sun, Tapas Charkraborty, Lina Sun, Jing Wei, Yoshitaka Nagahama, Deshou Wang, Linyan ZhouAbstract:(A) Confirmation of transgene insertion by genomic PCR and sequencing. Lane 1 and 6, DNA marker; Lane 2, XY fish carrying FIGLA-transgene; Lane 3 and 4, using plasmid and water as template, respectively; Lane 5, XY control fish. (B) Comparison of GSI (Gonadal Somatic Index) of control and Fig1a-transgene XY fish. Data were expressed as means ± S.E.M. of control (n = 4) and Fig1a-transgene XY (n = 13) fish. Different letters indicate statistical differences at P
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Effects of Fig1a over-expression on subcellular re-organization of somatic and germ cell markers.
2015Co-Authors: Yongxiu Qiu, Shaohua Sun, Tapas Charkraborty, Lina Sun, Jing Wei, Yoshitaka Nagahama, Deshou Wang, Linyan ZhouAbstract:No significant difference of Dmrt1, Cyp17a1 and StAR were detected between Fig1a-transgene (A, C, E) and control group (B, D, F). Abundant expression of Sycp3 was detected in the testis of the control fish (H), while no positive immunostaining for Sycp3 was detected in the testis for the FIGLA-transgene fish (G). Arrows indicate positive immunostaining for each gene.
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Expression profile of FIGLA gene in tilapia.
2015Co-Authors: Yongxiu Qiu, Shaohua Sun, Tapas Charkraborty, Lina Sun, Jing Wei, Yoshitaka Nagahama, Deshou Wang, Linyan ZhouAbstract:(A) Expression of FIGLA in adult tilapia tissues by real-time PCR. HK, head kidney; M, muscle; K, kidney; O, ovary; B, brain; T, testis; G, gill; H, heart; L, liver; I, intestine; S, spleen. (B) Ontogenic expression of FIGLA gene in tilapia gonads analyzed by real-time PCR. Data were represented as means ± S.E.M. of three independent samples. The expression level was normalized using the geometric mean of the levels of three internal control genes (gapdh, ef1a, and actb). Different lower-case letters indicate a significant difference in FIGLA mRNA levels (P
Ying Zhu - One of the best experts on this subject based on the ideXlab platform.
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Identification and analysis of the β-catenin1 gene in half-smooth tongue sole (Cynoglossus semilaevis).
PloS one, 2017Co-Authors: Ying Zhu, Changwei Shao, Hua Guo, Liang Meng, Min Wei, Na WangAbstract:β-catenin is a key signalling molecule in the canonical Wnt pathway, which plays a role in cell adhesion, embryogenesis and sex determination. However, little is known about its function in teleosts. We cloned and characterized the full-length β-catenin1 gene from half-smooth tongue sole (Cynoglossus semilaevis), which was designated CS-β-catenin1. The CS-β-catenin1 cDNA consists of 2,346 nucleotides and encodes a protein with 782 amino acids. Although CS-β-catenin1 was transcribed in the gonads of both sexes, the level was significantly higher in ovaries compared to testes. Furthermore, the mRNA level of CS-β-catenin1 was significantly upregulated at 160 days and constantly increased until 2 years of age. In situ hybridization revealed that CS-β-catenin1 mRNA was mainly localized in oocyte cells, especially in stage I, II and III oocytes. When CS-β-catenin1 expression was inhibited by injection of quercetin in the ovaries, levels of CS-FIGLA and CS-foxl2 mRNA were significantly down-regulated, and CS-dmrt1 was up-regulated, which suggested that CS-β-catenin1 is a potential upstream gene of CS-FIGLA and is involved in the development of the ovaries, i.e., folliculogenesis.
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Relative mRNA expression levels of CS-β-catenin1 and other sex-related genes after treatment with quercetin.
2017Co-Authors: Ying Zhu, Changwei Shao, Hua Guo, Liang Meng, Min Wei, Na WangAbstract:(A) Expression of CS-β-catenin1 24 and 48 h after injection with quercetin. (B) Expression of CS-β-catenin1, CS-FIGLA, CS-Foxl2, CS-Wnt4a and CS-dmrt1 48 h after injection with quercetin. The expression of the β-actin gene was used as an internal control. Three samples were handled. Values with asterisks indicate a significant difference (P
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Two FIGLA homologues have disparate functions during sex differentiation in half-smooth tongue sole (Cynoglossus semilaevis)
Scientific reports, 2016Co-Authors: Ning Zhang, Changwei Shao, Ying Zhu, Zhongdian Dong, Na Wang, Xiaodong Jia, Songlin ChenAbstract:FIGLA is a germ-cell-specific transcription factor associated with ovary development and differentiation. In vertebrates, one transcriptional form of FIGLA is commonly found. However, besides the common form of this gene (named FIGLA_tv1), a new transcriptional form (named FIGLA_tv2) was identified in half-smooth tongue sole (Cynoglossus semilaevis). The full-length cDNA of FIGLA_tv1 was 1057 bp long with a 591-bp open reading frame encoding a predicted 196 amino acid protein, while FIGLA_tv2 encoded a 125 amino acid protein. FIGLA_tv1 and FIGLA_tv2 expression in various tissues was detected by qRT-PCR. FIGLA_tv1 was expressed mainly in ovary, skin and liver, while FIGLA_tv2 was expressed in all examined tissues. In the gonads, FIGLA_tv1 was expressed in ovary, while FIGLA_tv2 was predominately expressed in testis of pseudomales. Further, in situ hybridization located FIGLA_tv1 only in oocytes and FIGLA_tv2 mainly in germ cells of pseudomale testis. After knocking down FIGLA_tv2 in a pseudomale testis cell line, the expression of two steroid hormone-encoding genes, StAR and P450scc, was significantly up-regulated (P
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two FIGLA homologues have disparate functions during sex differentiation in half smooth tongue sole cynoglossus semilaevis
Scientific Reports, 2016Co-Authors: Ning Zhang, Changwei Shao, Ying Zhu, Zhongdian Dong, Na Wang, Xiaodong Jia, Songlin ChenAbstract:FIGLA is a germ-cell-specific transcription factor associated with ovary development and differentiation. In vertebrates, one transcriptional form of FIGLA is commonly found. However, besides the common form of this gene (named FIGLA_tv1), a new transcriptional form (named FIGLA_tv2) was identified in half-smooth tongue sole (Cynoglossus semilaevis). The full-length cDNA of FIGLA_tv1 was 1057 bp long with a 591-bp open reading frame encoding a predicted 196 amino acid protein, while FIGLA_tv2 encoded a 125 amino acid protein. FIGLA_tv1 and FIGLA_tv2 expression in various tissues was detected by qRT-PCR. FIGLA_tv1 was expressed mainly in ovary, skin and liver, while FIGLA_tv2 was expressed in all examined tissues. In the gonads, FIGLA_tv1 was expressed in ovary, while FIGLA_tv2 was predominately expressed in testis of pseudomales. Further, in situ hybridization located FIGLA_tv1 only in oocytes and FIGLA_tv2 mainly in germ cells of pseudomale testis. After knocking down FIGLA_tv2 in a pseudomale testis cell line, the expression of two steroid hormone-encoding genes, StAR and P450scc, was significantly up-regulated (P < 0.05). Our findings suggest that FIGLA_tv1 has a conserved function in folliculogenesis, as in other vertebrates, and that FIGLA_tv2 may have a role in the spermatogenesis of pseudomales by regulating the synthesis and metabolism of steroid hormones.
Changwei Shao - One of the best experts on this subject based on the ideXlab platform.
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Identification and analysis of the β-catenin1 gene in half-smooth tongue sole (Cynoglossus semilaevis).
PloS one, 2017Co-Authors: Ying Zhu, Changwei Shao, Hua Guo, Liang Meng, Min Wei, Na WangAbstract:β-catenin is a key signalling molecule in the canonical Wnt pathway, which plays a role in cell adhesion, embryogenesis and sex determination. However, little is known about its function in teleosts. We cloned and characterized the full-length β-catenin1 gene from half-smooth tongue sole (Cynoglossus semilaevis), which was designated CS-β-catenin1. The CS-β-catenin1 cDNA consists of 2,346 nucleotides and encodes a protein with 782 amino acids. Although CS-β-catenin1 was transcribed in the gonads of both sexes, the level was significantly higher in ovaries compared to testes. Furthermore, the mRNA level of CS-β-catenin1 was significantly upregulated at 160 days and constantly increased until 2 years of age. In situ hybridization revealed that CS-β-catenin1 mRNA was mainly localized in oocyte cells, especially in stage I, II and III oocytes. When CS-β-catenin1 expression was inhibited by injection of quercetin in the ovaries, levels of CS-FIGLA and CS-foxl2 mRNA were significantly down-regulated, and CS-dmrt1 was up-regulated, which suggested that CS-β-catenin1 is a potential upstream gene of CS-FIGLA and is involved in the development of the ovaries, i.e., folliculogenesis.
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Relative mRNA expression levels of CS-β-catenin1 and other sex-related genes after treatment with quercetin.
2017Co-Authors: Ying Zhu, Changwei Shao, Hua Guo, Liang Meng, Min Wei, Na WangAbstract:(A) Expression of CS-β-catenin1 24 and 48 h after injection with quercetin. (B) Expression of CS-β-catenin1, CS-FIGLA, CS-Foxl2, CS-Wnt4a and CS-dmrt1 48 h after injection with quercetin. The expression of the β-actin gene was used as an internal control. Three samples were handled. Values with asterisks indicate a significant difference (P
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Two FIGLA homologues have disparate functions during sex differentiation in half-smooth tongue sole (Cynoglossus semilaevis)
Scientific reports, 2016Co-Authors: Ning Zhang, Changwei Shao, Ying Zhu, Zhongdian Dong, Na Wang, Xiaodong Jia, Songlin ChenAbstract:FIGLA is a germ-cell-specific transcription factor associated with ovary development and differentiation. In vertebrates, one transcriptional form of FIGLA is commonly found. However, besides the common form of this gene (named FIGLA_tv1), a new transcriptional form (named FIGLA_tv2) was identified in half-smooth tongue sole (Cynoglossus semilaevis). The full-length cDNA of FIGLA_tv1 was 1057 bp long with a 591-bp open reading frame encoding a predicted 196 amino acid protein, while FIGLA_tv2 encoded a 125 amino acid protein. FIGLA_tv1 and FIGLA_tv2 expression in various tissues was detected by qRT-PCR. FIGLA_tv1 was expressed mainly in ovary, skin and liver, while FIGLA_tv2 was expressed in all examined tissues. In the gonads, FIGLA_tv1 was expressed in ovary, while FIGLA_tv2 was predominately expressed in testis of pseudomales. Further, in situ hybridization located FIGLA_tv1 only in oocytes and FIGLA_tv2 mainly in germ cells of pseudomale testis. After knocking down FIGLA_tv2 in a pseudomale testis cell line, the expression of two steroid hormone-encoding genes, StAR and P450scc, was significantly up-regulated (P
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two FIGLA homologues have disparate functions during sex differentiation in half smooth tongue sole cynoglossus semilaevis
Scientific Reports, 2016Co-Authors: Ning Zhang, Changwei Shao, Ying Zhu, Zhongdian Dong, Na Wang, Xiaodong Jia, Songlin ChenAbstract:FIGLA is a germ-cell-specific transcription factor associated with ovary development and differentiation. In vertebrates, one transcriptional form of FIGLA is commonly found. However, besides the common form of this gene (named FIGLA_tv1), a new transcriptional form (named FIGLA_tv2) was identified in half-smooth tongue sole (Cynoglossus semilaevis). The full-length cDNA of FIGLA_tv1 was 1057 bp long with a 591-bp open reading frame encoding a predicted 196 amino acid protein, while FIGLA_tv2 encoded a 125 amino acid protein. FIGLA_tv1 and FIGLA_tv2 expression in various tissues was detected by qRT-PCR. FIGLA_tv1 was expressed mainly in ovary, skin and liver, while FIGLA_tv2 was expressed in all examined tissues. In the gonads, FIGLA_tv1 was expressed in ovary, while FIGLA_tv2 was predominately expressed in testis of pseudomales. Further, in situ hybridization located FIGLA_tv1 only in oocytes and FIGLA_tv2 mainly in germ cells of pseudomale testis. After knocking down FIGLA_tv2 in a pseudomale testis cell line, the expression of two steroid hormone-encoding genes, StAR and P450scc, was significantly up-regulated (P < 0.05). Our findings suggest that FIGLA_tv1 has a conserved function in folliculogenesis, as in other vertebrates, and that FIGLA_tv2 may have a role in the spermatogenesis of pseudomales by regulating the synthesis and metabolism of steroid hormones.