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L. A. Caicedo - One of the best experts on this subject based on the ideXlab platform.
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Semibatch and continuous Fructooligosaccharides production by Aspergillus sp. N74 in a mechanically agitated airlift reactor
Journal of Chemical Technology & Biotechnology, 2009Co-Authors: L. A. Caicedo, Edelberto Silva, Oscar F. SánchezAbstract:BACKGROUND: Fructooligosaccharides are important sweeteners produced by sucrose biotransformation. Although fructooligosccharides production has been reported widely, most studies have been carried out at laboratory level. This study evaluates semibatch and continuous Fructooligosaccharides production by Aspergillus sp. N74 at bench scale in a mechanically agitated airlift. RESULTS: Sucrose biotransformation to Fructooligosaccharides was carried out with biomass harvested after 24 or 48 h of culture. For 6.21 ± 0.33 or 9.66 ± 0.62 g biomass dry weight L−1, the highest FOS yields were obtained at batch operating 62.1 and 66.4% after 26 or 6 h of reaction, respectively. Reduction in Fructooligosaccharides yield was observed for both biomass concentrations at semibatch operating, while a comparable yield was obtained during continuous operating (62.1% for 6.21 ± 0.33 g L−1 and a dilution rate 0.016 s−1, and 62.8% for 9.66 ± 0.62 g L−1 and a dilution rate 0.032 s−1). Nevertheless, 1-kestose formation was favored with biomass harvested after 24 h under any operating mode. CONCLUSION: Biomass concentration, reaction time and operating mode have a notable effect on Fructooligosaccharides yield and composition. 1-kestose, the most valuable Fructooligosaccharide, was obtained in greatest proportion at a biomass concentration 6.21 ± 0.33 g L−1. Under the different operating modes, Aspergillus sp. N74 mycelia and the reactor described are presented as a feasible alternative for scaling up Fructooligosaccharides production. Copyright © 2008 Society of Chemical Industry
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Sucrose Biotransformation to Fructooligosaccharides by Aspergillus sp. N74 Free Cells
Food and Bioprocess Technology, 2008Co-Authors: Oscar F. Sánchez, Edelberto Silva, Ana M. Rodríguez, L. A. CaicedoAbstract:Fructooligosaccharide production with the fructosyltransferase from free cells of the native strain Aspergillus sp. N74 at laboratory level was evaluated. The biomass of the native strain Aspergillus sp. N74 was produced in a sucrose fermentation medium and was employed in the enzymatic reaction in solutions of sucrose and phosphate buffer, where pH, temperature, and initial sucrose concentration effect were evaluated. Fructooligosaccharides and reaction subproducts were identified and quantified by high-performance liquid chromatography. The enzyme produced by the strain Aspergillus sp. N74 possessed hydrolytic and transfructosylating activities that changed with process conditions. The best transfructosylating condition was obtained at 80 min reaction time at pH 5.5, 60 °C, and initial sucrose concentrations higher than 550 g L−1, with Fructooligosaccharide production of about 50% w/w (based on initial sucrose concentration) and conversion selectivity higher than 90%. In addition, the transfructosylating and hydrolytic activities ratio was of 20. The high transfructosylating activity showed by the fructosyltransferase produced from the native strain Aspergillus sp. N74 suggest considering it as an alternative for the scale-up production of Fructooligosaccharides by means of the whole microorganism at bench and pilot plant levels.
Oscar F. Sánchez - One of the best experts on this subject based on the ideXlab platform.
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Use of spent osmotic solutions for the production of Fructooligosaccharides by Aspergillus oryzae N74
Food science and technology international = Ciencia y tecnologia de los alimentos internacional, 2013Co-Authors: Y. Ruiz, Bernadette Klotz, Juan Carlos Serrato, Felipe Guio, Jorge Bohorquez, Oscar F. SánchezAbstract:In the food industry, osmotic dehydration can be an important stage to obtain partially dry foodstuffs. However, the remaining spent osmotic solution at the end of the process could become a waste with an important environmental impact due to the large amount of organic compounds that it might contain. Since one of the most important osmotic agents used in osmotic dehydration is sucrose, this spent osmotic solution could be used to be biotransformed to produce Fructooligosaccharides by a fructosyltransferase. This study evaluated the production of Fructooligosaccharides using the fructosyltransferase produced by Aspergillus oryzae N74, and the spent osmotic solution that resulted in the osmotic dehydration of Andes berry (Rubus glaucus) and tamarillo (Cyphomandra betacea). Assays were conducted at small and bioreactor scales, using spent osmotic solution with or without re-concentration. At small scale no significant difference (p > 0.05) was observed in the Fructooligosaccharides production yield, ranging from 31.18% to 34.98% for spent osmotic solution from tamarillo osmotic dehydration, and from 33.16% to 37.52% for spent osmotic solution from Andes berry osmotic dehydration, using either the SOS with or without re-concentration. At bioreactor scale the highest Fructooligosaccharides yield of 58.51 ± 1.73% was obtained with spent osmotic solution that resulted from tamarillo osmotic dehydration. With the spent osmotic solution from Andes berry osmotic dehydration the yield was 49.17 ± 2.82%. These results showed the feasibility of producing Fructooligosaccharides from spent osmotic solution that is considered a waste in food industry.
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Semibatch and continuous Fructooligosaccharides production by Aspergillus sp. N74 in a mechanically agitated airlift reactor
Journal of Chemical Technology & Biotechnology, 2009Co-Authors: L. A. Caicedo, Edelberto Silva, Oscar F. SánchezAbstract:BACKGROUND: Fructooligosaccharides are important sweeteners produced by sucrose biotransformation. Although fructooligosccharides production has been reported widely, most studies have been carried out at laboratory level. This study evaluates semibatch and continuous Fructooligosaccharides production by Aspergillus sp. N74 at bench scale in a mechanically agitated airlift. RESULTS: Sucrose biotransformation to Fructooligosaccharides was carried out with biomass harvested after 24 or 48 h of culture. For 6.21 ± 0.33 or 9.66 ± 0.62 g biomass dry weight L−1, the highest FOS yields were obtained at batch operating 62.1 and 66.4% after 26 or 6 h of reaction, respectively. Reduction in Fructooligosaccharides yield was observed for both biomass concentrations at semibatch operating, while a comparable yield was obtained during continuous operating (62.1% for 6.21 ± 0.33 g L−1 and a dilution rate 0.016 s−1, and 62.8% for 9.66 ± 0.62 g L−1 and a dilution rate 0.032 s−1). Nevertheless, 1-kestose formation was favored with biomass harvested after 24 h under any operating mode. CONCLUSION: Biomass concentration, reaction time and operating mode have a notable effect on Fructooligosaccharides yield and composition. 1-kestose, the most valuable Fructooligosaccharide, was obtained in greatest proportion at a biomass concentration 6.21 ± 0.33 g L−1. Under the different operating modes, Aspergillus sp. N74 mycelia and the reactor described are presented as a feasible alternative for scaling up Fructooligosaccharides production. Copyright © 2008 Society of Chemical Industry
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Sucrose Biotransformation to Fructooligosaccharides by Aspergillus sp. N74 Free Cells
Food and Bioprocess Technology, 2008Co-Authors: Oscar F. Sánchez, Edelberto Silva, Ana M. Rodríguez, L. A. CaicedoAbstract:Fructooligosaccharide production with the fructosyltransferase from free cells of the native strain Aspergillus sp. N74 at laboratory level was evaluated. The biomass of the native strain Aspergillus sp. N74 was produced in a sucrose fermentation medium and was employed in the enzymatic reaction in solutions of sucrose and phosphate buffer, where pH, temperature, and initial sucrose concentration effect were evaluated. Fructooligosaccharides and reaction subproducts were identified and quantified by high-performance liquid chromatography. The enzyme produced by the strain Aspergillus sp. N74 possessed hydrolytic and transfructosylating activities that changed with process conditions. The best transfructosylating condition was obtained at 80 min reaction time at pH 5.5, 60 °C, and initial sucrose concentrations higher than 550 g L−1, with Fructooligosaccharide production of about 50% w/w (based on initial sucrose concentration) and conversion selectivity higher than 90%. In addition, the transfructosylating and hydrolytic activities ratio was of 20. The high transfructosylating activity showed by the fructosyltransferase produced from the native strain Aspergillus sp. N74 suggest considering it as an alternative for the scale-up production of Fructooligosaccharides by means of the whole microorganism at bench and pilot plant levels.
Francisco Maugeri Filho - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of Fructooligosaccharides separation using a fixed-bed column packed with activated charcoal.
New biotechnology, 2014Co-Authors: Raquel C. Kuhn, Marcio A. Mazutti, Lilian Buoro Albertini, Francisco Maugeri FilhoAbstract:Recent studies have shown that the chromatographic separation of mixtures of saccharides may be improved by making use of activated charcoal, a promising low cost material for the separation of sugars, including Fructooligosaccharides. In this work, the development of a methodology to separate Fructooligosaccharides from glucose, fructose and sucrose, using a fixed bed column packed with activated charcoal is proposed. The influence of temperature, eluant concentration and step gradients were evaluated to increase the separation efficiency and Fructooligosaccharide purity. The final degree of Fructooligosaccharide purification and separation efficiency were about 94% and 3.03 respectively, using ethanol gradient concentration ranging from 3.5% to 15% (v/v) at 40°C. The fixed bed column packed with the activated charcoal was shown to be a promising alternative for sugar separation, mainly those rich in Fructooligosaccharides, leading to solutions of acceptable degrees of purification.
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Purification of Fructooligosaccharides in an activated charcoal fixed bed column.
New biotechnology, 2010Co-Authors: Raquel C. Kuhn, Francisco Maugeri FilhoAbstract:Fructooligosaccharides (FOS) are mixtures of oligosaccharides containing mono and disaccharides, therefore, the separation of these sugars results in purer products for human consumption and can be added to various food products (drinks, cookies and yogurt). The aim of this work was the purification of Fructooligosaccharides from a mixture of sugars, obtained by enzymatic synthesis, containing Fructooligosaccharides, glucose, fructose and sucrose using activated charcoal fixed bed column. Temperature and ethanol concentration effects were analyzed using a 22 central composite design. Good separation conditions were obtained through central composite design. The best separation coefficient between Fructooligosaccharides and glucose (ESfructoolig/gluc) was 3.99 ± 0.07 and between fructose and Fructooligosaccharides (ESfructoolig/fruct) was 2.89 ± 0.36 using ethanol 15% (v/v) as eluent, at 50 °C. The final FOS purification degree and recovery were about 80% and 97.8%, respectively. The activated charcoal fixed bed columns were shown to be a good alternative for sugar separation, especially for rich mixtures of Fructooligosaccharides.
Edelberto Silva - One of the best experts on this subject based on the ideXlab platform.
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Semibatch and continuous Fructooligosaccharides production by Aspergillus sp. N74 in a mechanically agitated airlift reactor
Journal of Chemical Technology & Biotechnology, 2009Co-Authors: L. A. Caicedo, Edelberto Silva, Oscar F. SánchezAbstract:BACKGROUND: Fructooligosaccharides are important sweeteners produced by sucrose biotransformation. Although fructooligosccharides production has been reported widely, most studies have been carried out at laboratory level. This study evaluates semibatch and continuous Fructooligosaccharides production by Aspergillus sp. N74 at bench scale in a mechanically agitated airlift. RESULTS: Sucrose biotransformation to Fructooligosaccharides was carried out with biomass harvested after 24 or 48 h of culture. For 6.21 ± 0.33 or 9.66 ± 0.62 g biomass dry weight L−1, the highest FOS yields were obtained at batch operating 62.1 and 66.4% after 26 or 6 h of reaction, respectively. Reduction in Fructooligosaccharides yield was observed for both biomass concentrations at semibatch operating, while a comparable yield was obtained during continuous operating (62.1% for 6.21 ± 0.33 g L−1 and a dilution rate 0.016 s−1, and 62.8% for 9.66 ± 0.62 g L−1 and a dilution rate 0.032 s−1). Nevertheless, 1-kestose formation was favored with biomass harvested after 24 h under any operating mode. CONCLUSION: Biomass concentration, reaction time and operating mode have a notable effect on Fructooligosaccharides yield and composition. 1-kestose, the most valuable Fructooligosaccharide, was obtained in greatest proportion at a biomass concentration 6.21 ± 0.33 g L−1. Under the different operating modes, Aspergillus sp. N74 mycelia and the reactor described are presented as a feasible alternative for scaling up Fructooligosaccharides production. Copyright © 2008 Society of Chemical Industry
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Sucrose Biotransformation to Fructooligosaccharides by Aspergillus sp. N74 Free Cells
Food and Bioprocess Technology, 2008Co-Authors: Oscar F. Sánchez, Edelberto Silva, Ana M. Rodríguez, L. A. CaicedoAbstract:Fructooligosaccharide production with the fructosyltransferase from free cells of the native strain Aspergillus sp. N74 at laboratory level was evaluated. The biomass of the native strain Aspergillus sp. N74 was produced in a sucrose fermentation medium and was employed in the enzymatic reaction in solutions of sucrose and phosphate buffer, where pH, temperature, and initial sucrose concentration effect were evaluated. Fructooligosaccharides and reaction subproducts were identified and quantified by high-performance liquid chromatography. The enzyme produced by the strain Aspergillus sp. N74 possessed hydrolytic and transfructosylating activities that changed with process conditions. The best transfructosylating condition was obtained at 80 min reaction time at pH 5.5, 60 °C, and initial sucrose concentrations higher than 550 g L−1, with Fructooligosaccharide production of about 50% w/w (based on initial sucrose concentration) and conversion selectivity higher than 90%. In addition, the transfructosylating and hydrolytic activities ratio was of 20. The high transfructosylating activity showed by the fructosyltransferase produced from the native strain Aspergillus sp. N74 suggest considering it as an alternative for the scale-up production of Fructooligosaccharides by means of the whole microorganism at bench and pilot plant levels.
Raquel C. Kuhn - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of Fructooligosaccharides separation using a fixed-bed column packed with activated charcoal.
New biotechnology, 2014Co-Authors: Raquel C. Kuhn, Marcio A. Mazutti, Lilian Buoro Albertini, Francisco Maugeri FilhoAbstract:Recent studies have shown that the chromatographic separation of mixtures of saccharides may be improved by making use of activated charcoal, a promising low cost material for the separation of sugars, including Fructooligosaccharides. In this work, the development of a methodology to separate Fructooligosaccharides from glucose, fructose and sucrose, using a fixed bed column packed with activated charcoal is proposed. The influence of temperature, eluant concentration and step gradients were evaluated to increase the separation efficiency and Fructooligosaccharide purity. The final degree of Fructooligosaccharide purification and separation efficiency were about 94% and 3.03 respectively, using ethanol gradient concentration ranging from 3.5% to 15% (v/v) at 40°C. The fixed bed column packed with the activated charcoal was shown to be a promising alternative for sugar separation, mainly those rich in Fructooligosaccharides, leading to solutions of acceptable degrees of purification.
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Purification of Fructooligosaccharides in an activated charcoal fixed bed column.
New biotechnology, 2010Co-Authors: Raquel C. Kuhn, Francisco Maugeri FilhoAbstract:Fructooligosaccharides (FOS) are mixtures of oligosaccharides containing mono and disaccharides, therefore, the separation of these sugars results in purer products for human consumption and can be added to various food products (drinks, cookies and yogurt). The aim of this work was the purification of Fructooligosaccharides from a mixture of sugars, obtained by enzymatic synthesis, containing Fructooligosaccharides, glucose, fructose and sucrose using activated charcoal fixed bed column. Temperature and ethanol concentration effects were analyzed using a 22 central composite design. Good separation conditions were obtained through central composite design. The best separation coefficient between Fructooligosaccharides and glucose (ESfructoolig/gluc) was 3.99 ± 0.07 and between fructose and Fructooligosaccharides (ESfructoolig/fruct) was 2.89 ± 0.36 using ethanol 15% (v/v) as eluent, at 50 °C. The final FOS purification degree and recovery were about 80% and 97.8%, respectively. The activated charcoal fixed bed columns were shown to be a good alternative for sugar separation, especially for rich mixtures of Fructooligosaccharides.
