The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Xiangzhi Song - One of the best experts on this subject based on the ideXlab platform.
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a ratiometric merocyanine based Fluorescent Probe for detecting hydrazine in living cells and zebra fish
Chinese Chemical Letters, 2020Co-Authors: Jinliang Han, Benhua Wang, Xiuxiu Yue, Jingpei Wang, Yun Zhang, Xiangzhi SongAbstract:Abstract We developed a merocyanine-based Fluorescent Probe, NEPB, for tracing hydrazine (N2H4) in a ratiometric manner with large Stokes shifts and long emission wavelength. The fluorescence color of Probe NEPB changed from green to yellow upon addition of hydrazine. Probe NEPB displayed high selectivity and sensitivity to hydrazine in solution, and could ratiometrically monitor N2H4 in living cells and zebrafish with low cytotoxicity.
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a real time colorimetric and ratiometric Fluorescent Probe for rapid detection of so2 derivatives in living cells based on a near infrared benzopyrylium dye
RSC Advances, 2015Co-Authors: Wenqiang Chen, Xingjiang Liu, Song Chen, Xiangzhi Song, Jian KangAbstract:A benzopyrylium-based dye, Probe 1, was developed as a near-infrared Fluorescent Probe for the detection of SO2 derivatives (sulfite/bisulfite) in pure aqueous solution. In the presence of sulfite/bisulfite, the solution of Probe 1 displayed significant changes in its absorption (from 650 nm to 420 nm) and emission (from 690 nm to 489 nm) spectra. Probe 1 exhibits excellent selectivity and high sensitivity toward sulfite/bisulfite and the detection limit (S/N = 3) for bisulfite is as low as 10.4 nM. Specifically, Probe 1 shows a fast response time (within 10 s) toward sulfite/bisulfite, which makes it capable for real-time sensing or imaging both in vitro and in vivo assays. Probe 2, an analogue of Probe 1, as a turn-on Fluorescent Probe was also developed and displayed similar properties for the detection of sulfite/bisulfite. The demonstration of Probe 1 as a ratiometric Fluorescent Probe and Probe 2 as a turn-on Fluorescent Probe for the detection of SO2 derivatives was achieved in living HeLa cells and A431 cells, respectively.
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highly selective red emitting h2s Fluorescent Probe with a large stokes shift
Dyes and Pigments, 2015Co-Authors: Wenqiang Chen, Song Chen, Xiangzhi Song, Bingjiang Zhou, Hongbo Wang, Hongyan ZhangAbstract:Abstract The O-2,4-dinitrobenzensulfonate of 1,3-bis(bispyridin-2ylimino)isoindolin-4-ol has been developed as a novel red-emitting Fluorescent Probe for the detection of H2S. The dinitrobenzenesulfonate moiety in the Probe both prohibits the excited state intramolecular proton transfer process and produces a photo-induced electron transfer process, which renders the Probe non-Fluorescent in the absence of the analyte. In the presence of H2S a specific H2S-mediated cleavage reaction converts the Probe into 1,3-bis(bispyridin-2-ylimino)isoindolin-4-ol which exhibits a strong red fluorescence with a large Stokes shift (218 nm) via an excited state intramolecular proton transfer process upon excitation. It's noteworthy that this new Probe shows good selectivity and sensitivity to H2S over glutathione, cysteine and homocysteine. Moreover successful detection and imaging of intracellular H2S in living cells was achieved. To our knowledge this is the first application of this type of Fluorescent Probe for intracellular H2S detection.
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a highly sulfite selective ratiometric Fluorescent Probe based on esipt
RSC Advances, 2012Co-Authors: Song Chen, Peng Hou, Jianxiu Wang, Xiangzhi SongAbstract:The levulinate ester of 2-(benzothiazol-2-yl)phenol, 1, has been developed as a ratiometric Fluorescent Probe for identifying and quantitating sulfite anions. The mechanism of action is based on the sulfite-triggered intramolecular cleavage of the levulinate moiety to give 1 which, when excited with 310 nm light, decays to its ground state via an excited state intramolecular proton transfer (ESIPT) mediated pathway. We show that the intensity of the ESIPT Fluorescent signal relative to that of 1 is proportional to sulfite concentration. This new Probe shows good selectivity and high sensitivity for sulfite over other typically encountered anions (F−, Cl−, Br−, I−, HPO42−, SO42−, NO3−, AcO−, ClO4−, N3−, HCO3−) when measured in CH3CN/H2O (50 : 50, v/v) solution.
Weiying Lin - One of the best experts on this subject based on the ideXlab platform.
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Single Fluorescent Probe Separately and Continuously Visualize H2S and HClO in Lysosomes with Different Fluorescence Signals
2019Co-Authors: Mingguang Ren, Beibei Deng, Li Wang, Weiying LinAbstract:A complicated relationship between the active small molecules exists in cells. On the organelle level, active small molecules also play an important role in the maintenance of organelle functions and roles. To investigate the relationship of biomolecules in subcellular, it is necessary and critical to develop molecular tools that can track two kinds of associated biomolecules within organelles with multiple fluorescence signals. However, this is still an unmet challenge up to date. Herein, we present the first single-Fluorescent Probe (Lyso-HA-HS) that can detect oxidative (HOCl) and reductive (H2S) substances within an organelle (lysosomes) with multiresponse signals. The reactions of the new Probe with H2S and HOCl simultaneously result in the blue and red channels emissions, respectively, providing different signal responses to the oxidative and reductive substances in the cellular lysosomes. Using a single Fluorescent Probe, we first achieved dual-channel imaging of the endogenous hypochlorous acid and hydrogen sulfide, respectively, in the lysosomes in the living cells. Moreover, the highly desirable attributes of the Probe Lyso-HA-HS (such as high selectivity, good membrane-permeability, and lysosome enrichment ability) may enable it to be used in revealing the relationship of HOCl and H2S in lysosomes
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a two photon Fluorescent Probe for detecting lipid droplet viscosity in living cells and zebra fish
New Journal of Chemistry, 2018Co-Authors: Min Peng, Junling Yin, Weiying LinAbstract:The detection of lipid droplet (LD) viscosity is very important for clinical research. Herein, we described a new two-photon Fluorescent Probe CBA for sensing the viscosity changes of LDs. Using this powerful Probe, monitoring the viscosity variations has been achieved not only in cells but also in zebra fish.
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development of a near infrared Fluorescent Probe for monitoring hydrazine in serum and living cells
Analytical Methods, 2013Co-Authors: Sasa Zhu, Weiying Lin, Lin YuanAbstract:A new NIR Fluorescent Probe for monitoring hydrazine in blood samples and live cells was designed and synthesized.
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a ratiometric Fluorescent Probe for specific detection of cysteine over homocysteine and glutathione based on the drastic distinction in the kinetic profiles
Chemical Communications, 2011Co-Authors: Lin Yuan, Weiying Lin, Yueting YangAbstract:The first ratiometric Fluorescent Probe for specific detection of cysteine over homocysteine and glutathione was developed based on the drastic distinction in the kinetic profiles.
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a highly sensitive Fluorescent Probe for detection of benzenethiols in environmental samples and living cells
Chemical Communications, 2010Co-Authors: Weiying Lin, Lingliang Long, Wen TanAbstract:A highly sensitive Fluorescent Probe with a detection limit of 1.8 × 10−9 M was constructed and employed to detect benzenethiols in environmental samples and living cells.
Xiaojun Peng - One of the best experts on this subject based on the ideXlab platform.
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a nile blue based infrared Fluorescent Probe imaging tumors that over express cyclooxygenase 2
Chemical Communications, 2015Co-Authors: Benhua Wang, Jiangli Fan, Jingyun Wang, Xianwu Wang, Hao Zhu, Xiaojun PengAbstract:The first Golgi-localized cyclooxygenase-2 (COX-2)-specific near-infrared (NIR) Fluorescent Probe, Niblue-C6-IMC, able to detect cancer cells, was designed. Importantly, Niblue-C6-IMC preferentially labeled the tumors in a mouse tumor model with deep tissue penetration capacity. It may be a promising molecular tool for guiding tumor resection during surgery.
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naphthylamine rhodamine based ratiometric Fluorescent Probe for the determination of pd2 ions
Organic Letters, 2014Co-Authors: Shiguo Sun, Bo Qiao, Na Jiang, Jitao Wang, Si Zhang, Xiaojun PengAbstract:A naphthylamine-rhodamine hybrid ratiometric and colorimetric Fluorescent Probe (RN) was designed and synthesized. RN can identify Pd(2+) ions with high selectivity and sensitivity. Furthermore, the Probe can be used to monitor Pd(2+) ions in live mice by fluorescence imaging.
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a ratiometric near infrared Fluorescent Probe for hydrazine and its in vivo applications
Organic Letters, 2013Co-Authors: Wen Sun, Jianfang Cao, Jiangli Fan, Shiguo Sun, Jingyun Wang, Pan Gao, Fengling Song, Xiaojun PengAbstract:Based on modulation of the conjugated polymethine π-electron system of a cyanine dye derivative, a ratiometric near-infared Fluorescent Probe (Cy7A) for hydrazine (N2H4) has been designed and synthesized. Cy7A can be selectively hydrazinolysized with great changes in its Fluorescent excitation/emission profiles, which makes it possible to detect N2H4 in water samples and living cells and, for the first time, visualize N2H4 in living mice.
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A two-photon Fluorescent Probe with near-infrared emission for hydrogen sulfide imaging in biosystems
Chemical Communications, 2013Co-Authors: Wen Sun, Jianfang Cao, Jiangli Fan, Shiguo Sun, Chong Hu, Xiaoqing Xiong, Jingyun Wang, Shuang Cui, Hua Zhang, Xiaojun PengAbstract:Hydrogen sulfide (H2S) is emerging as an important gasotransmitter but remains difficult to study. Here we report a novel two-photon Fluorescent Probe with NIR emission for H2S detection. It was successfully used to realize H2S imaging in bovine serum, living cells, tissues as well as in living mice.
David R Spring - One of the best experts on this subject based on the ideXlab platform.
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a lysosome targetable Fluorescent Probe for imaging hydrogen sulfide in living cells
Organic Letters, 2013Co-Authors: Zhaochao Xu, David R SpringAbstract:In this work, a 1,8-naphthalimide-derived Fluorescent Probe for H2S based on the thiolysis of dinitrophenyl ether is reported. This Probe exhibits turn-on fluorescence detection of H2S in bovine serum and lysosome-targetable Fluorescent imaging of H2S with excellent selectivity.
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a selective and ratiometric cu2 Fluorescent Probe based on naphthalimide excimer monomer switching
Chemical Communications, 2010Co-Authors: Juyoung Yoo, David R SpringAbstract:A Fluorescent Probe was designed and shown to detect Cu2+ ratiometrically and selectively in aqueous solutions based on naphthalimide excimer–monomer switching.
Jianguo Fang - One of the best experts on this subject based on the ideXlab platform.
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a β allyl carbamate Fluorescent Probe for vicinal dithiol proteins
Chemical Communications, 2020Co-Authors: Lanning Zhao, Feifei Bai, Fan Chen, Menghuan Guo, Lu Gan, Hong Zhang, Jianguo FangAbstract:The first β-allyl carbamate Fluorescent Probe, AC-green, was reported for specifically imaging vicinal dithiol proteins (VDPs) in living systems. The Probe displays low toxicity and features high sensitivity and fast response towards VDPs. A drastic loss of VDPs in a Parkinson's model was disclosed by AC-green, linking the fluctuation of VDP levels to Parkinson's disease for the first time. The structural novelty and favorable properties of AC-green will advance the development of novel VDP Probes.
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A ratiometric Fluorescent Probe of methionine sulfoxide reductase with an improved response rate and emission wavelength.
Chemical Communications, 2019Co-Authors: Liangwei Zhang, Shoujiao Peng, Jianguo FangAbstract:A ratiometric Fluorescent Probe of methionine sulfoxide reductase, Msr-Ratio, was disclosed for monitoring the enzyme activity in vitro and in live cells. The Probe displayed favorable properties such as a nearly 400-fold fluorescence change, fast response rate (
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a ratiometric Fluorescent Probe of methionine sulfoxide reductase with an improved response rate and emission wavelength
Chemical Communications, 2019Co-Authors: Liangwei Zhang, Shoujiao Peng, Jianguo FangAbstract:A ratiometric Fluorescent Probe of methionine sulfoxide reductase, Msr-Ratio, was disclosed for monitoring the enzyme activity in vitro and in live cells. The Probe displayed favorable properties such as a nearly 400-fold fluorescence change, fast response rate (<30 min), large Stokes shift (120 nm), and green emission (550 nm).
