The Experts below are selected from a list of 4026 Experts worldwide ranked by ideXlab platform
Sezgin Bakırdere - One of the best experts on this subject based on the ideXlab platform.
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quadruple isotope dilution gas chromatography mass spectrometry after simultaneous derivatization and spraying based Fine Droplet formation liquid phase microextraction method for the accurate and sensitive quantification of chloroquine phosphate in human serum urine and saliva samples at trace levels
Journal of Chromatography A, 2021Co-Authors: Sezin Erarpat, Süleyman Bodur, Omer Tahir Gunkara, Sezgin Bakırdere, Miray OnerAbstract:Abstract This study presents an accurate and precise analytical strategy for the determination of chloroquine phosphate at trace levels in human body fluids (urine, serum, and saliva). Simultaneous derivatization-spraying based Fine Droplet formation-liquid phase microextraction (SD-SFDF-LPME) method was used to derivatize and preconcentrate the analyte prior to gas chromatography-mass spectrometry (GC–MS) measurements. Acetic anhydride was employed as derivatizing agent in this study. After optimizing the SD-SFDF-LPME method, the limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.16 and 0.53 mg/kg, respectively. Quadruple isotope dilution (ID4) was coupled to the SD-SFDF-LPME method in order to alleviate matrix effects and promote accuracy/precision of the method. Chloroquine acetamide-d3 was firstly synthesized in our research laboratory and used as the isotopic analogue of the analyte in the ID4 experiments. Superior percent recovery results (99.4% – 101.0%) with low standard deviation values were obtained for the spiked samples. This validated the developed SD-SFDF-LPME-ID4-GC–MS method as highly accurate and precise for the determination of chloroquine phosphate at trace levels. In addition, the isotopic analogue of the analyte was obtained via the acetamide derivative of the analyte, which is an alternative to obtain isotopic analogues of organic compounds that are not accessible or commercially available.
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Accurate and sensitive determination of hydroxychloroquine sulfate used on COVID-19 patients in human urine, serum and saliva samples by GC-MS.
Journal of pharmaceutical analysis, 2021Co-Authors: Süleyman Bodur, Sezin Erarpat, Omer Tahir Gunkara, Sezgin BakırdereAbstract:Abstract A rapid, accurate, and sensitive analytical method, ultrasonication-assisted spraying based Fine Droplet formation–liquid phase microextraction–gas chromatography–mass spectrometry (UA-SFDF-LPME-GC-MS), was proposed for the determination of trace amounts of hydroxychloroquine sulfate in human serum, urine, and saliva samples. To determine the best extraction strategy, several liquid and solid phase extraction methods were investigated for their efficiencies in isolation and preconcentration of hydroxychloroquine sulfate from biological matrices. The UA-SFDF-LPME method was determined to be the best extraction method as it was operationally simple and provided accurate results. Variables such as the extraction solvent, spraying number, sodium hydroxide concentration and volume, sample volume, mixing method, and mixing period were optimized for the proposed method using the one-variable-at-a-time approach. In addition, Tukey’s method based on a post hoc comparison test was employed to evaluate the significant difference between the parameters inspected. After the optimization studies, the limit of detection (LOD) and limit of quantification (LOQ) were determined to be 0.7 and 2.4 μg/kg, respectively. The sensitivity of the GC-MS system based on the LOD was enhanced approximately 440-fold when the UA-SFDF-LPME method was employed. Spiking experiments were also conducted for the human serum, urine, and saliva samples to determine the applicability and accuracy of the proposed method. Recoveries for the human serum, urine, and saliva samples were found to be in the ranges 94%–102%, 95%–105%, and 93%–102%, respectively. These results were satisfactory and indicated that the hydroxychloroquine sulfate level in the above biological samples could be analyzed using the proposed method.
Sezin Erarpat - One of the best experts on this subject based on the ideXlab platform.
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quadruple isotope dilution gas chromatography mass spectrometry after simultaneous derivatization and spraying based Fine Droplet formation liquid phase microextraction method for the accurate and sensitive quantification of chloroquine phosphate in human serum urine and saliva samples at trace levels
Journal of Chromatography A, 2021Co-Authors: Sezin Erarpat, Süleyman Bodur, Omer Tahir Gunkara, Sezgin Bakırdere, Miray OnerAbstract:Abstract This study presents an accurate and precise analytical strategy for the determination of chloroquine phosphate at trace levels in human body fluids (urine, serum, and saliva). Simultaneous derivatization-spraying based Fine Droplet formation-liquid phase microextraction (SD-SFDF-LPME) method was used to derivatize and preconcentrate the analyte prior to gas chromatography-mass spectrometry (GC–MS) measurements. Acetic anhydride was employed as derivatizing agent in this study. After optimizing the SD-SFDF-LPME method, the limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.16 and 0.53 mg/kg, respectively. Quadruple isotope dilution (ID4) was coupled to the SD-SFDF-LPME method in order to alleviate matrix effects and promote accuracy/precision of the method. Chloroquine acetamide-d3 was firstly synthesized in our research laboratory and used as the isotopic analogue of the analyte in the ID4 experiments. Superior percent recovery results (99.4% – 101.0%) with low standard deviation values were obtained for the spiked samples. This validated the developed SD-SFDF-LPME-ID4-GC–MS method as highly accurate and precise for the determination of chloroquine phosphate at trace levels. In addition, the isotopic analogue of the analyte was obtained via the acetamide derivative of the analyte, which is an alternative to obtain isotopic analogues of organic compounds that are not accessible or commercially available.
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Accurate and sensitive determination of hydroxychloroquine sulfate used on COVID-19 patients in human urine, serum and saliva samples by GC-MS.
Journal of pharmaceutical analysis, 2021Co-Authors: Süleyman Bodur, Sezin Erarpat, Omer Tahir Gunkara, Sezgin BakırdereAbstract:Abstract A rapid, accurate, and sensitive analytical method, ultrasonication-assisted spraying based Fine Droplet formation–liquid phase microextraction–gas chromatography–mass spectrometry (UA-SFDF-LPME-GC-MS), was proposed for the determination of trace amounts of hydroxychloroquine sulfate in human serum, urine, and saliva samples. To determine the best extraction strategy, several liquid and solid phase extraction methods were investigated for their efficiencies in isolation and preconcentration of hydroxychloroquine sulfate from biological matrices. The UA-SFDF-LPME method was determined to be the best extraction method as it was operationally simple and provided accurate results. Variables such as the extraction solvent, spraying number, sodium hydroxide concentration and volume, sample volume, mixing method, and mixing period were optimized for the proposed method using the one-variable-at-a-time approach. In addition, Tukey’s method based on a post hoc comparison test was employed to evaluate the significant difference between the parameters inspected. After the optimization studies, the limit of detection (LOD) and limit of quantification (LOQ) were determined to be 0.7 and 2.4 μg/kg, respectively. The sensitivity of the GC-MS system based on the LOD was enhanced approximately 440-fold when the UA-SFDF-LPME method was employed. Spiking experiments were also conducted for the human serum, urine, and saliva samples to determine the applicability and accuracy of the proposed method. Recoveries for the human serum, urine, and saliva samples were found to be in the ranges 94%–102%, 95%–105%, and 93%–102%, respectively. These results were satisfactory and indicated that the hydroxychloroquine sulfate level in the above biological samples could be analyzed using the proposed method.
Süleyman Bodur - One of the best experts on this subject based on the ideXlab platform.
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quadruple isotope dilution gas chromatography mass spectrometry after simultaneous derivatization and spraying based Fine Droplet formation liquid phase microextraction method for the accurate and sensitive quantification of chloroquine phosphate in human serum urine and saliva samples at trace levels
Journal of Chromatography A, 2021Co-Authors: Sezin Erarpat, Süleyman Bodur, Omer Tahir Gunkara, Sezgin Bakırdere, Miray OnerAbstract:Abstract This study presents an accurate and precise analytical strategy for the determination of chloroquine phosphate at trace levels in human body fluids (urine, serum, and saliva). Simultaneous derivatization-spraying based Fine Droplet formation-liquid phase microextraction (SD-SFDF-LPME) method was used to derivatize and preconcentrate the analyte prior to gas chromatography-mass spectrometry (GC–MS) measurements. Acetic anhydride was employed as derivatizing agent in this study. After optimizing the SD-SFDF-LPME method, the limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.16 and 0.53 mg/kg, respectively. Quadruple isotope dilution (ID4) was coupled to the SD-SFDF-LPME method in order to alleviate matrix effects and promote accuracy/precision of the method. Chloroquine acetamide-d3 was firstly synthesized in our research laboratory and used as the isotopic analogue of the analyte in the ID4 experiments. Superior percent recovery results (99.4% – 101.0%) with low standard deviation values were obtained for the spiked samples. This validated the developed SD-SFDF-LPME-ID4-GC–MS method as highly accurate and precise for the determination of chloroquine phosphate at trace levels. In addition, the isotopic analogue of the analyte was obtained via the acetamide derivative of the analyte, which is an alternative to obtain isotopic analogues of organic compounds that are not accessible or commercially available.
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Accurate and sensitive determination of hydroxychloroquine sulfate used on COVID-19 patients in human urine, serum and saliva samples by GC-MS.
Journal of pharmaceutical analysis, 2021Co-Authors: Süleyman Bodur, Sezin Erarpat, Omer Tahir Gunkara, Sezgin BakırdereAbstract:Abstract A rapid, accurate, and sensitive analytical method, ultrasonication-assisted spraying based Fine Droplet formation–liquid phase microextraction–gas chromatography–mass spectrometry (UA-SFDF-LPME-GC-MS), was proposed for the determination of trace amounts of hydroxychloroquine sulfate in human serum, urine, and saliva samples. To determine the best extraction strategy, several liquid and solid phase extraction methods were investigated for their efficiencies in isolation and preconcentration of hydroxychloroquine sulfate from biological matrices. The UA-SFDF-LPME method was determined to be the best extraction method as it was operationally simple and provided accurate results. Variables such as the extraction solvent, spraying number, sodium hydroxide concentration and volume, sample volume, mixing method, and mixing period were optimized for the proposed method using the one-variable-at-a-time approach. In addition, Tukey’s method based on a post hoc comparison test was employed to evaluate the significant difference between the parameters inspected. After the optimization studies, the limit of detection (LOD) and limit of quantification (LOQ) were determined to be 0.7 and 2.4 μg/kg, respectively. The sensitivity of the GC-MS system based on the LOD was enhanced approximately 440-fold when the UA-SFDF-LPME method was employed. Spiking experiments were also conducted for the human serum, urine, and saliva samples to determine the applicability and accuracy of the proposed method. Recoveries for the human serum, urine, and saliva samples were found to be in the ranges 94%–102%, 95%–105%, and 93%–102%, respectively. These results were satisfactory and indicated that the hydroxychloroquine sulfate level in the above biological samples could be analyzed using the proposed method.
Omer Tahir Gunkara - One of the best experts on this subject based on the ideXlab platform.
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quadruple isotope dilution gas chromatography mass spectrometry after simultaneous derivatization and spraying based Fine Droplet formation liquid phase microextraction method for the accurate and sensitive quantification of chloroquine phosphate in human serum urine and saliva samples at trace levels
Journal of Chromatography A, 2021Co-Authors: Sezin Erarpat, Süleyman Bodur, Omer Tahir Gunkara, Sezgin Bakırdere, Miray OnerAbstract:Abstract This study presents an accurate and precise analytical strategy for the determination of chloroquine phosphate at trace levels in human body fluids (urine, serum, and saliva). Simultaneous derivatization-spraying based Fine Droplet formation-liquid phase microextraction (SD-SFDF-LPME) method was used to derivatize and preconcentrate the analyte prior to gas chromatography-mass spectrometry (GC–MS) measurements. Acetic anhydride was employed as derivatizing agent in this study. After optimizing the SD-SFDF-LPME method, the limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.16 and 0.53 mg/kg, respectively. Quadruple isotope dilution (ID4) was coupled to the SD-SFDF-LPME method in order to alleviate matrix effects and promote accuracy/precision of the method. Chloroquine acetamide-d3 was firstly synthesized in our research laboratory and used as the isotopic analogue of the analyte in the ID4 experiments. Superior percent recovery results (99.4% – 101.0%) with low standard deviation values were obtained for the spiked samples. This validated the developed SD-SFDF-LPME-ID4-GC–MS method as highly accurate and precise for the determination of chloroquine phosphate at trace levels. In addition, the isotopic analogue of the analyte was obtained via the acetamide derivative of the analyte, which is an alternative to obtain isotopic analogues of organic compounds that are not accessible or commercially available.
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Accurate and sensitive determination of hydroxychloroquine sulfate used on COVID-19 patients in human urine, serum and saliva samples by GC-MS.
Journal of pharmaceutical analysis, 2021Co-Authors: Süleyman Bodur, Sezin Erarpat, Omer Tahir Gunkara, Sezgin BakırdereAbstract:Abstract A rapid, accurate, and sensitive analytical method, ultrasonication-assisted spraying based Fine Droplet formation–liquid phase microextraction–gas chromatography–mass spectrometry (UA-SFDF-LPME-GC-MS), was proposed for the determination of trace amounts of hydroxychloroquine sulfate in human serum, urine, and saliva samples. To determine the best extraction strategy, several liquid and solid phase extraction methods were investigated for their efficiencies in isolation and preconcentration of hydroxychloroquine sulfate from biological matrices. The UA-SFDF-LPME method was determined to be the best extraction method as it was operationally simple and provided accurate results. Variables such as the extraction solvent, spraying number, sodium hydroxide concentration and volume, sample volume, mixing method, and mixing period were optimized for the proposed method using the one-variable-at-a-time approach. In addition, Tukey’s method based on a post hoc comparison test was employed to evaluate the significant difference between the parameters inspected. After the optimization studies, the limit of detection (LOD) and limit of quantification (LOQ) were determined to be 0.7 and 2.4 μg/kg, respectively. The sensitivity of the GC-MS system based on the LOD was enhanced approximately 440-fold when the UA-SFDF-LPME method was employed. Spiking experiments were also conducted for the human serum, urine, and saliva samples to determine the applicability and accuracy of the proposed method. Recoveries for the human serum, urine, and saliva samples were found to be in the ranges 94%–102%, 95%–105%, and 93%–102%, respectively. These results were satisfactory and indicated that the hydroxychloroquine sulfate level in the above biological samples could be analyzed using the proposed method.
Ryan S Henry - One of the best experts on this subject based on the ideXlab platform.
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measuring Droplet size of agricultural spray nozzles measurement distance and airspeed effects
Atomization and Sprays, 2014Co-Authors: Bradley K. Fritz, W E Bagley, Clint W Hoffmann, Zbigniew Czaczyk, Greg R. Kruger, Ryan S HenryAbstract:With a number of new spray testing laboratories going into operation and each gearing up to measure spray atomization from agricultural spray nozzles using laser diffraction, establishing and following a set of scientific standard procedures is crucial to long-term data generation and standardization across the industry. It has long been recognized that while offering ease of use as compared to other methods, laser diffraction measurements do not account for measurement bias effects due to differential velocities between differing sized spray Droplets, and in many cases significantly overestimate the Fine Droplet portion of the spray. Droplet sizes and velocities were measured for three agricultural flat fan nozzles (8002, 8008, and 6510) each at three spray pressures (138, 276, and 414 kPa) at four downstream distances (15.2, 30.5, 45.7, and 76.2 cm) across a range of concurrent air velocities (0.7–80.5 m/s). At air velocities below 6.7 m/s, large gradients in Droplet velocities resulted in overestimation of both the 10% volume diameter (Dv0.1) by more than 10% and the percent volume of the spray less than 100 μm (V<100) was overestimated twoto three-fold. The optimal measurement distance to reduce Droplet measurement bias to less than 5% was found to be 30.5 cm with a concurrent air velocity of 6.7 m/s for measuring Droplet size from ground nozzles. For aerial spray nozzles, the optimal distance was 45.7 cm. Use of these methods provides for more accurate Droplet size data for use in efficacy testing and drift assessments, and significantly increases inter-lab reproducibility.