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Guodong Wang - One of the best experts on this subject based on the ideXlab platform.

  • a class b heat shock factor selected for during soybean domestication contributes to salt tolerance by promoting Flavonoid Biosynthesis
    New Phytologist, 2020
    Co-Authors: Xiaohua Bian, Canfang Niu, Wei Wei, Jiaqi Han, Jianjun Tao, Meng Jin, Hao Qin, Bin Zhou, Wanke Zhang, Guodong Wang
    Abstract:

    Soybean (Glycine max) production is severely affected in unfavorable environments. Identification of the regulatory factors conferring stress tolerance would facilitate soybean breeding. In this study, through coexpression network analysis of salt-tolerant wild soybeans, together with molecular and genetic approaches, we revealed a previously unidentified function of a class B heat shock factor, HSFB2b, in soybean salt stress response. We showed that HSFB2b improves salt tolerance through the promotion of Flavonoid accumulation by activating one subset of Flavonoid Biosynthesis-related genes and by inhibiting the repressor gene GmNAC2 to release another subset of genes in the Flavonoid Biosynthesis pathway. Moreover, four promoter haplotypes of HSFB2b were identified from wild and cultivated soybeans. Promoter haplotype II from salt-tolerant wild soybean Y20, with high promoter activity under salt stress, is probably selected for during domestication. Another promoter haplotype, III, from salt-tolerant wild soybean Y55, had the highest promoter activity under salt stress, had a low distribution frequency and may be subjected to the next wave of selection. Together, our results revealed the mechanism of HSFB2b in soybean salt stress tolerance. Its promoter variations were identified, and the haplotype with high activity may be adopted for breeding better soybean cultivars that are adapted to stress conditions.

  • noncatalytic chalcone isomerase fold proteins in humulus lupulus are auxiliary components in prenylated Flavonoid Biosynthesis
    Proceedings of the National Academy of Sciences of the United States of America, 2018
    Co-Authors: A J Mitchell, Fengxia Zhang, Jingke Weng, Guodong Wang
    Abstract:

    Xanthohumol (XN) and demethylxanthohumol (DMX) are specialized prenylated chalconoids with multiple pharmaceutical applications that accumulate to high levels in the glandular trichomes of hops (Humulus lupulus L.). Although all structural enzymes in the XN pathway have been functionally identified, biochemical mechanisms underlying highly efficient production of XN have not been fully resolved. In this study, we characterized two noncatalytic chalcone isomerase (CHI)-like proteins (designated as HlCHIL1 and HlCHIL2) using engineered yeast harboring all genes required for DMX production. HlCHIL2 increased DMX production by 2.3-fold, whereas HlCHIL1 significantly decreased DMX production by 30%. We show that CHIL2 is part of an active DMX biosynthetic metabolon in hop glandular trichomes that encompasses a chalcone synthase (CHS) and a membrane-bound prenyltransferase, and that type IV CHI-fold proteins of representative land plants contain conserved function to bind with CHS and enhance its activity. Binding assays and structural docking uncover a function of HlCHIL1 to bind DMX and naringenin chalcone to stabilize the ring-open configuration of these chalconoids. This study reveals the role of two HlCHILs in DMX Biosynthesis in hops, and provides insight into their evolutionary development from the ancestral fatty acid-binding CHI-fold proteins to specialized auxiliary proteins supporting Flavonoid Biosynthesis in plants.

Xiaochun Wan - One of the best experts on this subject based on the ideXlab platform.

  • metabolite profiling and transcriptomic analyses reveal an essential role of uvr8 mediated signal transduction pathway in regulating Flavonoid Biosynthesis in tea plants camellia sinensis in response to shading
    BMC Plant Biology, 2018
    Co-Authors: Linlin Liu, Jian Zhao, Brian R Jordan, Guangbiao She, Xianchen Zhang, Qi Chen, Xiaochun Wan
    Abstract:

    Tea is the most popular nonalcoholic beverage worldwide for its pleasant characteristics and healthful properties. Catechins, theanine and caffeine are the major natural products in tea buds and leaves that determine tea qualities such as infusion colors, tastes and fragrances, as well as their health benefits. Shading is a traditional and effective practice to modify natural product accumulation and to enhance the tea quality in tea plantation. However, the mechanism underlying the shading effects is not fully understood. This study aims to explore the regulation of Flavonoid Biosynthesis in Camellia sinensis under shading by using both metabolomic and transcriptional analyses. While shading enhanced chlorophyll accumulation, major catechins, including C, EC, GC and EGC, decreased significantly in tea buds throughout the whole shading period. The reduction of catechins and flavonols were consistent with the simultaneous down-regulation of biosynthetic genes and TFs associated with Flavonoid Biosynthesis. Of 16 genes involved in the Flavonoid biosynthetic pathway, F3’H and FLS significantly decreased throughout shading while the others (PAL, CHSs, DFR, ANS, ANR and LAR, etc.) temporally decreased in early or late shading stages. Gene co-expression cluster analysis suggested that a number of photoreceptors and potential genes involved in UV-B signal transductions (UVR8_L, HY5, COP1 and RUP1/2) showed decreasing expression patterns consistent with structural genes (F3’H, FLS, ANS, ANR, LAR, DFR and CHSs) and potential TFs (MYB4, MYB12, MYB14 and MYB111) involved in Flavonoid Biosynthesis, when compared with genes in the UV-A/blue and red/far-red light signal transductions. The KEGG enrichment and matrix correlation analyses also attributed the regulation of catechin Biosynthesis to the UVR8-mediated signal transduction pathway. Further UV-B treatment in the controlled environment confirmed UV-B induction on flavonols and EGCG accumulation in tea leaves. We proposed that catechin Biosynthesis in C. sinensis leaves is predominantly regulated by UV through the UVR8-mediated signal transduction pathway to MYB12/MYB4 downstream effectors, to modulate Flavonoid accumulation. Our study provides new insights into our understanding of regulatory mechanisms for shading-enhanced tea quality.

  • Metabolite profiling and transcriptomic analyses reveal an essential role of UVR8-mediated signal transduction pathway in regulating Flavonoid Biosynthesis in tea plants (Camellia sinensis) in response to shading
    BMC, 2018
    Co-Authors: Linlin Liu, Jian Zhao, Guangbiao She, Xianchen Zhang, Brian Jordan, Qi Chen, Xiaochun Wan
    Abstract:

    Abstract Background Tea is the most popular nonalcoholic beverage worldwide for its pleasant characteristics and healthful properties. Catechins, theanine and caffeine are the major natural products in tea buds and leaves that determine tea qualities such as infusion colors, tastes and fragrances, as well as their health benefits. Shading is a traditional and effective practice to modify natural product accumulation and to enhance the tea quality in tea plantation. However, the mechanism underlying the shading effects is not fully understood. This study aims to explore the regulation of Flavonoid Biosynthesis in Camellia sinensis under shading by using both metabolomic and transcriptional analyses. Results While shading enhanced chlorophyll accumulation, major catechins, including C, EC, GC and EGC, decreased significantly in tea buds throughout the whole shading period. The reduction of catechins and flavonols were consistent with the simultaneous down-regulation of biosynthetic genes and TFs associated with Flavonoid Biosynthesis. Of 16 genes involved in the Flavonoid biosynthetic pathway, F3’H and FLS significantly decreased throughout shading while the others (PAL, CHSs, DFR, ANS, ANR and LAR, etc.) temporally decreased in early or late shading stages. Gene co-expression cluster analysis suggested that a number of photoreceptors and potential genes involved in UV-B signal transductions (UVR8_L, HY5, COP1 and RUP1/2) showed decreasing expression patterns consistent with structural genes (F3’H, FLS, ANS, ANR, LAR, DFR and CHSs) and potential TFs (MYB4, MYB12, MYB14 and MYB111) involved in Flavonoid Biosynthesis, when compared with genes in the UV-A/blue and red/far-red light signal transductions. The KEGG enrichment and matrix correlation analyses also attributed the regulation of catechin Biosynthesis to the UVR8-mediated signal transduction pathway. Further UV-B treatment in the controlled environment confirmed UV-B induction on flavonols and EGCG accumulation in tea leaves. Conclusions We proposed that catechin Biosynthesis in C. sinensis leaves is predominantly regulated by UV through the UVR8-mediated signal transduction pathway to MYB12/MYB4 downstream effectors, to modulate Flavonoid accumulation. Our study provides new insights into our understanding of regulatory mechanisms for shading-enhanced tea quality

  • the r2r3 myb bhlh wd40 and related transcription factors in Flavonoid Biosynthesis
    Functional & Integrative Genomics, 2013
    Co-Authors: Lei Zhao, Xiaochun Wan, Liping Gao, Hongxue Wang, Xiaotian Chen, Yunsheng Wang, Hua Yang, Chaoling Wei, Tao Xia
    Abstract:

    R2R3-MYB, bHLH, and WD40 proteins have been shown to control multiple enzymatic steps in the biosynthetic pathway responsible for the production of Flavonoids, important secondary metabolites in Camellia sinensis. Few related transcription factor genes have been documented. The presence of R2R3-MYB, bHLH, and WD40 were statistically and bioinformatically analyzed on 127,094 C. sinensis transcriptome unigenes, resulting in identification of 73, 49, and 134 genes, respectively. C. sinensis phylogenetic trees were constructed for R2R3-MYB and bHLH proteins using previous Arabidopsis data and further divided into 27 subgroups (Sg) and 32 subfamilies. Motifs in some R2R3-MYB subgroups were redefined. Furthermore, Sg26 and Sg27 were expanded compared to Arabidopsis data, and bHLH proteins in C. sinensis were grouped into nine subfamilies. According to the functional annotation of Arabidopsis, Flavonoid Biosynthesis in C. sinensis was predicted to include R2R3-MYB genes in Sg4 (6), Sg5 (2), and Sg7 (1), as well as bHLH genes in subfamily 2 (2) and subfamily 24 (5). The wide evolutionary gap prevented phylogenetic analysis of WD40s; however, a single gene, CsWD40-1, was observed to share 80.4 % sequence homogeny with AtTTG1. Analysis of CsMYB4-1, CsMYB4-2, CsMYB4-3, CsMYB4-4, CsMYB5-1, and CsMYB5-2 revealed the interaction motif [DE]Lx2[RK]x3Lx6Lx3R, potentially contributing to the specificity of the bHLH partner in the stable MYB–bHLH complex. Full-length end-to-end polymerase chain reaction (PCR) and quantitative reverse transcriptase (qRT)-PCR were used to validate selected genes and generate relative expression ratio profiles in C. sinensis leaves by developmental stage and treatment conditions, including hormone and wound treatments. Potential target binding sites were predicted.

Lei Wang - One of the best experts on this subject based on the ideXlab platform.

  • a novel dark septate fungal endophyte positively affected blueberry growth and changed the expression of plant genes involved in phytohormone and Flavonoid Biosynthesis
    Tree Physiology, 2020
    Co-Authors: Wei Tian, Yadong Sun, Feiyan Chen, Yurou Zhang, Yuxuan Zhai, Jing Zhang, Lei Wang
    Abstract:

    Dark septate endophytes (DSEs) are one of the most studied groups of root fungal endophytes in recent years. However, the effects of DSE on host plant are still under debate, and the molecular mechanisms are poorly understood. In this study, we identified a DSE fungus of the genus Anteaglonium, named T010, from the wild blueberry. When inoculated into Vaccinium corymbosum L. plants, T010 could enhance root growth and promote shoot branching, leading to increased plant growth. By comparative transcriptome analysis, we obtained 1948 regulated differentially expressed genes (DEGs) from the V. corymbosum plants treated by T010. Further functional enrichment analysis identified a series of DEGs enriched in transcriptional regulation, material transport, phytohormone Biosynthesis and Flavonoid Biosynthesis. Moreover, the comparative analysis of liquid chromatography-mass spectrometry verified that T010 treatment induced the changes in the contents of various phytohormones and Flavonoids. This is the first report on the isolation of DSE fungi of the genus Anteaglonium from blueberry roots. Moreover, our results suggested that T010 colonization could result in a series of changes in cell metabolism, Biosynthesis and signal pathways, thereby promoting plant growth. Particularly, the changes of phytohormone and Flavonoid metabolism induced by T010 colonization might contribute to the promotion of blueberry growth. Our results will provide new insights into understanding of the interaction of DSE fungi and host plants, as well as the development and utilization of DSE preparations.

  • a novel dark septate fungal endophyte positively affected blueberry growth and changed the expression of plant genes involved in phytohormone and Flavonoid Biosynthesis
    Tree Physiology, 2020
    Co-Authors: Fanlin Wu, Wei Tian, Feiyan Chen, Yurou Zhang, Yuxuan Zhai, Jing Zhang, Yan Li, Hongyan Su, Lei Wang
    Abstract:

    : Dark septate endophytes (DSEs) are one of the most studied groups of root fungal endophytes in recent years. However, the effects of DSE on host plant are still under debate and the molecular mechanisms are poorly understood. In this study, we identified a DSE fungus of the genus Anteaglonium, named T010, from the wild blueberry. When inoculated into Vaccinium corymbosum plants, T010 could enhance root growth and promote shoot branching, leading to increased plant growth. By comparative transcriptome analysis, we obtained 1,948 regulated differentially expressed genes (DEGs) from the V. corymbosum plants treated by T010. Further functional enrichment analysis identified a series of DEGs enriched in transcriptional regulation, material transport, phytohormone Biosynthesis, and Flavonoid Biosynthesis. Moreover, the comparative analysis of LC-MS verified that T010 treatment induced the changes in the contents of various phytohormones and Flavonoids. This is the first report on the isolation of DSE fungi of the genus Anteaglonium from blueberry roots. Moreover, our results suggested that T010 colonization could result in a series of changes in cell metabolism, Biosynthesis and signal pathways, thereby promoting plants growth. Particularly, the changes of phytohormone and Flavonoid metabolism induced by T010 colonization might contribute to the promotion of blueberry growth. Our results will provide new insights into understanding of the interaction of DSE fungi and host plants, as well as the development and utilization of DSE preparations.

Songling Bai - One of the best experts on this subject based on the ideXlab platform.

  • ethylene mediates the branching of the jasmonate induced Flavonoid Biosynthesis pathway by suppressing anthocyanin Biosynthesis in red chinese pear fruits
    Plant Biotechnology Journal, 2020
    Co-Authors: Yuan Zhao, Ruiyan Tao, Lei Yin, Ling Gao, Ake Strid, Minjie Qian, Jiaqi Shen, Yuanwen Teng, Songling Bai
    Abstract:

    Flavonoid accumulation in most fruits is enhanced by ethylene and jasmonate. However, little is known about the hormone functions related to red pear fruit coloration or their combined effects and potential underlying mechanisms. Various treatments were used to investigate the Flavonoid metabolite profile and pear transcriptome to verify the effects of ethylene and jasmonate on Flavonoid Biosynthesis in red pear fruits as well as the mechanism behind this. Ethylene inhibits anthocyanin Biosynthesis in red Chinese pear fruits, whereas jasmonate increases anthocyanin and flavone/isoflavone biosyntheses. The branching of the jasmonate-induced Flavonoid Biosynthesis pathway is determined by ethylene. Co-expression network and Mfuzz analyses revealed 4,368 candidate transcripts. Additionally, ethylene suppresses PpMYB10 and PpMYB114 expression via TF repressors, ultimately decreasing anthocyanin Biosynthesis. Jasmonate induces anthocyanin accumulation through transcriptional or post-translational regulation of TFs-like MYB and bHLH in the absence of ethylene. However, jasmonate induces ethylene Biosynthesis and the associated signalling pathway in pear, thereby decreasing anthocyanin production, increasing the availability of the precursors for flavone/isoflavone Biosynthesis and enhancing deep yellow fruit coloration. We herein present new phenotypes and fruit coloration regulatory patterns controlled by jasmonate and ethylene, and confirm that the regulation of fruit coloration is complex.

Andrew C Allan - One of the best experts on this subject based on the ideXlab platform.

  • transcriptional regulation of Flavonoid Biosynthesis in nectarine prunus persica by a set of r2r3 myb transcription factors
    BMC Plant Biology, 2013
    Co-Authors: Daniela Ravaglia, Richard V Espley, Rebecca A Henrykirk, Carlo Andreotti, Vanina Ziosi, Roger P Hellens, Guglielmo Costa, Andrew C Allan
    Abstract:

    Flavonoids such as anthocyanins, flavonols and proanthocyanidins, play a central role in fruit colour, flavour and health attributes. In peach and nectarine (Prunus persica) these compounds vary during fruit growth and ripening. Flavonoids are produced by a well studied pathway which is transcriptionally regulated by members of the MYB and bHLH transcription factor families. We have isolated nectarine Flavonoid regulating genes and examined their expression patterns, which suggests a critical role in the regulation of Flavonoid Biosynthesis. In nectarine, expression of the genes encoding enzymes of the Flavonoid pathway correlated with the concentration of proanthocyanidins, which strongly increases at mid-development. In contrast, the only gene which showed a similar pattern to anthocyanin concentration was UDP-glucose-Flavonoid-3-O-glucosyltransferase (UFGT), which was high at the beginning and end of fruit growth, remaining low during the other developmental stages. Expression of flavonol synthase (FLS1) correlated with flavonol levels, both temporally and in a tissue specific manner. The pattern of UFGT gene expression may be explained by the involvement of different transcription factors, which up-regulate Flavonoid Biosynthesis (MYB10, MYB123, and bHLH3), or repress (MYB111 and MYB16) the transcription of the biosynthetic genes. The expression of a potential proanthocyanidin-regulating transcription factor, MYBPA1, corresponded with proanthocyanidin levels. Functional assays of these transcription factors were used to test the specificity for Flavonoid regulation. MYB10 positively regulates the promoters of UFGT and dihydroflavonol 4-reductase (DFR) but not leucoanthocyanidin reductase (LAR). In contrast, MYBPA1 trans-activates the promoters of DFR and LAR, but not UFGT. This suggests exclusive roles of anthocyanin regulation by MYB10 and proanthocyanidin regulation by MYBPA1. Further, these transcription factors appeared to be responsive to both developmental and environmental stimuli.

  • transcriptional regulation of Flavonoid Biosynthesis in nectarine prunus persica by a set of r2r3 myb transcription factors
    Science & Engineering Faculty, 2013
    Co-Authors: Daniela Ravaglia, Richard V Espley, Rebecca A Henrykirk, Carlo Andreotti, Vanina Ziosi, Roger P Hellens, Guglielmo Costa, Andrew C Allan
    Abstract:

    Background Flavonoids such as anthocyanins, flavonols and proanthocyanidins, play a central role in fruit colour, flavour and health attributes. In peach and nectarine (Prunus persica) these compounds vary during fruit growth and ripening. Flavonoids are produced by a well studied pathway which is transcriptionally regulated by members of the MYB and bHLH transcription factor families. We have isolated nectarine Flavonoid regulating genes and examined their expression patterns, which suggests a critical role in the regulation of Flavonoid Biosynthesis. Results In nectarine, expression of the genes encoding enzymes of the Flavonoid pathway correlated with the concentration of proanthocyanidins, which strongly increases at mid-development. In contrast, the only gene which showed a similar pattern to anthocyanin concentration was UDP-glucose-Flavonoid-3-O-glucosyltransferase (UFGT), which was high at the beginning and end of fruit growth, remaining low during the other developmental stages. Expression of flavonol synthase (FLS1) correlated with flavonol levels, both temporally and in a tissue specific manner. The pattern of UFGT gene expression may be explained by the involvement of different transcription factors, which up-regulate Flavonoid Biosynthesis (MYB10, MYB123, and bHLH3), or repress (MYB111 and MYB16) the transcription of the biosynthetic genes. The expression of a potential proanthocyanidin-regulating transcription factor, MYBPA1, corresponded with proanthocyanidin levels. Functional assays of these transcription factors were used to test the specificity for Flavonoid regulation. Conclusions MYB10 positively regulates the promoters of UFGT and dihydroflavonol 4-reductase (DFR) but not leucoanthocyanidin reductase (LAR). In contrast, MYBPA1 trans-activates the promoters of DFR and LAR, but not UFGT. This suggests exclusive roles of anthocyanin regulation by MYB10 and proanthocyanidin regulation by MYBPA1. Further, these transcription factors appeared to be responsive to both developmental and environmental stimuli.