The Experts below are selected from a list of 306 Experts worldwide ranked by ideXlab platform

Anthony D. Stead - One of the best experts on this subject based on the ideXlab platform.

  • changes in protein ubiquitination and the expression of ubiquitin encoding transcripts in daylily petals during Floral Development and senescence
    Physiologia Plantarum, 1994
    Co-Authors: S E Courtney, C. C. Rider, Anthony D. Stead
    Abstract:

    The occurrence of ubiquitin-protein conjugates and the expression of ubiquitin genes during Floral Development and senescence of Hemerocallis fulva (daylily) is reported. Daylily petal proteins were extracted at various stages of Floral Development and the abundance of ubiquitinated proteins determined by western blotting with affinity purified antibody to ubiquitin. Up to 100 ubiquitin conjugates in the molecular mass range 22–220 kDa were detected within the petals during bud Development through to flower opening. The intensity of several of the ubiquitinated protein bands changed markedly during flower opening and/or senescence. Ubiquitinated proteins of apparent molecular masses 160, 105 and 29 kDa were intense in the daylily bud, but rapidly disappeared after flower opening, whereas ubiquitinated proteins of apparent molecular masses 26 and 15.5 kDa increased in intensity as the flowers senesced. Treatment of daylily buds with a 1-h pulse of cycloheximide as the flowers commenced opening prevented these changes; this treatment also delayed the onset of visual signs of senescence by 5–6 days. Analysis by northern blotting demonstrated differential expression of ubiquitin transcripts by the multi-ubiquitin gene family during Floral Development and senescence. Inhibition of petal senescence by treatment with cycloheximide resulted in the accumulation of several ubiquitin-encoding transcripts and the down-regulation of a 3.4 kb transcript. Overall, these studies suggest that ubiquitin is involved in the degradation of many petal proteins during Floral Development and senescence and that, during senescence, this selective degradation is occurring against a background of net proteolysis.

  • Changes in protein ubiquitination and the expression of ubiquitin‐encoding transcripts in daylily petals during Floral Development and senescence
    Physiologia Plantarum, 1994
    Co-Authors: S E Courtney, C. C. Rider, Anthony D. Stead
    Abstract:

    The occurrence of ubiquitin-protein conjugates and the expression of ubiquitin genes during Floral Development and senescence of Hemerocallis fulva (daylily) is reported. Daylily petal proteins were extracted at various stages of Floral Development and the abundance of ubiquitinated proteins determined by western blotting with affinity purified antibody to ubiquitin. Up to 100 ubiquitin conjugates in the molecular mass range 22–220 kDa were detected within the petals during bud Development through to flower opening. The intensity of several of the ubiquitinated protein bands changed markedly during flower opening and/or senescence. Ubiquitinated proteins of apparent molecular masses 160, 105 and 29 kDa were intense in the daylily bud, but rapidly disappeared after flower opening, whereas ubiquitinated proteins of apparent molecular masses 26 and 15.5 kDa increased in intensity as the flowers senesced. Treatment of daylily buds with a 1-h pulse of cycloheximide as the flowers commenced opening prevented these changes; this treatment also delayed the onset of visual signs of senescence by 5–6 days. Analysis by northern blotting demonstrated differential expression of ubiquitin transcripts by the multi-ubiquitin gene family during Floral Development and senescence. Inhibition of petal senescence by treatment with cycloheximide resulted in the accumulation of several ubiquitin-encoding transcripts and the down-regulation of a 3.4 kb transcript. Overall, these studies suggest that ubiquitin is involved in the degradation of many petal proteins during Floral Development and senescence and that, during senescence, this selective degradation is occurring against a background of net proteolysis.

S E Courtney - One of the best experts on this subject based on the ideXlab platform.

  • changes in protein ubiquitination and the expression of ubiquitin encoding transcripts in daylily petals during Floral Development and senescence
    Physiologia Plantarum, 1994
    Co-Authors: S E Courtney, C. C. Rider, Anthony D. Stead
    Abstract:

    The occurrence of ubiquitin-protein conjugates and the expression of ubiquitin genes during Floral Development and senescence of Hemerocallis fulva (daylily) is reported. Daylily petal proteins were extracted at various stages of Floral Development and the abundance of ubiquitinated proteins determined by western blotting with affinity purified antibody to ubiquitin. Up to 100 ubiquitin conjugates in the molecular mass range 22–220 kDa were detected within the petals during bud Development through to flower opening. The intensity of several of the ubiquitinated protein bands changed markedly during flower opening and/or senescence. Ubiquitinated proteins of apparent molecular masses 160, 105 and 29 kDa were intense in the daylily bud, but rapidly disappeared after flower opening, whereas ubiquitinated proteins of apparent molecular masses 26 and 15.5 kDa increased in intensity as the flowers senesced. Treatment of daylily buds with a 1-h pulse of cycloheximide as the flowers commenced opening prevented these changes; this treatment also delayed the onset of visual signs of senescence by 5–6 days. Analysis by northern blotting demonstrated differential expression of ubiquitin transcripts by the multi-ubiquitin gene family during Floral Development and senescence. Inhibition of petal senescence by treatment with cycloheximide resulted in the accumulation of several ubiquitin-encoding transcripts and the down-regulation of a 3.4 kb transcript. Overall, these studies suggest that ubiquitin is involved in the degradation of many petal proteins during Floral Development and senescence and that, during senescence, this selective degradation is occurring against a background of net proteolysis.

  • Changes in protein ubiquitination and the expression of ubiquitin‐encoding transcripts in daylily petals during Floral Development and senescence
    Physiologia Plantarum, 1994
    Co-Authors: S E Courtney, C. C. Rider, Anthony D. Stead
    Abstract:

    The occurrence of ubiquitin-protein conjugates and the expression of ubiquitin genes during Floral Development and senescence of Hemerocallis fulva (daylily) is reported. Daylily petal proteins were extracted at various stages of Floral Development and the abundance of ubiquitinated proteins determined by western blotting with affinity purified antibody to ubiquitin. Up to 100 ubiquitin conjugates in the molecular mass range 22–220 kDa were detected within the petals during bud Development through to flower opening. The intensity of several of the ubiquitinated protein bands changed markedly during flower opening and/or senescence. Ubiquitinated proteins of apparent molecular masses 160, 105 and 29 kDa were intense in the daylily bud, but rapidly disappeared after flower opening, whereas ubiquitinated proteins of apparent molecular masses 26 and 15.5 kDa increased in intensity as the flowers senesced. Treatment of daylily buds with a 1-h pulse of cycloheximide as the flowers commenced opening prevented these changes; this treatment also delayed the onset of visual signs of senescence by 5–6 days. Analysis by northern blotting demonstrated differential expression of ubiquitin transcripts by the multi-ubiquitin gene family during Floral Development and senescence. Inhibition of petal senescence by treatment with cycloheximide resulted in the accumulation of several ubiquitin-encoding transcripts and the down-regulation of a 3.4 kb transcript. Overall, these studies suggest that ubiquitin is involved in the degradation of many petal proteins during Floral Development and senescence and that, during senescence, this selective degradation is occurring against a background of net proteolysis.

C. C. Rider - One of the best experts on this subject based on the ideXlab platform.

  • changes in protein ubiquitination and the expression of ubiquitin encoding transcripts in daylily petals during Floral Development and senescence
    Physiologia Plantarum, 1994
    Co-Authors: S E Courtney, C. C. Rider, Anthony D. Stead
    Abstract:

    The occurrence of ubiquitin-protein conjugates and the expression of ubiquitin genes during Floral Development and senescence of Hemerocallis fulva (daylily) is reported. Daylily petal proteins were extracted at various stages of Floral Development and the abundance of ubiquitinated proteins determined by western blotting with affinity purified antibody to ubiquitin. Up to 100 ubiquitin conjugates in the molecular mass range 22–220 kDa were detected within the petals during bud Development through to flower opening. The intensity of several of the ubiquitinated protein bands changed markedly during flower opening and/or senescence. Ubiquitinated proteins of apparent molecular masses 160, 105 and 29 kDa were intense in the daylily bud, but rapidly disappeared after flower opening, whereas ubiquitinated proteins of apparent molecular masses 26 and 15.5 kDa increased in intensity as the flowers senesced. Treatment of daylily buds with a 1-h pulse of cycloheximide as the flowers commenced opening prevented these changes; this treatment also delayed the onset of visual signs of senescence by 5–6 days. Analysis by northern blotting demonstrated differential expression of ubiquitin transcripts by the multi-ubiquitin gene family during Floral Development and senescence. Inhibition of petal senescence by treatment with cycloheximide resulted in the accumulation of several ubiquitin-encoding transcripts and the down-regulation of a 3.4 kb transcript. Overall, these studies suggest that ubiquitin is involved in the degradation of many petal proteins during Floral Development and senescence and that, during senescence, this selective degradation is occurring against a background of net proteolysis.

  • Changes in protein ubiquitination and the expression of ubiquitin‐encoding transcripts in daylily petals during Floral Development and senescence
    Physiologia Plantarum, 1994
    Co-Authors: S E Courtney, C. C. Rider, Anthony D. Stead
    Abstract:

    The occurrence of ubiquitin-protein conjugates and the expression of ubiquitin genes during Floral Development and senescence of Hemerocallis fulva (daylily) is reported. Daylily petal proteins were extracted at various stages of Floral Development and the abundance of ubiquitinated proteins determined by western blotting with affinity purified antibody to ubiquitin. Up to 100 ubiquitin conjugates in the molecular mass range 22–220 kDa were detected within the petals during bud Development through to flower opening. The intensity of several of the ubiquitinated protein bands changed markedly during flower opening and/or senescence. Ubiquitinated proteins of apparent molecular masses 160, 105 and 29 kDa were intense in the daylily bud, but rapidly disappeared after flower opening, whereas ubiquitinated proteins of apparent molecular masses 26 and 15.5 kDa increased in intensity as the flowers senesced. Treatment of daylily buds with a 1-h pulse of cycloheximide as the flowers commenced opening prevented these changes; this treatment also delayed the onset of visual signs of senescence by 5–6 days. Analysis by northern blotting demonstrated differential expression of ubiquitin transcripts by the multi-ubiquitin gene family during Floral Development and senescence. Inhibition of petal senescence by treatment with cycloheximide resulted in the accumulation of several ubiquitin-encoding transcripts and the down-regulation of a 3.4 kb transcript. Overall, these studies suggest that ubiquitin is involved in the degradation of many petal proteins during Floral Development and senescence and that, during senescence, this selective degradation is occurring against a background of net proteolysis.

Maria Von Korff - One of the best experts on this subject based on the ideXlab platform.

  • flowering locus t3 controls spikelet initiation but not Floral Development
    Plant Physiology, 2018
    Co-Authors: Muhammad Aman Mulki, Xiaojing Bi, Maria Von Korff
    Abstract:

    In many angiosperm plants, FLOWERING LOCUS T (FT)-like genes have duplicated and functionally diverged to control different reproductive traits or stages. Barley (Hordeum vulgare) carries several FT-like genes, the functions of which are not well understood. We characterized the role of HvFT3 in the vegetative and reproductive Development of barley. Overexpression of HvFT3 accelerated the initiation of spikelet primordia and the early reproductive Development of spring barley independently of the photoperiod. However, HvFT3 overexpression did not accelerate Floral Development, and inflorescences aborted under short days, suggesting that HvFT3 controls spikelet initiation but not Floral Development. Analysis of a nonfunctional HvFT3 allele supported the specific effects of this gene on spikelet initiation independent of the photoperiod. HvFT3 caused the up-regulation of the winter and spring alleles of the vernalization gene VERNALIZATION1 (VRN-H1) in nonvernalized plants and was therefore dominant over the repressive effects of the vernalization pathway. Global transcriptome analysis in developing main shoot apices of the transgenic lines showed that HvFT3 modified the expression of genes involved in hormone synthesis and response, of Floral homeotic genes, and of barley row-type genes SIX-ROWED-SPIKE1 (VRS1), SIX-ROWED-SPIKE4 (VRS4), and INTERMEDIUM C. Understanding the specific functions of individual FT-like genes will allow modification of individual phases of preanthesis Development and thereby adaptation to different environments and improved yield.

  • flowering locus t3 controls spikelet initiation but not Floral Development
    Plant Physiology, 2018
    Co-Authors: Muhammad Aman Mulki, Xiaojing Bi, Maria Von Korff
    Abstract:

    In many angiosperm plants, FLOWERING LOCUS T (FT)-like genes have duplicated and functionally diverged to control different reproductive traits or stages. Barley (Hordeum vulgare) carries several FT-like genes, the functions of which are not well understood. We characterized the role of HvFT3 in the vegetative and reproductive Development of barley. Overexpression of HvFT3 accelerated the initiation of spikelet primordia and the early reproductive Development of spring barley independently of the photoperiod. However, HvFT3 overexpression did not accelerate Floral Development, and inflorescences aborted under short days, suggesting that HvFT3 controls spikelet initiation but not Floral Development. Analysis of a nonfunctional HvFT3 allele supported the specific effects of this gene on spikelet initiation independent of the photoperiod. HvFT3 caused the up-regulation of the winter and spring alleles of the vernalization gene VERNALIZATION1 (VRN-H1) in nonvernalized plants and was therefore dominant over the repressive effects of the vernalization pathway. Global transcriptome analysis in developing main shoot apices of the transgenic lines showed that HvFT3 modified the expression of genes involved in hormone synthesis and response, of Floral homeotic genes, and of barley row-type genes SIX-ROWED-SPIKE1 (VRS1), SIX-ROWED-SPIKE4 (VRS4), and INTERMEDIUM C. Understanding the specific functions of individual FT-like genes will allow modification of individual phases of preanthesis Development and thereby adaptation to different environments and improved yield.

Joseph R Ecker - One of the best experts on this subject based on the ideXlab platform.

  • della proteins and gibberellin regulated seed germination and Floral Development in arabidopsis
    Plant Physiology, 2004
    Co-Authors: Ludmila Tyler, Alyssa Dill, Jianhong Hu, Jose M Alonso, S. Thomas, Joseph R Ecker
    Abstract:

    RGA (repressor of ga1-3) and GAI (gibberellin insensitive) are negative regulators of plant hormone gibberellin (GA) signaling in Arabidopsis. The GA-deficient mutant ga1-3 is a nongerminating, extreme dwarf that flowers late and produces male-sterile flowers. The rga and gai null alleles interact synergistically to rescue vegetative growth and Floral initiation in ga1-3, indicating that RGA and GAI are major repressors for these processes. However, rga and gai in combination cannot rescue seed germination or Floral Development in ga1-3. RGA and GAI belong to the DELLA subfamily within the GRAS family of plant regulatory proteins. Three additional DELLA proteins RGL1, RGL2, and RGL3 are present in Arabidopsis. Previous studies provided evidence that RGL2 and possibly RGL1 control seed germination. To investigate further the function of the RGL genes, we examined the expression profiles of all 5 DELLA protein genes by real-time PCR. RGA and, to a lesser extent, GAI mRNAs were expressed ubiquitously in all tissues, whereas RGL1, 2, and 3 transcripts were present at high levels only in germinating seeds and/or flowers and siliques. Using the newly isolated rgl1, rgl2, and rgl3 T-DNA insertion mutants, we demonstrated that RGL2 is the major repressor in seed germination. We further provided evidence that RGA, RGL1, and RGL2 are all involved in modulating Floral Development. Interestingly, RGL2 expression is regulated not only at the transcript level. We showed that RGL2 protein in imbibed seeds is rapidly degraded by GA treatment and that the F-box protein SLY1 is required for RGL2 degradation to occur.