The Experts below are selected from a list of 270 Experts worldwide ranked by ideXlab platform
Xiaojun Peng - One of the best experts on this subject based on the ideXlab platform.
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a dual targeted theranostic photosensitizer based on a tadf Fluorescein Derivative
Journal of Controlled Release, 2019Co-Authors: Zhiwei Liu, Wenlong Chen, Fengling Song, Gaobo Hong, Xiaojun Peng, Wenbo Shi, Bo Song, Xiaoqing XiongAbstract:Specific diagnosis and therapy of cancer is still a challenge in biomedical research. Photodynamic therapy (PDT) has emerged as a novel therapeutic modality for cancer treatment. However, the traditional PDT photosensitizers often exhibit low specific selectivity. In this study, we have reported a dual-targeted theranostic photosensitizer FL-RGD by covalently conjugating tumor marker cyclic arginine-glycine-aspartic acid tripeptide (RGD) and a Fluorescein Derivative FL which has a property of thermally activated delayed fluorescence (TADF) and a long triplet lifetime for efficient PDT. The FL-RGD can target tumor tissues and further locate lysosomes of tumor cells to concurrently achieve the cancers' specific diagnosis and efficient treatment. The mechanism of its highly efficient PDT was attributed to the damage of lysosome via 1O2. Besides, FL-RGD has the potential to be utilized in depth imaging and treatment by two-photon excitation. The actual diagnosis performance of FL-RGD was proved by fluorescence imaging of living cells and tumor bearing mice. The therapy performance was proved by MTT assays, fluorescence-activated cell sorting (FACS) analysis and PDT experiments on tumor bearing mice. The research obviously exhibited the potential of FL-RGD for tumor theranostics in vivo and in vitro.
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red to blue photon up conversion with high efficiency based on a tadf Fluorescein Derivative
Chemical Communications, 2019Co-Authors: Wenlong Chen, Fengling Song, Shanliang Tang, Gaobo Hong, Xiaojun PengAbstract:A new photon up-conversion system with a TADF Fluorescein Derivative as a photosensitizer was developed to achieve a quite large anti-Stokes shift from red to blue with a fairly high up-conversion emission quantum yield. This TADF photosensitizer has a very small ΔEST to provide a 207 nm anti-Stokes shift photon up-conversion. Meanwhile, it has a quite long triplet state lifetime (22.11 μs) and a large molar extinction coefficient to assure a high up-conversion efficiency of 11.2%. These results fully demonstrated the potential of the TADF Fluorescein Derivative as the photosensitizer used in photon up-conversion for the first time.
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nitroreductase activatable theranostic molecules with high pdt efficiency under mild hypoxia based on a tadf Fluorescein Derivative
ACS Applied Materials & Interfaces, 2019Co-Authors: Zhiwei Liu, Fengling Song, Wenbo Shi, Bo Song, Gagik G Gurzadyan, Huiyi Yin, Ri Liang, Xiaojun PengAbstract:High specificity detection and site-specific therapy are still the main challenges for theranostic anticancer prodrugs. In this work, we reported two smart activatable theranostic molecules based on a thermally activated delayed fluorescence Fluorescein Derivative. Nitroreductase induced by a mild hypoxia microenvironment of a solid tumor was used to activate the fluorescence and photodynamic therapy (PDT) efficiency by employing the intramolecular photoinduced electron transfer mechanism. A high PDT efficiency under 10% oxygen concentration was achieved, which is better than that of porphyrin (PpIX), a traditional photosensitizer. Such an excellent PDT efficiency can be attributed to lysosome disruption because the theranostic molecule can specifically enter the lysosomes of cells. Importantly, the strategy of targeting the mild hypoxic cells in the edge of tumor tissue could heal the “Achilles’ heel” of traditional PDT. We believe that this theranostic molecule has a high potential to be applied in clin...
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nitroreductase activatable theranostic molecules with high pdt efficiency under mild hypoxia based on a tadf Fluorescein Derivative
ACS Applied Materials & Interfaces, 2019Co-Authors: Zhiwei Liu, Fengling Song, Wenbo Shi, Bo Song, Gagik G Gurzadyan, Huiyi Yin, Ri Liang, Xiaojun PengAbstract:High specificity detection and site-specific therapy are still the main challenges for theranostic anticancer prodrugs. In this work, we reported two smart activatable theranostic molecules based o...
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Nitroreductase-Activatable Theranostic Molecules with High PDT Efficiency under Mild Hypoxia Based on a TADF Fluorescein Derivative
2019Co-Authors: Zhiwei Liu, Fengling Song, Wenbo Shi, Bo Song, Gagik G Gurzadyan, Huiyi Yin, Ri Liang, Xiaojun PengAbstract:High specificity detection and site-specific therapy are still the main challenges for theranostic anticancer prodrugs. In this work, we reported two smart activatable theranostic molecules based on a thermally activated delayed fluorescence Fluorescein Derivative. Nitroreductase induced by a mild hypoxia microenvironment of a solid tumor was used to activate the fluorescence and photodynamic therapy (PDT) efficiency by employing the intramolecular photoinduced electron transfer mechanism. A high PDT efficiency under 10% oxygen concentration was achieved, which is better than that of porphyrin (PpIX), a traditional photosensitizer. Such an excellent PDT efficiency can be attributed to lysosome disruption because the theranostic molecule can specifically enter the lysosomes of cells. Importantly, the strategy of targeting the mild hypoxic cells in the edge of tumor tissue could heal the “Achilles’ heel” of traditional PDT. We believe that this theranostic molecule has a high potential to be applied in clinical investigation as a theranostic anticancer prodrug
Fengling Song - One of the best experts on this subject based on the ideXlab platform.
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a dual targeted theranostic photosensitizer based on a tadf Fluorescein Derivative
Journal of Controlled Release, 2019Co-Authors: Zhiwei Liu, Wenlong Chen, Fengling Song, Gaobo Hong, Xiaojun Peng, Wenbo Shi, Bo Song, Xiaoqing XiongAbstract:Specific diagnosis and therapy of cancer is still a challenge in biomedical research. Photodynamic therapy (PDT) has emerged as a novel therapeutic modality for cancer treatment. However, the traditional PDT photosensitizers often exhibit low specific selectivity. In this study, we have reported a dual-targeted theranostic photosensitizer FL-RGD by covalently conjugating tumor marker cyclic arginine-glycine-aspartic acid tripeptide (RGD) and a Fluorescein Derivative FL which has a property of thermally activated delayed fluorescence (TADF) and a long triplet lifetime for efficient PDT. The FL-RGD can target tumor tissues and further locate lysosomes of tumor cells to concurrently achieve the cancers' specific diagnosis and efficient treatment. The mechanism of its highly efficient PDT was attributed to the damage of lysosome via 1O2. Besides, FL-RGD has the potential to be utilized in depth imaging and treatment by two-photon excitation. The actual diagnosis performance of FL-RGD was proved by fluorescence imaging of living cells and tumor bearing mice. The therapy performance was proved by MTT assays, fluorescence-activated cell sorting (FACS) analysis and PDT experiments on tumor bearing mice. The research obviously exhibited the potential of FL-RGD for tumor theranostics in vivo and in vitro.
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red to blue photon up conversion with high efficiency based on a tadf Fluorescein Derivative
Chemical Communications, 2019Co-Authors: Wenlong Chen, Fengling Song, Shanliang Tang, Gaobo Hong, Xiaojun PengAbstract:A new photon up-conversion system with a TADF Fluorescein Derivative as a photosensitizer was developed to achieve a quite large anti-Stokes shift from red to blue with a fairly high up-conversion emission quantum yield. This TADF photosensitizer has a very small ΔEST to provide a 207 nm anti-Stokes shift photon up-conversion. Meanwhile, it has a quite long triplet state lifetime (22.11 μs) and a large molar extinction coefficient to assure a high up-conversion efficiency of 11.2%. These results fully demonstrated the potential of the TADF Fluorescein Derivative as the photosensitizer used in photon up-conversion for the first time.
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nitroreductase activatable theranostic molecules with high pdt efficiency under mild hypoxia based on a tadf Fluorescein Derivative
ACS Applied Materials & Interfaces, 2019Co-Authors: Zhiwei Liu, Fengling Song, Wenbo Shi, Bo Song, Gagik G Gurzadyan, Huiyi Yin, Ri Liang, Xiaojun PengAbstract:High specificity detection and site-specific therapy are still the main challenges for theranostic anticancer prodrugs. In this work, we reported two smart activatable theranostic molecules based on a thermally activated delayed fluorescence Fluorescein Derivative. Nitroreductase induced by a mild hypoxia microenvironment of a solid tumor was used to activate the fluorescence and photodynamic therapy (PDT) efficiency by employing the intramolecular photoinduced electron transfer mechanism. A high PDT efficiency under 10% oxygen concentration was achieved, which is better than that of porphyrin (PpIX), a traditional photosensitizer. Such an excellent PDT efficiency can be attributed to lysosome disruption because the theranostic molecule can specifically enter the lysosomes of cells. Importantly, the strategy of targeting the mild hypoxic cells in the edge of tumor tissue could heal the “Achilles’ heel” of traditional PDT. We believe that this theranostic molecule has a high potential to be applied in clin...
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nitroreductase activatable theranostic molecules with high pdt efficiency under mild hypoxia based on a tadf Fluorescein Derivative
ACS Applied Materials & Interfaces, 2019Co-Authors: Zhiwei Liu, Fengling Song, Wenbo Shi, Bo Song, Gagik G Gurzadyan, Huiyi Yin, Ri Liang, Xiaojun PengAbstract:High specificity detection and site-specific therapy are still the main challenges for theranostic anticancer prodrugs. In this work, we reported two smart activatable theranostic molecules based o...
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Nitroreductase-Activatable Theranostic Molecules with High PDT Efficiency under Mild Hypoxia Based on a TADF Fluorescein Derivative
2019Co-Authors: Zhiwei Liu, Fengling Song, Wenbo Shi, Bo Song, Gagik G Gurzadyan, Huiyi Yin, Ri Liang, Xiaojun PengAbstract:High specificity detection and site-specific therapy are still the main challenges for theranostic anticancer prodrugs. In this work, we reported two smart activatable theranostic molecules based on a thermally activated delayed fluorescence Fluorescein Derivative. Nitroreductase induced by a mild hypoxia microenvironment of a solid tumor was used to activate the fluorescence and photodynamic therapy (PDT) efficiency by employing the intramolecular photoinduced electron transfer mechanism. A high PDT efficiency under 10% oxygen concentration was achieved, which is better than that of porphyrin (PpIX), a traditional photosensitizer. Such an excellent PDT efficiency can be attributed to lysosome disruption because the theranostic molecule can specifically enter the lysosomes of cells. Importantly, the strategy of targeting the mild hypoxic cells in the edge of tumor tissue could heal the “Achilles’ heel” of traditional PDT. We believe that this theranostic molecule has a high potential to be applied in clinical investigation as a theranostic anticancer prodrug
Hiroshi Terada - One of the best experts on this subject based on the ideXlab platform.
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binding of the Fluorescein Derivative eosin y to the mitochondrial adp atp carrier characterization of the adenine nucleotide binding site
Biochemistry, 1998Co-Authors: Eiji Majima, Nana Yamaguchi, Hiroshi Chuman, Yasuo Shinohara, Mayumi Ishida, Satoru Goto, Hiroshi TeradaAbstract:As the SH-reactive Fluorescein Derivative eosin-5-maleimide (EMA) specifically labels Cys159 in the second loop facing the matrix space (loop M2) of the ADP/ATP carrier in bovine heart submitochondrial particles [Majima, E., Koike, H., Hong, Y.-M., Shinohara, Y., and Terada, H. (1993) J. Biol. Chem. 268, 22181−22187], we studied the interaction of non-SH-reactive eosin Y, an analog of EMA, with the carrier under various conditions to characterize its binding. Eosin Y was found to inhibit ADP transport by binding to loop M2 in submitochondrial particles, but not in mitochondria. Its Ki for transport (0.33 μM) was found to be very similar to its Kd (0.53 μM) for specific binding to the carrier. Bound eosin Y was displaced by the transport substrates ADP and ATP, but not by untransportable GTP, suggesting that eosin Y bound to the specific binding site of ADP and ATP. The three-dimensional structure and electrostatic features of eosin Y were very similar to those of ADP, and the hydrophobic property and diva...
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binding of the Fluorescein Derivative eosin y to the mitochondrial adp atp carrier characterization of the adenine nucleotide binding site
Biochemistry, 1998Co-Authors: Eiji Majima, Nana Yamaguchi, Hiroshi Chuman, Yasuo Shinohara, Mayumi Ishida, Satoru Goto, Hiroshi TeradaAbstract:As the SH-reactive Fluorescein Derivative eosin-5-maleimide (EMA) specifically labels Cys159 in the second loop facing the matrix space (loop M2) of the ADP/ATP carrier in bovine heart submitochondrial particles [Majima, E., Koike, H., Hong, Y.-M., Shinohara, Y., and Terada, H. (1993) J. Biol. Chem. 268, 22181-22187], we studied the interaction of non-SH-reactive eosin Y, an analog of EMA, with the carrier under various conditions to characterize its binding. Eosin Y was found to inhibit ADP transport by binding to loop M2 in submitochondrial particles, but not in mitochondria. Its Ki for transport (0.33 microM) was found to be very similar to its Kd (0.53 microM) for specific binding to the carrier. Bound eosin Y was displaced by the transport substrates ADP and ATP, but not by untransportable GTP, suggesting that eosin Y bound to the specific binding site of ADP and ATP. The three-dimensional structure and electrostatic features of eosin Y were very similar to those of ADP, and the hydrophobic property and divalent charge of eosin Y were very important for its binding to the carrier. Based on these results, the features of the binding site of the transport substrates are considered.
Zhiwei Liu - One of the best experts on this subject based on the ideXlab platform.
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a dual targeted theranostic photosensitizer based on a tadf Fluorescein Derivative
Journal of Controlled Release, 2019Co-Authors: Zhiwei Liu, Wenlong Chen, Fengling Song, Gaobo Hong, Xiaojun Peng, Wenbo Shi, Bo Song, Xiaoqing XiongAbstract:Specific diagnosis and therapy of cancer is still a challenge in biomedical research. Photodynamic therapy (PDT) has emerged as a novel therapeutic modality for cancer treatment. However, the traditional PDT photosensitizers often exhibit low specific selectivity. In this study, we have reported a dual-targeted theranostic photosensitizer FL-RGD by covalently conjugating tumor marker cyclic arginine-glycine-aspartic acid tripeptide (RGD) and a Fluorescein Derivative FL which has a property of thermally activated delayed fluorescence (TADF) and a long triplet lifetime for efficient PDT. The FL-RGD can target tumor tissues and further locate lysosomes of tumor cells to concurrently achieve the cancers' specific diagnosis and efficient treatment. The mechanism of its highly efficient PDT was attributed to the damage of lysosome via 1O2. Besides, FL-RGD has the potential to be utilized in depth imaging and treatment by two-photon excitation. The actual diagnosis performance of FL-RGD was proved by fluorescence imaging of living cells and tumor bearing mice. The therapy performance was proved by MTT assays, fluorescence-activated cell sorting (FACS) analysis and PDT experiments on tumor bearing mice. The research obviously exhibited the potential of FL-RGD for tumor theranostics in vivo and in vitro.
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nitroreductase activatable theranostic molecules with high pdt efficiency under mild hypoxia based on a tadf Fluorescein Derivative
ACS Applied Materials & Interfaces, 2019Co-Authors: Zhiwei Liu, Fengling Song, Wenbo Shi, Bo Song, Gagik G Gurzadyan, Huiyi Yin, Ri Liang, Xiaojun PengAbstract:High specificity detection and site-specific therapy are still the main challenges for theranostic anticancer prodrugs. In this work, we reported two smart activatable theranostic molecules based on a thermally activated delayed fluorescence Fluorescein Derivative. Nitroreductase induced by a mild hypoxia microenvironment of a solid tumor was used to activate the fluorescence and photodynamic therapy (PDT) efficiency by employing the intramolecular photoinduced electron transfer mechanism. A high PDT efficiency under 10% oxygen concentration was achieved, which is better than that of porphyrin (PpIX), a traditional photosensitizer. Such an excellent PDT efficiency can be attributed to lysosome disruption because the theranostic molecule can specifically enter the lysosomes of cells. Importantly, the strategy of targeting the mild hypoxic cells in the edge of tumor tissue could heal the “Achilles’ heel” of traditional PDT. We believe that this theranostic molecule has a high potential to be applied in clin...
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nitroreductase activatable theranostic molecules with high pdt efficiency under mild hypoxia based on a tadf Fluorescein Derivative
ACS Applied Materials & Interfaces, 2019Co-Authors: Zhiwei Liu, Fengling Song, Wenbo Shi, Bo Song, Gagik G Gurzadyan, Huiyi Yin, Ri Liang, Xiaojun PengAbstract:High specificity detection and site-specific therapy are still the main challenges for theranostic anticancer prodrugs. In this work, we reported two smart activatable theranostic molecules based o...
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Nitroreductase-Activatable Theranostic Molecules with High PDT Efficiency under Mild Hypoxia Based on a TADF Fluorescein Derivative
2019Co-Authors: Zhiwei Liu, Fengling Song, Wenbo Shi, Bo Song, Gagik G Gurzadyan, Huiyi Yin, Ri Liang, Xiaojun PengAbstract:High specificity detection and site-specific therapy are still the main challenges for theranostic anticancer prodrugs. In this work, we reported two smart activatable theranostic molecules based on a thermally activated delayed fluorescence Fluorescein Derivative. Nitroreductase induced by a mild hypoxia microenvironment of a solid tumor was used to activate the fluorescence and photodynamic therapy (PDT) efficiency by employing the intramolecular photoinduced electron transfer mechanism. A high PDT efficiency under 10% oxygen concentration was achieved, which is better than that of porphyrin (PpIX), a traditional photosensitizer. Such an excellent PDT efficiency can be attributed to lysosome disruption because the theranostic molecule can specifically enter the lysosomes of cells. Importantly, the strategy of targeting the mild hypoxic cells in the edge of tumor tissue could heal the “Achilles’ heel” of traditional PDT. We believe that this theranostic molecule has a high potential to be applied in clinical investigation as a theranostic anticancer prodrug
Hong Qun Luo - One of the best experts on this subject based on the ideXlab platform.
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multidimensional optical sensing platform for detection of heparin and reversible molecular logic gate operation based on the phloxine b polyethyleneimine system
Analytical Chemistry, 2015Co-Authors: Yu Ling, Zhong Feng Gao, Qian Zhou, Hong Qun LuoAbstract:A multidimensional optical sensing platform which combines the advantages of resonance Rayleigh scattering (RRS), fluorescence, and colorimetry has been designed for detection of heparin. Phloxine B, a Fluorescein Derivative showing the special RRS spectrum in the long wavelength region, was selected to develop an easy-to-get system which can achieve switch-on sensing to obtain high sensitivity. The noise level of RRS in the long wavelength region is much weaker, and the reproducibility is much better; in this way, the sensitivity and selectivity can be improved. In the absence of heparin, the phloxine B and polyethyleneimine (PEI) form a complex through electrostatic interaction. Thus, the RRS signal at 554 nm is low; the phloxine B fluorescence is quenched, and the absorption signal is low. In the presence of heparin, competitive binding occurred between phloxine B and heparin toward PEI; then, phloxine B is gradually released from the phloxine B/PEI complex, causing obvious enhancement of the RRS, fluo...
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Multidimensional Optical Sensing Platform for Detection of Heparin and Reversible Molecular Logic Gate Operation Based on the Phloxine B/Polyethyleneimine System
2015Co-Authors: Yu Ling, Zhong Feng Gao, Qian Zhou, Hong Qun LuoAbstract:A multidimensional optical sensing platform which combines the advantages of resonance Rayleigh scattering (RRS), fluorescence, and colorimetry has been designed for detection of heparin. Phloxine B, a Fluorescein Derivative showing the special RRS spectrum in the long wavelength region, was selected to develop an easy-to-get system which can achieve switch-on sensing to obtain high sensitivity. The noise level of RRS in the long wavelength region is much weaker, and the reproducibility is much better; in this way, the sensitivity and selectivity can be improved. In the absence of heparin, the phloxine B and polyethyleneimine (PEI) form a complex through electrostatic interaction. Thus, the RRS signal at 554 nm is low; the phloxine B fluorescence is quenched, and the absorption signal is low. In the presence of heparin, competitive binding occurred between phloxine B and heparin toward PEI; then, phloxine B is gradually released from the phloxine B/PEI complex, causing obvious enhancement of the RRS, fluorescence, and absorption signals. Besides, the desorption of phloxine B is less effective for the heparin analogues, such as hyaluronic acid and chondroitin sulfate. In addition, the system presents a low detection limit of heparin to 5.0 × 10–4 U mL–1 and can also be applied to the detection of heparin in heparin sodium injection and 50% human serum samples with satisfactory results. Finally, the potential application of this method in reversible on–off molecular logic gate fabrication was discussed using the triple-channel optical signals as outputs