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Mohamed Abdelkawy - One of the best experts on this subject based on the ideXlab platform.

  • spectrofluorimetric determination of 3 methylflavone 8 carboxylic acid the main active metabolite of Flavoxate hydrochloride in human urine
    Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2015
    Co-Authors: Hala E Zaazaa, Afaf O Mohamed, Maha A Hawwam, Mohamed Abdelkawy
    Abstract:

    A simple, sensitive and selective spectrofluorimetric method has been developed for the determination of 3-methylflavone-8-carboxylic acid as the main active metabolite of Flavoxate hydrochloride in human urine. The proposed method was based on the measurement of the native fluorescence of the metabolite in methanol at an emission wavelength 390 nm, upon excitation at 338 nm. Moreover, the urinary excretion pattern has been calculated using the proposed method. Taking the advantage that 3-methylflavone-8-carboxylic acid is also the alkaline degradate, the proposed method was applied to in vitro determination of Flavoxate hydrochloride in tablets dosage form via the measurement of its corresponding degradate. The method was validated in accordance with the ICH requirements and statistically compared to the official method with no significant difference in performance.

Kimio Sugaya - One of the best experts on this subject based on the ideXlab platform.

  • intravenous or local injections of Flavoxate in the rostral pontine reticular formation inhibit urinary frequency induced by activation of medial frontal lobe neurons in rats
    The Journal of Urology, 2014
    Co-Authors: Kimio Sugaya, Saori Nishijima, Katsumi Kadekawa, Katsuhiro Ashitomi, Tomoyuki Ueda, Hideyuki Yamamoto
    Abstract:

    Purpose: The rostral pontine reticular formation has a strong inhibitory effect on micturition by facilitating lumbosacral glycinergic neurons. We assessed the influence of the rostral pontine reticular formation on the micturition reflex after noradrenaline injection in the medial frontal lobe. We also examined the relation between the medial frontal lobe and the rostral pontine reticular formation.Materials and Methods: Continuous cystometry was performed in 28 female rats. After the interval between bladder contractions was shortened by noradrenaline injection in the medial frontal lobe we injected glutamate or Flavoxate hydrochloride in the rostral pontine reticular formation or intravenously injected Flavoxate or propiverine. The change in bladder activity was examined.Results: Noradrenaline injection in the medial frontal lobe shortened the interval between bladder contractions. In contrast to the bladder contraction interval before and after noradrenaline injection in the medial frontal lobe, the i...

  • activation of the rostral pontine reticular formation increases the spinal glycine level and inhibits bladder contraction in rats
    The Journal of Urology, 2005
    Co-Authors: Saori Nishijima, Minoru Miyazato, Kimio Sugaya, Shuichi Shimabukuro, Makoto Morozumi, Yoshihide Ogawa
    Abstract:

    ABSTRACT Purpose: We examined the mechanism involved in the inhibition of bladder activity in rats by stimulating the rostral pontine reticular formation (RPRF) using carbachol, Flavoxate and propiverine, and by analysis of amino acid levels in the lumbosacral cord. Materials and Methods: A total of 82 female rats were anesthetized with urethane. Under isovolumetric conditions physiological saline, carbachol, Flavoxate or propiverine was injected into the RPRF or intravenously. Changes in bladder activity and amino acid levels in the lumbosacral cord were examined. Results: Injection of carbachol or Flavoxate (0.3 μM each) into the RPRF abolished bladder contraction but there was no change after injection of physiological saline or propiverine. Intravenous injection of Flavoxate or propiverine (0.1 to 10 mg/kg each) inhibited bladder contraction. Amino acid analysis revealed that injection of carbachol into the RPRF increased glutamate and glycine levels in the lumbosacral cord, while injection of Flavoxate into the RPRF or intravenously caused an increase in glycine the lumbosacral cord. Injection of propiverine into the RPRF or intravenously did not influence lumbosacral cord amino acid levels. Conclusions: These results suggest that the RPRF has an important role in the inhibition of bladder contraction and carbachol or Flavoxate can activate descending RPRF neurons and inhibit bladder contraction via spinal glycinergic neurons.

  • Mechanisms of the Suppression of the Bladder Activity by Flavoxate
    International journal of urology : official journal of the Japanese Urological Association, 1996
    Co-Authors: Yutaka Kimura, Kimio Sugaya, Yasuo Sasaki, Kozo Hamada, Yojiro Ukai, Yoshiaki Yoshikuni, Kiyoshi Kimura, Hisashi Fukui, Osamu Nishizawa
    Abstract:

    Background: This study was designed to clarify the primary site of action of Flavoxate, clinically used for the treatment of urinary frequency. Methods: In rats, the effect of Flavoxate on contractile responses in isolated detrusor strips, bladder contraction induced by pelvic nerve stimulation, isovolumetric rhythmic bladder contractions, and pelvic nerve activity were examined. In decerebrated cats, Flavoxate was microinjected into the nuclei in the pons, and its effect on reflex micturition was observed. Results: Flavoxate suppressed carbachol- and calcium ion (Ca2+)-induced contractions of isolated detrusor strips in a noncompetitive and a competitive manner, respectively. Intravenous Flavoxate suppressed both initial phasic, and later tonic, bladder contractions induced by electrical stimulation of the distal end of the pelvic nerve. It abolished isovolumetric rhythmic bladder contractions and the associated efferent pelvic nerve activity, without affecting baseline vesical pressure and afferent pelvic nerve activity. When administered intracerebroventricularly or intrathecally, it abolished isovolumetric rhythmic bladder contractions. Flavoxate microinjected into the nucleus reticularis pontis oralis (PoO; pontine micturition inhibitory region) of decerebrated cats inhibited the reflex micturition, but had no effect when microinjected into the locus coeruleus alpha (pontine micturition center) or locus subcoeruleus (pontine urine storage center). Conclusions: Flavoxate suppressed the micturition reflex primarily by facilitating the inhibitory action of the PoO on the descending pathways from the pontine micturition center to the sacral arasympathetic intermediolateral nuclei.

Randa A Abdelsalam - One of the best experts on this subject based on the ideXlab platform.

  • high performance liquid chromatographic determination of Flavoxate hydrochloride and its hydrolysis product
    Drug Development and Industrial Pharmacy, 2008
    Co-Authors: Alaa Elgindy, Randa A Abdelsalam, Shehab A Sallam
    Abstract:

    Liquid chromatographic method was presented for the determination of Flavoxate hydrochloride (FX) and its hydrolysis product. The method was based on high-performance liquid chromatographic (HPLC) separation of FX from its hydrolysis product on CN column using a mobile phase consisting of acetonitrile-12 mM ammonium acetate (45:55, vol/vol, pH 4.0) with UV detection at 220 nm and flow rate of 1.5 mL min−1. The proposed HPLC method for the determination of FX was utilized to investigate the kinetics of acidic hydrolytic process at different temperatures and to calculate its activation energy. In addition, the proposed HPLC method was used for pH-rate profile study of hydrolysis of FX in Britton–Robinson buffer solutions. The 3-methylflavone-8-carboxylic acid ethyl ester, as impurity of Flavoxate hydrochloride, can be separated by the proposed HPLC method.

  • high performance liquid chromatographic determination of 3 methylflavone 8 carboxylic acid the main active metabolite of Flavoxate hydrochloride in human urine
    Journal of Pharmaceutical and Biomedical Analysis, 2007
    Co-Authors: Alaa Elgindy, Shehab A Sallam, Randa A Abdelsalam
    Abstract:

    High performance liquid chromatographic (HPLC) method was presented for the determination of 3-methylflavone-8-carboxylic acid as the main active metabolite of Flavoxate hydrochloride (FX) in human urine. The proposed method was based on using CN column with mobile phase consisting of acetonitrile-12 mM ammonium acetate (40:60, v/v) and adjusted to apparent pH 4.0 with flow rate of 1.5 ml min(-1). Quantitation was achieved with UV detection at 220 nm. The proposed method was utilized to the determination of dissolution rate for tablets containing Flavoxate hydrochloride. The urinary excretion pattern has been calculated using the proposed method.

Saori Nishijima - One of the best experts on this subject based on the ideXlab platform.

  • intravenous or local injections of Flavoxate in the rostral pontine reticular formation inhibit urinary frequency induced by activation of medial frontal lobe neurons in rats
    The Journal of Urology, 2014
    Co-Authors: Kimio Sugaya, Saori Nishijima, Katsumi Kadekawa, Katsuhiro Ashitomi, Tomoyuki Ueda, Hideyuki Yamamoto
    Abstract:

    Purpose: The rostral pontine reticular formation has a strong inhibitory effect on micturition by facilitating lumbosacral glycinergic neurons. We assessed the influence of the rostral pontine reticular formation on the micturition reflex after noradrenaline injection in the medial frontal lobe. We also examined the relation between the medial frontal lobe and the rostral pontine reticular formation.Materials and Methods: Continuous cystometry was performed in 28 female rats. After the interval between bladder contractions was shortened by noradrenaline injection in the medial frontal lobe we injected glutamate or Flavoxate hydrochloride in the rostral pontine reticular formation or intravenously injected Flavoxate or propiverine. The change in bladder activity was examined.Results: Noradrenaline injection in the medial frontal lobe shortened the interval between bladder contractions. In contrast to the bladder contraction interval before and after noradrenaline injection in the medial frontal lobe, the i...

  • activation of the rostral pontine reticular formation increases the spinal glycine level and inhibits bladder contraction in rats
    The Journal of Urology, 2005
    Co-Authors: Saori Nishijima, Minoru Miyazato, Kimio Sugaya, Shuichi Shimabukuro, Makoto Morozumi, Yoshihide Ogawa
    Abstract:

    ABSTRACT Purpose: We examined the mechanism involved in the inhibition of bladder activity in rats by stimulating the rostral pontine reticular formation (RPRF) using carbachol, Flavoxate and propiverine, and by analysis of amino acid levels in the lumbosacral cord. Materials and Methods: A total of 82 female rats were anesthetized with urethane. Under isovolumetric conditions physiological saline, carbachol, Flavoxate or propiverine was injected into the RPRF or intravenously. Changes in bladder activity and amino acid levels in the lumbosacral cord were examined. Results: Injection of carbachol or Flavoxate (0.3 μM each) into the RPRF abolished bladder contraction but there was no change after injection of physiological saline or propiverine. Intravenous injection of Flavoxate or propiverine (0.1 to 10 mg/kg each) inhibited bladder contraction. Amino acid analysis revealed that injection of carbachol into the RPRF increased glutamate and glycine levels in the lumbosacral cord, while injection of Flavoxate into the RPRF or intravenously caused an increase in glycine the lumbosacral cord. Injection of propiverine into the RPRF or intravenously did not influence lumbosacral cord amino acid levels. Conclusions: These results suggest that the RPRF has an important role in the inhibition of bladder contraction and carbachol or Flavoxate can activate descending RPRF neurons and inhibit bladder contraction via spinal glycinergic neurons.

Hideyuki Yamamoto - One of the best experts on this subject based on the ideXlab platform.