The Experts below are selected from a list of 89268 Experts worldwide ranked by ideXlab platform
Kai Johnsson - One of the best experts on this subject based on the ideXlab platform.
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small molecule Fluorescent Probes for live cell super resolution microscopy
Journal of the American Chemical Society, 2019Co-Authors: Lu Wang, Michelle S Frei, Aleksandar Salim, Kai JohnssonAbstract:Super-resolution fluorescence microscopy is a powerful tool to visualize biomolecules and cellular structures at the nanometer scale. Employing these techniques in living cells has opened up the possibility to study dynamic processes with unprecedented spatial and temporal resolution. Different physical approaches to super-resolution microscopy have been introduced over the last years. A bottleneck to apply these approaches for live-cell imaging has become the availability of appropriate Fluorescent Probes that can be specifically attached to biomolecules. In this Perspective, we discuss the role of small-molecule Fluorescent Probes for live-cell super-resolution microscopy and the challenges that need to be overcome for their generation. Recent trends in the development of labeling strategies are reviewed together with the required chemical and spectroscopic properties of the Probes. Finally, selected examples of the use of small-molecule Fluorescent Probes in live-cell super-resolution microscopy are given.
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small molecule Fluorescent Probes for live cell super resolution microscopy
Journal of the American Chemical Society, 2019Co-Authors: Lu Wang, Michelle S Frei, Aleksandar Salim, Kai JohnssonAbstract:Super-resolution fluorescence microscopy is a powerful tool to visualize biomolecules and cellular structures at the nanometer scale. Employing these techniques in living cells has opened up the possibility to study dynamic processes with unprecedented spatial and temporal resolution. Different physical approaches to super-resolution microscopy have been introduced over the last years. A bottleneck to apply these approaches for live-cell imaging has become the availability of appropriate Fluorescent Probes that can be specifically attached to biomolecules. In this Perspective, we discuss the role of small-molecule Fluorescent Probes for live-cell super-resolution microscopy and the challenges that need to be overcome for their generation. Recent trends in the development of labeling strategies are reviewed together with the required chemical and spectroscopic properties of the Probes. Finally, selected examples of the use of small-molecule Fluorescent Probes in live-cell super-resolution microscopy are given.
Tetsuo Nagano - One of the best experts on this subject based on the ideXlab platform.
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Small-molecule fluorophores and Fluorescent Probes for bioimaging
Pflügers Archiv - European Journal of Physiology, 2013Co-Authors: Takuya Terai, Tetsuo NaganoAbstract:Fluorescent compounds based on synthetic small molecules are powerful tools to visualize biological events in living cells and organisms. Ever since the discovery of organic Fluorescent compounds in the late nineteenth century, efforts have been made to “see” the behaviors of specific biomolecules in living systems by using these dyes as labels. Also, following the development of Fluorescent Ca^2+ indicators in the 1980s, many Fluorescent Probes or biosensors, which are defined as molecules that show a change in fluorescence properties in the presence of their target molecule, have been reported and applied in biological research. Today, a variety of Probes are available that target metal ions, pH, enzyme activities, and signaling molecules. In this review, we first consider the history of organic Fluorescent molecules and discuss their utility for labeling biomolecules and staining cells. Then, we review recent progress in small-molecule Fluorescent Probes for metal ions and reactive oxygen species, focusing on representative work in each category. Finally, we briefly discuss attempts to create novel kinds of Probes, including hybrids of small molecules and genetically encoded proteins, with the potential to overcome some of the limitations of current Probes.
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Fluorescent Probes for sensing and imaging
Nature Methods, 2011Co-Authors: Tasuku Ueno, Tetsuo NaganoAbstract:A diverse array of small molecule–based Fluorescent Probes is available for many different types of biological experiments. Here we examine the history of these Probes and discuss some of the most interesting applications.
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Development of Fluorescent Probes for bioimaging applications.
Proceedings of the Japan Academy. Series B Physical and biological sciences, 2010Co-Authors: Tetsuo NaganoAbstract:: Fluorescent Probes, which allow visualization of cations such as Ca(2+), Zn(2+) etc., small biomolecules such as nitric oxide (NO) or enzyme activities in living cells by means of fluorescence microscopy, have become indispensable tools for clarifying functions in biological systems. This review deals with the general principles for the design of bioimaging Fluorescent Probes by modulating the fluorescence properties of fluorophores, employing mechanisms such as acceptor-excited Photoinduced electron Transfer (a-PeT), donor-excited Photoinduced electron Transfer (d-PeT), and spirocyclization, which have been established by our group. The a-PeT and d-PeT mechanisms are widely applicable for the design of bioimaging Probes based on many fluorophores and the spirocyclization process is also expected to be useful as a fluorescence off/on switching mechanism. Fluorescence modulation mechanisms are essential for the rational design of novel fluorescence Probes for target molecules. Based on these mechanisms, we have developed more than fifty bioimaging Probes, of which fourteen are commercially available. The review also describes some applications of the Probes developed by our group to in vitro and in vivo systems.
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Fluorescent Probes for bioimaging applications
Current Opinion in Chemical Biology, 2008Co-Authors: Takuya Terai, Tetsuo NaganoAbstract:Fluorescent Probes based on small organic molecules have become indispensable tools in modern biology because they provide dynamic information concerning the localization and quantity of the molecules of interest, without the need of genetic engineering of the sample. In this review, following a brief outline of the principle of fluorescence imaging, we recount some recent achievements in the field of small-molecular Fluorescent Probes. First, Probes for metal cations, including those suitable for two-photon imaging, are introduced. Next, methodologies to visualize proteases are discussed, with special emphasis on activity-based Probes for use in vivo. All these Probes have been confirmed to be applicable to cellular or in vivo imaging.
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functional near infrared Fluorescent Probes
Chemistry-an Asian Journal, 2008Co-Authors: Kazuki Kiyose, Hirotatsu Kojima, Tetsuo NaganoAbstract:Near-infrared (NIR) Fluorescent Probes have attracted much attention, but despite the availability of various NIR fluorophores, only a few functional NIR Probes, that is, Probes whose absorption and/or fluorescence spectra change upon specific reaction with biomolecules, have been developed. However, functional Probes operating in the NIR range that can be targeted to protons, metal ions, nitric oxide, β-galactosidase, and cellular stress markers are expected to be effective for fluorescence imaging in vivo. This Focus Review concentrates on these functional NIR Probes themselves, not their applications.
Juyoung Yoon - One of the best experts on this subject based on the ideXlab platform.
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Synthetic ratiometric Fluorescent Probes for detection of ions
Chemical Society reviews, 2020Co-Authors: Sanghyun Park, Nahyun Kwon, Jee-hyeon Lee, Juyoung Yoon, Injae ShinAbstract:Metal cations and anions are essential for versatile physiological processes. Dysregulation of specific ion levels in living organisms is known to have an adverse effect on normal biological events. Owing to the pathophysiological significance of ions, sensitive and selective methods to detect these species in biological systems are in high demand. Because they can be used in methods for precise and quantitative analysis of ions, organic dye-based ratiometric Fluorescent Probes have been extensively explored in recent years. In this review, recent advances (2015-2019) made in the development and biological applications of synthetic ratiometric Fluorescent Probes are described. Particular emphasis is given to organic dye-based ratiometric Fluorescent Probes that are designed to detect biologically important and relevant ions in cells and living organisms. Also, the fundamental principles associated with the design of ratiometric Fluorescent Probes and perspectives about how to expand their biological applications are discussed.
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Fluorescent Probes Containing Selenium as a Guest or Host
Chem, 2016Co-Authors: Liyan Chen, Nahyun Kwon, Juyoung YoonAbstract:Summary Selenium is an important trace element in living systems. Proteins incorporating selenium have a wide range of biological effects, ranging from anti-oxidant and anti-inflammatory effects to the production of active thyroid hormones. In the past decades, selenium has drawn enormous attention because of its important role in biology, which can be ascribed partly if not solely to its redox and hard and soft donor properties. With the use of chemical mimicry, a large number of selenium-related Fluorescent Probes have been developed for monitoring physiological and pathological processes. This review, which summarizes recent progress made in this area, comprises two major sections. In the first section, Fluorescent Probes for selenium-containing species, such as selenocysteine (Sec), hydrogen selenide, thioredoxin reductases (TrxRs), and selenite, are described. This is followed by a discussion of Fluorescent Probes that contain selenium and detect reactive oxygen, nitrogen, and sulfur species, as well as cations and anions.
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development of Fluorescent Probes based on protection deprotection of the key functional groups for biological imaging
Chemical Society Reviews, 2015Co-Authors: Yonghe Tang, Weiying Lin, Dayoung Lee, Jiaoliang Wang, Juyoung YoonAbstract:Recently, the strategy of protection–deprotection of functional groups has been widely employed to design Fluorescent Probes, as the protection–deprotection of functional groups often induces a marked change in electronic properties. Significant advances have been made in the development of analyte-responsive Fluorescent Probes based on the protection–deprotection strategy. In this tutorial review, we highlight the representative examples of small-molecule based Fluorescent Probes for bioimaging, which are operated via the protection–deprotection of key functional groups such as aldehyde, hydroxyl, and amino functional groups reported from 2010 to 2014. The discussion includes the general protection–deprotection methods for aldehyde, hydroxyl, or amino groups, as well as the design strategies, sensing mechanisms, and deprotection modes of the representative Fluorescent imaging Probes applied to bio-imaging.
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Fluorescent Probes and bioimaging alkali metals alkaline earth metals and ph
Chemical Society Reviews, 2015Co-Authors: Ying Hu, Juyoung YoonAbstract:All living species and life forms have an absolute requirement for bio-functional metals and acid–base equilibrium chemistry owing to the critical roles they play in biological processes. Hence, a great need exists for efficient methods to detect and monitor biometals and acids. In the last few years, great attention has been paid to the development of organic molecule based Fluorescent chemosensors. The availability of new synthetic Fluorescent Probes has made fluorescence microscopy an indispensable tool for tracing biologically important molecules and in the area of clinical diagnostics. This review highlights the recent advances that have been made in the design and bioimaging applications of Fluorescent Probes for alkali metals and alkaline earth metal cations, including lithium, sodium and potassium, magnesium and calcium, and for pH determination within biological systems.
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recent progress in the development of near infrared Fluorescent Probes for bioimaging applications
Chemical Society Reviews, 2014Co-Authors: Sookil Park, Juyoung Yoon, Injae ShinAbstract:Near-infrared (NIR) Fluorescent dyes have emerged as promising modalities for monitoring the levels of various biologically relevant species in cells and organisms. The use of NIR Probes enables deep photon penetration in tissue, minimizes photo-damage to biological samples, and produces low background auto-fluorescence from biomolecules present in living systems. The number of new analyte-responsive NIR Fluorescent Probes has increased substantially in recent years as a consequence of intense research efforts. In this tutorial review, we highlight recent advances (2010–2013) made in the development and applications of NIR Fluorescent Probes. The review focuses on NIR Fluorescent Probes that have been devised to sense various biologically important species, including ROS/RNS, metal ions, anions, enzymes and other related species, as well as intracellular pH changes. The basic principles involved in the design of functional NIR Fluorescent Probes and suggestions about how to expand applications of NIR imaging agents are also described.
Aleksandar Salim - One of the best experts on this subject based on the ideXlab platform.
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small molecule Fluorescent Probes for live cell super resolution microscopy
Journal of the American Chemical Society, 2019Co-Authors: Lu Wang, Michelle S Frei, Aleksandar Salim, Kai JohnssonAbstract:Super-resolution fluorescence microscopy is a powerful tool to visualize biomolecules and cellular structures at the nanometer scale. Employing these techniques in living cells has opened up the possibility to study dynamic processes with unprecedented spatial and temporal resolution. Different physical approaches to super-resolution microscopy have been introduced over the last years. A bottleneck to apply these approaches for live-cell imaging has become the availability of appropriate Fluorescent Probes that can be specifically attached to biomolecules. In this Perspective, we discuss the role of small-molecule Fluorescent Probes for live-cell super-resolution microscopy and the challenges that need to be overcome for their generation. Recent trends in the development of labeling strategies are reviewed together with the required chemical and spectroscopic properties of the Probes. Finally, selected examples of the use of small-molecule Fluorescent Probes in live-cell super-resolution microscopy are given.
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small molecule Fluorescent Probes for live cell super resolution microscopy
Journal of the American Chemical Society, 2019Co-Authors: Lu Wang, Michelle S Frei, Aleksandar Salim, Kai JohnssonAbstract:Super-resolution fluorescence microscopy is a powerful tool to visualize biomolecules and cellular structures at the nanometer scale. Employing these techniques in living cells has opened up the possibility to study dynamic processes with unprecedented spatial and temporal resolution. Different physical approaches to super-resolution microscopy have been introduced over the last years. A bottleneck to apply these approaches for live-cell imaging has become the availability of appropriate Fluorescent Probes that can be specifically attached to biomolecules. In this Perspective, we discuss the role of small-molecule Fluorescent Probes for live-cell super-resolution microscopy and the challenges that need to be overcome for their generation. Recent trends in the development of labeling strategies are reviewed together with the required chemical and spectroscopic properties of the Probes. Finally, selected examples of the use of small-molecule Fluorescent Probes in live-cell super-resolution microscopy are given.
Michelle S Frei - One of the best experts on this subject based on the ideXlab platform.
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small molecule Fluorescent Probes for live cell super resolution microscopy
Journal of the American Chemical Society, 2019Co-Authors: Lu Wang, Michelle S Frei, Aleksandar Salim, Kai JohnssonAbstract:Super-resolution fluorescence microscopy is a powerful tool to visualize biomolecules and cellular structures at the nanometer scale. Employing these techniques in living cells has opened up the possibility to study dynamic processes with unprecedented spatial and temporal resolution. Different physical approaches to super-resolution microscopy have been introduced over the last years. A bottleneck to apply these approaches for live-cell imaging has become the availability of appropriate Fluorescent Probes that can be specifically attached to biomolecules. In this Perspective, we discuss the role of small-molecule Fluorescent Probes for live-cell super-resolution microscopy and the challenges that need to be overcome for their generation. Recent trends in the development of labeling strategies are reviewed together with the required chemical and spectroscopic properties of the Probes. Finally, selected examples of the use of small-molecule Fluorescent Probes in live-cell super-resolution microscopy are given.
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small molecule Fluorescent Probes for live cell super resolution microscopy
Journal of the American Chemical Society, 2019Co-Authors: Lu Wang, Michelle S Frei, Aleksandar Salim, Kai JohnssonAbstract:Super-resolution fluorescence microscopy is a powerful tool to visualize biomolecules and cellular structures at the nanometer scale. Employing these techniques in living cells has opened up the possibility to study dynamic processes with unprecedented spatial and temporal resolution. Different physical approaches to super-resolution microscopy have been introduced over the last years. A bottleneck to apply these approaches for live-cell imaging has become the availability of appropriate Fluorescent Probes that can be specifically attached to biomolecules. In this Perspective, we discuss the role of small-molecule Fluorescent Probes for live-cell super-resolution microscopy and the challenges that need to be overcome for their generation. Recent trends in the development of labeling strategies are reviewed together with the required chemical and spectroscopic properties of the Probes. Finally, selected examples of the use of small-molecule Fluorescent Probes in live-cell super-resolution microscopy are given.