The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Peter Šalamún - One of the best experts on this subject based on the ideXlab platform.
-
Perch and Its Parasites as Heavy Metal Biomonitors in a Freshwater Environment: The Case Study of the Ružín Water Reservoir, Slovakia
Sensors, 2012Co-Authors: Timea Brázová, Catarina Eira, Vladimíra Hanzelová, Jordi Torres, Dana Miklisová, Peter ŠalamúnAbstract:Heavy metal concentrations were determined in 43 perches (Perca fluviatilis) and in two of its most common parasites, the acanthocephalan Acanthocephalus lucii and the cestode Proteocephalus percae, collected in the period 2009–2010 from Ružin, a seriously polluted water reservoir in Slovakia. Samples of muscle, liver, kidney, brain, male and female reproductive organs and adipose tissue of fish and both parasites were analyzed for As, Cd, Cr, Cu, Hg, Mn, Ni, Pb and Zn, by ICP-MS. Mean concentrations of individual heavy metals in all fish samples decreased in the order zinc > copper > manganese > mercury > arsenic > chromium > cadmium > nickel > lead. Zinc was found to be the dominant element and its antagonistic interaction with copper was confirmed. The kidney was a key target organ receiving the highest mean concentrations of all analyzed metals, but some metals showed specific affinity for particular tissues. In terms of human health, concentration of Hg in fish muscle, which exceeded more than two-times its maximum level admitted in foodstuffs in European countries, is of great importance and should be taken into account. Bioaccumulation factors (C[parasite]/C[fish tissue]) calculated for all elements indicated much higher detection skills of A. lucii and P. percae parasites than fish organs and hence, present results allow proposing both parasite models as useful tools to monitor aquatic Environmental quality. Acanthocephalans, however, seem to be superior for heavy metal monitoring, also demonstrated under experimental conditions. Present results also indicate the decreasing heavy metal burden of the reservoir and its gradual recovery in the course of time.
Rakpong Petkam - One of the best experts on this subject based on the ideXlab platform.
-
Toxicity of Neem Leaf Extracts (Azadirachta indica A. Juss) on Some Haematological, Ionoregulatory, Biochemical and Enzymological Parameters of Indian Major Carp, Cirrhinus mrigala
2016Co-Authors: Manoharan Saravanan, Mathan Ramesh, Annamalai Malarvizhi, Rakpong PetkamAbstract:In the present study, the median lethal concentration (LC 50) of neem leaf extract to Cirrhinus mrigala for 24 h was found to be 1.035 g l-l. During the study period, the haematological parameters including Hb, Hct, RBC, MCV, MCH and MCHC levels were significantly decreased in neem leaf extract exposed fish when compared to the control fish whereas WBC count was increased. Similarly, plasma Na+ and Cl- levels were significantly lower and K+ level were significantly higher when compared to the control. In biochemical study, elevated plasma glucose and induced protein levels were noticed. The enzymes, glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) activities were increased significantly in gill, liver and muscle of treated fish compared to that of their control groups. The results of the present investigation suggest that neem leaf extracts affects the hematological, ionoregulatory, biochemical and enzymological parameters of fish and alterations of these parameters can be useful in Environmental biomonitoring of neem based products in Freshwater Environment
-
toxicity of neem leaf extracts azadirachta indica a juss on some haematological ionoregulatory biochemical and enzymological parameters of indian major carp cirrhinus mrigala
Journal of Tropical Forestry, 2011Co-Authors: Manoharan Saravanan, Mathan Ramesh, Annamalai Malarvizhi, Rakpong PetkamAbstract:In the present study, the median lethal concentration (LC 50) of neem leaf extract to Cirrhinus mrigala for 24 h was found to be 1.035 g l-l. During the study period, the haematological parameters including Hb, Hct, RBC, MCV, MCH and MCHC levels were significantly decreased in neem leaf extract exposed fish when compared to the control fish whereas WBC count was increased. Similarly, plasma Na+ and Cl- levels were significantly lower and K+ level were significantly higher when compared to the control. In biochemical study, elevated plasma glucose and induced protein levels were noticed. The enzymes, glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) activities were increased significantly in gill, liver and muscle of treated fish compared to that of their control groups. The results of the present investigation suggest that neem leaf extracts affects the hematological, ionoregulatory, biochemical and enzymological parameters of fish and alterations of these parameters can be useful in Environmental biomonitoring of neem based products in Freshwater Environment. Keywords: Azadirachta indica, Acute toxicity, Cirrhinus mrigala, Haematology, Ion regulation, Biochemical and Enzymological parameters.
Herve Pouliquen - One of the best experts on this subject based on the ideXlab platform.
-
The bryophyte Fontinalis antipyretica Hedw. bioaccumulates oxytetracycline, flumequine and oxolinic acid in the Freshwater Environment
Science of the Total Environment, 2004Co-Authors: Raphael Delepee, Herve Pouliquen, Hervé Le BrisAbstract:In recent years, the fate of pharmacological substances in the aquatic Environment have been more and more studied. Oxolinic acid (OA), flumequine (FLU) and oxytetracycline (OTC) are commonly used antibacterial agents. A large amount of these drugs is released into water directly by dissolved fraction and indirectly in urine and feces. Monitoring these compounds in the Freshwater Environment is difficult because of the lack of suitable indicators. The aim of this work was to evaluate the OA, FLU and OTC bioaccumulation abilities of Fontinalis antipyretica Hedw., known for heavy metal bioaccumulation. The experiment described was decomposed for two times: a 10-days accumulation period during which bryophytes were in contact with antibiotics and a 15-days post-exposure period during which bryophytes were in water with no antibiotic. This experiment showed that this bryophyte strongly accumulates OA, FLU and OTC in Freshwater. Bioaccumulation factors (ratio of concentrations in bryophyte and water) ranged between 75 and 450. Moreover, OA, FLU and OTC persisted in the bryophyte for a long time with clearance between 0.19 and 3.04 ng/g/day. Mean residence times ranged between 18 and 59 days. Accumulation and decontamination mechanism models were proposed
-
ion paired solid phase extraction as a sample preparation strategy for the high performance liquid chromatographic determination of oxytetracycline in the bryophyte fontinalis antipyretica
Analytica Chimica Acta, 2003Co-Authors: Raphael Delepee, Herve PouliquenAbstract:Oxytetracycline (OTC) is one of the major antibacterial agent used in Freshwater fish farming. Because of its rapid decomposition rate in sediments, it is difficult to monitor OTC in the Freshwater Environment. Fontinalis antipyreticais known for its bioaccumulation properties. Determining OTC concentration in F. antipyretica by HPLC was a real challenge because pigments coextracted with OTC. Ion-pairing was used in the clean-up procedure to improve the selectivity of the C18 solid phase extraction. Octanesulfonic acid was used as counter-ion. Tetracycline was used as internal standard. The linearity ranged between 100 and 1000 ng/g and limits of detection and quantitation were 30 and 100 ng/g, respectively. Mean recovery for OTC was 48.2% and the validation procedure demonstrated that the method was selective, reliable and precise enough to allow Environmental monitoring. This method was applied to a 25 days experimental study showing the bioaccumulation of OTC by the bryophyte F. antipyretica. © 2002 Elsevier Science B.V. All rights reserved.
Raphael Delepee - One of the best experts on this subject based on the ideXlab platform.
-
The bryophyte Fontinalis antipyretica Hedw. bioaccumulates oxytetracycline, flumequine and oxolinic acid in the Freshwater Environment
Science of the Total Environment, 2004Co-Authors: Raphael Delepee, Herve Pouliquen, Hervé Le BrisAbstract:In recent years, the fate of pharmacological substances in the aquatic Environment have been more and more studied. Oxolinic acid (OA), flumequine (FLU) and oxytetracycline (OTC) are commonly used antibacterial agents. A large amount of these drugs is released into water directly by dissolved fraction and indirectly in urine and feces. Monitoring these compounds in the Freshwater Environment is difficult because of the lack of suitable indicators. The aim of this work was to evaluate the OA, FLU and OTC bioaccumulation abilities of Fontinalis antipyretica Hedw., known for heavy metal bioaccumulation. The experiment described was decomposed for two times: a 10-days accumulation period during which bryophytes were in contact with antibiotics and a 15-days post-exposure period during which bryophytes were in water with no antibiotic. This experiment showed that this bryophyte strongly accumulates OA, FLU and OTC in Freshwater. Bioaccumulation factors (ratio of concentrations in bryophyte and water) ranged between 75 and 450. Moreover, OA, FLU and OTC persisted in the bryophyte for a long time with clearance between 0.19 and 3.04 ng/g/day. Mean residence times ranged between 18 and 59 days. Accumulation and decontamination mechanism models were proposed
-
ion paired solid phase extraction as a sample preparation strategy for the high performance liquid chromatographic determination of oxytetracycline in the bryophyte fontinalis antipyretica
Analytica Chimica Acta, 2003Co-Authors: Raphael Delepee, Herve PouliquenAbstract:Oxytetracycline (OTC) is one of the major antibacterial agent used in Freshwater fish farming. Because of its rapid decomposition rate in sediments, it is difficult to monitor OTC in the Freshwater Environment. Fontinalis antipyreticais known for its bioaccumulation properties. Determining OTC concentration in F. antipyretica by HPLC was a real challenge because pigments coextracted with OTC. Ion-pairing was used in the clean-up procedure to improve the selectivity of the C18 solid phase extraction. Octanesulfonic acid was used as counter-ion. Tetracycline was used as internal standard. The linearity ranged between 100 and 1000 ng/g and limits of detection and quantitation were 30 and 100 ng/g, respectively. Mean recovery for OTC was 48.2% and the validation procedure demonstrated that the method was selective, reliable and precise enough to allow Environmental monitoring. This method was applied to a 25 days experimental study showing the bioaccumulation of OTC by the bryophyte F. antipyretica. © 2002 Elsevier Science B.V. All rights reserved.
Timea Brázová - One of the best experts on this subject based on the ideXlab platform.
-
Perch and Its Parasites as Heavy Metal Biomonitors in a Freshwater Environment: The Case Study of the Ružín Water Reservoir, Slovakia
Sensors, 2012Co-Authors: Timea Brázová, Catarina Eira, Vladimíra Hanzelová, Jordi Torres, Dana Miklisová, Peter ŠalamúnAbstract:Heavy metal concentrations were determined in 43 perches (Perca fluviatilis) and in two of its most common parasites, the acanthocephalan Acanthocephalus lucii and the cestode Proteocephalus percae, collected in the period 2009–2010 from Ružin, a seriously polluted water reservoir in Slovakia. Samples of muscle, liver, kidney, brain, male and female reproductive organs and adipose tissue of fish and both parasites were analyzed for As, Cd, Cr, Cu, Hg, Mn, Ni, Pb and Zn, by ICP-MS. Mean concentrations of individual heavy metals in all fish samples decreased in the order zinc > copper > manganese > mercury > arsenic > chromium > cadmium > nickel > lead. Zinc was found to be the dominant element and its antagonistic interaction with copper was confirmed. The kidney was a key target organ receiving the highest mean concentrations of all analyzed metals, but some metals showed specific affinity for particular tissues. In terms of human health, concentration of Hg in fish muscle, which exceeded more than two-times its maximum level admitted in foodstuffs in European countries, is of great importance and should be taken into account. Bioaccumulation factors (C[parasite]/C[fish tissue]) calculated for all elements indicated much higher detection skills of A. lucii and P. percae parasites than fish organs and hence, present results allow proposing both parasite models as useful tools to monitor aquatic Environmental quality. Acanthocephalans, however, seem to be superior for heavy metal monitoring, also demonstrated under experimental conditions. Present results also indicate the decreasing heavy metal burden of the reservoir and its gradual recovery in the course of time.
