The Experts below are selected from a list of 312 Experts worldwide ranked by ideXlab platform
Hajime Miyamoto - One of the best experts on this subject based on the ideXlab platform.
-
Changes in Localization of Type I, III and IV Collagens and Their mRNA Expression During Follicular Atresia in Bovine and Porcine Ovaries
Journal of Reproduction and Development, 2020Co-Authors: Mizuho Nakayama, Noboru Manabe, Kozue Yamada-uchio, Hajime MiyamotoAbstract:Determination of the expression level and localization of extracellular matrix (ECM) components is crucial for understanding the mechanism of maintenance and remodeling of the ovarian follicle structure. Previously, we demonstrated species-specific differences in the process of apoptosis in granulosa and theca cells during Follicular Atresia. In the present study, we histochemically compared the localization of type I, III and IV collagens and the expression of their mRNA. In healthy porcine or bovine follicles, type III and IV collagens were mainly distributed in theca interna layers, and strong to moderate expression of their mRNAs were observed in granulosa and theca interna layers, respectively. During Follicular Atresia, marked decreases in type I and IV collagens were observed in porcine follicles, while no changes were seen in bovine follicles. These findings indicate that these ECMs have important roles in Follicular development and/or degeneration, and that species-specific differences in their production reflect differences in the regulatory mechanism of granulosa cell apoptosis between atretic porcine and bovine follicles.
-
The Ovary (Second Edition) - CHAPTER 21 – Follicle Selection in Mammalian Ovaries: Regulatory Mechanisms of Granulosa Cell Apoptosis during Follicular Atresia
The Ovary, 2020Co-Authors: Noboru Manabe, Hajime Miyamoto, Naoke Inoue, Miki Sugimoto, Takashi Miyano, Kazuhiro SakamakiAbstract:This chapter focuses on the follicle selection that is regulated by discriminating Atresia of growing follicles and describes regulation factors that affect the Follicular Atresia. It briefs describes some of the factors which might regulate Atresia or Follicular development and observations that suggest a novel cell-death receptor and decoy receptor in regulating follicle selection in porcine ovaries. Apoptosis is the considered as the mechanism underlying ovarian Follicular Atresia. Specific apoptosis-related genes, such as caspase-3 and apoptotic protease activating factor-1, express in granulosa cells of atretic follicles specific changes in glycoconjugates of cell surface membrane-glycoprotein, which act as regulators for phagocytosis in neighboring granulose cells in atretic follicles. Expression levels of kinase cascades, which regulate regulating entry into cell death and proliferation in granulosa cells, change during Atresia. Studies have reported that the degeneration of atretic follicles in mammalian ovaries can be explained, at least in part, by apoptotic cell death of granulosa cells and endocrine cells of the theca interna layer. Histological, cytological, and biochemical examination of the degenerating changes cumulus cells during Follicular Atresia in porcine ovaries have confirmed that apoptosis occurs in granulosa cells but not cumulus cells or oocytes in tertiary follicles in the early stage of Atresia.
-
expression and activity of apaf1 and caspase 9 in granulosa cells during Follicular Atresia in pig ovaries
Reproduction, 2003Co-Authors: T Matsui, Naoko Inoue, N Manabe, Y Goto, S Nishihara, Hajime MiyamotoAbstract:: Apoptosis in granulosa cells plays a crucial role in ovarian Follicular Atresia, but the intracellular regulating mechanism, especially the mitochondrion-dependent apoptosis signalling pathway, is still largely unknown. This study examined whether the mitochondrial pathway is associated with granulosa cell apoptosis during Atresia in pig ovaries. Both mRNAs of caspase-9 and apoptotic protease-activating factor 1 (Apaf1), which are major signal transducing components in the mitochondrial pathway, were detected in granulosa cells in healthy, early atretic and progressed atretic follicles by RT-PCR. No changes in the expression of Apaf1 mRNA were seen during Follicular Atresia, but the expression of caspase-9 mRNA increased during Atresia. Apaf1 protein was steadily detected in granulosa cells prepared from healthy, early atretic and progressed atretic follicles by western blot analysis, but high expression of the precursor of caspase-9 (procaspase-9) was detected only in granulosa cells of healthy follicles. Decreased procaspase-9 protein was demonstrated during Follicular Atresia. Proteolytic activity of caspase-9 increased during Atresia, in agreement with the diminution of procaspase-9 protein. Intensive expression of caspase-9 mRNA was demonstrated in the granulosa cells of early atretic and progressed atretic follicles but not in those of healthy follicles. These results indicate that the mitochondrial signalling pathway, which is mediated by Apaf1 and caspase-9, plays a crucial role in determining the fate of granulosa cells during Atresia in pig ovaries.
-
chapter 21 follicle selection in mammalian ovaries regulatory mechanisms of granulosa cell apoptosis during Follicular Atresia
The Ovary (Second Edition), 2003Co-Authors: Noboru Manabe, Hajime Miyamoto, Naoke Inoue, Miki Sugimoto, Takashi Miyano, Kazuhiro SakamakiAbstract:This chapter focuses on the follicle selection that is regulated by discriminating Atresia of growing follicles and describes regulation factors that affect the Follicular Atresia. It briefs describes some of the factors which might regulate Atresia or Follicular development and observations that suggest a novel cell-death receptor and decoy receptor in regulating follicle selection in porcine ovaries. Apoptosis is the considered as the mechanism underlying ovarian Follicular Atresia. Specific apoptosis-related genes, such as caspase-3 and apoptotic protease activating factor-1, express in granulosa cells of atretic follicles specific changes in glycoconjugates of cell surface membrane-glycoprotein, which act as regulators for phagocytosis in neighboring granulose cells in atretic follicles. Expression levels of kinase cascades, which regulate regulating entry into cell death and proliferation in granulosa cells, change during Atresia. Studies have reported that the degeneration of atretic follicles in mammalian ovaries can be explained, at least in part, by apoptotic cell death of granulosa cells and endocrine cells of the theca interna layer. Histological, cytological, and biochemical examination of the degenerating changes cumulus cells during Follicular Atresia in porcine ovaries have confirmed that apoptosis occurs in granulosa cells but not cumulus cells or oocytes in tertiary follicles in the early stage of Atresia.
-
Changes in Cell Adhesion Molecules during Follicular Atresia in Porcine Ovaries
Journal of Reproduction and Development, 2001Co-Authors: Susumu Nishihara, Noboru Manabe, Naoko Inoue, Mizuho Nakayama, Satoko Wada, Hajime MiyamotoAbstract:Determination of the expression level and localization of cell adhesion molecules is crucial for understanding the mechanism of maintenance and remodeling of the ovarian follicle structure. We immunocytochemically investigated expression of the cell adhesion molecules, "classic" cadherins and β-catenin, in developing and/or atretic follicles of porcine ovaries. Healthy follicles showed strong staining for cadherin-8 and β-catenin in granulosa cells tightly attached to the basement membrane, and moderate/weak staining was seen on the inner surface of the granulosa cell layer. Strong VE-cadherin expression was seen in a single cell layer attached to the basement membrane in the theca interna layer of healthy follicles. The expression of cadherin-8, β-catenin and VE-cadherin decreased during Follicular Atresia. No positive staining was observed for R-cadherin, E-cadherin, T-cadherin, BR-cadherin or P-cadherin, and a weak positive reaction for N-cadherin was seen only in the granulosa cells of healthy and early atretic follicles. These findings indicate that cadherin-8, N-cadherin and VE-cadherin have important roles in Follicular development and/or degeneration, and that decreases in the expression of these cadherins are involved in Follicular Atresia in porcine follicles.
Elizete Rizzo - One of the best experts on this subject based on the ideXlab platform.
-
Autophagy and Cathepsin D mediated apoptosis contributing to ovarian Follicular Atresia in the Nile tilapia.
Molecular Reproduction and Development, 2019Co-Authors: Camila Ferreira Sales, Nilo Bazzoli, Rafael Magno Costa Melo, Ana Paula Barbosa Pinheiro, Elizete RizzoAbstract:: Follicular Atresia is a hormonally controlled degenerative process involving apoptosis of the somatic and germ cells. Since different signaling pathways can induce cell death, the aim of the present study was to investigate cell death signaling and crosstalk between autophagic, apoptotic, and lysosomal proteins during Follicular Atresia in Nile tilapia. For this, females were kept in controlled conditions for 21 days, and ovary samples were collected weekly. The atretic follicles (AF) were analyzed in three regression phases: Early, advanced, and late. Under electron microscopy, the Follicular cells exhibited numerous protein synthesis organelles in the early AF. Immunoreactivity for Bcl2, Beclin1, Lc3, and Cathepsin D increased significantly in advanced AF (p
-
autophagy and cathepsin d mediated apoptosis contributing to ovarian Follicular Atresia in the nile tilapia
Molecular Reproduction and Development, 2019Co-Authors: Camila Ferreira Sales, Nilo Bazzoli, Rafael Magno Costa Melo, Ana Paula Barbosa Pinheiro, Elizete RizzoAbstract:Follicular Atresia is a hormonally controlled degenerative process involving apoptosis of the somatic and germ cells. Since different signaling pathways can induce cell death, the aim of the present study was to investigate cell death signaling and crosstalk between autophagic, apoptotic, and lysosomal proteins during Follicular Atresia in Nile tilapia. For this, females were kept in controlled conditions for 21 days, and ovary samples were collected weekly. The atretic follicles (AF) were analyzed in three regression phases: Early, advanced, and late. Under electron microscopy, the Follicular cells exhibited numerous protein synthesis organelles in the early AF. Immunoreactivity for Bcl2, Beclin1, Lc3, and Cathepsin D increased significantly in advanced AF (p < .001), when Follicular cells were in intense yolk phagocytosis. In this phase, autophagosomes and autolysosomes were frequently observed. In the late AF, Follicular cells had a markedly electron-lucid cytoplasm and immunoreactivity for Bax and TUNEL assay indicated an elevated apoptosis rate. Colocalisation of Lamp1/Cathepsin D and Lc3/Caspase-3 suggests dynamic crosstalk between the autophagy, apoptosis, and lysosome pathways. Taken together, the data indicate that autophagy plays a role in the homeostasis and clearance of the Follicular cells preceding Cathepsin D mediated apoptosis during Follicular Atresia in Nile tilapia.
-
autophagy and apoptosis interplay during Follicular Atresia in fish ovary a morphological and immunocytochemical study
Cell and Tissue Research, 2012Co-Authors: Roberto D V S Morais, Nilo Bazzoli, Ralph Gruppi Thome, F S Lemos, Elizete RizzoAbstract:Follicular Atresia in fish ovary provides an interesting model for studying autophagy and apoptosis. In order to improve knowledge of the mechanisms regulating ovarian regression, we investigated the immunolocalisation of various proteins involved in the complex network of autophagy and apoptosis. Females of three species of freshwater fish maintained in captivity were sampled after the reproductive period and the main events of Follicular Atresia were assessed by histology: splits in the zona radiata, yolk degradation and reabsorption, hypertrophy of the Follicular cells, accumulation of autophagic vacuoles, closing of the Follicular lumen and thickening of the theca. The interplay of apoptosis and autophagy was analysed by TUNEL in situ and by immunocytochemistry for caspase-3, bax, bcl-2, beclin-1 and cathepsin-D. During early and advanced stages of Follicular regression, the actin cytoskeleton was well developed and labelling for bcl-2 and cathepsin-D were pronounced in the Follicular cells at a stage when they were intensively involved in yolk phagocytosis. Immunofluorescence for beclin-1 was prevalent in the Follicular cells, punctate labelling often surrounding autophagic vacuoles during the advanced stage of Follicular regression, a critical step towards cell death. TUNEL-positive reaction and immunostaining for bax and caspase-3 demonstrated the participation of apoptosis in late Follicular regression. Overall, this study provides evidence that autophagic and apoptotic proteins are activated in a coordinated fashion depending on the stage of Follicular regression, with interplay between autophagy and apoptosis being essential in determining the fate of the cell during Follicular Atresia in fish ovary.
-
dual roles for autophagy during Follicular Atresia in fish ovary
Autophagy, 2009Co-Authors: Ralph Gruppi Thome, Nilo Bazzoli, Helio Batista Dos Santos, Fabio Pereira Arantes, Fabricio F T Domingos, Elizete RizzoAbstract:Autophagy, a highly conserved catabolic program for degrading proteins and organelles, is essential for cell and tissue homeostasis. Primarily, this process has a cytoprotective role under nutrient deprivation, but several stress stimuli can induce autophagy and, thus, distinct programmed cell death (PCD) pathways can be actived when stress is not abolished. Fish ovaries are a suitable experimental model system for studying the mechanisms of PCD due to the presence of postovulatory and atretic (i.e., non-ovulated) follicles, which follow different routes after spawning. Apoptosis of the Follicular cells is the major mechanism responsible for the rapid resorption of the postovulatory follicles. Recently, we investigated the contribution of PCD during Follicular Atresia in two species of freshwater fish. In contrast to mammals, this study revealed that Follicular apoptosis is not a major process for deletion of Follicular cells in atretic follicles. Furthermore, we detected autophagic vacuoles containing de...
-
ovarian Follicular Atresia is mediated by heterophagy autophagy and apoptosis in prochilodus argenteus and leporinus taeniatus teleostei characiformes
Theriogenology, 2008Co-Authors: Helio Batista Dos Santos, Nilo Bazzoli, Yoshimi Sato, Ralph Gruppi Thome, Fabio Pereira Arantes, Elizete RizzoAbstract:We investigated apoptosis, cell proliferation antigen (PCNA), and heat shock protein (HSP70) during ovarian Follicular Atresia in two freshwater teleost species from the Sao Francisco River basin, Brazil: curimata-pacu, Prochilodus argenteus and piau-jejo, Leporinus taeniatus. Fishes were maintained in captivity after the reproductive period and ovarian regression was assessed by gonadosomatic index for three stages: early, advanced, and late regression. Follicular Atresia was analysed by light and transmission electron microscopy, as well as by TUNEL and immunohistochemistry for HSP70 and PCNA. During early regression, atretic follicles exhibited zona pellucida breakdown, yolk degeneration, and hypertrophied Follicular cells (e.g. granulosa in mammals). Intense heterophagy to engulf the yolk, and autophagy were detected in the Follicular cells during advanced and late Atresia. The TUNEL assay detected DNA fragmentation, mainly in late Follicular Atresia. The apoptosis rate of the Follicular cells increased up to 10% during Follicular Atresia in both species and was negatively correlated with Follicular area. Immunohistochemistry reaction for HSP70 stained the Follicular cells strongly during advanced Atresia, when they are intensively involved in yolk engulfment, whereas the reaction for PCNA labelled theca cells. We inferred that heterophagy, autophagy, and apoptosis contributed to Follicular Atresia in teleost ovaries, thereby achieving a more efficient removal of the degenerating oocyte and dying Follicular cells. Additionally, HSP70 may protect the Follicular cells before apoptosis when they are involved in yolk engulfment, and cell proliferation in the theca contributed to ovarian remodelling.
Noboru Manabe - One of the best experts on this subject based on the ideXlab platform.
-
Changes in Localization of Type I, III and IV Collagens and Their mRNA Expression During Follicular Atresia in Bovine and Porcine Ovaries
Journal of Reproduction and Development, 2020Co-Authors: Mizuho Nakayama, Noboru Manabe, Kozue Yamada-uchio, Hajime MiyamotoAbstract:Determination of the expression level and localization of extracellular matrix (ECM) components is crucial for understanding the mechanism of maintenance and remodeling of the ovarian follicle structure. Previously, we demonstrated species-specific differences in the process of apoptosis in granulosa and theca cells during Follicular Atresia. In the present study, we histochemically compared the localization of type I, III and IV collagens and the expression of their mRNA. In healthy porcine or bovine follicles, type III and IV collagens were mainly distributed in theca interna layers, and strong to moderate expression of their mRNAs were observed in granulosa and theca interna layers, respectively. During Follicular Atresia, marked decreases in type I and IV collagens were observed in porcine follicles, while no changes were seen in bovine follicles. These findings indicate that these ECMs have important roles in Follicular development and/or degeneration, and that species-specific differences in their production reflect differences in the regulatory mechanism of granulosa cell apoptosis between atretic porcine and bovine follicles.
-
The Ovary (Second Edition) - CHAPTER 21 – Follicle Selection in Mammalian Ovaries: Regulatory Mechanisms of Granulosa Cell Apoptosis during Follicular Atresia
The Ovary, 2020Co-Authors: Noboru Manabe, Hajime Miyamoto, Naoke Inoue, Miki Sugimoto, Takashi Miyano, Kazuhiro SakamakiAbstract:This chapter focuses on the follicle selection that is regulated by discriminating Atresia of growing follicles and describes regulation factors that affect the Follicular Atresia. It briefs describes some of the factors which might regulate Atresia or Follicular development and observations that suggest a novel cell-death receptor and decoy receptor in regulating follicle selection in porcine ovaries. Apoptosis is the considered as the mechanism underlying ovarian Follicular Atresia. Specific apoptosis-related genes, such as caspase-3 and apoptotic protease activating factor-1, express in granulosa cells of atretic follicles specific changes in glycoconjugates of cell surface membrane-glycoprotein, which act as regulators for phagocytosis in neighboring granulose cells in atretic follicles. Expression levels of kinase cascades, which regulate regulating entry into cell death and proliferation in granulosa cells, change during Atresia. Studies have reported that the degeneration of atretic follicles in mammalian ovaries can be explained, at least in part, by apoptotic cell death of granulosa cells and endocrine cells of the theca interna layer. Histological, cytological, and biochemical examination of the degenerating changes cumulus cells during Follicular Atresia in porcine ovaries have confirmed that apoptosis occurs in granulosa cells but not cumulus cells or oocytes in tertiary follicles in the early stage of Atresia.
-
role of cell death ligand receptor system of granulosa cells in selective Follicular Atresia in porcine ovary
Journal of Reproduction and Development, 2011Co-Authors: Naoko Inoue, Fuko Matsuda, Yasufumi Goto, Noboru ManabeAbstract:In the mammalian ovary, more than 99% of follicles degenerate without ovulation and few oocytes ovulate and succeed to the next generation. Granulosa cell apoptosis plays a critical role in this process, Follicular Atresia. However, the molecular mechanisms responsible for the regulation of granulosa cell apoptosis have not been clarified. Death ligand and receptor systems are major apoptosis-inducing factors. This review describes the granulosa cell apoptosis via death ligand and receptor systems during Follicular Atresia in the porcine ovary.
-
bid and bax are involved in granulosa cell apoptosis during Follicular Atresia in porcine ovaries
Journal of Reproduction and Development, 2011Co-Authors: Yasufumi Goto, Fuko Matsuda, Miki Sugimoto, Akihisa Maeda, Ryousuke Yoshioka, Kannika Wongpanit, L I Junyou, Noboru ManabeAbstract:More than 99% of follicles undergo "Atresia" during Follicular development and growth. Follicular Atresia is predominantly regulated by granulosa cell apoptosis. However, the intracellular signaling pathway of apoptosis in granulosa cells has not been revealed. In the present study, we examined changes in the expression of BH3-interacting domain death agonist (Bid) and Bcl-2-associated X protein (Bax), which are considered to promote the cell death ligand/receptor-mediated process in mitochondrion-dependent type II apoptosis, in porcine granulosa cells during Atresia. Levels of mRNA and protein of Bid and Bax were determined by the reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting techniques, respectively. Levels of Bid and Bax mRNA and protein were markedly increased in granulosa cells of early atretic follicles compared with those of healthy follicles. In situ hybridization and immunohistochemical staining revealed that mRNA and protein of Bid and Bax were present in the granulosa cells, though only traces were found in healthy follicles; however, strong staining was noted in atretic follicles. These results indicate that Bid and Bax appear to be signal transduction factors in granulosa cells during Follicular Atresia and appear to play proapoptotic roles and confirm that the porcine granulosa cell is a mitochondrion-dependent type II apoptotic cell.
-
expression and function of apoptosis initiator foxo3 in granulosa cells during Follicular Atresia in pig ovaries
Journal of Reproduction and Development, 2011Co-Authors: Fuko Matsuda, Kazuhiro Sakamaki, Naoko Inoue, Yasufumi Goto, Akihisa Maeda, Yuan Cheng, Hiroshi Gonda, Noboru ManabeAbstract:In mammalian ovaries, most follicles are lost by Atresia before ovulation. It has become apparent that the apoptosis of granulosa cells induces Follicular Atresia. Forkhead box O3 (FOXO3), also called FKHRL1 (forkhead in rhabdomyosarcoma-like 1), is a proapoptotic molecule that belongs to the FOXO subfamily of forkhead transcription factors. Foxo3-deficient female mice were reported to be infertile because of abnormal ovarian Follicular development, but the precise influences of FOXO3 on Follicular Atresia of mature ovary have not been determined. Therefore, we examined the expression and function of FOXO3 in porcine ovarian follicles and granulosa-derived cells. FOXO3 mRNA levels in granulosa cells of porcine ovaries increased during Atresia, while FOXO3 protein was abundant in granulosa cells of early atretic follicles. By immunohistochemistry, the inner surface area of the granulosa layer in early atretic follicles was strongly stained with anti-FOXO3 antibody. The granulosa cells expressing FOXO3 coincided with apoptotic cells, indicating a role of FOXO3 as a proapoptotic factor in granulosa cells of porcine ovaries. In porcine (JC-410) and human (KGN) granulosa-derived cells, cell death was induced by transfection of FOXO3 expression vectors. Expression of the proapoptotic factors Fas ligand (FASLG) and BCL2-like 11 (BCL2L11) was upregulated by FOXO3 in KGN cells. In conclusion, FOXO3 is expressed in porcine ovarian follicles and induces apoptosis in granulosa cells, suggesting that it is a candidate for the initiator of Follicular Atresia.
Raymond J Rodgers - One of the best experts on this subject based on the ideXlab platform.
-
theca interna the other side of bovine Follicular Atresia
Biology of Reproduction, 2004Co-Authors: Leigh J Clark, Helen F Irvingrodgers, Arun Dharmarajan, Raymond J RodgersAbstract:Currently, histological classifications of ovarian Follicular Atresia are almost exclusively based on the morphology of the membrana granulosa without reference to the theca interna. Atresia in the bovine small antral ovarian follicle has been redefined into antral or basal Atresia where cell death commences initially within antral or basal regions of the membrana granulosa, respectively. To examine cell death in the theca interna in the two types of atretic follicles, bovine ovaries were collected and processed for immunohistochemistry and light microscopy. Follicles were classified as healthy, antral atretic, or basal atretic. Follicle diameter was recorded and sections stained with lectin from Bandeiraea simplicifolia to identify endothelial cells or with an antibody to cytochrome P450 cholesterol side-chain cleavage to identify steroidogenic cells and combined with TUNEL labeling to identify dead cells. The numerical density of steroidogenic cells within the theca interna was significantly reduced (P , 0.001) in basal atretic follicles in comparison with other follicles. Cell death was greater in both endothelial cells (P , 0.05) and steroidogenic cells (P , 0.01) of the theca interna of basal atretic follicles compared with healthy and antral atretic follicles. Thus, we conclude that the theca interna is susceptible to cell death early in Atresia, particularly in basal atretic follicles. apoptosis, Atresia, bovine, follicle, ovary, theca cells, theca interna
Shin Yonehara - One of the best experts on this subject based on the ideXlab platform.
-
involvement of fas antigen in ovarian Follicular Atresia and luteolysis
Molecular Reproduction and Development, 1997Co-Authors: Kazuhiro Sakamaki, Noboru Manabe, Hisahiro Yoshida, Yoshiko Nishimura, Shinichi Nishikawa, Shin YoneharaAbstract:The Fas antigen (Fas) is a cell-surface receptor protein that mediates apoptosis-inducing signals and plays an important role in the immune system. Significant amounts of Fas mRNA can be detected not only in lymphoid organs but also in the liver, heart, and ovary. In the ovary, apoptosis is thought to cause Follicular Atresia and luteolysis. We have investigated the involvement of Fas in these events. Here we report that Fas protein is expressed on granulosa and luteal cells but not on oocytes in the ovary. An injection of anti-Fas monoclonal antibody with apoptosis-inducing activity into adult mice enhanced Follicular Atresia and luteolysis. After the injection, the corpora lutea disappeared and the number of follicles containing pyknotic granulosa cells increased. There were also fewer ovulated ova and lower levels of luteal cell-produced progesterone. Furthermore, as the result of a non-functional Fas/Fas ligand system, mature ovaries from the mouse mutant lpr (lymphoproliferation) were histologically abnormal in terms of Follicular development, in that the number of secondary follicles significantly increased. These results suggested that Fas plays an important role in Follicular Atresia and luteolysis in the ovarian physiology of adult mice. Mol. Reprod. Dev. 47:11–18, 1997. © 1997 Wiley-Liss, Inc.