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Teresa M Barbosa - One of the best experts on this subject based on the ideXlab platform.

  • diversity and bioactive potential of endospore Forming Bacteria cultured from the marine sponge haliclona simulans
    Journal of Applied Microbiology, 2012
    Co-Authors: Robert W Phelan, John Ohalloran, Jonathan Kennedy, John P Morrissey, Alan D W Dobson, Fergal Ogara, Teresa M Barbosa
    Abstract:

    Aims:  Despite the frequent isolation of endospore-formers from marine sponges, little is known about the diversity and characterization of individual isolates. The main aims of this study were to isolate and characterize the spore-Forming Bacteria from the marine sponge Haliclona simulans and to examine their potential as a source for bioactive compounds. Methods and Results:  A bank of presumptive aerobic spore-Forming Bacteria was isolated from the marine sponge H. simulans. These represented c. 1% of the total culturable Bacterial population. A subgroup of thirty isolates was characterized using morphological, phenotypical and phylogenetic analysis. A large diversity of endospore-Forming Bacteria was present, with the thirty isolates being distributed through a variety of Bacillus and Paenibacillus species. These included ubiquitous species, such as B. subtilis, B. pumilus, B. licheniformis and B. cereus group, as well as species that are typically associated with marine habitats, such as B. aquimaris, B. algicola and B. hwajinpoensis. Two strains carried the aiiA gene that encodes a lactonase known to be able to disrupt quorum-sensing mechanisms, and various isolates demonstrated protease activity and antimicrobial activity against different pathogenic indicator strains, including Clostridium perfringens, Bacillus cereus and Listeria monocytogenes. Conclusions:  The marine sponge H. simulans harbours a diverse collection of endospore-Forming Bacteria, which produce proteases and antibiotics. This diversity appears to be overlooked by culture-dependent and culture-independent methods that do not specifically target sporeformers. Significance and Impact of Study:  Marine sponges are an as yet largely untapped and poorly understood source of endospore-Forming Bacterial diversity with potential biotechnological, biopharmaceutical and probiotic applications. These results also indicate the importance of combining different methodologies for the comprehensive characterization of complex microbial populations such as those found in marine sponges.

  • Diversity and bioactive potential of endospore‐Forming Bacteria cultured from the marine sponge Haliclona simulans
    Journal of applied microbiology, 2011
    Co-Authors: Robert W Phelan, Jonathan Kennedy, John P Morrissey, Alan D W Dobson, John O'halloran, Fergal O'gara, Teresa M Barbosa
    Abstract:

    Aims:  Despite the frequent isolation of endospore-formers from marine sponges, little is known about the diversity and characterization of individual isolates. The main aims of this study were to isolate and characterize the spore-Forming Bacteria from the marine sponge Haliclona simulans and to examine their potential as a source for bioactive compounds. Methods and Results:  A bank of presumptive aerobic spore-Forming Bacteria was isolated from the marine sponge H. simulans. These represented c. 1% of the total culturable Bacterial population. A subgroup of thirty isolates was characterized using morphological, phenotypical and phylogenetic analysis. A large diversity of endospore-Forming Bacteria was present, with the thirty isolates being distributed through a variety of Bacillus and Paenibacillus species. These included ubiquitous species, such as B. subtilis, B. pumilus, B. licheniformis and B. cereus group, as well as species that are typically associated with marine habitats, such as B. aquimaris, B. algicola and B. hwajinpoensis. Two strains carried the aiiA gene that encodes a lactonase known to be able to disrupt quorum-sensing mechanisms, and various isolates demonstrated protease activity and antimicrobial activity against different pathogenic indicator strains, including Clostridium perfringens, Bacillus cereus and Listeria monocytogenes. Conclusions:  The marine sponge H. simulans harbours a diverse collection of endospore-Forming Bacteria, which produce proteases and antibiotics. This diversity appears to be overlooked by culture-dependent and culture-independent methods that do not specifically target sporeformers. Significance and Impact of Study:  Marine sponges are an as yet largely untapped and poorly understood source of endospore-Forming Bacterial diversity with potential biotechnological, biopharmaceutical and probiotic applications. These results also indicate the importance of combining different methodologies for the comprehensive characterization of complex microbial populations such as those found in marine sponges.

James N. Palmer - One of the best experts on this subject based on the ideXlab platform.

  • biofilm Forming Bacteria and quality of life improvement after sinus surgery
    International Forum of Allergy & Rhinology, 2015
    Co-Authors: Zi Zhang, Nithin D. Adappa, Alexander G. Chiu, Laurel Doghramji, Noam A. Cohen, James N. Palmer
    Abstract:

    Background It remains unclear how much chronic rhinosinusitis (CRS) patients with Bacterial biofilms can benefit from functional endoscopic sinus surgery (FESS). We aimed to evaluate whether biofilm-Forming Bacteria was associated with quality of life (QOL) improvement after FESS. Methods This retrospective cohort study included adult CRS patients who underwent FESS from 2008 to 2011. Sinus samples were taken to evaluate for biofilm-formation in vitro using a modified Calgary Biofilm Detection Assay. QOL was measured before FESS, and 1-month, 3-month, and 6-month after FESS using 22-item Sino-Nasal Outcome Test (SNOT-22) scores. Patients’ characteristics and medications were collected. Clinical significant QOL change was defined as a difference of at least 0.5 standard deviation (SD) of baseline SNOT-22 score in the reference group. Results A total of 156 patients had complete data, and 15% had biofilm-Forming Bacteria (n = 24). Patients with biofilm-Forming Bacteria had significantly worse preoperative SNOT-22 scores compared to patients without biofilm-Forming Bacteria (48 ± 20 vs 38 ± 23, p = 0.048). Both groups had clinically significant QOL improvement after FESS, and the differences in their 1-month (23 ± 19 vs 17 ± 20) and 3-month (27 ± 18 vs 18 ± 19) post-FESS SNOT-22 scores were not significant. However, patients with biofilm-Forming Bacteria demonstrated significantly less QOL improvement than patients without biofilm-Forming Bacteria from pre-FESS to 6-month post-FESS visits after adjusting for clinical factors (35 ± 25 vs 14 ± 15; β-coefficient = 0.71; 95% confidence interval [CI], 0.13 to 1.28; p = 0.016). Conclusion CRS patients with biofilm-Forming Bacteria demonstrated clinically significant QOL improvement following FESS, but the degree of improvement was decreased overtime and became significantly worse than patients without biofilm-Forming Bacteria by 6-month follow-up. This QOL worsening was independent of other risk factors for CRS.

  • Biofilm‐Forming Bacteria and quality of life improvement after sinus surgery
    International forum of allergy & rhinology, 2015
    Co-Authors: Zi Zhang, Nithin D. Adappa, Alexander G. Chiu, Laurel Doghramji, Noam A. Cohen, James N. Palmer
    Abstract:

    Background It remains unclear how much chronic rhinosinusitis (CRS) patients with Bacterial biofilms can benefit from functional endoscopic sinus surgery (FESS). We aimed to evaluate whether biofilm-Forming Bacteria was associated with quality of life (QOL) improvement after FESS. Methods This retrospective cohort study included adult CRS patients who underwent FESS from 2008 to 2011. Sinus samples were taken to evaluate for biofilm-formation in vitro using a modified Calgary Biofilm Detection Assay. QOL was measured before FESS, and 1-month, 3-month, and 6-month after FESS using 22-item Sino-Nasal Outcome Test (SNOT-22) scores. Patients’ characteristics and medications were collected. Clinical significant QOL change was defined as a difference of at least 0.5 standard deviation (SD) of baseline SNOT-22 score in the reference group. Results A total of 156 patients had complete data, and 15% had biofilm-Forming Bacteria (n = 24). Patients with biofilm-Forming Bacteria had significantly worse preoperative SNOT-22 scores compared to patients without biofilm-Forming Bacteria (48 ± 20 vs 38 ± 23, p = 0.048). Both groups had clinically significant QOL improvement after FESS, and the differences in their 1-month (23 ± 19 vs 17 ± 20) and 3-month (27 ± 18 vs 18 ± 19) post-FESS SNOT-22 scores were not significant. However, patients with biofilm-Forming Bacteria demonstrated significantly less QOL improvement than patients without biofilm-Forming Bacteria from pre-FESS to 6-month post-FESS visits after adjusting for clinical factors (35 ± 25 vs 14 ± 15; β-coefficient = 0.71; 95% confidence interval [CI], 0.13 to 1.28; p = 0.016). Conclusion CRS patients with biofilm-Forming Bacteria demonstrated clinically significant QOL improvement following FESS, but the degree of improvement was decreased overtime and became significantly worse than patients without biofilm-Forming Bacteria by 6-month follow-up. This QOL worsening was independent of other risk factors for CRS.

Robert W Phelan - One of the best experts on this subject based on the ideXlab platform.

  • diversity and bioactive potential of endospore Forming Bacteria cultured from the marine sponge haliclona simulans
    Journal of Applied Microbiology, 2012
    Co-Authors: Robert W Phelan, John Ohalloran, Jonathan Kennedy, John P Morrissey, Alan D W Dobson, Fergal Ogara, Teresa M Barbosa
    Abstract:

    Aims:  Despite the frequent isolation of endospore-formers from marine sponges, little is known about the diversity and characterization of individual isolates. The main aims of this study were to isolate and characterize the spore-Forming Bacteria from the marine sponge Haliclona simulans and to examine their potential as a source for bioactive compounds. Methods and Results:  A bank of presumptive aerobic spore-Forming Bacteria was isolated from the marine sponge H. simulans. These represented c. 1% of the total culturable Bacterial population. A subgroup of thirty isolates was characterized using morphological, phenotypical and phylogenetic analysis. A large diversity of endospore-Forming Bacteria was present, with the thirty isolates being distributed through a variety of Bacillus and Paenibacillus species. These included ubiquitous species, such as B. subtilis, B. pumilus, B. licheniformis and B. cereus group, as well as species that are typically associated with marine habitats, such as B. aquimaris, B. algicola and B. hwajinpoensis. Two strains carried the aiiA gene that encodes a lactonase known to be able to disrupt quorum-sensing mechanisms, and various isolates demonstrated protease activity and antimicrobial activity against different pathogenic indicator strains, including Clostridium perfringens, Bacillus cereus and Listeria monocytogenes. Conclusions:  The marine sponge H. simulans harbours a diverse collection of endospore-Forming Bacteria, which produce proteases and antibiotics. This diversity appears to be overlooked by culture-dependent and culture-independent methods that do not specifically target sporeformers. Significance and Impact of Study:  Marine sponges are an as yet largely untapped and poorly understood source of endospore-Forming Bacterial diversity with potential biotechnological, biopharmaceutical and probiotic applications. These results also indicate the importance of combining different methodologies for the comprehensive characterization of complex microbial populations such as those found in marine sponges.

  • Diversity and bioactive potential of endospore‐Forming Bacteria cultured from the marine sponge Haliclona simulans
    Journal of applied microbiology, 2011
    Co-Authors: Robert W Phelan, Jonathan Kennedy, John P Morrissey, Alan D W Dobson, John O'halloran, Fergal O'gara, Teresa M Barbosa
    Abstract:

    Aims:  Despite the frequent isolation of endospore-formers from marine sponges, little is known about the diversity and characterization of individual isolates. The main aims of this study were to isolate and characterize the spore-Forming Bacteria from the marine sponge Haliclona simulans and to examine their potential as a source for bioactive compounds. Methods and Results:  A bank of presumptive aerobic spore-Forming Bacteria was isolated from the marine sponge H. simulans. These represented c. 1% of the total culturable Bacterial population. A subgroup of thirty isolates was characterized using morphological, phenotypical and phylogenetic analysis. A large diversity of endospore-Forming Bacteria was present, with the thirty isolates being distributed through a variety of Bacillus and Paenibacillus species. These included ubiquitous species, such as B. subtilis, B. pumilus, B. licheniformis and B. cereus group, as well as species that are typically associated with marine habitats, such as B. aquimaris, B. algicola and B. hwajinpoensis. Two strains carried the aiiA gene that encodes a lactonase known to be able to disrupt quorum-sensing mechanisms, and various isolates demonstrated protease activity and antimicrobial activity against different pathogenic indicator strains, including Clostridium perfringens, Bacillus cereus and Listeria monocytogenes. Conclusions:  The marine sponge H. simulans harbours a diverse collection of endospore-Forming Bacteria, which produce proteases and antibiotics. This diversity appears to be overlooked by culture-dependent and culture-independent methods that do not specifically target sporeformers. Significance and Impact of Study:  Marine sponges are an as yet largely untapped and poorly understood source of endospore-Forming Bacterial diversity with potential biotechnological, biopharmaceutical and probiotic applications. These results also indicate the importance of combining different methodologies for the comprehensive characterization of complex microbial populations such as those found in marine sponges.

Pilar Junier - One of the best experts on this subject based on the ideXlab platform.

  • endospore Forming Bacteria as new proxies to assess impact of eutrophication in lake geneva switzerland france
    Aquatic Sciences, 2014
    Co-Authors: Tina Wunderlin, Matthieu Bueche, Thomas Junier, Jean-luc Loizeau, Juan Pablo Corella, Stephanie Girardclos, Pilar Junier
    Abstract:

    Measurements of chemical composition and biological parameters of sediment cores are used as proxies for changes in past environmental conditions and more recently the human impact on ecosystem health. In this study, endospore-Forming Bacteria are proposed as a new biological proxy for such paleoecological reconstructions. A sediment core providing a record for the past 90 years (137Cs and magnetic susceptibility dating) was retrieved from the Rhone Delta of Lake Geneva. X-ray fluorescence was analyzed at a 0.2-cm resolution, while DNA extracts, elemental geochemistry and grain size were obtained at 4-cm intervals. The total number of Bacteria and endospore-Forming Bacteria were quantified by qPCR using the 16S rRNA gene and the endosporulation-specific spo0A gene. Furthermore, a spo0A fragment was subjected to amplicon sequencing to define OTUs (operational taxonomic units) and the phylogenetic affiliation of the endospore formers. The results showed that despite the fact that the quantity of extracted DNA decreased with the age of the sediment, the abundance of endospore-Forming Bacteria remained constant. However, the diversity of this group of Bacteria changed significantly, reflecting the eutrophication of the lake from 1960 to 1990. The shift in community composition was linked to the dominance of anaerobic clostridia-like endospore formers. This trend has reversed in the last 10 years of the record, suggesting a recovery after perturbation. This study shows that the abundance and diversity of endospore-Forming Bacteria can be used as proxies to reconstruct lake history. We hereby successfully introduce a new strategy for paleoecology that could also be applied to ocean sediments and long sediment cores.

  • Endospore-Forming Bacteria as new proxies to assess impact of eutrophication in Lake Geneva (Switzerland–France)
    Aquatic Sciences, 2013
    Co-Authors: Tina Wunderlin, Matthieu Bueche, Thomas Junier, Jean-luc Loizeau, Juan Pablo Corella, Stephanie Girardclos, Pilar Junier
    Abstract:

    Measurements of chemical composition and biological parameters of sediment cores are used as proxies for changes in past environmental conditions and more recently the human impact on ecosystem health. In this study, endospore-Forming Bacteria are proposed as a new biological proxy for such paleoecological reconstructions. A sediment core providing a record for the past 90 years (137Cs and magnetic susceptibility dating) was retrieved from the Rhone Delta of Lake Geneva. X-ray fluorescence was analyzed at a 0.2-cm resolution, while DNA extracts, elemental geochemistry and grain size were obtained at 4-cm intervals. The total number of Bacteria and endospore-Forming Bacteria were quantified by qPCR using the 16S rRNA gene and the endosporulation-specific spo0A gene. Furthermore, a spo0A fragment was subjected to amplicon sequencing to define OTUs (operational taxonomic units) and the phylogenetic affiliation of the endospore formers. The results showed that despite the fact that the quantity of extracted DNA decreased with the age of the sediment, the abundance of endospore-Forming Bacteria remained constant. However, the diversity of this group of Bacteria changed significantly, reflecting the eutrophication of the lake from 1960 to 1990. The shift in community composition was linked to the dominance of anaerobic clostridia-like endospore formers. This trend has reversed in the last 10 years of the record, suggesting a recovery after perturbation. This study shows that the abundance and diversity of endospore-Forming Bacteria can be used as proxies to reconstruct lake history. We hereby successfully introduce a new strategy for paleoecology that could also be applied to ocean sediments and long sediment cores.

  • Endospore-Forming Bacteria as an indicator of pollution in sediments of Lake Geneva
    E3S Web of Conferences, 2013
    Co-Authors: Matthieu Bueche, Loic Sauvain, Tina Wunderlin, Thomas Junier, M. Tercier, Matthieu Masson, E. Gascon, Jean-luc Loizeau, Pilar Junier
    Abstract:

    Treated wastewater and runoff-water is released by the outlet of the sewage treatment plant of Vidy (Lausanne) directly into the Lake of Geneva via a pipe located 300m from the shore. Even if this water is properly treated with modern technologies, we can observe an accumulation of micro pollutants into the sediments, and particularly heavy-metals. The main objective of this project is to investigate how these elevated concentrations of heavy metals affect both abundance and diversity of prokaryotes in the sediments. A special emphasis was given to endospore-Forming Bacteria, which could use sporulation as a survival strategy to resist in highly contaminated areas. This study could have implications both for understanding the role of endospore-Forming Bacteria in the environment as well as in terms of improving the bioremediation processes.

  • Endospore-Forming Bacteria as an indicator of pollution in sediments of
    2013
    Co-Authors: Lake Geneva, Matthieu Bueche, Loic Sauvain, Tina Wunderlin, Thomas Junier, M. Tercier, Matthieu Masson, E. Gascon, Pilar Junier
    Abstract:

    Treated wastewater and runoff-water is released by the outlet of the sewage treatment plant of Vidy (Lausanne) directly into the Lake of Geneva via a pipe located 300m from the shore. Even if this water is properly treated with modern technologies, we can observe an accumulation of micro pollutants into the sediments, and particularly heavy-metals. The main objective of this project is to investigate how these elevated concentrations of heavy metals affect both abundance and diversity of prokaryotes in the sediments. A special emphasis was given to endospore-Forming Bacteria, which could use sporulation as a survival strategy to resist in highly contaminated areas. This study could have implications both for understanding the role of endospore-Forming Bacteria in the environment as well as in terms of improving the bioremediation processes.

Zi Zhang - One of the best experts on this subject based on the ideXlab platform.

  • biofilm Forming Bacteria and quality of life improvement after sinus surgery
    International Forum of Allergy & Rhinology, 2015
    Co-Authors: Zi Zhang, Nithin D. Adappa, Alexander G. Chiu, Laurel Doghramji, Noam A. Cohen, James N. Palmer
    Abstract:

    Background It remains unclear how much chronic rhinosinusitis (CRS) patients with Bacterial biofilms can benefit from functional endoscopic sinus surgery (FESS). We aimed to evaluate whether biofilm-Forming Bacteria was associated with quality of life (QOL) improvement after FESS. Methods This retrospective cohort study included adult CRS patients who underwent FESS from 2008 to 2011. Sinus samples were taken to evaluate for biofilm-formation in vitro using a modified Calgary Biofilm Detection Assay. QOL was measured before FESS, and 1-month, 3-month, and 6-month after FESS using 22-item Sino-Nasal Outcome Test (SNOT-22) scores. Patients’ characteristics and medications were collected. Clinical significant QOL change was defined as a difference of at least 0.5 standard deviation (SD) of baseline SNOT-22 score in the reference group. Results A total of 156 patients had complete data, and 15% had biofilm-Forming Bacteria (n = 24). Patients with biofilm-Forming Bacteria had significantly worse preoperative SNOT-22 scores compared to patients without biofilm-Forming Bacteria (48 ± 20 vs 38 ± 23, p = 0.048). Both groups had clinically significant QOL improvement after FESS, and the differences in their 1-month (23 ± 19 vs 17 ± 20) and 3-month (27 ± 18 vs 18 ± 19) post-FESS SNOT-22 scores were not significant. However, patients with biofilm-Forming Bacteria demonstrated significantly less QOL improvement than patients without biofilm-Forming Bacteria from pre-FESS to 6-month post-FESS visits after adjusting for clinical factors (35 ± 25 vs 14 ± 15; β-coefficient = 0.71; 95% confidence interval [CI], 0.13 to 1.28; p = 0.016). Conclusion CRS patients with biofilm-Forming Bacteria demonstrated clinically significant QOL improvement following FESS, but the degree of improvement was decreased overtime and became significantly worse than patients without biofilm-Forming Bacteria by 6-month follow-up. This QOL worsening was independent of other risk factors for CRS.

  • Biofilm‐Forming Bacteria and quality of life improvement after sinus surgery
    International forum of allergy & rhinology, 2015
    Co-Authors: Zi Zhang, Nithin D. Adappa, Alexander G. Chiu, Laurel Doghramji, Noam A. Cohen, James N. Palmer
    Abstract:

    Background It remains unclear how much chronic rhinosinusitis (CRS) patients with Bacterial biofilms can benefit from functional endoscopic sinus surgery (FESS). We aimed to evaluate whether biofilm-Forming Bacteria was associated with quality of life (QOL) improvement after FESS. Methods This retrospective cohort study included adult CRS patients who underwent FESS from 2008 to 2011. Sinus samples were taken to evaluate for biofilm-formation in vitro using a modified Calgary Biofilm Detection Assay. QOL was measured before FESS, and 1-month, 3-month, and 6-month after FESS using 22-item Sino-Nasal Outcome Test (SNOT-22) scores. Patients’ characteristics and medications were collected. Clinical significant QOL change was defined as a difference of at least 0.5 standard deviation (SD) of baseline SNOT-22 score in the reference group. Results A total of 156 patients had complete data, and 15% had biofilm-Forming Bacteria (n = 24). Patients with biofilm-Forming Bacteria had significantly worse preoperative SNOT-22 scores compared to patients without biofilm-Forming Bacteria (48 ± 20 vs 38 ± 23, p = 0.048). Both groups had clinically significant QOL improvement after FESS, and the differences in their 1-month (23 ± 19 vs 17 ± 20) and 3-month (27 ± 18 vs 18 ± 19) post-FESS SNOT-22 scores were not significant. However, patients with biofilm-Forming Bacteria demonstrated significantly less QOL improvement than patients without biofilm-Forming Bacteria from pre-FESS to 6-month post-FESS visits after adjusting for clinical factors (35 ± 25 vs 14 ± 15; β-coefficient = 0.71; 95% confidence interval [CI], 0.13 to 1.28; p = 0.016). Conclusion CRS patients with biofilm-Forming Bacteria demonstrated clinically significant QOL improvement following FESS, but the degree of improvement was decreased overtime and became significantly worse than patients without biofilm-Forming Bacteria by 6-month follow-up. This QOL worsening was independent of other risk factors for CRS.