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Abdennaceur Hassen - One of the best experts on this subject based on the ideXlab platform.
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Inactivation of HepatoVirus A in wastewater by 254 nm ultraviolet-C irradiation
Environmental Science and Pollution Research, 2020Co-Authors: Chourouk Ibrahim, Rawand Hamdi, Salah Hammami, Pierre Pothier, Nesserine Khelifi, Abdennaceur HassenAbstract:HepatoVirus A is known as a waterborne and Foodborne Virus that can be transmitted from one person to another through contaminated water and raw food. Therefore, it is necessary to survey the circulation of this type of enteric Virus in the wastewater to prevent prospective outbreaks. Wastewater samples collected from WWTP El Menzeh I and Charguia I have been the subject for physicochemical, bacteriological (MPN) and virological analyses. HepatoVirus A (HAV) detection was carried out using the standard reverse transcription-polymerase chain reaction (RT-PCR). HepatoVirus A was detected respectively in 62% (63/102) and 66% (92/140) of the collected wastewater samples at El Menzeh I and Charguia I WWTPs. The treated effluent by natural oxidizing lagoon procedure was characterized by a poor physical-chemical and virological qualities but with excellent bacteriological quality. Consequently, this effluent is not suitable to be recycled and reused in agriculture or even dismissed in the environment. The treated sewage by activated sludge and rotating biodisk procedures turned out to be of a very good physical-chemical quality but with a poor bacteriological and virological quality. After tertiary UV-C_254 nm irradiation, the faecal indicator bacteria concentration was mostly reduced and removed. These findings confirmed the need for improvement and upgrade of the treatment processes used in these two studied sewage purification plants and the necessity of implementation and establishment of a proper national virological standard to control the circulation rates of enteric Viruses in Tunisian municipal wastewater.
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inactivation of hepatoVirus a in wastewater by 254 nm ultraviolet c irradiation
Environmental Science and Pollution Research, 2020Co-Authors: Chourouk Ibrahim, Rawand Hamdi, Salah Hammami, Pierre Pothier, Nesserine Khelifi, Abdennaceur HassenAbstract:HepatoVirus A is known as a waterborne and Foodborne Virus that can be transmitted from one person to another through contaminated water and raw food. Therefore, it is necessary to survey the circulation of this type of enteric Virus in the wastewater to prevent prospective outbreaks. Wastewater samples collected from WWTP El Menzeh I and Charguia I have been the subject for physicochemical, bacteriological (MPN) and virological analyses. HepatoVirus A (HAV) detection was carried out using the standard reverse transcription-polymerase chain reaction (RT-PCR). HepatoVirus A was detected respectively in 62% (63/102) and 66% (92/140) of the collected wastewater samples at El Menzeh I and Charguia I WWTPs. The treated effluent by natural oxidizing lagoon procedure was characterized by a poor physical-chemical and virological qualities but with excellent bacteriological quality. Consequently, this effluent is not suitable to be recycled and reused in agriculture or even dismissed in the environment. The treated sewage by activated sludge and rotating biodisk procedures turned out to be of a very good physical-chemical quality but with a poor bacteriological and virological quality. After tertiary UV-C254 nm irradiation, the faecal indicator bacteria concentration was mostly reduced and removed. These findings confirmed the need for improvement and upgrade of the treatment processes used in these two studied sewage purification plants and the necessity of implementation and establishment of a proper national virological standard to control the circulation rates of enteric Viruses in Tunisian municipal wastewater.
Chourouk Ibrahim - One of the best experts on this subject based on the ideXlab platform.
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Inactivation of HepatoVirus A in wastewater by 254 nm ultraviolet-C irradiation
Environmental Science and Pollution Research, 2020Co-Authors: Chourouk Ibrahim, Rawand Hamdi, Salah Hammami, Pierre Pothier, Nesserine Khelifi, Abdennaceur HassenAbstract:HepatoVirus A is known as a waterborne and Foodborne Virus that can be transmitted from one person to another through contaminated water and raw food. Therefore, it is necessary to survey the circulation of this type of enteric Virus in the wastewater to prevent prospective outbreaks. Wastewater samples collected from WWTP El Menzeh I and Charguia I have been the subject for physicochemical, bacteriological (MPN) and virological analyses. HepatoVirus A (HAV) detection was carried out using the standard reverse transcription-polymerase chain reaction (RT-PCR). HepatoVirus A was detected respectively in 62% (63/102) and 66% (92/140) of the collected wastewater samples at El Menzeh I and Charguia I WWTPs. The treated effluent by natural oxidizing lagoon procedure was characterized by a poor physical-chemical and virological qualities but with excellent bacteriological quality. Consequently, this effluent is not suitable to be recycled and reused in agriculture or even dismissed in the environment. The treated sewage by activated sludge and rotating biodisk procedures turned out to be of a very good physical-chemical quality but with a poor bacteriological and virological quality. After tertiary UV-C_254 nm irradiation, the faecal indicator bacteria concentration was mostly reduced and removed. These findings confirmed the need for improvement and upgrade of the treatment processes used in these two studied sewage purification plants and the necessity of implementation and establishment of a proper national virological standard to control the circulation rates of enteric Viruses in Tunisian municipal wastewater.
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inactivation of hepatoVirus a in wastewater by 254 nm ultraviolet c irradiation
Environmental Science and Pollution Research, 2020Co-Authors: Chourouk Ibrahim, Rawand Hamdi, Salah Hammami, Pierre Pothier, Nesserine Khelifi, Abdennaceur HassenAbstract:HepatoVirus A is known as a waterborne and Foodborne Virus that can be transmitted from one person to another through contaminated water and raw food. Therefore, it is necessary to survey the circulation of this type of enteric Virus in the wastewater to prevent prospective outbreaks. Wastewater samples collected from WWTP El Menzeh I and Charguia I have been the subject for physicochemical, bacteriological (MPN) and virological analyses. HepatoVirus A (HAV) detection was carried out using the standard reverse transcription-polymerase chain reaction (RT-PCR). HepatoVirus A was detected respectively in 62% (63/102) and 66% (92/140) of the collected wastewater samples at El Menzeh I and Charguia I WWTPs. The treated effluent by natural oxidizing lagoon procedure was characterized by a poor physical-chemical and virological qualities but with excellent bacteriological quality. Consequently, this effluent is not suitable to be recycled and reused in agriculture or even dismissed in the environment. The treated sewage by activated sludge and rotating biodisk procedures turned out to be of a very good physical-chemical quality but with a poor bacteriological and virological quality. After tertiary UV-C254 nm irradiation, the faecal indicator bacteria concentration was mostly reduced and removed. These findings confirmed the need for improvement and upgrade of the treatment processes used in these two studied sewage purification plants and the necessity of implementation and establishment of a proper national virological standard to control the circulation rates of enteric Viruses in Tunisian municipal wastewater.
Doris H. D'souza - One of the best experts on this subject based on the ideXlab platform.
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Grape seed extract for Foodborne Virus reduction on produce.
Food microbiology, 2012Co-Authors: Doris H. D'souzaAbstract:Abstract Grape seed extract (GSE) is reported to have antibacterial properties with few current studies on antiviral activity. Recently, we reported the effects of GSE against Foodborne viral surrogates in vitro . This study evaluated the application of GSE (commercial Gravinol-S) against hepatitis A Virus (HAV) and human noroVirus surrogates, feline caliciVirus (FCV-F9) and murine noroVirus (MNV-1), on model produce. Washed and air-dried lettuce (3 × 3 cm 2 ) and jalapeno peppers (25–30 g) were inoculated with FCV-F9, MNV-1, or HAV at high (∼7 log 10 PFU/ml) or low (∼5 log 10 PFU/ml) titers, and treated with 0.25, 0.5, 1 mg/ml GSE or water for 30 s to 5 min. Treatments were stopped/diluted with cell-culture media containing 10% heat-inactivated fetal bovine serum and evaluated using plaque assays. At high titers, FCV-F9 was reduced by 2.33, 2.58, and 2.71 log 10 PFU on lettuce; and 2.20, 2.74, and 3.05 log 10 PFU on peppers after 1 min using 0.25, 0.50, and 1 mg/ml GSE, respectively. Low FCV-F9 titers could not be detected after 1 min at all three GSE concentrations. Low titer MNV-1 was reduced by 0.2–0.3 log 10 PFU on lettuce and 0.8 log 10 PFU on peppers, without reduction of high titer. GSE at 0.25–1 mg/ml after 1 min caused 0.7–1.1 and 1–1.3 log 10 PFU reduction for high and low HAV titers, respectively on both commodities. Instrumental color analysis showed no significant differences between treated and untreated produce. GSE shows potential for Foodborne viral reduction on produce as part of hurdle technologies.
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Inactivation of human enteric Virus surrogates by high-intensity ultrasound.
Foodborne Pathogens and Disease, 2010Co-Authors: Xiaowei Su, Svetlana Zivanovic, Doris H. D'souzaAbstract:Abstract Foodborne Viruses, especially human noroViruses, are recognized as leading causes of nonbacterial gastroenteritis worldwide. Development of effective inactivation methods is of great importance to control their spread. In this study, the effect of high-intensity ultrasound (HIUS) on the infectivity of three Foodborne Virus surrogates was investigated. The three surrogates, murine noroVirus (MNV-1), feline caliciVirus (FCV-F9), and MS2 bacteriophage, were diluted in phosphate-buffered saline (PBS) or orange juice to a titer of ∼6 log10 PFU/mL or ∼4 log10 PFU/mL. The ultrasound treatment was performed in duplicate by immersing the HIUS probe in Virus-containing solution that was cooled in ice-water and sonicated at 20 kHz for 2, 5, 10, 15, 20, and 30 min with 30 sec on and 30 sec off. The infectivity of the recovered Viruses after each ultrasound treatment was evaluated in duplicate using standardized plaque assays and compared to untreated controls. The results show that HIUS effectiveness depende...
Rawand Hamdi - One of the best experts on this subject based on the ideXlab platform.
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Inactivation of HepatoVirus A in wastewater by 254 nm ultraviolet-C irradiation
Environmental Science and Pollution Research, 2020Co-Authors: Chourouk Ibrahim, Rawand Hamdi, Salah Hammami, Pierre Pothier, Nesserine Khelifi, Abdennaceur HassenAbstract:HepatoVirus A is known as a waterborne and Foodborne Virus that can be transmitted from one person to another through contaminated water and raw food. Therefore, it is necessary to survey the circulation of this type of enteric Virus in the wastewater to prevent prospective outbreaks. Wastewater samples collected from WWTP El Menzeh I and Charguia I have been the subject for physicochemical, bacteriological (MPN) and virological analyses. HepatoVirus A (HAV) detection was carried out using the standard reverse transcription-polymerase chain reaction (RT-PCR). HepatoVirus A was detected respectively in 62% (63/102) and 66% (92/140) of the collected wastewater samples at El Menzeh I and Charguia I WWTPs. The treated effluent by natural oxidizing lagoon procedure was characterized by a poor physical-chemical and virological qualities but with excellent bacteriological quality. Consequently, this effluent is not suitable to be recycled and reused in agriculture or even dismissed in the environment. The treated sewage by activated sludge and rotating biodisk procedures turned out to be of a very good physical-chemical quality but with a poor bacteriological and virological quality. After tertiary UV-C_254 nm irradiation, the faecal indicator bacteria concentration was mostly reduced and removed. These findings confirmed the need for improvement and upgrade of the treatment processes used in these two studied sewage purification plants and the necessity of implementation and establishment of a proper national virological standard to control the circulation rates of enteric Viruses in Tunisian municipal wastewater.
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inactivation of hepatoVirus a in wastewater by 254 nm ultraviolet c irradiation
Environmental Science and Pollution Research, 2020Co-Authors: Chourouk Ibrahim, Rawand Hamdi, Salah Hammami, Pierre Pothier, Nesserine Khelifi, Abdennaceur HassenAbstract:HepatoVirus A is known as a waterborne and Foodborne Virus that can be transmitted from one person to another through contaminated water and raw food. Therefore, it is necessary to survey the circulation of this type of enteric Virus in the wastewater to prevent prospective outbreaks. Wastewater samples collected from WWTP El Menzeh I and Charguia I have been the subject for physicochemical, bacteriological (MPN) and virological analyses. HepatoVirus A (HAV) detection was carried out using the standard reverse transcription-polymerase chain reaction (RT-PCR). HepatoVirus A was detected respectively in 62% (63/102) and 66% (92/140) of the collected wastewater samples at El Menzeh I and Charguia I WWTPs. The treated effluent by natural oxidizing lagoon procedure was characterized by a poor physical-chemical and virological qualities but with excellent bacteriological quality. Consequently, this effluent is not suitable to be recycled and reused in agriculture or even dismissed in the environment. The treated sewage by activated sludge and rotating biodisk procedures turned out to be of a very good physical-chemical quality but with a poor bacteriological and virological quality. After tertiary UV-C254 nm irradiation, the faecal indicator bacteria concentration was mostly reduced and removed. These findings confirmed the need for improvement and upgrade of the treatment processes used in these two studied sewage purification plants and the necessity of implementation and establishment of a proper national virological standard to control the circulation rates of enteric Viruses in Tunisian municipal wastewater.
Salah Hammami - One of the best experts on this subject based on the ideXlab platform.
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Inactivation of HepatoVirus A in wastewater by 254 nm ultraviolet-C irradiation
Environmental Science and Pollution Research, 2020Co-Authors: Chourouk Ibrahim, Rawand Hamdi, Salah Hammami, Pierre Pothier, Nesserine Khelifi, Abdennaceur HassenAbstract:HepatoVirus A is known as a waterborne and Foodborne Virus that can be transmitted from one person to another through contaminated water and raw food. Therefore, it is necessary to survey the circulation of this type of enteric Virus in the wastewater to prevent prospective outbreaks. Wastewater samples collected from WWTP El Menzeh I and Charguia I have been the subject for physicochemical, bacteriological (MPN) and virological analyses. HepatoVirus A (HAV) detection was carried out using the standard reverse transcription-polymerase chain reaction (RT-PCR). HepatoVirus A was detected respectively in 62% (63/102) and 66% (92/140) of the collected wastewater samples at El Menzeh I and Charguia I WWTPs. The treated effluent by natural oxidizing lagoon procedure was characterized by a poor physical-chemical and virological qualities but with excellent bacteriological quality. Consequently, this effluent is not suitable to be recycled and reused in agriculture or even dismissed in the environment. The treated sewage by activated sludge and rotating biodisk procedures turned out to be of a very good physical-chemical quality but with a poor bacteriological and virological quality. After tertiary UV-C_254 nm irradiation, the faecal indicator bacteria concentration was mostly reduced and removed. These findings confirmed the need for improvement and upgrade of the treatment processes used in these two studied sewage purification plants and the necessity of implementation and establishment of a proper national virological standard to control the circulation rates of enteric Viruses in Tunisian municipal wastewater.
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inactivation of hepatoVirus a in wastewater by 254 nm ultraviolet c irradiation
Environmental Science and Pollution Research, 2020Co-Authors: Chourouk Ibrahim, Rawand Hamdi, Salah Hammami, Pierre Pothier, Nesserine Khelifi, Abdennaceur HassenAbstract:HepatoVirus A is known as a waterborne and Foodborne Virus that can be transmitted from one person to another through contaminated water and raw food. Therefore, it is necessary to survey the circulation of this type of enteric Virus in the wastewater to prevent prospective outbreaks. Wastewater samples collected from WWTP El Menzeh I and Charguia I have been the subject for physicochemical, bacteriological (MPN) and virological analyses. HepatoVirus A (HAV) detection was carried out using the standard reverse transcription-polymerase chain reaction (RT-PCR). HepatoVirus A was detected respectively in 62% (63/102) and 66% (92/140) of the collected wastewater samples at El Menzeh I and Charguia I WWTPs. The treated effluent by natural oxidizing lagoon procedure was characterized by a poor physical-chemical and virological qualities but with excellent bacteriological quality. Consequently, this effluent is not suitable to be recycled and reused in agriculture or even dismissed in the environment. The treated sewage by activated sludge and rotating biodisk procedures turned out to be of a very good physical-chemical quality but with a poor bacteriological and virological quality. After tertiary UV-C254 nm irradiation, the faecal indicator bacteria concentration was mostly reduced and removed. These findings confirmed the need for improvement and upgrade of the treatment processes used in these two studied sewage purification plants and the necessity of implementation and establishment of a proper national virological standard to control the circulation rates of enteric Viruses in Tunisian municipal wastewater.