The Experts below are selected from a list of 1752 Experts worldwide ranked by ideXlab platform
Kristina Narfström - One of the best experts on this subject based on the ideXlab platform.
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Fluorescence Histochemistry of ABCA4, cone photoreceptors, and autoFluorescence in the canine retina.
2019Co-Authors: Suvi Mäkeläinen, Marta Gòdia, Minas Hellsand, Agnese Viluma, Daniela Hahn, Karim Makdoumi, Caroline J. Zeiss, Cathryn Mellersh, Sally L. Ricketts, Kristina NarfströmAbstract:(A-C) Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), and DAPI nuclear staining (blue) in wild-type (ABCA4+/+), heterozygous (ABCA4+/-), and affected (ABCA4-/-) retinas. PNA labels cone photoreceptors. AutoFluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. (D) Bar graph with the average number of DAPI-stained nuclei within a given region of the ONL and the INL. (E-G) Fluorescence micrographs of RPE without immunoHistochemistry show autoFluorescence. (H) Bar graph with background-corrected mean autoFluorescence-intensity in the RPE. Note the reduction of ABCA4-immunoreactivity and PNA binding, higher autoFluorescence, and fewer nuclei in the ONL in the ABCA4-/- compared to ABCA4+/+ or ABCA4+/- retinas. All scale bars = 50 μm; RPE = retinal pigment epithelium; ONL = outer nuclear layer; INL = inner nuclear layer; Because there was only one individual per genotype, the statistics are valid for the technical replicates. ANOVA with Tukey’s post hoc test, n = 6; **P < 0.01; ***P < 0.001; mean ± S.D.
Suvi Mäkeläinen - One of the best experts on this subject based on the ideXlab platform.
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Fluorescence Histochemistry of ABCA4, cone photoreceptors, and autoFluorescence in the canine retina.
2019Co-Authors: Suvi Mäkeläinen, Marta Gòdia, Minas Hellsand, Agnese Viluma, Daniela Hahn, Karim Makdoumi, Caroline J. Zeiss, Cathryn Mellersh, Sally L. Ricketts, Kristina NarfströmAbstract:(A-C) Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), and DAPI nuclear staining (blue) in wild-type (ABCA4+/+), heterozygous (ABCA4+/-), and affected (ABCA4-/-) retinas. PNA labels cone photoreceptors. AutoFluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. (D) Bar graph with the average number of DAPI-stained nuclei within a given region of the ONL and the INL. (E-G) Fluorescence micrographs of RPE without immunoHistochemistry show autoFluorescence. (H) Bar graph with background-corrected mean autoFluorescence-intensity in the RPE. Note the reduction of ABCA4-immunoreactivity and PNA binding, higher autoFluorescence, and fewer nuclei in the ONL in the ABCA4-/- compared to ABCA4+/+ or ABCA4+/- retinas. All scale bars = 50 μm; RPE = retinal pigment epithelium; ONL = outer nuclear layer; INL = inner nuclear layer; Because there was only one individual per genotype, the statistics are valid for the technical replicates. ANOVA with Tukey’s post hoc test, n = 6; **P < 0.01; ***P < 0.001; mean ± S.D.
Goldstein M - One of the best experts on this subject based on the ideXlab platform.
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Expression of tyrosine hydroxylase-like immunoreactivity in cell bodies in intraocular spinal cord grafts: a comparison with classical Falck-Hillarp Histochemistry.
Journal of chemical neuroanatomy, 1990Co-Authors: Henschen A, Goldstein MAbstract:Earlier classical monoamine Fluorescence histochemical and immunohistochemical studies describing the distribution of catecholamine neurons in the central nervous system led to the conclusion that all catecholamine fibre systems in the spinal cord have a supra-spinal origin. However, recent reports describing catecholamine neurons in upper cervical segments and at the lumbosacral junction have modified this picture. Isolated intraocular grafts of spinal cord offer a possible model with which to evaluate the presence of intrinsic monoamine neurons. Using immunoHistochemistry with antiserum to tyrosine hydroxylase (TH) and Falck-Hillarp Fluorescence Histochemistry, such grafts from lower cervical segments were found to contain TH-immunoreactive perikarya. However, it was not possible to visualize cell bodies in grafts processed for Fluorescence Histochemistry. These findings raise questions about the sensitivity of the monoamine Fluorescence histochemical method, and about the specificity of TH-immunoHistochemistry. Perhaps TH-positive, catecholamine-negative neurons are more common than previously held or alternatively, TH expression in non-catecholamine neurons is induced by perturbations such as grafting brain tissue.
Liu Xianfeng - One of the best experts on this subject based on the ideXlab platform.
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Role of Bone Marrow Stem Cells in the Regeneration of Renal Tubular Epithelial Cells in Mice
Chinese Journal of Integrated Traditional and Western Nephrology, 2007Co-Authors: Liu XianfengAbstract:Objective:To demonstrate bone marrow stem cells can differentiate into renal tubular epithelial cells by an mice bone marrow transplantation model.Methods:Transgenic C57B/6 mice labeled with green flouresent protein(GFP)as bone marrow donors,C57B/6 mice without Fluorescence label received of bone marrow transplantation and werer sacrificed on day 56 and day 84 after transplantation so as to observe the differentiation of bone marrow stem cells in the kidneys observed by Fluorescence Histochemistry and immunoHistochemistry.Results:Green Fluorescence was seen in renal tubular epithelium on day 56 and 84 after bone marrow transplantation,further prove of these cells location in tubular epithelium was obtained by laser scanning confocal microscope,Fluorescence Histochemistry show these cells expressed renal tubular epithelium specific protein megalin.Conclusion:Bone marrow stem cells can participate in regeneration of renal tubular epithelium in the physical condition.
S J Wiegand - One of the best experts on this subject based on the ideXlab platform.
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Fluorescence histochemical techniques for catecholamines as tools in neurobiology.
Journal of microscopy, 1990Co-Authors: D L Felten, S Y Felten, J R Sladek, M D Notter, S L Carlson, D L Bellinger, S J WiegandAbstract:Formaldehyde-induced and glyoxylic-acid-induced Fluorescence Histochemistry permits the tissue localization of catecholamines in the central nervous system (CNS) and peripheral nervous system (PNS), and in culture. Counterstains such as ethidium bromide provide excellent background identification of specific innervated regions in both the CNS and the periphery. Use of Fluorescence Histochemistry with immunocytochemistry can elucidate catecholamine-peptide relationships. Gelatin-ink perfusion used with Fluorescence Histochemistry permits the investigation of neuro-vascular relationships and documentation of vascular and parenchymal compartmentation of innervation. Combined use of Fluorescence Histochemistry and retrograde tracing methods demonstrates the specific cellular sources of innervation of target regions. Micropunch neurochemical analysis provides quantitative data for correlation with Fluorescence Histochemistry within a target region of innervation, and microspectrofluorometric analysis provides a semi-quantitative evaluation of the amount of fluorophore within a target region or within specific subcellular compartments such as the cell body or terminals.