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Mojtaba Raeisi - One of the best experts on this subject based on the ideXlab platform.
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using wpc inulin fucoidan complexes for encapsulation of Fish Protein Hydrolysate and Fish oil in w1 o w2 emulsion characterization and nutritional quality
Food Research International, 2018Co-Authors: Aniseh Jamshidi, Bahareh Shabanpour, Parastoo Pourashouri, Mojtaba RaeisiAbstract:Abstract The double emulsions and freeze-dried microcapsules containing Fish Protein Hydrolysate (FPH) and Fish oil (FO) were stabilized by complexs of whey Protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release. Higher encapsulation efficiency and solubility were observed in Fuc-WPC microcapsules (86.31% and 30.26 mg/100 g, respectively). The combination of Fuc-WPC in the wall material showed higher oxidative stability than other wall material. The higher values of PUFA and SFA were observed in Inu-WPC Fuc-WPC microcapsules, respectively. The Fuc-WPC and Inu + Fuc-WPC micrographs showed a more porous structure compared to Inu-WPC. The mean particle size ranged from 536.8 ± 52.70 to 842.36 ± 21.41 nm. No significant differences were observed in the released oil and the fatty acid composition during gastrointestinal digestion. Sensory evaluation of fortified natural yogurt with microcapsules showed lower Fishy flavor in Inu-WPC samples than those fortified with Fuc-WPC and Inu + Fuc-WPC. In general, the use of inulin with WPC as a wall materal resulted in good characteristics and sensory attributed, although the use of fucoidan with WPC conferred higher oxidative stability during storage.
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Using WPC-inulin-fucoidan complexes for encapsulation of Fish Protein Hydrolysate and Fish oil in W1/O/W2 emulsion: Characterization and nutritional quality.
Food Research International, 2018Co-Authors: Aniseh Jamshidi, Bahareh Shabanpour, Parastoo Pourashouri, Mojtaba RaeisiAbstract:Abstract The double emulsions and freeze-dried microcapsules containing Fish Protein Hydrolysate (FPH) and Fish oil (FO) were stabilized by complexs of whey Protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release. Higher encapsulation efficiency and solubility were observed in Fuc-WPC microcapsules (86.31% and 30.26 mg/100 g, respectively). The combination of Fuc-WPC in the wall material showed higher oxidative stability than other wall material. The higher values of PUFA and SFA were observed in Inu-WPC Fuc-WPC microcapsules, respectively. The Fuc-WPC and Inu + Fuc-WPC micrographs showed a more porous structure compared to Inu-WPC. The mean particle size ranged from 536.8 ± 52.70 to 842.36 ± 21.41 nm. No significant differences were observed in the released oil and the fatty acid composition during gastrointestinal digestion. Sensory evaluation of fortified natural yogurt with microcapsules showed lower Fishy flavor in Inu-WPC samples than those fortified with Fuc-WPC and Inu + Fuc-WPC. In general, the use of inulin with WPC as a wall materal resulted in good characteristics and sensory attributed, although the use of fucoidan with WPC conferred higher oxidative stability during storage.
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Evaluation of Different Proportions of Distilled Water to Substrate on Functional Properties, Antioxidant and Nutritional Quality of Bigeye Ilisha (Ilisha Megaloptera) Protein Hydrolysate
Golestan University of Medical Sciences, 2018Co-Authors: Aniseh Jamshidi, Bahareh Shabanpour, Parastoo Pourashouri, Mojtaba RaeisiAbstract:Background and Objectives: Production of Fish Protein Hydrolysate is a method for converting the low-value economical underutilized Fish species to value-added products. This study aimed to evaluate the different proportions of distilled water to the substrate on the functional characteristics, antioxidant and nutritional quality of Fish Protein Hydrolysate of bigeye ilisha (Ilisha megaloptera) produced by enzymatic hydrolysis. Materials and Methods: After defatting of minced Fish, the hydrolysis process was carried out using three different 4:1, 5:1 and 6:1 distilled water to substrate proportions by using alcalase enzyme in three replications. The Protein Hydrolysate samples were analyzed for approximate composition (soluble Protein, moisture, fat and ash), functional characteristics (solubility, foam capacity, and foam stability), antioxidant properties (DPPH radical scavenging activity and reducing power) and mineral composition. Results: In this study, the ratio of distilled water to the substrate affected the Protein Hydrolysate properties and the highest amounts of hydrolysis degree and DPPH radical scavenging activity were observed in samples with 5:1 ratio. Fish Protein Hydrolysate obtained from 4:1 ratio had the highest amount of soluble Protein, and no significant difference was observed in term of solubility with samples obtained by 5:1 ratio. Moreover, there were no significant differences in terms of lightness and foam capacity of samples obtained from 4:1 and 6:1 proportions. Conclusion: According to the results of the study, using a 4:1 ratio of distilled water to substrate led to the production of Fish Protein Hydrolysates from bigeye ilisha with higher functional properties and nutritional composition
Keke Zheng - One of the best experts on this subject based on the ideXlab platform.
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1h nmr based metabolomics studies on the effect of size fractionated Fish Protein Hydrolysate Fish meal and plant Protein in diet for juvenile turbot scophthalmus maximus l
Aquaculture Nutrition, 2017Co-Authors: Yuliang Wei, Mengqing Liang, Kangsen Mai, Keke ZhengAbstract:This study was designed to evaluate changes in the metabolic profile of liver and muscle of turbot (Scophthalmus maximus L.) fed Fishmeal-based diet, diets containing size-fractionated Fish Protein Hydrolysate and plant Protein-based diet using 1H NMR-based metabolomics approach combined with the growth. Fish Protein Hydrolysate (FPH) was obtained by enzymatic treatment, permeate fraction was obtained as UF by ultrafiltered step, and retentate fraction was retained as RF. FM diet contained Fish meal used as a single Protein source. Four other diets (PP, UF, FPH and RF) contained 180 g kg−1 diet Fish meal. 54, 55 and 55 g kg−1 dry diet UF, FPH and RF were supplemented to UF, FPH and RF diets. All diets were formulated to be isolipidic and isonitrogenous fed to five triplicate groups of turbot (16.05 ± 0.03 g) for 68 days. O-PLS-DA in FM versus UF, FM versus FPH, FM versus RF and FM versus PP resulted in a reliable model for muscle and liver tissue, while O-PLS-DA in UF versus FPH and UF versus RF only showed metabolites changes in liver tissue. Results indicated that metabolite changes among the different treatments were consistent with the growth tendency.
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The effect of ultrafiltered Fish Protein Hydrolysate levels on the liver and muscle metabolic profile of juvenile turbot (Scophthalmus maximus L.) by 1H NMR‐based metabolomics studies
Aquaculture Research, 2016Co-Authors: Yuliang Wei, Mengqing Liang, Kangsen Mai, Keke ZhengAbstract:A 1H NMR-based metabolomics approach was to explore the effect of ultrafiltered Fish Protein Hydrolysate (UF) levels on the liver and muscle metabolic profile of juvenile turbot (Scophthalmus maximus L.). Fish Protein Hydrolysate (FPH) was produced from by-products by enzymatic treatment, and UF was obtained by diluting FPH followed filtration. Fish were fed diets containing Fish meal Protein, which was, respectively, replaced by UF Protein 0, 50, 100, 150 and 200 g kg−1 of dietary total Protein (UF-0, UF-5, UF-10, UF-15 and UF-20) for 68 days. OPLS-DA of liver and muscle showed that high levels of UF in diets may lead to corresponding tissue metabolites changes. In liver tissue, changing metabolites included dimethylamine, N,N-dimethylglycine, valine, isoleucine, leucine, arginine and alanine and were mainly involved in choline metabolism and amino acid metabolism. For muscle tissue, changing metabolites included lactate, alanine, proline, fumarate, tyrosine, histidine, cystathionine and taurine. And the metabolites were mainly involved in glycolysis and gluconeogenesis, proline metabolism and taurine metabolism. 1H NMR-based metabolomics is a useful approach to investigate different levels of UF on metabolic profile in liver and muscle tissues combined with the growth.
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1H NMR‐based metabolomics studies on the effect of size‐fractionated Fish Protein Hydrolysate, Fish meal and plant Protein in diet for juvenile turbot (Scophthalmus maximus L.)
Aquaculture Nutrition, 2016Co-Authors: Yuliang Wei, Mengqing Liang, Kangsen Mai, Keke ZhengAbstract:This study was designed to evaluate changes in the metabolic profile of liver and muscle of turbot (Scophthalmus maximus L.) fed Fishmeal-based diet, diets containing size-fractionated Fish Protein Hydrolysate and plant Protein-based diet using 1H NMR-based metabolomics approach combined with the growth. Fish Protein Hydrolysate (FPH) was obtained by enzymatic treatment, permeate fraction was obtained as UF by ultrafiltered step, and retentate fraction was retained as RF. FM diet contained Fish meal used as a single Protein source. Four other diets (PP, UF, FPH and RF) contained 180 g kg−1 diet Fish meal. 54, 55 and 55 g kg−1 dry diet UF, FPH and RF were supplemented to UF, FPH and RF diets. All diets were formulated to be isolipidic and isonitrogenous fed to five triplicate groups of turbot (16.05 ± 0.03 g) for 68 days. O-PLS-DA in FM versus UF, FM versus FPH, FM versus RF and FM versus PP resulted in a reliable model for muscle and liver tissue, while O-PLS-DA in UF versus FPH and UF versus RF only showed metabolites changes in liver tissue. Results indicated that metabolite changes among the different treatments were consistent with the growth tendency.
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graded levels of Fish Protein Hydrolysate in high plant diets for turbot scophthalmus maximus effects on growth performance and lipid accumulation
Aquaculture, 2016Co-Authors: Houguo Xu, Mengqing Liang, Yuchao Mu, Yue Zhang, Jianqiang Li, Keke ZhengAbstract:Abstract A 12-week feeding experiment in indoor flowing seawater system was conducted to investigate the effects of graded levels of dietary Fish Protein Hydrolysate (FPH) on growth performance and lipid accumulation of juvenile turbot ( Scophthalmus maximus ) (initial body weight 4.16 ± 0.01 g). Four isonitrogenous and isoenergetic experimental diets with high plant Protein were formulated to contain graded levels of FPH, meanwhile the Fish meal was replaced correspondingly by 0% (Diet FPH-0, control), 5% (Diet FPH-5), 10% (Diet FPH-10) and 20% (Diet FPH-20) of total dietary Protein, respectively. Quadruplicate groups of 25 Fish were fed to apparent satiation twice daily during the feeding trial. The results showed that the specific growth rate (SGR), Protein efficiency ratio, and Protein retention was not significantly different among group FPH-0, FPH-5, and FPH-10. The highest level of dietary FPH (FPH-20) significantly reduced the SGR but increased the feed intake compared to the control group. The viscerosomatic index in group FPH-10 and FPH-20 were significantly lower than that in the control group. Fish fed FPH-20 also showed significantly lower crude lipid concentration in whole body than Fish fed the control diet. The concentrations of total and neutral lipid in gut were significantly lower in Fish fed FPH-10 compared to the control group. For muscle lipid, the polar lipid concentration significantly decreased while the neutral lipid concentration significantly increased with increasing levels of dietary FPH, but no significant difference in total lipid concentration was observed among experimental groups. The lipid concentrations in liver were not significantly different among dietary treatments. With increasing levels of dietary FPH, serum triacylglycerol and cholesterol concentrations significantly decreased. The influence of dietary FPH on tissue fatty acid compositions generally corresponded with that on tissue lipid concentrations. In conclusion, these results suggested that in high plant Protein diets FPH replacing Fish meal by 10% of total dietary Protein did not compromise the growth of juvenile turbot. However, a higher FPH level (replacing Fish meal by 20% of total dietary Protein) reduced the growth and feed utilization but it increased the feed intake. In the present experimental conditions, the FPH treatments, especially at high levels, significantly modulated the lipid accumulation and fatty acid compositions in turbot tissues, in a dose- and tissue-dependent manner. Statement of Relevance This study provided useful data for the circulatory use of Fish processing by-products in Fish diets. Also, based on the results of lipid analysis in Fish tissues, potential lipid manipulating feed additives could be explored from Fish Protein Hydrolysates.
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The effect of ultrafiltered Fish Protein Hydrolysate level on growth performance, Protein digestibility and mRNA expression of PepT1 in juvenile turbot (Scophthalmus maximus L.)
Aquaculture Nutrition, 2015Co-Authors: Yuliang Wei, Mengqing Liang, Keke ZhengAbstract:The intention of the study was to investigate the effect of ultrafiltered Fish Protein Hydrolysate (UF) level on growth, feed utilization, apparent digestibility coefficients and proximal intestine peptide transporter 1 (PepT1) mRNA level for juvenile turbot (Scophthalmus maximus L.). Experimental diets (UF-0, UF-5, UF-10, UF-15 and UF-20) were prepared containing about 68% plant Protein, and Fish meal Protein was, respectively, replaced by 0%, 5%, 10%, 15% and 20% UF of dietary Protein. Diet PP contained about 78% plant Protein, and diet CAA contained about 10% crystalline amino acid mixture. All diets were fed to seven triplicate groups of turbot (initial weight 16.05 ± 0.03 g) for 68 days. Fish fed diet UF-10 had an increasing tendency in growth compared with diets contained UF, while dietary UF level was not significantly correlated with specific growth rate and feed intake. Feed efficiency, Protein efficiency ratio and Protein productive value significantly correlated with dietary UF level, and Fish fed diets contained low-level UF had higher digestibility than that diets UF-0, PP and CAA. There was a decreasing tendency in PepT1 expression level with dietary UF level. The results indicated that low-level UF showed a positive effect on growth and feed utilization in juvenile turbot.
Eunice C Y Lichan - One of the best experts on this subject based on the ideXlab platform.
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neuroprotective effect of β secretase inhibitory peptide from pacific hake merluccius productus Fish Protein Hydrolysate
Current Alzheimer Research, 2019Co-Authors: Eunice C Y Lichan, Imelda W Y Cheung, Youjin Jeon, Juyoung Ko, Heeguk ByunAbstract:BACKGROUND: Various methodologies have been employed for the therapeutic interpolation of the progressive brain disorder Alzheimer's disease. Thus, β-secretase inhibition is significant to prevent disease progression in the early stages. OBJECTIVE: This study seeks to purify and characterize a novel β-secretase inhibitory peptide from Pacific hake enzymatic Hydrolysate. METHODS: A potent β-secretase inhibitory peptide was isolated by sequential purifications using Sephadex G-25 column chromatography and octadecylsilane (ODS) C18 reversed-phase HPLC. A total of seven peptides were synthesized using the isolated peptide sequences. SH-SY5Y cells stably transfected with the human ''Swedish'' amyloid precursor Protein (APP) mutation APP695 (SH-SY5YAPP695swe) were used as an in-vitro model system to investigate the effect of Leu-Asn peptide on APP processing. RESULTS: The β-secretase inhibitory activity (IC50) of the purified peptide (Ser-Leu-Ala-Phe-Val-Asp- Asp-Val-Leu-Asn) from Fish Protein Hydrolysate was 18.65 μM and dipeptide Leu-Asn was the most potent β-secretase inhibitor (IC50 value = 8.82 µM). When comparing all the seven peptides, the inhibition pattern of Leu-Asn dipeptide was found to be competitive by Lineweaver-Burk plot and Dixon plot (Ki value = 4.24 µM). The 24 h treatment with Leu-Asn peptide in SH-SY5Y cells resulted in reducing the β-amyloid (Aβ) production in a dose-dependent manner. CONCLUSION: Therefore, the results of this study suggest that β-secretase inhibitory peptides derived from marine organisms could be potential candidates to develop nutraceuticals or pharmaceuticals as antidementia agents.
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effects of Fish Protein Hydrolysate and freeze thaw treatment on physicochemical and gel properties of natural actomyosin from pacific cod
Food Chemistry, 2013Co-Authors: Malgorzata Korzeniowska, Imelda W Y Cheung, Eunice C Y LichanAbstract:Abstract The properties of natural actomyosin (NAM) containing 2% or 8% Fish Protein Hydrolysate (FPH-2, FPH-8) or 8% sucrose–sorbitol blend (SuSo) were compared to control NAM before and after freeze–thaw treatment. Surface hydrophobicity of control and FPH-2 increased after freeze–thaw treatment, while that of FPH-8 did not change, which may be related to greater thermostability of actin and myosin in FPH-8 as observed by differential scanning calorimetry. The cooked gel of freeze–thawed control had 39% expressible moisture after an 8.5% cook loss, whereas gels of freeze–thawed SuSo, FPH-2 and FPH-8 had significantly lower expressible moisture (15–22%) and no cook loss. Gels of freeze–thawed FPH-2 and FPH-8 were similar to unfrozen control gel in hardness, cohesiveness and gumminess. This study demonstrates that FPH effectively stabilised NAM Protein structure and function during freeze–thaw treatment.
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investigations into inhibitor type and mode simulated gastrointestinal digestion and cell transport of the angiotensin i converting enzyme inhibitory peptides in pacific hake merluccius productus fillet Hydrolysate
Journal of Agricultural and Food Chemistry, 2008Co-Authors: Crystal D Cinqmars, David D Kitts, Chun Hu, Eunice C Y LichanAbstract:Fish Protein Hydrolysate (FPH) produced by incubation of Pacific hake fillet with 3.00% Protamex at pH 6.5 and 40 °C for 125 min demonstrated in vitro ACE-inhibitory activity (IC50 = 165 µg/mL), which was enhanced by ultrafiltration through a 10 kDa molecular weight cutoff membrane (IC50 = 44 µg/mL). However, after simulated gastrointestinal digestion, FPH and ultrafiltrate had similar ACE-inhibitory activity (IC50 = 90 µg/mL), indicating that FPH peptides act as “pro-drug type” inhibitors and that enrichment by ultrafiltration may be unnecessary. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry confirmed that the molecular weights of major peaks were 0.05) and exhibited a competitive inhibitory mode. A permeability assay using fully differentiated colorectal adenocarcinoma (Caco-2) cells showed an apical to basolateral transpor...
Aniseh Jamshidi - One of the best experts on this subject based on the ideXlab platform.
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using wpc inulin fucoidan complexes for encapsulation of Fish Protein Hydrolysate and Fish oil in w1 o w2 emulsion characterization and nutritional quality
Food Research International, 2018Co-Authors: Aniseh Jamshidi, Bahareh Shabanpour, Parastoo Pourashouri, Mojtaba RaeisiAbstract:Abstract The double emulsions and freeze-dried microcapsules containing Fish Protein Hydrolysate (FPH) and Fish oil (FO) were stabilized by complexs of whey Protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release. Higher encapsulation efficiency and solubility were observed in Fuc-WPC microcapsules (86.31% and 30.26 mg/100 g, respectively). The combination of Fuc-WPC in the wall material showed higher oxidative stability than other wall material. The higher values of PUFA and SFA were observed in Inu-WPC Fuc-WPC microcapsules, respectively. The Fuc-WPC and Inu + Fuc-WPC micrographs showed a more porous structure compared to Inu-WPC. The mean particle size ranged from 536.8 ± 52.70 to 842.36 ± 21.41 nm. No significant differences were observed in the released oil and the fatty acid composition during gastrointestinal digestion. Sensory evaluation of fortified natural yogurt with microcapsules showed lower Fishy flavor in Inu-WPC samples than those fortified with Fuc-WPC and Inu + Fuc-WPC. In general, the use of inulin with WPC as a wall materal resulted in good characteristics and sensory attributed, although the use of fucoidan with WPC conferred higher oxidative stability during storage.
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Using WPC-inulin-fucoidan complexes for encapsulation of Fish Protein Hydrolysate and Fish oil in W1/O/W2 emulsion: Characterization and nutritional quality.
Food Research International, 2018Co-Authors: Aniseh Jamshidi, Bahareh Shabanpour, Parastoo Pourashouri, Mojtaba RaeisiAbstract:Abstract The double emulsions and freeze-dried microcapsules containing Fish Protein Hydrolysate (FPH) and Fish oil (FO) were stabilized by complexs of whey Protein concentrate (WPC) with inulin (Inu) and fucoidan (Fuc) in terms of physical characteristics (particle size distribution, morphology, encapsulation efficiency, solubility, …), oxidative stability, nutritional quality and in vitro release. Higher encapsulation efficiency and solubility were observed in Fuc-WPC microcapsules (86.31% and 30.26 mg/100 g, respectively). The combination of Fuc-WPC in the wall material showed higher oxidative stability than other wall material. The higher values of PUFA and SFA were observed in Inu-WPC Fuc-WPC microcapsules, respectively. The Fuc-WPC and Inu + Fuc-WPC micrographs showed a more porous structure compared to Inu-WPC. The mean particle size ranged from 536.8 ± 52.70 to 842.36 ± 21.41 nm. No significant differences were observed in the released oil and the fatty acid composition during gastrointestinal digestion. Sensory evaluation of fortified natural yogurt with microcapsules showed lower Fishy flavor in Inu-WPC samples than those fortified with Fuc-WPC and Inu + Fuc-WPC. In general, the use of inulin with WPC as a wall materal resulted in good characteristics and sensory attributed, although the use of fucoidan with WPC conferred higher oxidative stability during storage.
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Evaluation of Different Proportions of Distilled Water to Substrate on Functional Properties, Antioxidant and Nutritional Quality of Bigeye Ilisha (Ilisha Megaloptera) Protein Hydrolysate
Golestan University of Medical Sciences, 2018Co-Authors: Aniseh Jamshidi, Bahareh Shabanpour, Parastoo Pourashouri, Mojtaba RaeisiAbstract:Background and Objectives: Production of Fish Protein Hydrolysate is a method for converting the low-value economical underutilized Fish species to value-added products. This study aimed to evaluate the different proportions of distilled water to the substrate on the functional characteristics, antioxidant and nutritional quality of Fish Protein Hydrolysate of bigeye ilisha (Ilisha megaloptera) produced by enzymatic hydrolysis. Materials and Methods: After defatting of minced Fish, the hydrolysis process was carried out using three different 4:1, 5:1 and 6:1 distilled water to substrate proportions by using alcalase enzyme in three replications. The Protein Hydrolysate samples were analyzed for approximate composition (soluble Protein, moisture, fat and ash), functional characteristics (solubility, foam capacity, and foam stability), antioxidant properties (DPPH radical scavenging activity and reducing power) and mineral composition. Results: In this study, the ratio of distilled water to the substrate affected the Protein Hydrolysate properties and the highest amounts of hydrolysis degree and DPPH radical scavenging activity were observed in samples with 5:1 ratio. Fish Protein Hydrolysate obtained from 4:1 ratio had the highest amount of soluble Protein, and no significant difference was observed in term of solubility with samples obtained by 5:1 ratio. Moreover, there were no significant differences in terms of lightness and foam capacity of samples obtained from 4:1 and 6:1 proportions. Conclusion: According to the results of the study, using a 4:1 ratio of distilled water to substrate led to the production of Fish Protein Hydrolysates from bigeye ilisha with higher functional properties and nutritional composition
Mengqing Liang - One of the best experts on this subject based on the ideXlab platform.
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Fish Protein Hydrolysate affected amino acid absorption and related gene expressions of igf 1 akt pathways in turbot scophthalmus maximus
Aquaculture Nutrition, 2020Co-Authors: Yuliang Wei, Mengqing LiangAbstract:The effects of Fish Protein Hydrolysate (FPH) on growth, peptide and amino acid (AA) transporters, postprandial free AA and related gene expression of IGF‐1/AKT pathway were evaluated in turbot (Scophthalmus maximus). Three diets were formulated to contain the same low level of Fishmeal; meanwhile 0, 45 and 180 g/kg FPH were, respectively, supplemented to the FF (FPH‐free), FL (FPH‐Low) and FH (FPH‐High) diets. Fish fed the FH diet improved the growth compared with the other groups. For peptide and AA transporters, PepT1, B⁰AT1, CAT1 and PAT1 mRNA levels in proximal or distal intestine decreased in Fish fed the FH diet. The concentration of free total essential AAs in serum was higher in the FH treatment than that in the FF treatment at 2 and 6 hr after feeding. For IGF‐1/AKT pathway in muscle, IGF‐1, 4E‐BP1 and FoxO1 mRNA levels were the highest in the FH group, whereas IGF‐1R mRNA levels were the highest expression level in the FF group. In conclusion, down‐regulated AAs transport, alleviated the delayed postprandial peak of serum‐free AAs and increased muscle Protein synthesis were observed to improve the growth when turbot was fed high FPH level diets containing a high plant Protein.
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Fish Protein Hydrolysate affected amino acid absorption and related gene expressions of IGF‐1/AKT pathways in turbot (Scophthalmus maximus)
Aquaculture Nutrition, 2019Co-Authors: Yuliang Wei, Mengqing LiangAbstract:The effects of Fish Protein Hydrolysate (FPH) on growth, peptide and amino acid (AA) transporters, postprandial free AA and related gene expression of IGF‐1/AKT pathway were evaluated in turbot (Scophthalmus maximus). Three diets were formulated to contain the same low level of Fishmeal; meanwhile 0, 45 and 180 g/kg FPH were, respectively, supplemented to the FF (FPH‐free), FL (FPH‐Low) and FH (FPH‐High) diets. Fish fed the FH diet improved the growth compared with the other groups. For peptide and AA transporters, PepT1, B⁰AT1, CAT1 and PAT1 mRNA levels in proximal or distal intestine decreased in Fish fed the FH diet. The concentration of free total essential AAs in serum was higher in the FH treatment than that in the FF treatment at 2 and 6 hr after feeding. For IGF‐1/AKT pathway in muscle, IGF‐1, 4E‐BP1 and FoxO1 mRNA levels were the highest in the FH group, whereas IGF‐1R mRNA levels were the highest expression level in the FF group. In conclusion, down‐regulated AAs transport, alleviated the delayed postprandial peak of serum‐free AAs and increased muscle Protein synthesis were observed to improve the growth when turbot was fed high FPH level diets containing a high plant Protein.
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1h nmr based metabolomics studies on the effect of size fractionated Fish Protein Hydrolysate Fish meal and plant Protein in diet for juvenile turbot scophthalmus maximus l
Aquaculture Nutrition, 2017Co-Authors: Yuliang Wei, Mengqing Liang, Kangsen Mai, Keke ZhengAbstract:This study was designed to evaluate changes in the metabolic profile of liver and muscle of turbot (Scophthalmus maximus L.) fed Fishmeal-based diet, diets containing size-fractionated Fish Protein Hydrolysate and plant Protein-based diet using 1H NMR-based metabolomics approach combined with the growth. Fish Protein Hydrolysate (FPH) was obtained by enzymatic treatment, permeate fraction was obtained as UF by ultrafiltered step, and retentate fraction was retained as RF. FM diet contained Fish meal used as a single Protein source. Four other diets (PP, UF, FPH and RF) contained 180 g kg−1 diet Fish meal. 54, 55 and 55 g kg−1 dry diet UF, FPH and RF were supplemented to UF, FPH and RF diets. All diets were formulated to be isolipidic and isonitrogenous fed to five triplicate groups of turbot (16.05 ± 0.03 g) for 68 days. O-PLS-DA in FM versus UF, FM versus FPH, FM versus RF and FM versus PP resulted in a reliable model for muscle and liver tissue, while O-PLS-DA in UF versus FPH and UF versus RF only showed metabolites changes in liver tissue. Results indicated that metabolite changes among the different treatments were consistent with the growth tendency.
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The effect of ultrafiltered Fish Protein Hydrolysate levels on the liver and muscle metabolic profile of juvenile turbot (Scophthalmus maximus L.) by 1H NMR‐based metabolomics studies
Aquaculture Research, 2016Co-Authors: Yuliang Wei, Mengqing Liang, Kangsen Mai, Keke ZhengAbstract:A 1H NMR-based metabolomics approach was to explore the effect of ultrafiltered Fish Protein Hydrolysate (UF) levels on the liver and muscle metabolic profile of juvenile turbot (Scophthalmus maximus L.). Fish Protein Hydrolysate (FPH) was produced from by-products by enzymatic treatment, and UF was obtained by diluting FPH followed filtration. Fish were fed diets containing Fish meal Protein, which was, respectively, replaced by UF Protein 0, 50, 100, 150 and 200 g kg−1 of dietary total Protein (UF-0, UF-5, UF-10, UF-15 and UF-20) for 68 days. OPLS-DA of liver and muscle showed that high levels of UF in diets may lead to corresponding tissue metabolites changes. In liver tissue, changing metabolites included dimethylamine, N,N-dimethylglycine, valine, isoleucine, leucine, arginine and alanine and were mainly involved in choline metabolism and amino acid metabolism. For muscle tissue, changing metabolites included lactate, alanine, proline, fumarate, tyrosine, histidine, cystathionine and taurine. And the metabolites were mainly involved in glycolysis and gluconeogenesis, proline metabolism and taurine metabolism. 1H NMR-based metabolomics is a useful approach to investigate different levels of UF on metabolic profile in liver and muscle tissues combined with the growth.
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1H NMR‐based metabolomics studies on the effect of size‐fractionated Fish Protein Hydrolysate, Fish meal and plant Protein in diet for juvenile turbot (Scophthalmus maximus L.)
Aquaculture Nutrition, 2016Co-Authors: Yuliang Wei, Mengqing Liang, Kangsen Mai, Keke ZhengAbstract:This study was designed to evaluate changes in the metabolic profile of liver and muscle of turbot (Scophthalmus maximus L.) fed Fishmeal-based diet, diets containing size-fractionated Fish Protein Hydrolysate and plant Protein-based diet using 1H NMR-based metabolomics approach combined with the growth. Fish Protein Hydrolysate (FPH) was obtained by enzymatic treatment, permeate fraction was obtained as UF by ultrafiltered step, and retentate fraction was retained as RF. FM diet contained Fish meal used as a single Protein source. Four other diets (PP, UF, FPH and RF) contained 180 g kg−1 diet Fish meal. 54, 55 and 55 g kg−1 dry diet UF, FPH and RF were supplemented to UF, FPH and RF diets. All diets were formulated to be isolipidic and isonitrogenous fed to five triplicate groups of turbot (16.05 ± 0.03 g) for 68 days. O-PLS-DA in FM versus UF, FM versus FPH, FM versus RF and FM versus PP resulted in a reliable model for muscle and liver tissue, while O-PLS-DA in UF versus FPH and UF versus RF only showed metabolites changes in liver tissue. Results indicated that metabolite changes among the different treatments were consistent with the growth tendency.