The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Elissa A. Hallem - One of the best experts on this subject based on the ideXlab platform.
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Strongyloides rna seq browser a web based software platform for on demand bioinformatics analyses of Strongyloides species
G3: Genes Genomes Genetics, 2021Co-Authors: Astra S. Bryant, Stephanie F Demarco, Elissa A. HallemAbstract:Soil-transmitted gastrointestinal parasitic nematodes infect approximately 1 billion people worldwide, predominantly in low-resource communities. Skin-penetrating gastrointestinal nematodes in the genus Strongyloides are emerging as model systems for mechanistic studies of soil-transmitted helminths due to the growing availability of functional genomics tools for these species. To facilitate future genomics studies of Strongyloides species, we have designed a web-based application, the Strongyloides RNA-seq Browser, that provides an open source, user-friendly portal for accessing and analyzing Strongyloides genomic expression data. Specifically, the Strongyloides RNA-seq Browser takes advantage of alignment-free read mapping tools and R-based transcriptomics tools to re-analyze publicly available RNA sequencing datasets from four Strongyloides species: Strongyloides stercoralis, Strongyloides ratti, Strongyloides papillosus, and Strongyloides venezuelensis. This application permits on-demand exploration and quantification of gene expression across life stages without requiring previous coding experience. Here, we describe this interactive application and demonstrate how it may be used by nematode researchers to conduct a standard set of bioinformatics queries.
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terror in the dirt sensory determinants of host seeking in soil transmitted mammalian parasitic nematodes
International Journal for Parasitology-Drugs and Drug Resistance, 2018Co-Authors: Astra S. Bryant, Elissa A. HallemAbstract:Abstract Infection with gastrointestinal parasitic nematodes is a major cause of chronic morbidity and economic burden around the world, particularly in low-resource settings. Some parasitic nematode species, including the human-parasitic threadworm Strongyloides stercoralis and human-parasitic hookworms in the genera Ancylostoma and Necator, feature a soil-dwelling infective larval stage that seeks out hosts for infection using a variety of host-emitted sensory cues. Here, we review our current understanding of the behavioral responses of soil-dwelling infective larvae to host-emitted sensory cues, and the molecular and cellular mechanisms that mediate these responses. We also discuss the development of methods for transgenesis and CRISPR/Cas9-mediated targeted mutagenesis in Strongyloides stercoralis and the closely related rat parasite Strongyloides ratti. These methods have established S. stercoralis and S. ratti as genetic model systems for gastrointestinal parasitic nematodes and are enabling more detailed investigations into the neural mechanisms that underlie the sensory-driven behaviors of this medically and economically important class of parasites.
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Terror in the dirt: Sensory determinants of host seeking in soil-transmitted mammalian-parasitic nematodes
Elsevier, 2018Co-Authors: Astra S. Bryant, Elissa A. HallemAbstract:Infection with gastrointestinal parasitic nematodes is a major cause of chronic morbidity and economic burden around the world, particularly in low-resource settings. Some parasitic nematode species, including the human-parasitic threadworm Strongyloides stercoralis and human-parasitic hookworms in the genera Ancylostoma and Necator, feature a soil-dwelling infective larval stage that seeks out hosts for infection using a variety of host-emitted sensory cues. Here, we review our current understanding of the behavioral responses of soil-dwelling infective larvae to host-emitted sensory cues, and the molecular and cellular mechanisms that mediate these responses. We also discuss the development of methods for transgenesis and CRISPR/Cas9-mediated targeted mutagenesis in Strongyloides stercoralis and the closely related rat parasite Strongyloides ratti. These methods have established S. stercoralis and S. ratti as genetic model systems for gastrointestinal parasitic nematodes and are enabling more detailed investigations into the neural mechanisms that underlie the sensory-driven behaviors of this medically and economically important class of parasites. Keywords: Parasitic helminth, Parasitic nematode, Host seeking, Chemosensation, Thermosensation, Sensory behavior, Strongyloide
Camber Ana-marija - One of the best experts on this subject based on the ideXlab platform.
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Natural occurrence of Strongyloides spp. in vervet monkeys (Chlorocebus pygerythrus) in Kuti Wildlife reserve, Malawi
2020Co-Authors: Camber Ana-marijaAbstract:The geographic overlap between non-human primates and people is increasing, especially in tropical and subtropical countries. This inevitably creates a potential for anthropogenic and zoonotic transmission of parasites, which could harm both wildlife and human communities. Strongyloides spp. are obligate gastrointestinal nematodes that are relatively host specific, but transmission between species can occur. It is estimated that between 30-100 million people are infected by Strongyloides spp. worldwide. In this study the ubiquitous non-human primate Chlorocebus pygrerythrus was used as a model to investigate the relationship between anthropogenic disturbance and Strongyloides spp. infection. Chlorocebus pygrerythrus, more commonly known as vervet monkeys, are medium sized monkeys found in the east and south of Africa. They are highly adaptive to different habitats and are found in semi-deserts, snowy mountains, savannah to more anthropogenic environments such as villages and cities. Vervet monkeys spend a large amount of time foraging on the ground, making them susceptible to soiltransmitted helminths. The present study investigated the natural occurrence of Strongyloides spp. in two vervet monkey troops in Kuti Wildlife Reserve, Malawi. Faecal samples from each troop were analysed (n=60) and the results showed a significantly higher prevalence (p
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Natural occurrence of Strongyloides spp. in vervet monkeys (Chlorocebus pygerythrus) in Kuti Wildlife reserve, Malawi : assessment of correlation between anthropogenic disturbance and Strongyloides spp. infection
SLU Dept. of Clinical Sciences, 2020Co-Authors: Camber Ana-marijaAbstract:The geographic overlap between non-human primates and people is increasing, especially in tropical and subtropical countries. This inevitably creates a potential for anthropogenic and zoonotic transmission of parasites, which could harm both wildlife and human communities. Strongyloides spp. are obligate gastrointestinal nematodes that are relatively host specific, but transmission between species can occur. It is estimated that between 30-100 million people are infected by Strongyloides spp. worldwide. In this study the ubiquitous non-human primate Chlorocebus pygrerythrus was used as a model to investigate the relationship between anthropogenic disturbance and Strongyloides spp. infection. Chlorocebus pygrerythrus, more commonly known as vervet monkeys, are medium sized monkeys found in the east and south of Africa. They are highly adaptive to different habitats and are found in semi-deserts, snowy mountains, savannah to more anthropogenic environments such as villages and cities. Vervet monkeys spend a large amount of time foraging on the ground, making them susceptible to soiltransmitted helminths. The present study investigated the natural occurrence of Strongyloides spp. in two vervet monkey troops in Kuti Wildlife Reserve, Malawi. Faecal samples from each troop were analysed (n=60) and the results showed a significantly higher prevalence (p
Agnaldo Soares Lima - One of the best experts on this subject based on the ideXlab platform.
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Strongyloides stercoralis hyperinfection syndrome after liver transplantation case report and literature review
Transplant Infectious Disease, 2009Co-Authors: Eduardo Garcia Vilela, Wanessa Trindade Clemente, R R L Mira, Henrique O G Torres, Luiz Fernando Veloso, L P Fonseca, L R De Carvalho E Fonseca, M D C Franca, Agnaldo Soares LimaAbstract:Strongyloides stercoralis is an intestinal nematode that causes human infections and whose life cycle has special features, including autoinfection. Strongyloides infection may be asymptomatic for years, owing to a low parasite load. During immunosuppressive therapy, however, if cellular immunity is depressed, autoinfection can occur at a higher rate, resulting in hyperinfection syndrome. In this specific circumstance, it can become a fatal illness. We describe a case of hyperinfection syndrome in a liver transplant recipient and also review the literature.
Jie Wang - One of the best experts on this subject based on the ideXlab platform.
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a fatal Strongyloides stercoralis hyperinfection syndrome in a patient with chronic kidney disease a case report and literature review
Medicine, 2016Co-Authors: Qing Yang, Jie WangAbstract:Strongyloides stercoralis hyperinfection syndrome is a rare but fatal disease, which occurs commonly in immunocompromised patients. Strongyloidiasis among patients with chronic kidney disease is rarely reported.A 55-year-old Chinese male presented to hospital with diarrhea and abdominal pain. He developed acute respiratory failure and progressed to diffuse alveolar hemorrhage owing to disseminated strongyloidiasis immediately. The bronchoalveolar lavage revealed filariform larvae of Strongyloides stercoralis.This patient was diagnosed with Strongyloides hyperinfection syndrome. Although albendazole, mechanical ventilator support, fluid resuscitation, vasopressor support, extracorporeal membrane oxygenation, hydrocortisone, and broadspectrum antimicrobials were actively used, the patient eventually died.Similar cases in patients with chronic kidney disease in the literature are also reviewed. Through literature review, we recommend that strongyloidiasis should be routinely investigated in patients with chronic kidney disease who will undergo immunosuppressive therapy.
Noah Craft - One of the best experts on this subject based on the ideXlab platform.
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disseminated Strongyloides stercoralis hyperinfection during medical immunosuppression
Journal of The American Academy of Dermatology, 2010Co-Authors: Amy Basile, Soheil Simzar, Jason J Bentow, Fernando Antelo, Paul K Shitabata, Shikaung Peng, Noah CraftAbstract:Hyperinfection caused by Strongyloides stercoralis in iatrogenically immunosuppressed patients is becoming more frequently observed. Here, we review the relevant literature and present a recent case of hyperinfection syndrome of S stercoralis in a patient chronically treated with systemic corticosteroids and methotrexate for dermatomyositis. The patient was born in Guatemala but no history of Strongyloides infection was documented. Disseminated Strongyloides is often associated with the immunocompromised state and is commonly seen with cutaneous lesions, respiratory failure, and sepsis. In this patient, a protracted course of progressive muscle weakness and multiple hospital stays for respiratory distress led to acute respiratory failure, septic shock, and rapid physical decline. A few days preceding his death, the patient developed petechiae and multiple purpuric macules and patches over the abdomen and thighs. Histologic review of skin biopsy specimens demonstrated multiple intravascular and interstitial filariform larvae. Dermatologists should be aware of patient populations at risk for infection with S stercoralis and be able to make this diagnosis to initiate earlier treatment of hyperinfection and dissemination.
