The Experts below are selected from a list of 24384 Experts worldwide ranked by ideXlab platform

Yasunori Yoshimura - One of the best experts on this subject based on the ideXlab platform.

Emily S. Shen - One of the best experts on this subject based on the ideXlab platform.

  • 5 alkylated thiazolidinones as Follicle Stimulating Hormone fsh receptor agonists
    Bioorganic & Medicinal Chemistry, 2006
    Co-Authors: Jay E. Wrobel, John A. Rogers, James W Jetter, Wenling Kao, Claudia M Perez, Jamin Chi, Gichung Chen, Emily S. Shen
    Abstract:

    Abstract We prepared analogs of potent thiazolidinone-based Follicle-Stimulating Hormone (FSH) agonists 1 , that is, 3 that contained an additional 5-alkyl substituent. This extra substituent was added to reduce synthetic problems that arose during preparation of analogs of 1 . These compounds ( 3 ) were evaluated in a Chinese hamster ovary (CHO) cell line that expressed recombinant human FSH receptor (FSHR) and a luciferase reporter gene regulated by a cAMP response element (CRE). Selected compounds were also tested on a CHO-cell line that over expressed the FSHR for the ability to induce cAMP production. When the 5-alkyl substituent was a methyl group as in analog 16a , similar FSH activity (i.e., EC 50  = 51 nM, 100% efficacy relative to hFSH) to the analogous 5-hydrogen series compound (e.g., 2 ) was observed; thus, proving that a small 5-alkyl substituent was well tolerated. New derivatives of 3 , in which the potentially hydrolytically labile secondary amide function of 1 (–CONH–) was modified to other moieties (e.g., –CH 2 NH–, –CH 2 S–, and –CH 2 OCONH–), were also prepared and evaluated. These congeners (namely 21 , 22 , and 24 ) also displayed good potency in the CRE-luciferase assay.

  • Preparation of highly substituted γ-lactam Follicle Stimulating Hormone receptor agonists
    Bioorganic & Medicinal Chemistry, 2005
    Co-Authors: Jeffrey C. Pelletier, John A. Rogers, Jay E. Wrobel, M. Claudia Perez, Emily S. Shen
    Abstract:

    An unusual combination of Weinreb amidation and Mitsunobu lactam formation was used to prepare highly substituted γ-lactam analogues of a thiazolidinone Follicle Stimulating Hormone receptor agonist. The analogue synthesis was stereoselective and the final products were chemically stable. Biological properties of the target molecules were nearly identical to those of the lead compound.

Salim Daya - One of the best experts on this subject based on the ideXlab platform.

  • cost effectiveness of recombinant versus urinary Follicle Stimulating Hormone in assisted reproduction techniques in the spanish public health care system
    Journal of Assisted Reproduction and Genetics, 2003
    Co-Authors: Alberto Romeu, Juan Balasch, Jose Ruiz A Balda, Pedro N Barri, Salim Daya, Jean Paul Auray, G Duru, Ariel Beresniak, Jose A Peinado
    Abstract:

    Purpose: To evaluate the relative cost-effectiveness of recombinant and urinary Follicle-Stimulating Hormone (FSH) in assisted reproduction techniques in the Spanish National Health Service.

  • recombinant versus urinary Follicle Stimulating Hormone for ovarian stimulation in assisted reproduction
    Human Reproduction, 1999
    Co-Authors: Salim Daya, Joanne Gunby
    Abstract:

    The recent availability of recombinant Follicle Stimulating Hormone (rFSH), with its high level of purity and batch-to-batch consistency has made it an attractive alternative to urinary FSH (uFSH) for ovarian stimulation. Several trials have compared the two preparations, but none had sufficient power to detect a clinically meaningful difference in pregnancy rates. The purpose of this study was to determine the clinical pregnancy rates per started cycle by pooling data from randomized trials which compared the use of rFSH and uFSH in treatment cycles using in-vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). A thorough search of the literature identified 12 trials which met the inclusion criteria. In four trials, both IVF and ICSI were performed, in seven trials only IVF was performed and in one trial only ICSI was performed. Data were extracted and pooled using the principles of meta-analysis. There was no significant heterogeneity of treatment effect across the trials. The common odds ratio and the risk difference (and their 95% confidence intervals), obtained by pooling the data using a fixed effects model, were 1.20 (1.02-1.42) and 3.7% (0.5-6.9%) respectively, in favour of rFSH. The pregnancy rate with the alpha preparation of rFSH was statistically significantly higher than with uFSH in IVF cycles. The overall conclusion from this meta-analysis is that the use of rFSH in assisted reproduction is preferred over uFSH.

Joanne Gunby - One of the best experts on this subject based on the ideXlab platform.

  • recombinant versus urinary Follicle Stimulating Hormone for ovarian stimulation in assisted reproduction
    Human Reproduction, 1999
    Co-Authors: Salim Daya, Joanne Gunby
    Abstract:

    The recent availability of recombinant Follicle Stimulating Hormone (rFSH), with its high level of purity and batch-to-batch consistency has made it an attractive alternative to urinary FSH (uFSH) for ovarian stimulation. Several trials have compared the two preparations, but none had sufficient power to detect a clinically meaningful difference in pregnancy rates. The purpose of this study was to determine the clinical pregnancy rates per started cycle by pooling data from randomized trials which compared the use of rFSH and uFSH in treatment cycles using in-vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). A thorough search of the literature identified 12 trials which met the inclusion criteria. In four trials, both IVF and ICSI were performed, in seven trials only IVF was performed and in one trial only ICSI was performed. Data were extracted and pooled using the principles of meta-analysis. There was no significant heterogeneity of treatment effect across the trials. The common odds ratio and the risk difference (and their 95% confidence intervals), obtained by pooling the data using a fixed effects model, were 1.20 (1.02-1.42) and 3.7% (0.5-6.9%) respectively, in favour of rFSH. The pregnancy rate with the alpha preparation of rFSH was statistically significantly higher than with uFSH in IVF cycles. The overall conclusion from this meta-analysis is that the use of rFSH in assisted reproduction is preferred over uFSH.

Abigail Elizur - One of the best experts on this subject based on the ideXlab platform.

  • In-vitro and in-vivo biological activity of recombinant yellowtail kingfish (Seriola lalandi) Follicle Stimulating Hormone
    General and Comparative Endocrinology, 2016
    Co-Authors: Pablo J. Sanchís-benlloch, Joseph Aizen, Adam Miller, Michal Shpilman, Tomer Ventura, Josephine Nocillado, Berta Levavi-sivan, Claudia Ladisa, Abigail Elizur
    Abstract:

    Biologically active recombinant yellowtail kingfish Follicle Stimulating Hormone (rytkFsh) was produced in yeast Pichia pastoris and its biological activity was demonstrated by both in-vitro and in-vivo bioassays. Incubation of ovarian and testicular fragments with the recombinant Hormone stimulated E2 and 11-KT secretion, respectively. In-vivo trial in immature female YTK resulted in a significant increase of plasma E2 levels and development of oocytes. In males at the early stages of puberty, advancement of spermatogenesis was observed, however plasma 11-KT levels were reduced when administered with rytkFsh.