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Jianping Chen - One of the best experts on this subject based on the ideXlab platform.
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Phloem-limited reoviruses universally induce sieve element hyperplasia and more flexible gateways, providing more channels for their movement in plants.
Scientific Reports, 2017Co-Authors: Li Xie, Jianping Chen, Xi-jiao Song, Jian Hong, Qianzhuo Mao, Heng-mu ZhangAbstract:Virion distribution and ultrastructural changes induced by the infection of maize or rice with four different reoviruses were examined. Rice black streaked dwarf virus (RBSDV, genus Fijivirus), Rice ragged stunt virus (RRSV, genus Oryzavirus), and Rice gall dwarf virus (RGDV, genus Phytoreovirus) were all phloem-limited and caused cellular hyperplasia in the phloem resulting in tumors or vein swelling and modifying the cellular arrangement of sieve elements (SEs). In contrast, virions of Rice dwarf virus (RDV, genus Phytoreovirus) were observed in both phloem and mesophyll and the virus did not cause hyperplasia of SEs. The three phloem-limited reoviruses (but not RDV) all induced more flexible gateways at the SE-SE interfaces, especially the non-sieve plate interfaces. These flexible gateways were also observed for the first time at the cellular interfaces between SE and phloem parenchyma (PP). In plants infected with any of the reoviruses, virus-like particles could be seen within the flexible gateways, suggesting that these gateways may serve as channels for the movement of plant reoviruses with their large virions between SEs or between SEs and PP. SE hyperplasia and the increase in flexible gateways may be a universal strategy for the movement of phloem-limited reoviruses.
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biology of southern rice black streaked dwarf virus a novel Fijivirus emerging in east asia
Plant Pathology, 2017Co-Authors: Li Xie, Jianping Chen, Hua-di Wang, Huifu Wang, Heng-mu ZhangAbstract:Southern rice black-streaked dwarf virus (SRBSDV) was first reported in southern China in 2001 and causes a striking disease on rice and maize that leads to serious yield losses in several East Asian countries, such as China, Vietnam and Japan. A large research effort has been directed to understanding the virus and controlling the disease. Its geographic distribution, disease cycle via its insect vector, genome organization, relationship with host plants, and epidemiology are summarised in this review and the important role played by the vector, the white-backed planthopper (Sogatella furcifera Horvath), is emphasised. Countermeasures to control the disease that have been developed and applied include molecular detection for precise forecasting, chemical, physical, and ecological pest management. There is widespread insecticide resistance in the vector population but it is hoped that current efforts to develop rice cultivars resistant to the virus will eventually provide effective and cost-effective control. This article is protected by copyright. All rights reserved.
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interaction between southern rice black streaked dwarf virus minor core protein p8 and a rice zinc finger transcription factor
Archives of Virology, 2017Co-Authors: Nianjun Cai, Jian Yang, Jianping Chen, Jin Xue, Heng-mu ZhangAbstract:The Fijivirus southern rice black-streaked dwarf virus (SRBSDV) causes one of the most serious viral diseases of rice in China and Vietnam. To better understand the molecular basis of SRBSDV infection, a yeast two-hybrid screen of a rice cDNA library was carried out using P8, a minor core protein of SRBSDV, as the bait. A rice Cys2His2-type zinc finger protein (OsZFP) was found to interact with SRBSDV P8. A strong interaction between SRBSDV P8 and OsZFP was then confirmed by pull-down assays, and bimolecular fluorescence complementation assays showed that the in vivo interaction was specifically localized in the nucleus of plant cells. Using a series of deletion mutants, it was shown that both the NTP-binding region of P8 and the first two zinc fingers of OsZFP were crucial for their interaction in plant cells. The localization in the nucleus and activation of transcription in yeast supports the notion that OsZFP is a transcription factor. SRBSDV P8 may play an important role in fijiviral infection and symptom development by interfering with the host transcription activity of OsZFP.
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Characterization of Rice Black-Streaked Dwarf Virus- and Rice Stripe Virus-Derived siRNAs in Singly and Doubly Infected Insect Vector Laodelphax striatellus
2016Co-Authors: Ida Bagus Andika, Jiangfeng Shen, Liying Sun, Jianping ChenAbstract:Replication of RNA viruses in insect cells triggers an antiviral defense that is mediated by RNA interference (RNAi) which generates viral-derived small interfering RNAs (siRNAs). However, it is not known whether an antiviral RNAi response is also induced in insects by reoviruses, whose double-stranded RNA genome replication is thought to occur within core particles. Deep sequencing of small RNAs showed that when the small brown planthopper (Laodelphax striatellus) was infected by Rice black-streaked dwarf virus (RBSDV) (Reoviridae; Fijivirus), more viral-derived siRNAs accumulated than when the vector insect was infected by Rice stripe virus (RSV), a negative single-stranded RNA virus. RBSDV siRNAs were predominantly 21 and 22 nucleotides long and there were almost equal numbers of positive and negative sense. RBSDV siRNAs were frequently generated from hotspots in the 59- and 39-terminal regions of viral genome segments but these hotspots were not associated with any predicted RNA secondary structures. Under laboratory condition, L. striatellus can be infected simultaneously with RBSDV and RSV. Double infection enhanced the accumulation of particular genome segments but not viral coat protein of RBSDV and correlated with an increase in the abundance of siRNAs derived from RBSDV. The results o
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complete genomic sequence of maize rough dwarf virus a Fijivirus transmitted by the small brown planthopper
Genome Announcements, 2016Co-Authors: Li Xie, Jian Yang, Jianping Chen, Heng-mu ZhangAbstract:The nucleotide sequences of the 10 genomic segments of an Italian isolate of maize rough dwarf virus (MRDV) were determined. This first complete genomic sequence of MRDV will help understand the phylogenetic relationships among group 2 Fijiviruses and especially the closely related rice black-streaked dwarf virus, which is also found to naturally infect maize.
Heng-mu Zhang - One of the best experts on this subject based on the ideXlab platform.
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Phloem-limited reoviruses universally induce sieve element hyperplasia and more flexible gateways, providing more channels for their movement in plants.
Scientific Reports, 2017Co-Authors: Li Xie, Jianping Chen, Xi-jiao Song, Jian Hong, Qianzhuo Mao, Heng-mu ZhangAbstract:Virion distribution and ultrastructural changes induced by the infection of maize or rice with four different reoviruses were examined. Rice black streaked dwarf virus (RBSDV, genus Fijivirus), Rice ragged stunt virus (RRSV, genus Oryzavirus), and Rice gall dwarf virus (RGDV, genus Phytoreovirus) were all phloem-limited and caused cellular hyperplasia in the phloem resulting in tumors or vein swelling and modifying the cellular arrangement of sieve elements (SEs). In contrast, virions of Rice dwarf virus (RDV, genus Phytoreovirus) were observed in both phloem and mesophyll and the virus did not cause hyperplasia of SEs. The three phloem-limited reoviruses (but not RDV) all induced more flexible gateways at the SE-SE interfaces, especially the non-sieve plate interfaces. These flexible gateways were also observed for the first time at the cellular interfaces between SE and phloem parenchyma (PP). In plants infected with any of the reoviruses, virus-like particles could be seen within the flexible gateways, suggesting that these gateways may serve as channels for the movement of plant reoviruses with their large virions between SEs or between SEs and PP. SE hyperplasia and the increase in flexible gateways may be a universal strategy for the movement of phloem-limited reoviruses.
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biology of southern rice black streaked dwarf virus a novel Fijivirus emerging in east asia
Plant Pathology, 2017Co-Authors: Li Xie, Jianping Chen, Hua-di Wang, Huifu Wang, Heng-mu ZhangAbstract:Southern rice black-streaked dwarf virus (SRBSDV) was first reported in southern China in 2001 and causes a striking disease on rice and maize that leads to serious yield losses in several East Asian countries, such as China, Vietnam and Japan. A large research effort has been directed to understanding the virus and controlling the disease. Its geographic distribution, disease cycle via its insect vector, genome organization, relationship with host plants, and epidemiology are summarised in this review and the important role played by the vector, the white-backed planthopper (Sogatella furcifera Horvath), is emphasised. Countermeasures to control the disease that have been developed and applied include molecular detection for precise forecasting, chemical, physical, and ecological pest management. There is widespread insecticide resistance in the vector population but it is hoped that current efforts to develop rice cultivars resistant to the virus will eventually provide effective and cost-effective control. This article is protected by copyright. All rights reserved.
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interaction between southern rice black streaked dwarf virus minor core protein p8 and a rice zinc finger transcription factor
Archives of Virology, 2017Co-Authors: Nianjun Cai, Jian Yang, Jianping Chen, Jin Xue, Heng-mu ZhangAbstract:The Fijivirus southern rice black-streaked dwarf virus (SRBSDV) causes one of the most serious viral diseases of rice in China and Vietnam. To better understand the molecular basis of SRBSDV infection, a yeast two-hybrid screen of a rice cDNA library was carried out using P8, a minor core protein of SRBSDV, as the bait. A rice Cys2His2-type zinc finger protein (OsZFP) was found to interact with SRBSDV P8. A strong interaction between SRBSDV P8 and OsZFP was then confirmed by pull-down assays, and bimolecular fluorescence complementation assays showed that the in vivo interaction was specifically localized in the nucleus of plant cells. Using a series of deletion mutants, it was shown that both the NTP-binding region of P8 and the first two zinc fingers of OsZFP were crucial for their interaction in plant cells. The localization in the nucleus and activation of transcription in yeast supports the notion that OsZFP is a transcription factor. SRBSDV P8 may play an important role in fijiviral infection and symptom development by interfering with the host transcription activity of OsZFP.
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complete genomic sequence of maize rough dwarf virus a Fijivirus transmitted by the small brown planthopper
Genome Announcements, 2016Co-Authors: Li Xie, Jian Yang, Jianping Chen, Heng-mu ZhangAbstract:The nucleotide sequences of the 10 genomic segments of an Italian isolate of maize rough dwarf virus (MRDV) were determined. This first complete genomic sequence of MRDV will help understand the phylogenetic relationships among group 2 Fijiviruses and especially the closely related rice black-streaked dwarf virus, which is also found to naturally infect maize.
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p5 2 of rice black streaked dwarf virus is a non structural protein targeted to chloroplasts
Archives of Virology, 2015Co-Authors: Xiaoya Liu, Jianping Chen, Jian Yang, Li Xie, Xi-jiao Song, Heng-mu ZhangAbstract:The genome segment S5 of rice black-streaked dwarf virus (genus Fijivirus, family Reoviridae) is functionally bicistronic in infected plants. It has a conserved second ORF (P5-2) partially overlapping the major ORF in a different reading frame, but its function remains unknown. P5-2 was detected in infected plants, but not in purified viral particles by Western blotting, indicating that it is a non-structural protein. In immunoelectron microscopy, polyclonal antibodies against P5-2 specifically labelled chloroplasts of infected rice plants. When P5-2 fused with green fluorescent protein was transiently expressed in leaves of Nicotiana benthamiana, fluorescence was also co-localized with chloroplasts. Experiments with deletion mutants of P5-2 showed that its N-terminal part was responsible for its targeting to chloroplasts.
M Del Vas - One of the best experts on this subject based on the ideXlab platform.
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Reference gene selection for gene expression studies using RT-qPCR in virus-infected planthoppers
Virology Journal, 2011Co-Authors: Guillermo A Maroniche, Vanesa C Mongelli, Mónica Sagadín, Graciela A Truol, M Del VasAbstract:Background Planthoppers not only severely affect crops by causing mechanical damage when feeding but are also vectors of several plant virus species. The analysis of gene expression in persistently infected planthoppers might unveil the molecular basis of viral transmission. Quantitative real-time RT-PCR (RT-qPCR) is currently the most accurate and sensitive method used for quantitative gene expression analysis. In order to normalize the resulting quantitative data, reference genes with constant expression during the experimental procedures are needed. Results Partial sequences of the commonly used reference genes actin (ACT), α1-tubulin (TUB), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), elongation factor 1 alpha (EF1A), ribosomal protein S18 (RPS18) and polyubiquitin C (UBI) from Delphacodes kuscheli , a planthopper capable of persistently transmitting the plant Fijivirus Mal de Río Cuarto virus (MRCV), were isolated for the first time. Specific RT-qPCR primers were designed and the expression stability of these genes was assayed in MRCV-infective and naïve planthoppers using geNorm, Normfinder and BestKeeper tools. The overall analysis showed that UBI, followed by 18S and ACT, are the most suitable genes as internal controls for quantitative gene expression studies in MRCV-infective planthoppers, while TUB and EF1A are the most variable ones. Moreover, EF1A was upregulated by MRCV infection. Conclusions A RT-qPCR platform for gene expression analysis in the MRCV-infected planthopper vector Delphacodes kuscheli was developed. Our work is the first report on reference gene selection in virus-infected insects, and might serve as a precedent for future gene expression studies on MRCV and other virus-planthopper pathosystems.
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sequencing of the bicistronic genome segments s7 and s9 of mal de rio cuarto virus Fijivirus reoviridae completes the genome of this virus
Archives of Virology, 2007Co-Authors: F A Guzman, Ana J Distefano, Horacio Esteban Hopp, J D Arneodo, Sergio Lenardon, M Del VasAbstract:The nucleotide sequences of genomic segments S7 and S9 of Mal de Rio Cuarto virus (MRCV, Fijivirus group II) have been determined, thus completing the entire genome sequence of the virus. These segments showed a non-overlapping bicistronic structure, as in other members of the genus. MRCV S7 ORF-1 had a length of 1086 bp and encoded a 41.5 kDa putative polypeptide, whereas MRCV S7 ORF-2 had a length of 930 bp and encoded a 36.8 kDa putative polypeptide. Proteins of 39 and 20.5 kDa were predicted for the 1014 bp long MRCV S9 ORF-1 and the 537 bp long MRCV S9 ORF-2, respectively. The terminal 5′ and 3′ sequences of both segments were 5′AAGUUUUU3′ and 5′CAGCUnnnGUC3′, respectively. Specific imperfect inverted repeats of each segment were identified. Comparison of the predicted proteins with those of related virus genome segments counterparts in maize rough dwarf virus (MRDV) and rice black streaked dwarf virus (RBSDV), showed 64.5–44.3% identities. These values are lower than those resulting from comparisons between MRDV and RBSDV. The topology of the trees obtained using the complete nucleotide and amino acid sequences of MRCV S7 and MRCV S9 was consistent with the analysis of the other MRCV segments previously published.
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sequence analysis of genome segments s5 and s10 of mal de rio cuarto virus Fijivirus reoviridae
Archives of Virology, 2005Co-Authors: Ana J Distefano, Horacio Esteban Hopp, M Del VasAbstract:Mal de Rio Cuarto virus (MRCV) was recently described as a new species of the genus Fijivirus, family Reoviridae. The nucleotide sequence of two MRCV genome segments was determined. MRCV S5 and S10 were predicted to encode proteins of 106.9 and 63.5 kDa respectively. The protein coded by MRCV S5 had 62.8% and 35.7% identity to Fijiviruses RBSDV S5 and FDV S5 coded proteins, and contained a rarely reported type-1 C-terminal peroxisomal targeting signal. The protein coded by MRCV S10 had identity levels of 72.4% and 21.7% to the major outer capsid proteins of Fijiviruses RBSDV S10 and NLRV S8.
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sequence analysis of genome segments s4 and s8 of mal de rio cuarto virus mrcv evidence that the virus should be a separate Fijivirus species
Archives of Virology, 2002Co-Authors: Ana J Distefano, Horacio Esteban Hopp, L R Conci, Munoz M Hidalgo, F A Guzman, M Del VasAbstract:This is the first sequence-based characterization of Mal de Rio Cuarto virus (MRCV), currently classified as a variant of Maize rough dwarf virus (MRDV) and exclusively found in South America. We sequenced and analyzed genome segments S4 and S8. MRCV S4 coded for a putative 131.67 kDa protein while MRCV S8 coded for a putative 68.26 kDa protein containing an ATP/GTP-binding motif. The 5′ and 3′ ends of MRCV segments, were 5′AAGUUUUU3′ and 5′CAGCUnnnGUC3′, respectively. Prediction of secondary structure of both segments coding strands showed that terminal regions were able to form structures that are proposed to be replication and packaging signals. MRCV S4 showed identity to members of Fijivirus as well as to two other genera of the Reoviridae family. MRCV S8 revealed identity with Rice black streaked dwarf virus (RBSDV) S8, MRDV S7, Oat sterile dwarf virus (OSDV) S9 and Nilaparvata lugens reovirus (NLRV) S7. While MRDV and RBSDV segments are highly homologous between each other, MRCV identity levels with them was considerably lower. We discussed the evolutionary relationships of MRCV to other Reoviridae, and based on phylogenetic analysis we proposed that although MRCV is related to MRDV, it could be regarded as a new species of the Fijivirus genus.
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Sequence and phylogenetic analysis of genome segments
2002Co-Authors: Ana J Distefano, Horacio Esteban Hopp, L R Conci, Munoz M Hidalgo, F A Guzman, M Del VasAbstract:Abstract Mal de Río Cuarto virus (MRCV) is a newly described species of the genus Fijivirus , family Reoviridae . The nucleotide sequence of four MRCV genome segments was determined. MRCV S1, S2, S3 and S6 were predicted to encode proteins of 168.4, 134.4, 141.7 and 90 kDa, respectively. MRCV S1 encodes a basic protein that contains conserved RNA-dependent RNA polymerase motifs, and is homologous to Rice black streaked dwarf virus (RBSDV), Fiji disease virus (FDV) and Nilaparvata lugens reovirus (NLRV) polymerases as well as to corresponding proteins of members of other genera of the Reoviridae . MRCV S2 codes for a protein with intermediate homology to the ones coded by RBSDV S4 and FDV S3 'B' spike, which is presumably the B-spike protein. MRCV S3 most probably encodes the major core protein and is highly homologous to corresponding proteins of RBSDV S2 and FDV S3. MRCV S6-encoded protein has low homology to the proteins of unknown function coded by RBSDV S6 and FDV S6. The identity levels between all analyzed MRCV coded proteins and their RBSDV counterparts varied between 84.5 and 44.8%. The analysis of the reported sequences allowed a phylogenetic comparison of MRCV with other reovirus and supported its taxonomic status within the genus.
Ana J Distefano - One of the best experts on this subject based on the ideXlab platform.
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functional and biochemical properties of mal de rio cuarto virus Fijivirus reoviridae p9 1 viroplasm protein show further similarities to animal reovirus counterparts
Virus Research, 2010Co-Authors: Guillermo Andres Maroniche, Ana J Distefano, Vanesa C Mongelli, Andrea Peralta, Gabriela Llauger, Oscar Taboga, Esteban HoppAbstract:Mal de Rio Cuarto virus (MRCV) is a plant virus of the genus Fijivirus within the family Reoviridae that infects several monocotyledonous species and is transmitted by planthoppers in a persistent and propagative manner. Other members of the family replicate in viral inclusion bodies (VIBs) termed viroplasms that are formed in the cytoplasm of infected plant and insect cells. In this study, the protein coded by the first ORF of MRCV segment S9 (P9-1) was shown to establish cytoplasmic inclusion bodies resembling viroplasms after transfection of Spodoptera frugiperda insect cells. In accordance, MRCV P9-1 self-associates giving rise to high molecular weight complexes when expressed in bacteria. Strong self-interaction was also evidenced by yeast two-hybrid assays. Furthermore, biochemical characterization showed that MRCV P9-1 bound single stranded RNA and had ATPase activity. Finally, the MRCV P9-1 region required for the formation of VIB-like structures was mapped to the protein carboxy-terminal half. This extensive functional and biochemical characterization of MRCV P9-1 revealed further similarities between plant and animal reovirus viroplasm proteins.
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sequencing of the bicistronic genome segments s7 and s9 of mal de rio cuarto virus Fijivirus reoviridae completes the genome of this virus
Archives of Virology, 2007Co-Authors: F A Guzman, Ana J Distefano, Horacio Esteban Hopp, J D Arneodo, Sergio Lenardon, M Del VasAbstract:The nucleotide sequences of genomic segments S7 and S9 of Mal de Rio Cuarto virus (MRCV, Fijivirus group II) have been determined, thus completing the entire genome sequence of the virus. These segments showed a non-overlapping bicistronic structure, as in other members of the genus. MRCV S7 ORF-1 had a length of 1086 bp and encoded a 41.5 kDa putative polypeptide, whereas MRCV S7 ORF-2 had a length of 930 bp and encoded a 36.8 kDa putative polypeptide. Proteins of 39 and 20.5 kDa were predicted for the 1014 bp long MRCV S9 ORF-1 and the 537 bp long MRCV S9 ORF-2, respectively. The terminal 5′ and 3′ sequences of both segments were 5′AAGUUUUU3′ and 5′CAGCUnnnGUC3′, respectively. Specific imperfect inverted repeats of each segment were identified. Comparison of the predicted proteins with those of related virus genome segments counterparts in maize rough dwarf virus (MRDV) and rice black streaked dwarf virus (RBSDV), showed 64.5–44.3% identities. These values are lower than those resulting from comparisons between MRDV and RBSDV. The topology of the trees obtained using the complete nucleotide and amino acid sequences of MRCV S7 and MRCV S9 was consistent with the analysis of the other MRCV segments previously published.
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sequence analysis of genome segments s5 and s10 of mal de rio cuarto virus Fijivirus reoviridae
Archives of Virology, 2005Co-Authors: Ana J Distefano, Horacio Esteban Hopp, M Del VasAbstract:Mal de Rio Cuarto virus (MRCV) was recently described as a new species of the genus Fijivirus, family Reoviridae. The nucleotide sequence of two MRCV genome segments was determined. MRCV S5 and S10 were predicted to encode proteins of 106.9 and 63.5 kDa respectively. The protein coded by MRCV S5 had 62.8% and 35.7% identity to Fijiviruses RBSDV S5 and FDV S5 coded proteins, and contained a rarely reported type-1 C-terminal peroxisomal targeting signal. The protein coded by MRCV S10 had identity levels of 72.4% and 21.7% to the major outer capsid proteins of Fijiviruses RBSDV S10 and NLRV S8.
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sequence analysis of genome segments s4 and s8 of mal de rio cuarto virus mrcv evidence that the virus should be a separate Fijivirus species
Archives of Virology, 2002Co-Authors: Ana J Distefano, Horacio Esteban Hopp, L R Conci, Munoz M Hidalgo, F A Guzman, M Del VasAbstract:This is the first sequence-based characterization of Mal de Rio Cuarto virus (MRCV), currently classified as a variant of Maize rough dwarf virus (MRDV) and exclusively found in South America. We sequenced and analyzed genome segments S4 and S8. MRCV S4 coded for a putative 131.67 kDa protein while MRCV S8 coded for a putative 68.26 kDa protein containing an ATP/GTP-binding motif. The 5′ and 3′ ends of MRCV segments, were 5′AAGUUUUU3′ and 5′CAGCUnnnGUC3′, respectively. Prediction of secondary structure of both segments coding strands showed that terminal regions were able to form structures that are proposed to be replication and packaging signals. MRCV S4 showed identity to members of Fijivirus as well as to two other genera of the Reoviridae family. MRCV S8 revealed identity with Rice black streaked dwarf virus (RBSDV) S8, MRDV S7, Oat sterile dwarf virus (OSDV) S9 and Nilaparvata lugens reovirus (NLRV) S7. While MRDV and RBSDV segments are highly homologous between each other, MRCV identity levels with them was considerably lower. We discussed the evolutionary relationships of MRCV to other Reoviridae, and based on phylogenetic analysis we proposed that although MRCV is related to MRDV, it could be regarded as a new species of the Fijivirus genus.
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Sequence and phylogenetic analysis of genome segments
2002Co-Authors: Ana J Distefano, Horacio Esteban Hopp, L R Conci, Munoz M Hidalgo, F A Guzman, M Del VasAbstract:Abstract Mal de Río Cuarto virus (MRCV) is a newly described species of the genus Fijivirus , family Reoviridae . The nucleotide sequence of four MRCV genome segments was determined. MRCV S1, S2, S3 and S6 were predicted to encode proteins of 168.4, 134.4, 141.7 and 90 kDa, respectively. MRCV S1 encodes a basic protein that contains conserved RNA-dependent RNA polymerase motifs, and is homologous to Rice black streaked dwarf virus (RBSDV), Fiji disease virus (FDV) and Nilaparvata lugens reovirus (NLRV) polymerases as well as to corresponding proteins of members of other genera of the Reoviridae . MRCV S2 codes for a protein with intermediate homology to the ones coded by RBSDV S4 and FDV S3 'B' spike, which is presumably the B-spike protein. MRCV S3 most probably encodes the major core protein and is highly homologous to corresponding proteins of RBSDV S2 and FDV S3. MRCV S6-encoded protein has low homology to the proteins of unknown function coded by RBSDV S6 and FDV S6. The identity levels between all analyzed MRCV coded proteins and their RBSDV counterparts varied between 84.5 and 44.8%. The analysis of the reported sequences allowed a phylogenetic comparison of MRCV with other reovirus and supported its taxonomic status within the genus.
Jian Yang - One of the best experts on this subject based on the ideXlab platform.
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interaction between southern rice black streaked dwarf virus minor core protein p8 and a rice zinc finger transcription factor
Archives of Virology, 2017Co-Authors: Nianjun Cai, Jian Yang, Jianping Chen, Jin Xue, Heng-mu ZhangAbstract:The Fijivirus southern rice black-streaked dwarf virus (SRBSDV) causes one of the most serious viral diseases of rice in China and Vietnam. To better understand the molecular basis of SRBSDV infection, a yeast two-hybrid screen of a rice cDNA library was carried out using P8, a minor core protein of SRBSDV, as the bait. A rice Cys2His2-type zinc finger protein (OsZFP) was found to interact with SRBSDV P8. A strong interaction between SRBSDV P8 and OsZFP was then confirmed by pull-down assays, and bimolecular fluorescence complementation assays showed that the in vivo interaction was specifically localized in the nucleus of plant cells. Using a series of deletion mutants, it was shown that both the NTP-binding region of P8 and the first two zinc fingers of OsZFP were crucial for their interaction in plant cells. The localization in the nucleus and activation of transcription in yeast supports the notion that OsZFP is a transcription factor. SRBSDV P8 may play an important role in fijiviral infection and symptom development by interfering with the host transcription activity of OsZFP.
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complete genomic sequence of maize rough dwarf virus a Fijivirus transmitted by the small brown planthopper
Genome Announcements, 2016Co-Authors: Li Xie, Jian Yang, Jianping Chen, Heng-mu ZhangAbstract:The nucleotide sequences of the 10 genomic segments of an Italian isolate of maize rough dwarf virus (MRDV) were determined. This first complete genomic sequence of MRDV will help understand the phylogenetic relationships among group 2 Fijiviruses and especially the closely related rice black-streaked dwarf virus, which is also found to naturally infect maize.
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p5 2 of rice black streaked dwarf virus is a non structural protein targeted to chloroplasts
Archives of Virology, 2015Co-Authors: Xiaoya Liu, Jianping Chen, Jian Yang, Li Xie, Xi-jiao Song, Heng-mu ZhangAbstract:The genome segment S5 of rice black-streaked dwarf virus (genus Fijivirus, family Reoviridae) is functionally bicistronic in infected plants. It has a conserved second ORF (P5-2) partially overlapping the major ORF in a different reading frame, but its function remains unknown. P5-2 was detected in infected plants, but not in purified viral particles by Western blotting, indicating that it is a non-structural protein. In immunoelectron microscopy, polyclonal antibodies against P5-2 specifically labelled chloroplasts of infected rice plants. When P5-2 fused with green fluorescent protein was transiently expressed in leaves of Nicotiana benthamiana, fluorescence was also co-localized with chloroplasts. Experiments with deletion mutants of P5-2 showed that its N-terminal part was responsible for its targeting to chloroplasts.
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characterization of homologous and heterologous interactions between viroplasm proteins p6 and p9 1 of the Fijivirus southern rice black streaked dwarf virus
Archives of Virology, 2015Co-Authors: Jin Xue, Heng-mu Zhang, Jian Yang, Li Xie, Jianping ChenAbstract:P6 of southern rice black-streaked dwarf virus (SRBSDV) is a multifunctional protein that is involved in the formation of viroplasms by interacting with P5-1. Here, we used yeast two-hybrid and bimolecular fluorescence complementation assays to show that there were homologous and heterologous interactions between SRBSDV P6 and P9-1 in yeast and plant cells. Mutational analysis showed that the N-terminal region (residues 1–93) of P6 was necessary for the interaction between P6 and P9-1. Self-interactions only occurred between the full-length P6 or P9-1. P9-1 was able to form viroplasm-like inclusion structures alone in the absence of other viral proteins.
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molecular characterization of southern rice blacked streaked dwarf virus srbsdv from vietnam
Journal of Phytopathology, 2014Co-Authors: Jin Xue, Heng-mu Zhang, Jian Yang, Bida Gao, Jianping ChenAbstract:AbstractSouthern rice black-streaked dwarf virus (SRBSDV) is a novel putativemember of the genus Fijivirus, family Reoviridae. We report here the geno-mic sequences of a Vietnamese isolate (SRBSDV-V). The total genome ofSRBSDV-V has 29 115 nucleotides (nt), nine nt shorter than SRBSDV-GDor -HN, but similar in organization to these two Chinese isolates. Nucleo-tide diversities among SRBSDV isolates were much lower than thoseamong the corresponding ORFs of the available RBSDV isolates and therewas a lower purifying selection pressure on SRBSDV than RBSDV, provid-ing first molecular evidence for the view that SRBSDV is of recent origin.In studies of all available SRBSDV sequences, there was no obvious corre-lation between geographic distances and phylogenetic distribution. A highfrequency of genetic recombination was found among both Chinese andVietnam SRBSDV isolates, suggesting that recombination may play animportant role in the molecular variation and evolution of SRBSDV.IntroductionSouthern rice black-streaked dwarf virus (SRBSDV)(or rice black-streaked dwarf virus 2), was firstreported in the Chinese provinces of Hainan andGuangdong in 2001 and was initially considered anisolate of RBSDV, because it has similar particle mor-phology and serology and induces similar symptomson host plants of those of RBSDV (Zhou et al. 2004).However, based on the sequence analysis and differ-ences in vector specificity, the virus is now considereddistinct and was identified as a novel putative memberof the genus Fijivirus (Zhang et al. 2008b; Zhou et al.2008), which is one of three plant-infecting genera inthe large family Reoviridae (Boccardo and Milne 1984;Attoui et al. 2012). In contrast to RBSDV, SRBSDV isefficiently transmitted by the white-backed planthop-per (WBPH; Sogatella furcimera Horvath), which is awidespread migratory pest throughout the mainrice-growing areas in China (Shen et al. 2003), in apropagative persistent manner (Zhou et al. 2008;Li et al. 2012), and only poorly transmitted by thesmall planthopper (Laodelphax striatellus Fallen), themain vector of RBSDV (Boccardo and Milne 1984;Attoui et al. 2012). Since 2009, the disease causedby SRBSDV has become one of the most important