The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Ekaterina P Khristunova - One of the best experts on this subject based on the ideXlab platform.
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simultaneous voltammetric determination of brilliant blue fcf and tartrazine for Food Quality Control
Talanta, 2020Co-Authors: Olga I Lipskikh, Ekaterina P Khristunova, E. I. Korotkova, Jiri Barek, Vlastimil Vyskocil, Muhammad SaqibAbstract:Abstract Voltammetric determination of Tartrazine (Tz) and Brilliant Blue FCF (BB) in their mixture using novel type of carbon black-polyethylene composite electrode (CBPCE) with renewable surface modified by carbon ink (CI) was developed. Electrochemical properties of the tested dyes were investigated in 0.1 mol L−1 Britton-Robinson (BR) buffer by cyclic voltammetry (CV) and linear scan voltammetry (LSV). Simultaneous determination of the dyes is based on the application of supporting electrolytes with different pH: 2.0 for Tz and 10.0 for BB. Under the optimum experimental conditions, linear concentration dependences in the concentration ranges from 0.037 to 1.38 μmol L−1 for Tz and from 0.025 to 2.52 μmol L−1 for BB were obtained by LSV in the first-order derivative mode. Limits of detection (LODs) for Tz and BB were 0.019 and 0.011 μmol L−1, respectively. The modified electrode showed good stability and reproducibility and was successfully applied for the determination of the mixture Tz and BB in a candy and soft drink products.
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simultaneous voltammetric determination of brilliant blue fcf and tartrazine for Food Quality Control
Talanta, 2020Co-Authors: Olga I Lipskikh, Ekaterina P Khristunova, E. I. Korotkova, Jiri Barek, Vlastimil Vyskocil, Muhammad SaqibAbstract:Abstract Voltammetric determination of Tartrazine (Tz) and Brilliant Blue FCF (BB) in their mixture using novel type of carbon black-polyethylene composite electrode (CBPCE) with renewable surface modified by carbon ink (CI) was developed. Electrochemical properties of the tested dyes were investigated in 0.1 mol L−1 Britton-Robinson (BR) buffer by cyclic voltammetry (CV) and linear scan voltammetry (LSV). Simultaneous determination of the dyes is based on the application of supporting electrolytes with different pH: 2.0 for Tz and 10.0 for BB. Under the optimum experimental conditions, linear concentration dependences in the concentration ranges from 0.037 to 1.38 μmol L−1 for Tz and from 0.025 to 2.52 μmol L−1 for BB were obtained by LSV in the first-order derivative mode. Limits of detection (LODs) for Tz and BB were 0.019 and 0.011 μmol L−1, respectively. The modified electrode showed good stability and reproducibility and was successfully applied for the determination of the mixture Tz and BB in a candy and soft drink products.
Otacilio C Moreira - One of the best experts on this subject based on the ideXlab platform.
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validation of a novel multiplex real time pcr assay for trypanosoma cruzi detection and quantification in acai pulp
PLOS ONE, 2021Co-Authors: Paula Finamorearaujo, Amanda Faierpereira, Carlos Ramon Do Nascimento Brito, Eldrinei Gomes Peres, Klenicy Kazumy De Lima Yamaguchi, Renata Trotta Barroso Ferreira, Otacilio C MoreiraAbstract:In Brazil, orally acquired T. cruzi infection has become the most relevant transmission mechanisms from public health perspective. Around 70% of new Chagas disease cases have been associated with consumption of contaminated Food or beverages. Acai (Euterpe oleracea and Euterpe precatoria) is currently one of the most commercialized Amazonian fruits in the Brazilian and international markets. Therefore, it has become important to incorporate in the production process some procedures to measure out effective hygiene and product Quality Control required by global market. Molecular methods have been developed for rapid detection and quantification of T. cruzi DNA in several biological samples, including Food matrices, for epidemiological investigation of Chagas disease and Food Quality Control. However, a high-performance molecular methodology since DNA extraction until detection and quantification of T. cruzi DNA in acai berry pulp is still needed. Herein, a simple DNA extraction methodology was standardized from the supernatant of acai berry pulp stabilized in a 6M Guanidine-HCl/0.2M EDTA buffer. In addition, a multiplex real time qPCR assay, targeting T. cruzi DNA and an Exogenous Internal Positive Control was developed and validated, using reference from all T. cruzi DTUs and commercial samples of acai pulp, from an endemic municipality with previous history of oral Chagas disease outbreak. Thus, a high-sensitivity qPCR assay, that could detect up to 0.01 parasite equivalents/mL in acai, was reached. As of the 45 commercial samples analyzed, 9 (20%) were positive for T. cruzi. This high-sensitive, fast, and easy-to-use molecular assay is compatible with most of the laboratories involved in the investigations of oral Chagas disease outbreaks, representing an important tool to the epidemiology, Control, and surveillance of Chagas disease.
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validation of a novel multiplex real time pcr assay for trypanosoma cruzi detection and quantification in acai pulp
bioRxiv, 2020Co-Authors: Paula Finamorearaujo, Amanda Faierpereira, Carlos Ramon Do Nascimento Brito, Eldrinei Gomes Peres, Klenicy Kazumy De Lima Yamaguchi, Renata Trotta Barroso Ferreira, Otacilio C MoreiraAbstract:In Brazil, orally acquired T. cruzi infection has become the most relevant transmission mechanisms from public health perspective. Around 70% of new Chagas disease cases have been associated with consumption of contaminated Food or beverages. Acai (Euterpe oleracea and Euterpe precatoria) is currently one of the most commercialized Amazonian fruits in the Brazilian and international markets. Therefore, it has become important to incorporate in the production process some procedures to measure out effective hygiene and product Quality Control required by global market. Molecular methods have been developed for rapid detection and quantification of T. cruzi DNA in several biological samples, including Food matrices, for epidemiological investigation of Chagas disease and Food Quality Control. However, a high-performance molecular methodology since DNA extraction until detection and quantification of T. cruzi DNA in acai berry pulp is still needed. Herein, a simple DNA extraction methodology was standardized from the supernatant of acai berry pulp stabilized in a Lysis buffer. In addition, a multiplex real time qPCR assay, targeting T. cruzi DNA and an Exogenous Internal Positive Control was developed and validated, using reference from all T. cruzi DTUs and commercial samples of acai pulp, from an endemic municipality with previous history of oral Chagas disease outbreak. Thus, a high-sensitivity qPCR assay, that could detect up to 0.01 parasite equivalents/mL in acai, was reached. As of the 45 commercial samples analyzed, 9 (20%) were positive for T. cruzi. This high-sensitive, fast and easy-to-use molecular assay is compatible with most of the laboratories involved in the investigations of oral Chagas disease outbreaks, representing an important tool to the epidemiology, Control and surveillance of Chagas disease.
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Detection and genotyping of Trypanosoma cruzi from açai products commercialized in Rio de Janeiro and Pará, Brazil
Parasites & Vectors, 2018Co-Authors: Renata Trotta Barroso Ferreira, Maria Luiza Cabral, Ronald Sodré Martins, Paula Finamore Araujo, Sérgio Alves Da Silva, Constança Britto, Maria Regina Branquinho, Paola Cardarelli-leite, Otacilio C MoreiraAbstract:Background Several cases of Food-borne acute Chagas disease (ACD) have been reported in the Brazilian Amazon so far. Up to 2004, the occurrence of ACD by oral transmission, associated with Food consumption, was rare. Recent cases of ACD in Brazil have been attributed to the consumption of juice from the açai palm containing reservoir animals or insect vectors waste, infected with Trypanosoma cruzi . This study aimed to determine the T. cruzi contamination rate and to genotype the parasite in Food samples prepared from açai, which are commercialized in Rio de Janeiro and the Pará States in Brazil. Methods The amplificability of DNA extracted from açai samples, and T. cruzi and Triatominae detection were performed by conventional PCR. Molecular characterization was done by multilocus PCR analysis, to determine the parasite discrete type units (DTUs) based on the size of PCR products in agarose gels, using the intergenic region of the spliced leader (SL), 24 Sα rDNA and nuclear fragment A10 as targets. Results From the 140 samples of açai-based products analyzed, T. cruzi DNA was detected in 14 samples (10%); triatomine DNA was detected in one of these 14 samples. The parasite genotyping demonstrated that Food samples containing açai showed a mixture of T. cruzi DTUs with TcIII, TcV and TcI prevailing. Conclusions In this study, the molecular detection and identification of T. cruzi from açai-based manufactured Food samples, was performed for the first time. Although parasite DNA is a marker of possible contamination during Food manufacturing, our findings do not indicate that açai is a source of Chagas disease via oral transmission per se, as live parasites were not investigated. Nevertheless, a molecular approach could be a powerful tool in the epidemiological investigation of outbreaks, supporting previous evidence that açai-based Food can be contaminated with T. cruzi . Furthermore, both Food Quality Control and assessment of good manufacturing practices involving açai-based products can be improved, assuring the safety of açai products.
Renata Trotta Barroso Ferreira - One of the best experts on this subject based on the ideXlab platform.
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validation of a novel multiplex real time pcr assay for trypanosoma cruzi detection and quantification in acai pulp
PLOS ONE, 2021Co-Authors: Paula Finamorearaujo, Amanda Faierpereira, Carlos Ramon Do Nascimento Brito, Eldrinei Gomes Peres, Klenicy Kazumy De Lima Yamaguchi, Renata Trotta Barroso Ferreira, Otacilio C MoreiraAbstract:In Brazil, orally acquired T. cruzi infection has become the most relevant transmission mechanisms from public health perspective. Around 70% of new Chagas disease cases have been associated with consumption of contaminated Food or beverages. Acai (Euterpe oleracea and Euterpe precatoria) is currently one of the most commercialized Amazonian fruits in the Brazilian and international markets. Therefore, it has become important to incorporate in the production process some procedures to measure out effective hygiene and product Quality Control required by global market. Molecular methods have been developed for rapid detection and quantification of T. cruzi DNA in several biological samples, including Food matrices, for epidemiological investigation of Chagas disease and Food Quality Control. However, a high-performance molecular methodology since DNA extraction until detection and quantification of T. cruzi DNA in acai berry pulp is still needed. Herein, a simple DNA extraction methodology was standardized from the supernatant of acai berry pulp stabilized in a 6M Guanidine-HCl/0.2M EDTA buffer. In addition, a multiplex real time qPCR assay, targeting T. cruzi DNA and an Exogenous Internal Positive Control was developed and validated, using reference from all T. cruzi DTUs and commercial samples of acai pulp, from an endemic municipality with previous history of oral Chagas disease outbreak. Thus, a high-sensitivity qPCR assay, that could detect up to 0.01 parasite equivalents/mL in acai, was reached. As of the 45 commercial samples analyzed, 9 (20%) were positive for T. cruzi. This high-sensitive, fast, and easy-to-use molecular assay is compatible with most of the laboratories involved in the investigations of oral Chagas disease outbreaks, representing an important tool to the epidemiology, Control, and surveillance of Chagas disease.
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validation of a novel multiplex real time pcr assay for trypanosoma cruzi detection and quantification in acai pulp
bioRxiv, 2020Co-Authors: Paula Finamorearaujo, Amanda Faierpereira, Carlos Ramon Do Nascimento Brito, Eldrinei Gomes Peres, Klenicy Kazumy De Lima Yamaguchi, Renata Trotta Barroso Ferreira, Otacilio C MoreiraAbstract:In Brazil, orally acquired T. cruzi infection has become the most relevant transmission mechanisms from public health perspective. Around 70% of new Chagas disease cases have been associated with consumption of contaminated Food or beverages. Acai (Euterpe oleracea and Euterpe precatoria) is currently one of the most commercialized Amazonian fruits in the Brazilian and international markets. Therefore, it has become important to incorporate in the production process some procedures to measure out effective hygiene and product Quality Control required by global market. Molecular methods have been developed for rapid detection and quantification of T. cruzi DNA in several biological samples, including Food matrices, for epidemiological investigation of Chagas disease and Food Quality Control. However, a high-performance molecular methodology since DNA extraction until detection and quantification of T. cruzi DNA in acai berry pulp is still needed. Herein, a simple DNA extraction methodology was standardized from the supernatant of acai berry pulp stabilized in a Lysis buffer. In addition, a multiplex real time qPCR assay, targeting T. cruzi DNA and an Exogenous Internal Positive Control was developed and validated, using reference from all T. cruzi DTUs and commercial samples of acai pulp, from an endemic municipality with previous history of oral Chagas disease outbreak. Thus, a high-sensitivity qPCR assay, that could detect up to 0.01 parasite equivalents/mL in acai, was reached. As of the 45 commercial samples analyzed, 9 (20%) were positive for T. cruzi. This high-sensitive, fast and easy-to-use molecular assay is compatible with most of the laboratories involved in the investigations of oral Chagas disease outbreaks, representing an important tool to the epidemiology, Control and surveillance of Chagas disease.
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Detection and genotyping of Trypanosoma cruzi from açai products commercialized in Rio de Janeiro and Pará, Brazil
Parasites & Vectors, 2018Co-Authors: Renata Trotta Barroso Ferreira, Maria Luiza Cabral, Ronald Sodré Martins, Paula Finamore Araujo, Sérgio Alves Da Silva, Constança Britto, Maria Regina Branquinho, Paola Cardarelli-leite, Otacilio C MoreiraAbstract:Background Several cases of Food-borne acute Chagas disease (ACD) have been reported in the Brazilian Amazon so far. Up to 2004, the occurrence of ACD by oral transmission, associated with Food consumption, was rare. Recent cases of ACD in Brazil have been attributed to the consumption of juice from the açai palm containing reservoir animals or insect vectors waste, infected with Trypanosoma cruzi . This study aimed to determine the T. cruzi contamination rate and to genotype the parasite in Food samples prepared from açai, which are commercialized in Rio de Janeiro and the Pará States in Brazil. Methods The amplificability of DNA extracted from açai samples, and T. cruzi and Triatominae detection were performed by conventional PCR. Molecular characterization was done by multilocus PCR analysis, to determine the parasite discrete type units (DTUs) based on the size of PCR products in agarose gels, using the intergenic region of the spliced leader (SL), 24 Sα rDNA and nuclear fragment A10 as targets. Results From the 140 samples of açai-based products analyzed, T. cruzi DNA was detected in 14 samples (10%); triatomine DNA was detected in one of these 14 samples. The parasite genotyping demonstrated that Food samples containing açai showed a mixture of T. cruzi DTUs with TcIII, TcV and TcI prevailing. Conclusions In this study, the molecular detection and identification of T. cruzi from açai-based manufactured Food samples, was performed for the first time. Although parasite DNA is a marker of possible contamination during Food manufacturing, our findings do not indicate that açai is a source of Chagas disease via oral transmission per se, as live parasites were not investigated. Nevertheless, a molecular approach could be a powerful tool in the epidemiological investigation of outbreaks, supporting previous evidence that açai-based Food can be contaminated with T. cruzi . Furthermore, both Food Quality Control and assessment of good manufacturing practices involving açai-based products can be improved, assuring the safety of açai products.
Kentye Yong - One of the best experts on this subject based on the ideXlab platform.
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nanomaterials enhanced surface plasmon resonance for biological and chemical sensing applications
Chemical Society Reviews, 2014Co-Authors: Shuwen Zeng, Dominique Baillargeat, Kentye YongAbstract:The main challenge for all electrical, mechanical and optical sensors is to detect low molecular weight (less than 400 Da) chemical and biological analytes under extremely dilute conditions. Surface plasmon resonance sensors are the most commonly used optical sensors due to their unique ability for real-time monitoring the molecular binding events. However, their sensitivities are insufficient to detect trace amounts of small molecular weight molecules such as cancer biomarkers, hormones, antibiotics, insecticides, and explosive materials which are respectively important for early-stage disease diagnosis, Food Quality Control, environmental monitoring, and homeland security protection. With the rapid development of nanotechnology in the past few years, nanomaterials-enhanced surface plasmon resonance sensors have been developed and used as effective tools to sense hard-to-detect molecules within the concentration range between pmol and amol. In this review article, we reviewed and discussed the latest trend and challenges in engineering and applications of nanomaterials-enhanced surface plasmon resonance sensors (e.g., metallic nanoparticles, magnetic nanoparticles, carbon-based nanomaterials, latex nanoparticles and liposome nanoparticles) for detecting “hard-to-identify” biological and chemical analytes. Such information will be viable in terms of providing a useful platform for designing future ultrasensitive plasmonic nanosensors.
Stella Vallejos - One of the best experts on this subject based on the ideXlab platform.
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love wave sensors with silver modified polypyrrole nanoparticles for vocs monitoring
Sensors, 2020Co-Authors: Milena Setka, D. Matatagui, F A Bahos, I Gracia, Eduard Figueras, Jana Drbohlavova, Stella VallejosAbstract:Love wave sensors with silver-modified polypyrrole nanoparticles are developed in this work. These systems prove functional at room temperature with enhanced response, sensitivity and response time, as compared to other state-of-the-art surface acoustic wave (SAW) sensors, towards volatile organic compounds (VOCs). Results demonstrate the monitoring of hundreds of ppb of compounds such as acetone, ethanol and toluene with low estimated limits of detection (~3 ppb for acetone). These results are attributed to the use of silver-modified polypyrrole as a second guiding/sensitive layer in the Love wave sensor structure, which provides further chemically active sites for the gas-solid interactions. The sensing of low VOCs concentrations by micro sensing elements as those presented here could be beneficial in future systems for air Quality Control, Food Quality Control or disease diagnosis via exhaled breath as the limits of detection obtained are within those required in these applications.