Free-Living Nematodes

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Wenjun Liang - One of the best experts on this subject based on the ideXlab platform.

  • Do shifts in soil Oribatida (Acari, Oribatida) give information on differences in fruit yield of Chinese star anise?
    Agriculture Ecosystems & Environment, 2015
    Co-Authors: Bing Yang, Xianghui Liu, Weikai Bao, Wenjun Liang
    Abstract:

    Abstract Oribatida are actively involved in organic matter decomposition, nutrient cycling, and soil formation. However, the effect of their activities on the fruit yield of Chinese star anise (Illicium verum) is unknown. Here, Oribatida in the topsoils of low- and high-yielding plantations of star anise were extracted with Berlese–Tullgren funnels from soil samples collected in September, 2012, and their abundances, diversities, and compositions were compared. Fruit yield exerted a negligible effect on abundance and diversity of Oribatida. However, the Oribatida community compositions in the two plantation types were distinct. Multivariate analysis indicated that the combined effects of yield difference and environmental factors, including soil physicochemical properties, soil microbes, and Free-Living Nematodes, accounted for the differences in Oribatida community compositions. Indicator species analysis suggested that Tectocepheus, Rostrozetes, Hypochthonius, and Microzetes were typically found in the high-yielding plantation. The results demonstrated that soil physicochemical properties and trophic interactions among components in the soil food web were important factors affecting the community compositions of the Oribatida mites.

Bing Yang - One of the best experts on this subject based on the ideXlab platform.

  • Do shifts in soil Oribatida (Acari, Oribatida) give information on differences in fruit yield of Chinese star anise?
    Agriculture Ecosystems & Environment, 2015
    Co-Authors: Bing Yang, Xianghui Liu, Weikai Bao, Wenjun Liang
    Abstract:

    Abstract Oribatida are actively involved in organic matter decomposition, nutrient cycling, and soil formation. However, the effect of their activities on the fruit yield of Chinese star anise (Illicium verum) is unknown. Here, Oribatida in the topsoils of low- and high-yielding plantations of star anise were extracted with Berlese–Tullgren funnels from soil samples collected in September, 2012, and their abundances, diversities, and compositions were compared. Fruit yield exerted a negligible effect on abundance and diversity of Oribatida. However, the Oribatida community compositions in the two plantation types were distinct. Multivariate analysis indicated that the combined effects of yield difference and environmental factors, including soil physicochemical properties, soil microbes, and Free-Living Nematodes, accounted for the differences in Oribatida community compositions. Indicator species analysis suggested that Tectocepheus, Rostrozetes, Hypochthonius, and Microzetes were typically found in the high-yielding plantation. The results demonstrated that soil physicochemical properties and trophic interactions among components in the soil food web were important factors affecting the community compositions of the Oribatida mites.

Xianghui Liu - One of the best experts on this subject based on the ideXlab platform.

  • Do shifts in soil Oribatida (Acari, Oribatida) give information on differences in fruit yield of Chinese star anise?
    Agriculture Ecosystems & Environment, 2015
    Co-Authors: Bing Yang, Xianghui Liu, Weikai Bao, Wenjun Liang
    Abstract:

    Abstract Oribatida are actively involved in organic matter decomposition, nutrient cycling, and soil formation. However, the effect of their activities on the fruit yield of Chinese star anise (Illicium verum) is unknown. Here, Oribatida in the topsoils of low- and high-yielding plantations of star anise were extracted with Berlese–Tullgren funnels from soil samples collected in September, 2012, and their abundances, diversities, and compositions were compared. Fruit yield exerted a negligible effect on abundance and diversity of Oribatida. However, the Oribatida community compositions in the two plantation types were distinct. Multivariate analysis indicated that the combined effects of yield difference and environmental factors, including soil physicochemical properties, soil microbes, and Free-Living Nematodes, accounted for the differences in Oribatida community compositions. Indicator species analysis suggested that Tectocepheus, Rostrozetes, Hypochthonius, and Microzetes were typically found in the high-yielding plantation. The results demonstrated that soil physicochemical properties and trophic interactions among components in the soil food web were important factors affecting the community compositions of the Oribatida mites.

Weikai Bao - One of the best experts on this subject based on the ideXlab platform.

  • Do shifts in soil Oribatida (Acari, Oribatida) give information on differences in fruit yield of Chinese star anise?
    Agriculture Ecosystems & Environment, 2015
    Co-Authors: Bing Yang, Xianghui Liu, Weikai Bao, Wenjun Liang
    Abstract:

    Abstract Oribatida are actively involved in organic matter decomposition, nutrient cycling, and soil formation. However, the effect of their activities on the fruit yield of Chinese star anise (Illicium verum) is unknown. Here, Oribatida in the topsoils of low- and high-yielding plantations of star anise were extracted with Berlese–Tullgren funnels from soil samples collected in September, 2012, and their abundances, diversities, and compositions were compared. Fruit yield exerted a negligible effect on abundance and diversity of Oribatida. However, the Oribatida community compositions in the two plantation types were distinct. Multivariate analysis indicated that the combined effects of yield difference and environmental factors, including soil physicochemical properties, soil microbes, and Free-Living Nematodes, accounted for the differences in Oribatida community compositions. Indicator species analysis suggested that Tectocepheus, Rostrozetes, Hypochthonius, and Microzetes were typically found in the high-yielding plantation. The results demonstrated that soil physicochemical properties and trophic interactions among components in the soil food web were important factors affecting the community compositions of the Oribatida mites.

James P Mccarter - One of the best experts on this subject based on the ideXlab platform.

  • comparative genomics of gene expression in the parasitic and free living Nematodes strongyloides stercoralis and caenorhabditis elegans
    Journal of Agriculture and Plant Sciences, 2013
    Co-Authors: Makedonka Mitreva, John Martin, Todd Wylie, Sandra W Clifton, James P Mccarter, Mike Dante, Brandi Chiapelli, Deana Pape, Thomas B Nutman, Robert H Waterston
    Abstract:

    While developmental timing of gene expression is used to infer potential genefunction, studies have yet to correlate this information between species. Weanalyzed 10,921 ESTs in 3,311 clusters from first and infective third stagelarva (L1, L3i) of the parasitic nematode Strongyloides stercoralis andcompared the results to Caenorhabditis elegans, a species that has an L3i-likedauer stage. Comparing S. stercoralis clusters with stage-specific expression toC. elegans homologs expressed in either dauer or non-dauer stages, matchesbetween S. stercoralis L1 and C. elegans non-dauer expressed genesdominated, suggesting conservation in the repertoire of genes expressed duringgrowth in nutrient-rich conditions. For example, S. stercoralis collagentranscripts were abundant in L1 but not L3i, a pattern consistent with C.elegans collagens. While a greater proportion of S. stercoralis L3i than L1genes have homologs among the C. elegans dauer-specific transcripts, we didnot uncover evidence for a robust conserved L3i / dauer ‘expression signature’.Strikingly, in comparisons of S. stercoralis clusters to C. elegans homologswith RNAi knockouts, those with significant L1-specific expression were morethan twice as likely as L3i-specific clusters to match genes with phenotypes.We also provide functional classifications of S. stercoralis clusters.

  • comparative genomics of gene expression in the parasitic and free living Nematodes strongyloides stercoralis and caenorhabditis elegans
    Genome Research, 2004
    Co-Authors: Makedonka Mitreva, John Martin, Todd Wylie, Sandra W Clifton, James P Mccarter, Mike Dante, Brandi Chiapelli, Deana Pape, Thomas B Nutman, Robert H Waterston
    Abstract:

    Although developmental timing of gene expression is used to infer potential gene function, studies have yet to correlate this information between species. We analyzed 10,921 ESTs in 3311 clusters from first- and infective third-stage larva (L1, L3i) of the parasitic nematode Strongyloides stercoralis and compared the results to Caenorhabditis elegans, a species that has an L3i-like dauer stage. In the comparison of S. stercoralis clusters with stage-specific expression to C. elegans homologs expressed in either dauer or nondauer stages, matches between S. stercoralis L1 and C. elegans nondauer-expressed genes dominated, suggesting conservation in the repertoire of genes expressed during growth in nutrient-rich conditions. For example, S. stercoralis collagen transcripts were abundant in L1 but not L3i, a pattern consistent with C. elegans collagens. Although a greater proportion of S. stercoralis L3i than L1 genes have homologs among the C. elegans dauer-specific transcripts, we did not uncover evidence of a robust conserved L3i/dauer ‘expression signature.’ Strikingly, in comparisons of S. stercoralis clusters to C. elegans homologs with RNAi knockouts, those with significant L1-specific expression were more than twice as likely as L3i-specific clusters to match genes with phenotypes. We also provide functional classifications of S. stercoralis clusters.

  • nematode net a tool for navigating sequences from parasitic and free living Nematodes
    Nucleic Acids Research, 2004
    Co-Authors: Todd Wylie, Makedonka Mitreva, John Martin, Michael Dante, Sandra W Clifton, Asif T Chinwalla, Robert H Waterston, Richard K Wilson, James P Mccarter
    Abstract:

    Nematode.net (www.nematode.net) is a webaccessible resource for investigating gene sequences from nematode genomes. The database is an outgrowth of the parasitic nematode EST project at Washington University’s Genome Sequencing Center (GSC), St Louis. A sister project at the University of Edinburgh and the Sanger Institute is also underway. More than 295 000 ESTs have been generated from >30 Nematodes other than Caenorhabditis elegans including key parasites of humans, animals and plants. Nematode.net currently provides NemaGene EST cluster consensus sequence, enhanced online BLAST search tools, functional classifications of cluster sequences and comprehensive information concerning the ongoing generation of nematode genome data. The long-term goal of nematode.net is to provide the scientific community with the highest quality sequence information and tools for studying these diverse species.