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Yoichi Honda - One of the best experts on this subject based on the ideXlab platform.

  • Identification of two mutations that cause defects in the ligninolytic system through an efficient Forward Genetics in the white‐rot agaricomycete Pleurotus ostreatus
    Environmental microbiology, 2017
    Co-Authors: Takehito Nakazawa, Ayako Izuno, Rina Kodera, Yasumasa Miyazaki, Masahiro Sakamoto, Yuji Isagi, Yoichi Honda
    Abstract:

    Summary White-rot fungi play an important role in the global carbon cycle because they are the species that almost exclusively biodegrade wood lignin in nature. Lignin peroxidases (LiPs), manganese peroxidases (MnPs), and versatile peroxidases (VPs) are considered key players in the ligninolytic system. Apart from LiPs, MnPs, and VPs, however, only few other factors involved in the ligninolytic system have been investigated using molecular Genetics, implying the existence of unidentified elements. By combining classical genetic techniques with next-generation sequencing technology, we successfully showed an efficient Forward Genetics approach to identify mutations causing defects in the ligninolytic system of the white-rot fungus Pleurotus ostreatus. In this study, we identified two genes—chd1 and wtr1—mutations in which cause an almost complete loss of Mn2+-dependent peroxidase activity. The chd1 gene encodes a putative chromatin modifier, and wtr1 encodes an agaricomycete-specific protein with a putative DNA-binding domain. The chd1-1 mutation and targeted disruption of wtr1 hamper the ability of P. ostreatus to biodegrade wood lignin. Examination of the effects of the aforementioned mutation and disruption on the expression of certain MnP/VP genes suggests that a complex mechanism underlies the ligninolytic system in P. ostreatus. This article is protected by copyright. All rights reserved.

  • identification of two mutations that cause defects in the ligninolytic system through an efficient Forward Genetics in the white rot agaricomycete pleurotus ostreatus
    Environmental Microbiology, 2017
    Co-Authors: Takehito Nakazawa, Ayako Izuno, Rina Kodera, Yasumasa Miyazaki, Masahiro Sakamoto, Yuji Isagi, Yoichi Honda
    Abstract:

    Summary White-rot fungi play an important role in the global carbon cycle because they are the species that almost exclusively biodegrade wood lignin in nature. Lignin peroxidases (LiPs), manganese peroxidases (MnPs), and versatile peroxidases (VPs) are considered key players in the ligninolytic system. Apart from LiPs, MnPs, and VPs, however, only few other factors involved in the ligninolytic system have been investigated using molecular Genetics, implying the existence of unidentified elements. By combining classical genetic techniques with next-generation sequencing technology, we successfully showed an efficient Forward Genetics approach to identify mutations causing defects in the ligninolytic system of the white-rot fungus Pleurotus ostreatus. In this study, we identified two genes—chd1 and wtr1—mutations in which cause an almost complete loss of Mn2+-dependent peroxidase activity. The chd1 gene encodes a putative chromatin modifier, and wtr1 encodes an agaricomycete-specific protein with a putative DNA-binding domain. The chd1-1 mutation and targeted disruption of wtr1 hamper the ability of P. ostreatus to biodegrade wood lignin. Examination of the effects of the aforementioned mutation and disruption on the expression of certain MnP/VP genes suggests that a complex mechanism underlies the ligninolytic system in P. ostreatus. This article is protected by copyright. All rights reserved.

Takehito Nakazawa - One of the best experts on this subject based on the ideXlab platform.

  • Identification of two mutations that cause defects in the ligninolytic system through an efficient Forward Genetics in the white‐rot agaricomycete Pleurotus ostreatus
    Environmental microbiology, 2017
    Co-Authors: Takehito Nakazawa, Ayako Izuno, Rina Kodera, Yasumasa Miyazaki, Masahiro Sakamoto, Yuji Isagi, Yoichi Honda
    Abstract:

    Summary White-rot fungi play an important role in the global carbon cycle because they are the species that almost exclusively biodegrade wood lignin in nature. Lignin peroxidases (LiPs), manganese peroxidases (MnPs), and versatile peroxidases (VPs) are considered key players in the ligninolytic system. Apart from LiPs, MnPs, and VPs, however, only few other factors involved in the ligninolytic system have been investigated using molecular Genetics, implying the existence of unidentified elements. By combining classical genetic techniques with next-generation sequencing technology, we successfully showed an efficient Forward Genetics approach to identify mutations causing defects in the ligninolytic system of the white-rot fungus Pleurotus ostreatus. In this study, we identified two genes—chd1 and wtr1—mutations in which cause an almost complete loss of Mn2+-dependent peroxidase activity. The chd1 gene encodes a putative chromatin modifier, and wtr1 encodes an agaricomycete-specific protein with a putative DNA-binding domain. The chd1-1 mutation and targeted disruption of wtr1 hamper the ability of P. ostreatus to biodegrade wood lignin. Examination of the effects of the aforementioned mutation and disruption on the expression of certain MnP/VP genes suggests that a complex mechanism underlies the ligninolytic system in P. ostreatus. This article is protected by copyright. All rights reserved.

  • identification of two mutations that cause defects in the ligninolytic system through an efficient Forward Genetics in the white rot agaricomycete pleurotus ostreatus
    Environmental Microbiology, 2017
    Co-Authors: Takehito Nakazawa, Ayako Izuno, Rina Kodera, Yasumasa Miyazaki, Masahiro Sakamoto, Yuji Isagi, Yoichi Honda
    Abstract:

    Summary White-rot fungi play an important role in the global carbon cycle because they are the species that almost exclusively biodegrade wood lignin in nature. Lignin peroxidases (LiPs), manganese peroxidases (MnPs), and versatile peroxidases (VPs) are considered key players in the ligninolytic system. Apart from LiPs, MnPs, and VPs, however, only few other factors involved in the ligninolytic system have been investigated using molecular Genetics, implying the existence of unidentified elements. By combining classical genetic techniques with next-generation sequencing technology, we successfully showed an efficient Forward Genetics approach to identify mutations causing defects in the ligninolytic system of the white-rot fungus Pleurotus ostreatus. In this study, we identified two genes—chd1 and wtr1—mutations in which cause an almost complete loss of Mn2+-dependent peroxidase activity. The chd1 gene encodes a putative chromatin modifier, and wtr1 encodes an agaricomycete-specific protein with a putative DNA-binding domain. The chd1-1 mutation and targeted disruption of wtr1 hamper the ability of P. ostreatus to biodegrade wood lignin. Examination of the effects of the aforementioned mutation and disruption on the expression of certain MnP/VP genes suggests that a complex mechanism underlies the ligninolytic system in P. ostreatus. This article is protected by copyright. All rights reserved.

Yuji Isagi - One of the best experts on this subject based on the ideXlab platform.

  • Identification of two mutations that cause defects in the ligninolytic system through an efficient Forward Genetics in the white‐rot agaricomycete Pleurotus ostreatus
    Environmental microbiology, 2017
    Co-Authors: Takehito Nakazawa, Ayako Izuno, Rina Kodera, Yasumasa Miyazaki, Masahiro Sakamoto, Yuji Isagi, Yoichi Honda
    Abstract:

    Summary White-rot fungi play an important role in the global carbon cycle because they are the species that almost exclusively biodegrade wood lignin in nature. Lignin peroxidases (LiPs), manganese peroxidases (MnPs), and versatile peroxidases (VPs) are considered key players in the ligninolytic system. Apart from LiPs, MnPs, and VPs, however, only few other factors involved in the ligninolytic system have been investigated using molecular Genetics, implying the existence of unidentified elements. By combining classical genetic techniques with next-generation sequencing technology, we successfully showed an efficient Forward Genetics approach to identify mutations causing defects in the ligninolytic system of the white-rot fungus Pleurotus ostreatus. In this study, we identified two genes—chd1 and wtr1—mutations in which cause an almost complete loss of Mn2+-dependent peroxidase activity. The chd1 gene encodes a putative chromatin modifier, and wtr1 encodes an agaricomycete-specific protein with a putative DNA-binding domain. The chd1-1 mutation and targeted disruption of wtr1 hamper the ability of P. ostreatus to biodegrade wood lignin. Examination of the effects of the aforementioned mutation and disruption on the expression of certain MnP/VP genes suggests that a complex mechanism underlies the ligninolytic system in P. ostreatus. This article is protected by copyright. All rights reserved.

  • identification of two mutations that cause defects in the ligninolytic system through an efficient Forward Genetics in the white rot agaricomycete pleurotus ostreatus
    Environmental Microbiology, 2017
    Co-Authors: Takehito Nakazawa, Ayako Izuno, Rina Kodera, Yasumasa Miyazaki, Masahiro Sakamoto, Yuji Isagi, Yoichi Honda
    Abstract:

    Summary White-rot fungi play an important role in the global carbon cycle because they are the species that almost exclusively biodegrade wood lignin in nature. Lignin peroxidases (LiPs), manganese peroxidases (MnPs), and versatile peroxidases (VPs) are considered key players in the ligninolytic system. Apart from LiPs, MnPs, and VPs, however, only few other factors involved in the ligninolytic system have been investigated using molecular Genetics, implying the existence of unidentified elements. By combining classical genetic techniques with next-generation sequencing technology, we successfully showed an efficient Forward Genetics approach to identify mutations causing defects in the ligninolytic system of the white-rot fungus Pleurotus ostreatus. In this study, we identified two genes—chd1 and wtr1—mutations in which cause an almost complete loss of Mn2+-dependent peroxidase activity. The chd1 gene encodes a putative chromatin modifier, and wtr1 encodes an agaricomycete-specific protein with a putative DNA-binding domain. The chd1-1 mutation and targeted disruption of wtr1 hamper the ability of P. ostreatus to biodegrade wood lignin. Examination of the effects of the aforementioned mutation and disruption on the expression of certain MnP/VP genes suggests that a complex mechanism underlies the ligninolytic system in P. ostreatus. This article is protected by copyright. All rights reserved.

Masahiro Sakamoto - One of the best experts on this subject based on the ideXlab platform.

  • Identification of two mutations that cause defects in the ligninolytic system through an efficient Forward Genetics in the white‐rot agaricomycete Pleurotus ostreatus
    Environmental microbiology, 2017
    Co-Authors: Takehito Nakazawa, Ayako Izuno, Rina Kodera, Yasumasa Miyazaki, Masahiro Sakamoto, Yuji Isagi, Yoichi Honda
    Abstract:

    Summary White-rot fungi play an important role in the global carbon cycle because they are the species that almost exclusively biodegrade wood lignin in nature. Lignin peroxidases (LiPs), manganese peroxidases (MnPs), and versatile peroxidases (VPs) are considered key players in the ligninolytic system. Apart from LiPs, MnPs, and VPs, however, only few other factors involved in the ligninolytic system have been investigated using molecular Genetics, implying the existence of unidentified elements. By combining classical genetic techniques with next-generation sequencing technology, we successfully showed an efficient Forward Genetics approach to identify mutations causing defects in the ligninolytic system of the white-rot fungus Pleurotus ostreatus. In this study, we identified two genes—chd1 and wtr1—mutations in which cause an almost complete loss of Mn2+-dependent peroxidase activity. The chd1 gene encodes a putative chromatin modifier, and wtr1 encodes an agaricomycete-specific protein with a putative DNA-binding domain. The chd1-1 mutation and targeted disruption of wtr1 hamper the ability of P. ostreatus to biodegrade wood lignin. Examination of the effects of the aforementioned mutation and disruption on the expression of certain MnP/VP genes suggests that a complex mechanism underlies the ligninolytic system in P. ostreatus. This article is protected by copyright. All rights reserved.

  • identification of two mutations that cause defects in the ligninolytic system through an efficient Forward Genetics in the white rot agaricomycete pleurotus ostreatus
    Environmental Microbiology, 2017
    Co-Authors: Takehito Nakazawa, Ayako Izuno, Rina Kodera, Yasumasa Miyazaki, Masahiro Sakamoto, Yuji Isagi, Yoichi Honda
    Abstract:

    Summary White-rot fungi play an important role in the global carbon cycle because they are the species that almost exclusively biodegrade wood lignin in nature. Lignin peroxidases (LiPs), manganese peroxidases (MnPs), and versatile peroxidases (VPs) are considered key players in the ligninolytic system. Apart from LiPs, MnPs, and VPs, however, only few other factors involved in the ligninolytic system have been investigated using molecular Genetics, implying the existence of unidentified elements. By combining classical genetic techniques with next-generation sequencing technology, we successfully showed an efficient Forward Genetics approach to identify mutations causing defects in the ligninolytic system of the white-rot fungus Pleurotus ostreatus. In this study, we identified two genes—chd1 and wtr1—mutations in which cause an almost complete loss of Mn2+-dependent peroxidase activity. The chd1 gene encodes a putative chromatin modifier, and wtr1 encodes an agaricomycete-specific protein with a putative DNA-binding domain. The chd1-1 mutation and targeted disruption of wtr1 hamper the ability of P. ostreatus to biodegrade wood lignin. Examination of the effects of the aforementioned mutation and disruption on the expression of certain MnP/VP genes suggests that a complex mechanism underlies the ligninolytic system in P. ostreatus. This article is protected by copyright. All rights reserved.

Yasumasa Miyazaki - One of the best experts on this subject based on the ideXlab platform.

  • Identification of two mutations that cause defects in the ligninolytic system through an efficient Forward Genetics in the white‐rot agaricomycete Pleurotus ostreatus
    Environmental microbiology, 2017
    Co-Authors: Takehito Nakazawa, Ayako Izuno, Rina Kodera, Yasumasa Miyazaki, Masahiro Sakamoto, Yuji Isagi, Yoichi Honda
    Abstract:

    Summary White-rot fungi play an important role in the global carbon cycle because they are the species that almost exclusively biodegrade wood lignin in nature. Lignin peroxidases (LiPs), manganese peroxidases (MnPs), and versatile peroxidases (VPs) are considered key players in the ligninolytic system. Apart from LiPs, MnPs, and VPs, however, only few other factors involved in the ligninolytic system have been investigated using molecular Genetics, implying the existence of unidentified elements. By combining classical genetic techniques with next-generation sequencing technology, we successfully showed an efficient Forward Genetics approach to identify mutations causing defects in the ligninolytic system of the white-rot fungus Pleurotus ostreatus. In this study, we identified two genes—chd1 and wtr1—mutations in which cause an almost complete loss of Mn2+-dependent peroxidase activity. The chd1 gene encodes a putative chromatin modifier, and wtr1 encodes an agaricomycete-specific protein with a putative DNA-binding domain. The chd1-1 mutation and targeted disruption of wtr1 hamper the ability of P. ostreatus to biodegrade wood lignin. Examination of the effects of the aforementioned mutation and disruption on the expression of certain MnP/VP genes suggests that a complex mechanism underlies the ligninolytic system in P. ostreatus. This article is protected by copyright. All rights reserved.

  • identification of two mutations that cause defects in the ligninolytic system through an efficient Forward Genetics in the white rot agaricomycete pleurotus ostreatus
    Environmental Microbiology, 2017
    Co-Authors: Takehito Nakazawa, Ayako Izuno, Rina Kodera, Yasumasa Miyazaki, Masahiro Sakamoto, Yuji Isagi, Yoichi Honda
    Abstract:

    Summary White-rot fungi play an important role in the global carbon cycle because they are the species that almost exclusively biodegrade wood lignin in nature. Lignin peroxidases (LiPs), manganese peroxidases (MnPs), and versatile peroxidases (VPs) are considered key players in the ligninolytic system. Apart from LiPs, MnPs, and VPs, however, only few other factors involved in the ligninolytic system have been investigated using molecular Genetics, implying the existence of unidentified elements. By combining classical genetic techniques with next-generation sequencing technology, we successfully showed an efficient Forward Genetics approach to identify mutations causing defects in the ligninolytic system of the white-rot fungus Pleurotus ostreatus. In this study, we identified two genes—chd1 and wtr1—mutations in which cause an almost complete loss of Mn2+-dependent peroxidase activity. The chd1 gene encodes a putative chromatin modifier, and wtr1 encodes an agaricomycete-specific protein with a putative DNA-binding domain. The chd1-1 mutation and targeted disruption of wtr1 hamper the ability of P. ostreatus to biodegrade wood lignin. Examination of the effects of the aforementioned mutation and disruption on the expression of certain MnP/VP genes suggests that a complex mechanism underlies the ligninolytic system in P. ostreatus. This article is protected by copyright. All rights reserved.