The Experts below are selected from a list of 21435 Experts worldwide ranked by ideXlab platform
Patricia M Griffin - One of the best experts on this subject based on the ideXlab platform.
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laboratory investigation of a multistate food borne Outbreak of escherichia coli o157 h7 by using pulsed field gel electrophoresis and phage typing
Journal of Clinical Microbiology, 1994Co-Authors: T J Barrett, J G Wells, B P Bell, J H Green, R Khakhria, K D Greene, J Lewis, Patricia M GriffinAbstract:Two hundred thirty-three isolates of Escherichia coli O157:H7 were analyzed by both pulsed-field gel electrophoresis (PFGE) and bacteriophage typing. All 26 isolates from persons whose illness was associated with a recent multistate Outbreak of E. coli O157:H7 infections linked to the consumption of undercooked hamburgers and all 27 isolates from incriminated lots of hamburger meat had the same phage type and the same PFGE pattern. Twenty-five of 74 E. coli O157:H7 isolates from Washington State and 10 of 27 isolates from other states obtained during the 6 months before the Outbreak had the same phage type as the Outbreak strain, but only 1 isolate had the same PFGE pattern. PFGE thus appeared to be a more sensitive method than bacteriophage typing for distinguishing Outbreak and non-Outbreak-related strains. The PFGE patterns of seven preOutbreak sporadic isolates and five sporadic isolates from the Outbreak period differed from that of the Outbreak strain by a single band, making it difficult to identify these isolates as Outbreak or non-Outbreak related. Phage typing and PFGE with additional enzymes were helpful in resolving this problem. While not as sensitive as PFGE, phage typing was helpful in interpreting PFGE data and could have been used as a simple, rapid screen to eliminate the need for performing PFGE on unrelated isolates. Images
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laboratory investigation of a multistate food borne Outbreak of escherichia coli o157 h7 by using pulsed field gel electrophoresis and phage typing
Journal of Clinical Microbiology, 1994Co-Authors: T J Barrett, J G Wells, B P Bell, J H Green, R Khakhria, K D Greene, J Lewis, Patricia M GriffinAbstract:Two hundred thirty-three isolates of Escherichia coli O157:H7 were analyzed by both pulsed-field gel electrophoresis (PFGE) and bacteriophage typing. All 26 isolates from persons whose illness was associated with a recent multistate Outbreak of E. coli O157:H7 infections linked to the consumption of undercooked hamburgers and all 27 isolates from incriminated lots of hamburger meat had the same phage type and the same PFGE pattern. Twenty-five of 74 E. coli O157:H7 isolates from Washington State and 10 of 27 isolates from other states obtained during the 6 months before the Outbreak had the same phage type as the Outbreak strain, but only 1 isolate had the same PFGE pattern. PFGE thus appeared to be a more sensitive method than bacteriophage typing for distinguishing Outbreak and non-Outbreak-related strains. The PFGE patterns of seven preOutbreak sporadic isolates and five sporadic isolates from the Outbreak period differed from that of the Outbreak strain by a single band, making it difficult to identify these isolates as Outbreak or non-Outbreak related. Phage typing and PFGE with additional enzymes were helpful in resolving this problem. While not as sensitive as PFGE, phage typing was helpful in interpreting PFGE data and could have been used as a simple, rapid screen to eliminate the need for performing PFGE on unrelated isolates.
J L Bryant - One of the best experts on this subject based on the ideXlab platform.
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Use of pulsed-field gel electrophoresis for epidemiological study of Escherichia coli O157:H7 during a Food-Borne Outbreak.
Applied and environmental microbiology, 1995Co-Authors: Janelle M. Johnson, Stephen D Weagant, Karen C Jinneman, J L BryantAbstract:Food and patient isolates from an Escherichia coli O157:H7 Outbreak associated with undercooked ground beef were characterized by pulsed-field gel electrophoresis and Shiga-like toxin genotype. Pulsed-field gel electrophoresis confirmed the epidemiologically implicated source of the two-state Outbreak and differentiated between Outbreak and sporadic strains.
Roger I. Glass - One of the best experts on this subject based on the ideXlab platform.
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characterization of a variant strain of norwalk virus from a food borne Outbreak of gastroenteritis on a cruise ship in hawaii
Journal of Clinical Microbiology, 1994Co-Authors: Barbara L Herwaldt, C D Humphrey, Stephan S. Monroe, David Lewis, Roger I. GlassAbstract:A gastroenteritis Outbreak affecting at least 217 (41%) of 527 passengers on a cruise ship was caused by a variant strain of Norwalk virus (NV) that is related to but distinct from the prototype NV strain. Consumption of fresh-cut fruit served at two buffets was significantly associated with illness (P Images
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characterization of a variant strain of norwalk virus from a food borne Outbreak of gastroenteritis on a cruise ship in hawaii
Journal of Clinical Microbiology, 1994Co-Authors: Barbara L Herwaldt, C D Humphrey, Stephan S. Monroe, D C Lewis, Roger I. GlassAbstract:A gastroenteritis Outbreak affecting at least 217 (41%) of 527 passengers on a cruise ship was caused by a variant strain of Norwalk virus (NV) that is related to but distinct from the prototype NV strain. Consumption of fresh-cut fruit served at two buffets was significantly associated with illness (P < or = 0.01), and a significant dose-response relationship was evident between illness and the number of various fresh-cut fruit items eaten. Seven (58%) of 12 paired serum specimens from ill persons demonstrated at least fourfold rises in antibody response to recombinant NV capsid antigen. A 32-nm small round-structured virus was visualized by electron microscopy in 4 (29%) of 14 fecal specimens, but none of the 8 specimens that were examined by an enzyme immunoassay for NV antigen demonstrated antigen. Four (40%) of 10 fecal specimens were positive by reverse transcriptase-PCR by using primer pairs selected from the polymerase region of NV. In a 145-bp region, the PCR product shared only 72% nucleotide sequence identity with the reference NV strain and 77% nucleotide sequence identity with Southampton virus but shared 95% nucleotide sequence identity with UK2 virus, a United Kingdom reference virus strain. In addition, the Outbreak virus was serotyped as UK2 virus by solid-phase immune electron microscopy. The genetic and antigenic divergence of the Outbreak strain from the reference NV strain highlights the need for more broadly reactive diagnostic assays and for improved understanding of the relatedness of the NV group of agents.
T J Barrett - One of the best experts on this subject based on the ideXlab platform.
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laboratory investigation of a multistate food borne Outbreak of escherichia coli o157 h7 by using pulsed field gel electrophoresis and phage typing
Journal of Clinical Microbiology, 1994Co-Authors: T J Barrett, J G Wells, B P Bell, J H Green, R Khakhria, K D Greene, J Lewis, Patricia M GriffinAbstract:Two hundred thirty-three isolates of Escherichia coli O157:H7 were analyzed by both pulsed-field gel electrophoresis (PFGE) and bacteriophage typing. All 26 isolates from persons whose illness was associated with a recent multistate Outbreak of E. coli O157:H7 infections linked to the consumption of undercooked hamburgers and all 27 isolates from incriminated lots of hamburger meat had the same phage type and the same PFGE pattern. Twenty-five of 74 E. coli O157:H7 isolates from Washington State and 10 of 27 isolates from other states obtained during the 6 months before the Outbreak had the same phage type as the Outbreak strain, but only 1 isolate had the same PFGE pattern. PFGE thus appeared to be a more sensitive method than bacteriophage typing for distinguishing Outbreak and non-Outbreak-related strains. The PFGE patterns of seven preOutbreak sporadic isolates and five sporadic isolates from the Outbreak period differed from that of the Outbreak strain by a single band, making it difficult to identify these isolates as Outbreak or non-Outbreak related. Phage typing and PFGE with additional enzymes were helpful in resolving this problem. While not as sensitive as PFGE, phage typing was helpful in interpreting PFGE data and could have been used as a simple, rapid screen to eliminate the need for performing PFGE on unrelated isolates. Images
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laboratory investigation of a multistate food borne Outbreak of escherichia coli o157 h7 by using pulsed field gel electrophoresis and phage typing
Journal of Clinical Microbiology, 1994Co-Authors: T J Barrett, J G Wells, B P Bell, J H Green, R Khakhria, K D Greene, J Lewis, Patricia M GriffinAbstract:Two hundred thirty-three isolates of Escherichia coli O157:H7 were analyzed by both pulsed-field gel electrophoresis (PFGE) and bacteriophage typing. All 26 isolates from persons whose illness was associated with a recent multistate Outbreak of E. coli O157:H7 infections linked to the consumption of undercooked hamburgers and all 27 isolates from incriminated lots of hamburger meat had the same phage type and the same PFGE pattern. Twenty-five of 74 E. coli O157:H7 isolates from Washington State and 10 of 27 isolates from other states obtained during the 6 months before the Outbreak had the same phage type as the Outbreak strain, but only 1 isolate had the same PFGE pattern. PFGE thus appeared to be a more sensitive method than bacteriophage typing for distinguishing Outbreak and non-Outbreak-related strains. The PFGE patterns of seven preOutbreak sporadic isolates and five sporadic isolates from the Outbreak period differed from that of the Outbreak strain by a single band, making it difficult to identify these isolates as Outbreak or non-Outbreak related. Phage typing and PFGE with additional enzymes were helpful in resolving this problem. While not as sensitive as PFGE, phage typing was helpful in interpreting PFGE data and could have been used as a simple, rapid screen to eliminate the need for performing PFGE on unrelated isolates.
J H Green - One of the best experts on this subject based on the ideXlab platform.
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laboratory investigation of a multistate food borne Outbreak of escherichia coli o157 h7 by using pulsed field gel electrophoresis and phage typing
Journal of Clinical Microbiology, 1994Co-Authors: T J Barrett, J G Wells, B P Bell, J H Green, R Khakhria, K D Greene, J Lewis, Patricia M GriffinAbstract:Two hundred thirty-three isolates of Escherichia coli O157:H7 were analyzed by both pulsed-field gel electrophoresis (PFGE) and bacteriophage typing. All 26 isolates from persons whose illness was associated with a recent multistate Outbreak of E. coli O157:H7 infections linked to the consumption of undercooked hamburgers and all 27 isolates from incriminated lots of hamburger meat had the same phage type and the same PFGE pattern. Twenty-five of 74 E. coli O157:H7 isolates from Washington State and 10 of 27 isolates from other states obtained during the 6 months before the Outbreak had the same phage type as the Outbreak strain, but only 1 isolate had the same PFGE pattern. PFGE thus appeared to be a more sensitive method than bacteriophage typing for distinguishing Outbreak and non-Outbreak-related strains. The PFGE patterns of seven preOutbreak sporadic isolates and five sporadic isolates from the Outbreak period differed from that of the Outbreak strain by a single band, making it difficult to identify these isolates as Outbreak or non-Outbreak related. Phage typing and PFGE with additional enzymes were helpful in resolving this problem. While not as sensitive as PFGE, phage typing was helpful in interpreting PFGE data and could have been used as a simple, rapid screen to eliminate the need for performing PFGE on unrelated isolates. Images
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laboratory investigation of a multistate food borne Outbreak of escherichia coli o157 h7 by using pulsed field gel electrophoresis and phage typing
Journal of Clinical Microbiology, 1994Co-Authors: T J Barrett, J G Wells, B P Bell, J H Green, R Khakhria, K D Greene, J Lewis, Patricia M GriffinAbstract:Two hundred thirty-three isolates of Escherichia coli O157:H7 were analyzed by both pulsed-field gel electrophoresis (PFGE) and bacteriophage typing. All 26 isolates from persons whose illness was associated with a recent multistate Outbreak of E. coli O157:H7 infections linked to the consumption of undercooked hamburgers and all 27 isolates from incriminated lots of hamburger meat had the same phage type and the same PFGE pattern. Twenty-five of 74 E. coli O157:H7 isolates from Washington State and 10 of 27 isolates from other states obtained during the 6 months before the Outbreak had the same phage type as the Outbreak strain, but only 1 isolate had the same PFGE pattern. PFGE thus appeared to be a more sensitive method than bacteriophage typing for distinguishing Outbreak and non-Outbreak-related strains. The PFGE patterns of seven preOutbreak sporadic isolates and five sporadic isolates from the Outbreak period differed from that of the Outbreak strain by a single band, making it difficult to identify these isolates as Outbreak or non-Outbreak related. Phage typing and PFGE with additional enzymes were helpful in resolving this problem. While not as sensitive as PFGE, phage typing was helpful in interpreting PFGE data and could have been used as a simple, rapid screen to eliminate the need for performing PFGE on unrelated isolates.