The Experts below are selected from a list of 2241 Experts worldwide ranked by ideXlab platform
D L Tanelian - One of the best experts on this subject based on the ideXlab platform.
-
Free Nerve Ending terminal morphology is fiber type specific for a delta and c fibers innervating rabbit corneal epithelium
Journal of Neurophysiology, 1993Co-Authors: M B Maciver, D L TanelianAbstract:1. A delta and C fibers are the smallest diameter and most numerous axons in peripheral Nerve bundles. They have been thought to terminate as "Free" Nerve Endings lacking organized structure. The p...
-
Free Nerve Ending terminal morphology is fiber type specific for A delta and C fibers innervating rabbit corneal epithelium
Journal of Neurophysiology, 1993Co-Authors: M B Maciver, D L TanelianAbstract:1. A delta and C fibers are the smallest diameter and most numerous axons in peripheral Nerve bundles. They have been thought to terminate as "Free" Nerve Endings lacking organized structure. The present study used a vital fluorescent dye to selectively visualize living Free Nerve Endings innervating rabbit corneal epithelium, allowing structure to be correlated with electrophysiological and functional characteristics. 2. Conduction velocity measurement of visually identified Nerve Endings were used to discriminate between C and A delta fibers. C fiber sensory Endings terminated as short (< 50 microns) vertically directed processes clustered within the epithelium. A delta fibers terminated as long (0.1-1.2 mm) horizontal processes running parallel to the epithelial surface. 3. Only A delta fiber Endings were mechanoreceptive, and the unique elongated structure imparted directional selectivity. Comparison of physiological and electrical activation indicated that mechanical stimuli were transduced in < 600 microseconds. This study confirms previous suggestions of structural and functional specialization for "Free" Nerve Endings.
M B Maciver - One of the best experts on this subject based on the ideXlab platform.
-
Free Nerve Ending terminal morphology is fiber type specific for a delta and c fibers innervating rabbit corneal epithelium
Journal of Neurophysiology, 1993Co-Authors: M B Maciver, D L TanelianAbstract:1. A delta and C fibers are the smallest diameter and most numerous axons in peripheral Nerve bundles. They have been thought to terminate as "Free" Nerve Endings lacking organized structure. The p...
-
Free Nerve Ending terminal morphology is fiber type specific for A delta and C fibers innervating rabbit corneal epithelium
Journal of Neurophysiology, 1993Co-Authors: M B Maciver, D L TanelianAbstract:1. A delta and C fibers are the smallest diameter and most numerous axons in peripheral Nerve bundles. They have been thought to terminate as "Free" Nerve Endings lacking organized structure. The present study used a vital fluorescent dye to selectively visualize living Free Nerve Endings innervating rabbit corneal epithelium, allowing structure to be correlated with electrophysiological and functional characteristics. 2. Conduction velocity measurement of visually identified Nerve Endings were used to discriminate between C and A delta fibers. C fiber sensory Endings terminated as short (< 50 microns) vertically directed processes clustered within the epithelium. A delta fibers terminated as long (0.1-1.2 mm) horizontal processes running parallel to the epithelial surface. 3. Only A delta fiber Endings were mechanoreceptive, and the unique elongated structure imparted directional selectivity. Comparison of physiological and electrical activation indicated that mechanical stimuli were transduced in < 600 microseconds. This study confirms previous suggestions of structural and functional specialization for "Free" Nerve Endings.
Bruce M Maciver - One of the best experts on this subject based on the ideXlab platform.
-
simultaneous visualization and electrophysiology of corneal a delta and c fiber afferents
Journal of Neuroscience Methods, 1990Co-Authors: Darrell L Tanelian, Bruce M MaciverAbstract:Abstract Fluorescent staining of neuronal elements has become an important tool in neuroscience research; however, investigations have been limited by the toxic effects produced by most dyes, especially following excitation by strong epifluorescent illumination. The present study investigated effects produced by the methylpyridinium fluorescent dyes, 4-di-1-ASP and 4-di-2-ASP, on electrophysiologic responses from corneal A-delta and C fiber afferents. Rabbit corneal tissue was isolated and maintained in vitro to facilitate staining, visualization, and electrophysiologic recording of corneal Nerves. Nerve fibers were selectively stained by the dyes and could be followed from their point of entry in small Nerve bundles at the cornea-sclera border to individual Free Nerve Ending terminals in the corneal epithelium. Neither the dyes nor epifluorescence, alone or in combination, produced any statistically significant changes ( P > 0.1; t test) in spontaneous discharge activity, spike amplitude, spike d V /d t or stimulus evoked activity in A-delta and C fibers. Epifluorescent visualization of corneal afferents and simultaneous electrophysiologic recording will permit detailed investigations of sensory transduction processes and structure-function relationships in mammalian sensory Nerves.
Darrell L Tanelian - One of the best experts on this subject based on the ideXlab platform.
-
simultaneous visualization and electrophysiology of corneal a delta and c fiber afferents
Journal of Neuroscience Methods, 1990Co-Authors: Darrell L Tanelian, Bruce M MaciverAbstract:Abstract Fluorescent staining of neuronal elements has become an important tool in neuroscience research; however, investigations have been limited by the toxic effects produced by most dyes, especially following excitation by strong epifluorescent illumination. The present study investigated effects produced by the methylpyridinium fluorescent dyes, 4-di-1-ASP and 4-di-2-ASP, on electrophysiologic responses from corneal A-delta and C fiber afferents. Rabbit corneal tissue was isolated and maintained in vitro to facilitate staining, visualization, and electrophysiologic recording of corneal Nerves. Nerve fibers were selectively stained by the dyes and could be followed from their point of entry in small Nerve bundles at the cornea-sclera border to individual Free Nerve Ending terminals in the corneal epithelium. Neither the dyes nor epifluorescence, alone or in combination, produced any statistically significant changes ( P > 0.1; t test) in spontaneous discharge activity, spike amplitude, spike d V /d t or stimulus evoked activity in A-delta and C fibers. Epifluorescent visualization of corneal afferents and simultaneous electrophysiologic recording will permit detailed investigations of sensory transduction processes and structure-function relationships in mammalian sensory Nerves.
Aziz Moqrich - One of the best experts on this subject based on the ideXlab platform.
-
Genetic ablation of GINIP-expressing primary sensory neurons strongly impairs Formalin-evoked pain
Scientific Reports, 2017Co-Authors: Louise Urien, Stéphane Gaillard, Pascale Malapert, Manon Bohic, Ana Reynders, Aziz MoqrichAbstract:Primary sensory neurons are heterogeneous by myriad of molecular criteria. However, the functional significance of this remarkable heterogeneity is just emerging. We precedently described the GINIP+ neurons as a new subpopulation of non peptidergic C-fibers encompassing the Free Nerve Ending cutaneous MRGPRD+ neurons and C-LTMRs. Using our recently generated ginip mouse model, we have been able to selectively ablate the GINIP+ neurons and assess their functional role in the somatosensation. We found that ablation of GINIP+ neurons affected neither the molecular contents nor the central projections of the spared neurons. GINIP-DTR mice exhibited impaired sensation to gentle mechanical stimuli applied to their hairy skin and had normal responses to noxious mechanical stimuli applied to their glabrous skin, under acute and injury-induced conditions. Importantly, loss of GINIP+ neurons significantly altered formalin-evoked first pain and drastically suppressed the second pain response. Given that MRGPRD+ neurons have been shown to be dispensable for formalin-evoked pain, our study suggest that C-LTMRs play a critical role in the modulation of formalin-evoked pain.
