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L. A. Caicedo - One of the best experts on this subject based on the ideXlab platform.
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Semibatch and continuous Fructooligosaccharides production by Aspergillus sp. N74 in a mechanically agitated airlift reactor
Journal of Chemical Technology & Biotechnology, 2009Co-Authors: L. A. Caicedo, Edelberto Silva, Oscar F. SánchezAbstract:BACKGROUND: Fructooligosaccharides are important sweeteners produced by sucrose biotransformation. Although fructooligosccharides production has been reported widely, most studies have been carried out at laboratory level. This study evaluates semibatch and continuous Fructooligosaccharides production by Aspergillus sp. N74 at bench scale in a mechanically agitated airlift. RESULTS: Sucrose biotransformation to Fructooligosaccharides was carried out with biomass harvested after 24 or 48 h of culture. For 6.21 ± 0.33 or 9.66 ± 0.62 g biomass dry weight L−1, the highest FOS yields were obtained at batch operating 62.1 and 66.4% after 26 or 6 h of reaction, respectively. Reduction in Fructooligosaccharides yield was observed for both biomass concentrations at semibatch operating, while a comparable yield was obtained during continuous operating (62.1% for 6.21 ± 0.33 g L−1 and a dilution rate 0.016 s−1, and 62.8% for 9.66 ± 0.62 g L−1 and a dilution rate 0.032 s−1). Nevertheless, 1-kestose formation was favored with biomass harvested after 24 h under any operating mode. CONCLUSION: Biomass concentration, reaction time and operating mode have a notable effect on Fructooligosaccharides yield and composition. 1-kestose, the most valuable fructooligosaccharide, was obtained in greatest proportion at a biomass concentration 6.21 ± 0.33 g L−1. Under the different operating modes, Aspergillus sp. N74 mycelia and the reactor described are presented as a feasible alternative for scaling up Fructooligosaccharides production. Copyright © 2008 Society of Chemical Industry
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Sucrose Biotransformation to Fructooligosaccharides by Aspergillus sp. N74 Free Cells
Food and Bioprocess Technology, 2008Co-Authors: Oscar F. Sánchez, Edelberto Silva, Ana M. Rodríguez, L. A. CaicedoAbstract:Fructooligosaccharide production with the fructosyltransferase from free cells of the native strain Aspergillus sp. N74 at laboratory level was evaluated. The biomass of the native strain Aspergillus sp. N74 was produced in a sucrose fermentation medium and was employed in the enzymatic reaction in solutions of sucrose and phosphate buffer, where pH, temperature, and initial sucrose concentration effect were evaluated. Fructooligosaccharides and reaction subproducts were identified and quantified by high-performance liquid chromatography. The enzyme produced by the strain Aspergillus sp. N74 possessed hydrolytic and transfructosylating activities that changed with process conditions. The best transfructosylating condition was obtained at 80 min reaction time at pH 5.5, 60 °C, and initial sucrose concentrations higher than 550 g L−1, with fructooligosaccharide production of about 50% w/w (based on initial sucrose concentration) and conversion selectivity higher than 90%. In addition, the transfructosylating and hydrolytic activities ratio was of 20. The high transfructosylating activity showed by the fructosyltransferase produced from the native strain Aspergillus sp. N74 suggest considering it as an alternative for the scale-up production of Fructooligosaccharides by means of the whole microorganism at bench and pilot plant levels.
Oscar F. Sánchez - One of the best experts on this subject based on the ideXlab platform.
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Use of spent osmotic solutions for the production of Fructooligosaccharides by Aspergillus oryzae N74
Food science and technology international = Ciencia y tecnologia de los alimentos internacional, 2013Co-Authors: Y. Ruiz, Bernadette Klotz, Juan Carlos Serrato, Felipe Guio, Jorge Bohorquez, Oscar F. SánchezAbstract:In the food industry, osmotic dehydration can be an important stage to obtain partially dry foodstuffs. However, the remaining spent osmotic solution at the end of the process could become a waste with an important environmental impact due to the large amount of organic compounds that it might contain. Since one of the most important osmotic agents used in osmotic dehydration is sucrose, this spent osmotic solution could be used to be biotransformed to produce Fructooligosaccharides by a fructosyltransferase. This study evaluated the production of Fructooligosaccharides using the fructosyltransferase produced by Aspergillus oryzae N74, and the spent osmotic solution that resulted in the osmotic dehydration of Andes berry (Rubus glaucus) and tamarillo (Cyphomandra betacea). Assays were conducted at small and bioreactor scales, using spent osmotic solution with or without re-concentration. At small scale no significant difference (p > 0.05) was observed in the Fructooligosaccharides production yield, ranging from 31.18% to 34.98% for spent osmotic solution from tamarillo osmotic dehydration, and from 33.16% to 37.52% for spent osmotic solution from Andes berry osmotic dehydration, using either the SOS with or without re-concentration. At bioreactor scale the highest Fructooligosaccharides yield of 58.51 ± 1.73% was obtained with spent osmotic solution that resulted from tamarillo osmotic dehydration. With the spent osmotic solution from Andes berry osmotic dehydration the yield was 49.17 ± 2.82%. These results showed the feasibility of producing Fructooligosaccharides from spent osmotic solution that is considered a waste in food industry.
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Semibatch and continuous Fructooligosaccharides production by Aspergillus sp. N74 in a mechanically agitated airlift reactor
Journal of Chemical Technology & Biotechnology, 2009Co-Authors: L. A. Caicedo, Edelberto Silva, Oscar F. SánchezAbstract:BACKGROUND: Fructooligosaccharides are important sweeteners produced by sucrose biotransformation. Although fructooligosccharides production has been reported widely, most studies have been carried out at laboratory level. This study evaluates semibatch and continuous Fructooligosaccharides production by Aspergillus sp. N74 at bench scale in a mechanically agitated airlift. RESULTS: Sucrose biotransformation to Fructooligosaccharides was carried out with biomass harvested after 24 or 48 h of culture. For 6.21 ± 0.33 or 9.66 ± 0.62 g biomass dry weight L−1, the highest FOS yields were obtained at batch operating 62.1 and 66.4% after 26 or 6 h of reaction, respectively. Reduction in Fructooligosaccharides yield was observed for both biomass concentrations at semibatch operating, while a comparable yield was obtained during continuous operating (62.1% for 6.21 ± 0.33 g L−1 and a dilution rate 0.016 s−1, and 62.8% for 9.66 ± 0.62 g L−1 and a dilution rate 0.032 s−1). Nevertheless, 1-kestose formation was favored with biomass harvested after 24 h under any operating mode. CONCLUSION: Biomass concentration, reaction time and operating mode have a notable effect on Fructooligosaccharides yield and composition. 1-kestose, the most valuable fructooligosaccharide, was obtained in greatest proportion at a biomass concentration 6.21 ± 0.33 g L−1. Under the different operating modes, Aspergillus sp. N74 mycelia and the reactor described are presented as a feasible alternative for scaling up Fructooligosaccharides production. Copyright © 2008 Society of Chemical Industry
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Sucrose Biotransformation to Fructooligosaccharides by Aspergillus sp. N74 Free Cells
Food and Bioprocess Technology, 2008Co-Authors: Oscar F. Sánchez, Edelberto Silva, Ana M. Rodríguez, L. A. CaicedoAbstract:Fructooligosaccharide production with the fructosyltransferase from free cells of the native strain Aspergillus sp. N74 at laboratory level was evaluated. The biomass of the native strain Aspergillus sp. N74 was produced in a sucrose fermentation medium and was employed in the enzymatic reaction in solutions of sucrose and phosphate buffer, where pH, temperature, and initial sucrose concentration effect were evaluated. Fructooligosaccharides and reaction subproducts were identified and quantified by high-performance liquid chromatography. The enzyme produced by the strain Aspergillus sp. N74 possessed hydrolytic and transfructosylating activities that changed with process conditions. The best transfructosylating condition was obtained at 80 min reaction time at pH 5.5, 60 °C, and initial sucrose concentrations higher than 550 g L−1, with fructooligosaccharide production of about 50% w/w (based on initial sucrose concentration) and conversion selectivity higher than 90%. In addition, the transfructosylating and hydrolytic activities ratio was of 20. The high transfructosylating activity showed by the fructosyltransferase produced from the native strain Aspergillus sp. N74 suggest considering it as an alternative for the scale-up production of Fructooligosaccharides by means of the whole microorganism at bench and pilot plant levels.
M S Alles - One of the best experts on this subject based on the ideXlab platform.
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consumption of Fructooligosaccharides does not favorably affect blood glucose and serum lipid concentrations in patients with type 2 diabetes
The American Journal of Clinical Nutrition, 1999Co-Authors: M S Alles, N M De Roos, J C Bakx, E H Van De Lisdonk, P L Zock, J G A J HautvastAbstract:Background: Fructooligosaccharides have been claimed to lower fasting glycemia and serum total cholesterol concentrations, possibly via effects of short-chain fatty acids produced during fermentation. Objective: We studied the effects of Fructooligosaccharides on blood glucose, serum lipids, and serum acetate in 20 patients with type 2 diabetes. Design: In a randomized, single-blind, crossover design, patients consumed either glucose as a placebo (4 g/d) or Fructooligosaccharides (15 g/d) for 20 d each. Average daily intakes of energy, macronutrients, and dietary fiber were similar with both treatments. Results: Compliance, expressed as the proportion of supplements not returned, was near 100% during both treatments. Fructooligosaccharides did not significantly affect fasting concentrations (mmol/L) of serum total cholesterol (95% CI: 20.07, 0.48), HDL cholesterol (20.04, 0.04), LDL cholesterol (20.06, 0.34), serum triacylglycerols ( 20.21, 0.44), serum free fatty acids (20.08, 0.04), serum acetate (20.01, 0.01), or blood glucose (20.37, 0.40). Conclusions: We conclude that 20 d of dietary supplementation with Fructooligosaccharides had no major effect on blood glucose, serum lipids, or serum acetate in patients with type 2 diabetes. This lack of effect was not due to changes in dietary intake, insufficient statistical power, or noncompliance of the patients. Am J Clin Nutr 1999;69:64‐9.
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Bacterial fermentation of Fructooligosaccharides and resistant starch in patients with an ileal pouch-anal anastomosis
The American journal of clinical nutrition, 1997Co-Authors: M S Alles, M.b. Katan, J. M. J. I. Salemans, K.m.j. Van Laere, M.j.w. Gerichhausen, M.j. Rozendaal, Fokko M. NagengastAbstract:Patients with large bowel disease may undergo ileal pouch-anal anastomosis, in which the colon is removed and part of the distal ileum is used to construct a pelvic reservoir. Competence of the ileal pouch to ferment carbohydrates is associated with the absence of pouchitis. However, the extent to which bacterial fermentation takes place and whether it is affected by diet are unclear. We investigated fermentation of two nondigestible carbohydrates, Fructooligosaccharides and resistant starch, in 15 healthy patients with an ileal pouch by using a placebo-controlled crossover design (with glucose as the placebo). Apparent fermentability of Fructooligosaccharides was 83%; that of resistant starch was 46%. Resistant starch increased fecal excretion of butyrate by 69% whereas Fructooligosaccharides reduced excretion of amino acid-derived isobutyrate by 94% and of isovalerate by 77%. Fructooligosaccharides also significantly increased fecal weight (651 compared with 541 g/d) and breath-hydrogen excretion (286 compared with 85 ppm x h). Bacterial fermentation of nondigestible carbohydrates in pouches takes place to an appreciable extent and in a substrate-specific manner. The relation between such fermentation and inflammation of the pouch (pouchitis) deserves study.
Fokko M. Nagengast - One of the best experts on this subject based on the ideXlab platform.
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Bacterial fermentation of Fructooligosaccharides and resistant starch in patients with an ileal pouch-anal anastomosis
The American journal of clinical nutrition, 1997Co-Authors: M S Alles, M.b. Katan, J. M. J. I. Salemans, K.m.j. Van Laere, M.j.w. Gerichhausen, M.j. Rozendaal, Fokko M. NagengastAbstract:Patients with large bowel disease may undergo ileal pouch-anal anastomosis, in which the colon is removed and part of the distal ileum is used to construct a pelvic reservoir. Competence of the ileal pouch to ferment carbohydrates is associated with the absence of pouchitis. However, the extent to which bacterial fermentation takes place and whether it is affected by diet are unclear. We investigated fermentation of two nondigestible carbohydrates, Fructooligosaccharides and resistant starch, in 15 healthy patients with an ileal pouch by using a placebo-controlled crossover design (with glucose as the placebo). Apparent fermentability of Fructooligosaccharides was 83%; that of resistant starch was 46%. Resistant starch increased fecal excretion of butyrate by 69% whereas Fructooligosaccharides reduced excretion of amino acid-derived isobutyrate by 94% and of isovalerate by 77%. Fructooligosaccharides also significantly increased fecal weight (651 compared with 541 g/d) and breath-hydrogen excretion (286 compared with 85 ppm x h). Bacterial fermentation of nondigestible carbohydrates in pouches takes place to an appreciable extent and in a substrate-specific manner. The relation between such fermentation and inflammation of the pouch (pouchitis) deserves study.
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Bacterialfermentation of Fructooligosaccharides and resistantstarchin patientswithan ilealpouch analanastomosis1
1997Co-Authors: Martine S Alles, M.b. Katan, J. M. J. I. Salemans, K.m.j. Van Laere, M.j. Rozendaal, Fokko M. NagengastAbstract:Patients with large bowel disease may undergo ileal pouchâ€"analanastomosis, in which the colon is removed and part of the distal ileum is used to construct a pelvic reservoir. Competence of the ileal pouch to ferment carbohydrates is asso ciated with the absence of pouchitis. However,the extent to which bacterial fermentation takes place and whether it is affected by diet are unclear. We investigated fermentation of two nondigestible carbohydrates, Fructooligosaccharides and resistant starch, in 15 healthy patients with an ileal pouch by using a placebo-controlled crossover design (with glucose as the placebo). Apparent ferment ability of Fructooligosaccharides was 83%; that of resistant starch was 46%. Resistant starch increased fecal excretion of butyrate by 69% whereas Fructooligosaccharides reduced excretion of amino acidâ€"derivedisobutyrate by 94% and of isovalerate by 77%. Fruc tooligosaccharides also significantly increased fecal weight (651 compared with 541 g/d) and breath-hydrogen excretion (286 corn pared with 85 ppm X h). Bacterial fermentation of nondigestible carbohydrates in pouches takes place to an appreciable extent and in a substrate-specific manner. The relation between such fermen tation and inflammation of the pouch (pouchitis) deserves study. Am J Clin Nutr l997;66:1286â€"92.
Edelberto Silva - One of the best experts on this subject based on the ideXlab platform.
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Semibatch and continuous Fructooligosaccharides production by Aspergillus sp. N74 in a mechanically agitated airlift reactor
Journal of Chemical Technology & Biotechnology, 2009Co-Authors: L. A. Caicedo, Edelberto Silva, Oscar F. SánchezAbstract:BACKGROUND: Fructooligosaccharides are important sweeteners produced by sucrose biotransformation. Although fructooligosccharides production has been reported widely, most studies have been carried out at laboratory level. This study evaluates semibatch and continuous Fructooligosaccharides production by Aspergillus sp. N74 at bench scale in a mechanically agitated airlift. RESULTS: Sucrose biotransformation to Fructooligosaccharides was carried out with biomass harvested after 24 or 48 h of culture. For 6.21 ± 0.33 or 9.66 ± 0.62 g biomass dry weight L−1, the highest FOS yields were obtained at batch operating 62.1 and 66.4% after 26 or 6 h of reaction, respectively. Reduction in Fructooligosaccharides yield was observed for both biomass concentrations at semibatch operating, while a comparable yield was obtained during continuous operating (62.1% for 6.21 ± 0.33 g L−1 and a dilution rate 0.016 s−1, and 62.8% for 9.66 ± 0.62 g L−1 and a dilution rate 0.032 s−1). Nevertheless, 1-kestose formation was favored with biomass harvested after 24 h under any operating mode. CONCLUSION: Biomass concentration, reaction time and operating mode have a notable effect on Fructooligosaccharides yield and composition. 1-kestose, the most valuable fructooligosaccharide, was obtained in greatest proportion at a biomass concentration 6.21 ± 0.33 g L−1. Under the different operating modes, Aspergillus sp. N74 mycelia and the reactor described are presented as a feasible alternative for scaling up Fructooligosaccharides production. Copyright © 2008 Society of Chemical Industry
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Sucrose Biotransformation to Fructooligosaccharides by Aspergillus sp. N74 Free Cells
Food and Bioprocess Technology, 2008Co-Authors: Oscar F. Sánchez, Edelberto Silva, Ana M. Rodríguez, L. A. CaicedoAbstract:Fructooligosaccharide production with the fructosyltransferase from free cells of the native strain Aspergillus sp. N74 at laboratory level was evaluated. The biomass of the native strain Aspergillus sp. N74 was produced in a sucrose fermentation medium and was employed in the enzymatic reaction in solutions of sucrose and phosphate buffer, where pH, temperature, and initial sucrose concentration effect were evaluated. Fructooligosaccharides and reaction subproducts were identified and quantified by high-performance liquid chromatography. The enzyme produced by the strain Aspergillus sp. N74 possessed hydrolytic and transfructosylating activities that changed with process conditions. The best transfructosylating condition was obtained at 80 min reaction time at pH 5.5, 60 °C, and initial sucrose concentrations higher than 550 g L−1, with fructooligosaccharide production of about 50% w/w (based on initial sucrose concentration) and conversion selectivity higher than 90%. In addition, the transfructosylating and hydrolytic activities ratio was of 20. The high transfructosylating activity showed by the fructosyltransferase produced from the native strain Aspergillus sp. N74 suggest considering it as an alternative for the scale-up production of Fructooligosaccharides by means of the whole microorganism at bench and pilot plant levels.
