The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Fang Wang - One of the best experts on this subject based on the ideXlab platform.
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production of fumaric acid by immobilized rhizopus arrhizus rh 7 13 9 on loofah fiber in a stirred tank reactor
Bioresource Technology, 2017Co-Authors: Shijie Zhao, Li Deng, Fang WangAbstract:Abstract Fumaric acid is an important building-block chemical. The production of fumaric acid by fermentation is possible. Loofah fiber is a natural, biodegradable, renewable polymer material with highly sophisticated and pore structure. This work investigated a new immobilization method using loofah fiber as carrier to produce fumaric acid in a stirred-tank reactor. Compared with other carriers, loofah fiber was proven to be efficiently and successfully used in the reactor. After the optimization process, 20 g addition of loofah fiber and 400 rpm agitation speed were chosen as the most suitable process conditions. 30.3 g/L fumaric acid in the broth as well as 19.16 g fumaric acid in the precipitation of solid was achieved, while the yield from glucose reached 0.211 g/g. Three batches of fermentation using the same loofah fiber carrier were conducted successfully, which meant it provided a new method to produce fumaric acid in a stirred-tank reactor.
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co fermentation of a mixture of glucose and xylose to fumaric acid by rhizopus arrhizus rh 7 13 9
Bioresource Technology, 2017Co-Authors: Huirong Hu, Li Deng, Fang WangAbstract:Abstract Lignocellulose is the most abundant biomass, composed of cellulose, hemicellulose and lignin. It can be converted into glucose and xylose, which could be utilized as carbon source to produce fumaric acid. But glucose and xylose were commonly used separately to produce fumaric acid, while the co-fermentation of glucose and xylose process was not studied so far. In this work, the co-fermentation process was researched through a new strain R. arrhizus RH 7 - 13-9# isolated from high concentration xylose. It was firstly proven to utilize glucose efficiently and 37.52 g/L fumaric acid was obtained from 80 g/L glucose. Furthermore, the effect of different ratios of glucose/xylose and carbon/nitrogen in the co-fermentation process was investigated and the best ratios were 75/25 (w/w) and 800/1 (w/w), where the yield of fumaric acid reached 46.78 g/L.
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food waste fermentation to fumaric acid by rhizopus arrhizus rh7 13
Applied Biochemistry and Biotechnology, 2016Co-Authors: Meng Wang, Weinan Wang, Li Deng, Fang WangAbstract:Fumaric acid as a four-carbon unsaturated dicarboxylic acid is widely used in the food and chemical industries. Food waste (FW), rich in carbohydrates and protein, is a promising potential feedstock for renewable bio-based chemicals. In this research, we investigated the capability of Rhizopus arrhizus RH7-13 in producing fumaric acid from FW. The liquid fraction of the FW (L-FW) was proven to be the best seed culture medium in our research. When it was however used to be fermentation medium, the yield of fumaric acid reached 32.68 g/L, at a volumetric production of 0.34 g/L h. The solid fraction of FW mixed with water (S-FW) could also be used as fermentation medium when a certain amount of glucose was added, and the yield of fumaric acid reached 31.26 g/L. The results indicated that both fractions of FW could be well utilized in fermentation process and it could replace a part of common carbon, nitrogen, and nutrient. The process has an application potential since reducing the costs of raw materials.
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high production of fumaric acid from xylose by newly selected strain rhizopus arrhizus rh 7 13 9
Bioresource Technology, 2015Co-Authors: Weinan Wang, Li Deng, Fang WangAbstract:Abstract Fumaric acid, as an important material for polymerization, is highly expected to be produced by fermentation of lignocellulosic biomass which is composed of cellulose, hemicellulose and lignin. Xylose as the main component of hemicellulose cannot be efficiently utilized by most of the common fermentation. In this study, a new strain Rhizopus arrhizus RH 7-13-9# was selected from the R. arrhizus RH 7-13 through a novel convenient and efficient selection method. Efficient production of fumaric acid (45.31 g/L) from xylose was achieved by the new strain, and the volumetric productivity was still 0.472 g/L h. Moreover, the conversion of xylose reached 73% which is close to the theoretic yield (77%). The production of fumaric acid was increased approximate by 172%, compared with the initial strain counterpart. These results indicated that xylose, as the main component of hemicellulose, has a promising application for the production of fumaric acid on an industrial-scale.
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production of fumaric acid from biodiesel derived crude glycerol by rhizopus arrhizus
Bioresource Technology, 2014Co-Authors: Yuqing Zhou, Li Deng, Meng Wang, Xin Zhang, Fang WangAbstract:Abstract This work investigated the capability of Rhizopus arrhizus to assimilate biodiesel-derived crude glycerol and convert it into fumaric acid. After optimizing the initial glycerol concentration, spore inoculum and yeast extract concentration, smaller pellets (0.7 mm) and higher biomass (3.11 g/L) were obtained when R. arrhizus grew on crude glycerol. It was found that crude glycerol was more suitable than glucose for smaller R. arrhizus pellet forming. When 80 g/L crude glycerol was used as carbon source, the fumaric acid production of 4.37 g/L was obtained at 192 h. With a highest concentration of 22.81 g/L achieved in the co-fermentation of crude glycerol (40 g/L) and glucose (40 g/L) at 144 h, the fumaric acid production was enhanced by 553.6%, compared to the fermentation using glycerol (80 g/L) as sole carbon source. Moreover, the production cost of fumaric acid in co-fermentation was reduced by approximately 14% compared to glucose fermentation.
Li Deng - One of the best experts on this subject based on the ideXlab platform.
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production of fumaric acid by immobilized rhizopus arrhizus rh 7 13 9 on loofah fiber in a stirred tank reactor
Bioresource Technology, 2017Co-Authors: Shijie Zhao, Li Deng, Fang WangAbstract:Abstract Fumaric acid is an important building-block chemical. The production of fumaric acid by fermentation is possible. Loofah fiber is a natural, biodegradable, renewable polymer material with highly sophisticated and pore structure. This work investigated a new immobilization method using loofah fiber as carrier to produce fumaric acid in a stirred-tank reactor. Compared with other carriers, loofah fiber was proven to be efficiently and successfully used in the reactor. After the optimization process, 20 g addition of loofah fiber and 400 rpm agitation speed were chosen as the most suitable process conditions. 30.3 g/L fumaric acid in the broth as well as 19.16 g fumaric acid in the precipitation of solid was achieved, while the yield from glucose reached 0.211 g/g. Three batches of fermentation using the same loofah fiber carrier were conducted successfully, which meant it provided a new method to produce fumaric acid in a stirred-tank reactor.
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co fermentation of a mixture of glucose and xylose to fumaric acid by rhizopus arrhizus rh 7 13 9
Bioresource Technology, 2017Co-Authors: Huirong Hu, Li Deng, Fang WangAbstract:Abstract Lignocellulose is the most abundant biomass, composed of cellulose, hemicellulose and lignin. It can be converted into glucose and xylose, which could be utilized as carbon source to produce fumaric acid. But glucose and xylose were commonly used separately to produce fumaric acid, while the co-fermentation of glucose and xylose process was not studied so far. In this work, the co-fermentation process was researched through a new strain R. arrhizus RH 7 - 13-9# isolated from high concentration xylose. It was firstly proven to utilize glucose efficiently and 37.52 g/L fumaric acid was obtained from 80 g/L glucose. Furthermore, the effect of different ratios of glucose/xylose and carbon/nitrogen in the co-fermentation process was investigated and the best ratios were 75/25 (w/w) and 800/1 (w/w), where the yield of fumaric acid reached 46.78 g/L.
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food waste fermentation to fumaric acid by rhizopus arrhizus rh7 13
Applied Biochemistry and Biotechnology, 2016Co-Authors: Meng Wang, Weinan Wang, Li Deng, Fang WangAbstract:Fumaric acid as a four-carbon unsaturated dicarboxylic acid is widely used in the food and chemical industries. Food waste (FW), rich in carbohydrates and protein, is a promising potential feedstock for renewable bio-based chemicals. In this research, we investigated the capability of Rhizopus arrhizus RH7-13 in producing fumaric acid from FW. The liquid fraction of the FW (L-FW) was proven to be the best seed culture medium in our research. When it was however used to be fermentation medium, the yield of fumaric acid reached 32.68 g/L, at a volumetric production of 0.34 g/L h. The solid fraction of FW mixed with water (S-FW) could also be used as fermentation medium when a certain amount of glucose was added, and the yield of fumaric acid reached 31.26 g/L. The results indicated that both fractions of FW could be well utilized in fermentation process and it could replace a part of common carbon, nitrogen, and nutrient. The process has an application potential since reducing the costs of raw materials.
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high production of fumaric acid from xylose by newly selected strain rhizopus arrhizus rh 7 13 9
Bioresource Technology, 2015Co-Authors: Weinan Wang, Li Deng, Fang WangAbstract:Abstract Fumaric acid, as an important material for polymerization, is highly expected to be produced by fermentation of lignocellulosic biomass which is composed of cellulose, hemicellulose and lignin. Xylose as the main component of hemicellulose cannot be efficiently utilized by most of the common fermentation. In this study, a new strain Rhizopus arrhizus RH 7-13-9# was selected from the R. arrhizus RH 7-13 through a novel convenient and efficient selection method. Efficient production of fumaric acid (45.31 g/L) from xylose was achieved by the new strain, and the volumetric productivity was still 0.472 g/L h. Moreover, the conversion of xylose reached 73% which is close to the theoretic yield (77%). The production of fumaric acid was increased approximate by 172%, compared with the initial strain counterpart. These results indicated that xylose, as the main component of hemicellulose, has a promising application for the production of fumaric acid on an industrial-scale.
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production of fumaric acid from biodiesel derived crude glycerol by rhizopus arrhizus
Bioresource Technology, 2014Co-Authors: Yuqing Zhou, Li Deng, Meng Wang, Xin Zhang, Fang WangAbstract:Abstract This work investigated the capability of Rhizopus arrhizus to assimilate biodiesel-derived crude glycerol and convert it into fumaric acid. After optimizing the initial glycerol concentration, spore inoculum and yeast extract concentration, smaller pellets (0.7 mm) and higher biomass (3.11 g/L) were obtained when R. arrhizus grew on crude glycerol. It was found that crude glycerol was more suitable than glucose for smaller R. arrhizus pellet forming. When 80 g/L crude glycerol was used as carbon source, the fumaric acid production of 4.37 g/L was obtained at 192 h. With a highest concentration of 22.81 g/L achieved in the co-fermentation of crude glycerol (40 g/L) and glucose (40 g/L) at 144 h, the fumaric acid production was enhanced by 553.6%, compared to the fermentation using glycerol (80 g/L) as sole carbon source. Moreover, the production cost of fumaric acid in co-fermentation was reduced by approximately 14% compared to glucose fermentation.
Kyung-bin Song - One of the best experts on this subject based on the ideXlab platform.
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combined treatment with chlorine dioxide gas fumaric acid and ultraviolet c light for inactivating escherichia coli o157 h7 and listeria monocytogenes inoculated on plums
Food Control, 2017Co-Authors: Kyung-bin SongAbstract:Abstract Combined non-thermal treatment with chlorine dioxide (ClO2) gas, ultraviolet-C (UV-C) light, and fumaric acid was performed to inactivate Listeria monocytogenes and Escherichia coli O157:H7 inoculated on plums. Plums were treated with ClO2 gas (15 and 30 ppmv), fumaric acid (0.1, 0.3, and 0.5%), and by UV-C irradiation (3, 5, and 10 kJ/m2). The single treatments with 15 or 30 ppmv ClO2 gas, 0.5% fumaric acid, and 10 kJ/m2 UV-C decreased the population of L. monocytogenes by 1.78, 2.00, 1.65, and 1.62 log CFU/g, respectively, and the population of E. coli O157:H7 by 1.73, 1.81, 1.34, and 2.07 log CFU/g, respectively. In addition, combined treatments reduced the populations of the pathogenic bacteria more than each treatment alone. In particular, the combined treatment with ClO2 gas (30 ppmv) for 20 min, fumaric acid (0.5%), and UV-C (10 kJ/m2) decreased the populations of L. monocytogenes and E. coli O157:H7 by 6.26 and 5.48 log CFU/g, respectively. These results suggest that combined treatment with ClO2 gas, UV-C light, and fumaric acid may be a useful hurdle technology to enhance the microbiological safety of plums.
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combined treatment of aqueous chlorine dioxide organic acid and blanching for microbial decontamination of wild vegetables after harvest
Journal of The Korean Society of Food Science and Nutrition, 2016Co-Authors: Jihoon Kang, Shin Min Park, Kilnam Kang, Jongtae Park, Kyung-bin SongAbstract:To improve the microbiological safety of wild vegetables after harvest, Aster scaber and Cirsium setidens Nakai were treated with combinations of 50 ppm aqueous chlorine dioxide (ClO2)/0.5% citric acid or fumaric acid, and 50 ppm ClO2/0.5% fumaric acid/blanching at 90°C for 2 min. Combined treatment of 50 ppm ClO2 and 0.5% citric acid reduced populations of total aerobic bacteria, yeast, and molds in Aster scaber and Cirsium setidens Nakai by 2.80∼3.64 and 2.02∼2.67 log CFU/g, respectively, compared to those of the control. Combined treatment of 50 ppm ClO2 and 0.5% fumaric acid reduced total aerobic bacteria, yeast and molds populations by 3.62∼3.82 and 2.47∼3.02 log CFU/g, respectively. Based on the results, combined treatment of ClO2 and fumaric acid was more effective in controlling microorganisms in the wild vegetables than either ClO2 or citric acid. In addition, combined treatment of ClO2/fumaric acid/blanching reduced the populations of total aerobic bacteria by 4.59∼5.12 log CFU/g, and populations of yeast and molds were not detected by treatment. These results suggest that combined treatment of ClO2/fumaric acid/blanching is the most effective method for improving microbiological safety of wild vegetables after harvest.
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the effects of aqueous chlorine dioxide or fumaric acid treatment combined with uv c on postharvest quality of maehyang strawberries
Postharvest Biology and Technology, 2010Co-Authors: Sung A Jang, Kyung-bin SongAbstract:Abstract The combined effect of aqueous chlorine dioxide (ClO 2 ) or fumaric acid with ultraviolet-C (UV-C) on postharvest quality of “Maehyang” strawberries was examined. The strawberries were treated with distilled water, 50 mg L −1 ClO 2 , 0.5% fumaric acid, 5 kJ m −2 UV-C irradiation, and a combination of 50 mg L −1 ClO 2 /5 kJ m −2 UV-C and 0.5% fumaric acid/5 kJ m −2 UV-C. The combined treatment of fumaric acid/UV-C reduced the initial populations of total aerobic bacteria and yeast and molds in the strawberries by 2.25 and 2.01 log CFU g −1 , respectively. Sensory evaluation results indicated that the combined treatment provided better sensory scores than did the control. These results suggest that postharvest treatments of either ClO 2 or fumaric acid with UV-C can be useful for maintaining the quality of ‘Maehyang’ strawberries.
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combined treatment of fumaric acid with aqueous chlorine dioxide or uv c irradiation to inactivate escherichia coli o157 h7 salmonella enterica serovar typhimurium and listeria monocytogenes inoculated on alfalfa and clover sprouts
Lwt - Food Science and Technology, 2009Co-Authors: Kyung-bin SongAbstract:Abstract Effect of fumaric acid, chlorine dioxide (ClO2), and UV-C treatment was examined on the inactivation of microorganisms in alfalfa and clover sprouts. Clover sprouts were irradiated with UV-C light (1–10 kJ/m2), and the treatment decreased the population of total aerobic bacteria by 1.03–1.45 log CFU/g. Clover sprouts inoculated with pathogenic bacteria were treated with various concentration of fumaric acid, and 0.5 g/100 ml fumaric acid treatment was the most effective. In addition, the combined treatment of fumaric acid (0.5 g/100 ml)/UV-C (1 kJ/m2) reduced the populations of Escherichia coli O157:H7, Salmonella enterica serovar Typhimurium, and Listeria monocytogenes inoculated on clover sprouts by 3.02, 2.88, and 2.35 log CFU/g. Alfalfa sprouts were treated with ClO2, fumaric acid, and the combination of fumaric acid/ClO2. The combined treatment was the most effective, and it reduced the total aerobic bacteria by 3.18 log CFU/g as well as the initial populations of E. coli O157:H7, S. typhimurium, and L. monocytogenes inoculated on alfalfa sprouts by 4.06, 3.57, and 3.69 log CFU/g. These results suggest that the combined treatment of fumaric acid with UV-C or ClO2 can be useful for improving the microbial safety of alfalfa and clover sprouts.
Krystyna Prochaska - One of the best experts on this subject based on the ideXlab platform.
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fumaric acid separation from fermentation broth using nanofiltration nf and bipolar electrodialysis edbm
Separation and Purification Technology, 2014Co-Authors: Marta Woźniak, Krystyna ProchaskaAbstract:Abstract The suitability of two techniques: nanofiltration (NF) with ceramic membrane and bipolar electrodialysis (EDBM), in separation and concentration of fumaric acid from fermentation broth obtained during biotechnological conversion of glycerol, was investigated. The influence of composition, concentration and pH of fumaric acid model solutions on the efficiency of nanofiltration process was studied. It was found that the retention of fumaric salts increased strongly with increasing pH of the initial solution, while the retention of glycerol was lower than 6%, irrespective of pH. In the second step, the influence of electrodialysis stack configuration, current density, pH and concentration of fumaric acid in model solutions on the efficiency of bipolar electrodialysis was analysed. Then, after NF pretreatment, EDBM process of simulated solutions as well as fermentation broth was performed. The current density equal to 50 A/m2 was found sufficient to achieve a 53% desalination of fumaric acid from fermentation broth. Moreover, the results obtained showed that bipolar electrodialysis stack, consisting of anion-exchange and bipolar membranes, allowed selective separation of fumaric acid from the broth. The results suggest that NF with ceramic membrane as well as EDBM can be effectively applied as a removal step of fumaric acid from fermentation broth.
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fumaric acid separation from fermentation broth using nanofiltration nf and bipolar electrodialysis edbm
Separation and Purification Technology, 2014Co-Authors: Marta Woźniak, Krystyna ProchaskaAbstract:Abstract The suitability of two techniques: nanofiltration (NF) with ceramic membrane and bipolar electrodialysis (EDBM), in separation and concentration of fumaric acid from fermentation broth obtained during biotechnological conversion of glycerol, was investigated. The influence of composition, concentration and pH of fumaric acid model solutions on the efficiency of nanofiltration process was studied. It was found that the retention of fumaric salts increased strongly with increasing pH of the initial solution, while the retention of glycerol was lower than 6%, irrespective of pH. In the second step, the influence of electrodialysis stack configuration, current density, pH and concentration of fumaric acid in model solutions on the efficiency of bipolar electrodialysis was analysed. Then, after NF pretreatment, EDBM process of simulated solutions as well as fermentation broth was performed. The current density equal to 50 A/m2 was found sufficient to achieve a 53% desalination of fumaric acid from fermentation broth. Moreover, the results obtained showed that bipolar electrodialysis stack, consisting of anion-exchange and bipolar membranes, allowed selective separation of fumaric acid from the broth. The results suggest that NF with ceramic membrane as well as EDBM can be effectively applied as a removal step of fumaric acid from fermentation broth.
Weinan Wang - One of the best experts on this subject based on the ideXlab platform.
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food waste fermentation to fumaric acid by rhizopus arrhizus rh7 13
Applied Biochemistry and Biotechnology, 2016Co-Authors: Meng Wang, Weinan Wang, Li Deng, Fang WangAbstract:Fumaric acid as a four-carbon unsaturated dicarboxylic acid is widely used in the food and chemical industries. Food waste (FW), rich in carbohydrates and protein, is a promising potential feedstock for renewable bio-based chemicals. In this research, we investigated the capability of Rhizopus arrhizus RH7-13 in producing fumaric acid from FW. The liquid fraction of the FW (L-FW) was proven to be the best seed culture medium in our research. When it was however used to be fermentation medium, the yield of fumaric acid reached 32.68 g/L, at a volumetric production of 0.34 g/L h. The solid fraction of FW mixed with water (S-FW) could also be used as fermentation medium when a certain amount of glucose was added, and the yield of fumaric acid reached 31.26 g/L. The results indicated that both fractions of FW could be well utilized in fermentation process and it could replace a part of common carbon, nitrogen, and nutrient. The process has an application potential since reducing the costs of raw materials.
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high production of fumaric acid from xylose by newly selected strain rhizopus arrhizus rh 7 13 9
Bioresource Technology, 2015Co-Authors: Weinan Wang, Li Deng, Fang WangAbstract:Abstract Fumaric acid, as an important material for polymerization, is highly expected to be produced by fermentation of lignocellulosic biomass which is composed of cellulose, hemicellulose and lignin. Xylose as the main component of hemicellulose cannot be efficiently utilized by most of the common fermentation. In this study, a new strain Rhizopus arrhizus RH 7-13-9# was selected from the R. arrhizus RH 7-13 through a novel convenient and efficient selection method. Efficient production of fumaric acid (45.31 g/L) from xylose was achieved by the new strain, and the volumetric productivity was still 0.472 g/L h. Moreover, the conversion of xylose reached 73% which is close to the theoretic yield (77%). The production of fumaric acid was increased approximate by 172%, compared with the initial strain counterpart. These results indicated that xylose, as the main component of hemicellulose, has a promising application for the production of fumaric acid on an industrial-scale.
