The Experts below are selected from a list of 252 Experts worldwide ranked by ideXlab platform
Nikhil S. Shaligram - One of the best experts on this subject based on the ideXlab platform.
-
biosynthesis of silver nanoparticles using aqueous extract from the compactin producing fungal strain
Process Biochemistry, 2009Co-Authors: Nikhil S. Shaligram, Rekha S. Singhal, Mahesh V Bule, Rahul Bhambure, Sudheer Kumar Singh, George Szakacs, Ashok PandeyAbstract:In the present study, an eco-friendly process for the synthesis of nanomaterials using a fungus, Penicillium brevicompactum WA 2315 has been attempted. The fungus has been previously utilized for compactin production. Supernatant of Seed Culture was used for the biosynthesis of silver nanoparticles. The aqueous silver ions were reduced to silver metal nanoparticles when treated with the fungal supernatant. After 72 h of treatment, silver nanoparticles obtained were in the range of 23–105 nm as obtained from TEM. The nanoparticles were characterized by UV, FTIR, SEM, TEM and XRD. The use of supernatant of the Seed media of the said fungus opens up the exciting possibility of rational strategy of biosynthesis of nanomaterials.
-
A novel medium for the enhanced production of cyclosporin A by Tolypocladium inflatum MTCC 557 using solid state fermentation.
Journal of microbiology and biotechnology, 2009Co-Authors: Shrikant A. Survase, Nikhil S. Shaligram, Ruchir C. Pansuriya, Uday S. AnnapureAbstract:Cyclosporin A (CyA) produced by Tolypocladium inflatum is a promising drug owing to its immunosuppressive and antifungal activities. From an industrial point of view, the necessity to obtain a suitable and economic medium for higher production of CyA was the aim of this work. The present study evaluated the effect of different fermentation parameters in solid state fermentation, such as selection of solid substrate, hydrolysis of substrates, initial moisture content, supplementation of salts, additional carbon, and nitrogen sources, as well as the inoculum age and size, on production of CyA by Tolypocladium inflatum MTCC 557. The fermentation was carried out at 25+/-2 degrees for 9 days. A combination of hydrolyzed wheat bran flour and coconut oil cake (1:1) at 70% initial moisture content supported a maximum production of 3,872+/-156 mg CyA/kg substrate as compared with 792+/-33 mg/kg substrate before optimization. Furthermore, supplementation of salts, glycerol (1%w/w), and ammonium sulfate (1%w/w) increased the production of CyA to 5,454+75 mg/kg substrate. Inoculation of 5 g of solid substrate with 6 ml of 72-h-old Seed Culture resulted in a maximum production of 6,480+95 mg CyA/kg substrate.
-
A Novel Medium for the Enhanced Production of Cyclosporin A by Tolypocladium inflatum MTCC 557 Using Solid State Fermentation
2008Co-Authors: Shrikant A, Uday S. Annapure, Nikhil S. Shaligram, Ruchir C. Pansuriya, Rekha S. SinghalAbstract:a promising drug owing to its immunosuppressive and antifungal activities. From an industrial point of view, the necessity to obtain a suitable and economic medium for higher production of CyA was the aim of this work. The present study evaluated the effect of different fermentation parameters in solid state fermentation, such as selection of solid substrate, hydrolysis of substrates, initial moisture content, supplementation of salts, additional carbon, and nitrogen sources, as well as the inoculum age and size, on production of CyA by Tolypocladium inflatum MTCC 557. The fermentation was carried out at 25±2 o C for 9 days. A combination of hydrolyzed wheat bran flour and coconut oil cake (1:1) at 70 % initial moisture content supported a maximum production of 3,872±156 mg CyA/kg substrate as compared with 792±33 mg/kg substrate before optimization. Furthermore, supplementation of salts, glycerol (1 % w/w), and ammonium sulfate (1 % w/w) increased the production of CyA to 5,454±75 mg/kg substrate. Inoculation of 5 g of solid substrate with 6 ml of 72-h-old Seed Culture resulted in a maximum production of 6,480±95 mg CyA/kg substrate
Rekha S. Singhal - One of the best experts on this subject based on the ideXlab platform.
-
biosynthesis of silver nanoparticles using aqueous extract from the compactin producing fungal strain
Process Biochemistry, 2009Co-Authors: Nikhil S. Shaligram, Rekha S. Singhal, Mahesh V Bule, Rahul Bhambure, Sudheer Kumar Singh, George Szakacs, Ashok PandeyAbstract:In the present study, an eco-friendly process for the synthesis of nanomaterials using a fungus, Penicillium brevicompactum WA 2315 has been attempted. The fungus has been previously utilized for compactin production. Supernatant of Seed Culture was used for the biosynthesis of silver nanoparticles. The aqueous silver ions were reduced to silver metal nanoparticles when treated with the fungal supernatant. After 72 h of treatment, silver nanoparticles obtained were in the range of 23–105 nm as obtained from TEM. The nanoparticles were characterized by UV, FTIR, SEM, TEM and XRD. The use of supernatant of the Seed media of the said fungus opens up the exciting possibility of rational strategy of biosynthesis of nanomaterials.
-
A Novel Medium for the Enhanced Production of Cyclosporin A by Tolypocladium inflatum MTCC 557 Using Solid State Fermentation
2008Co-Authors: Shrikant A, Uday S. Annapure, Nikhil S. Shaligram, Ruchir C. Pansuriya, Rekha S. SinghalAbstract:a promising drug owing to its immunosuppressive and antifungal activities. From an industrial point of view, the necessity to obtain a suitable and economic medium for higher production of CyA was the aim of this work. The present study evaluated the effect of different fermentation parameters in solid state fermentation, such as selection of solid substrate, hydrolysis of substrates, initial moisture content, supplementation of salts, additional carbon, and nitrogen sources, as well as the inoculum age and size, on production of CyA by Tolypocladium inflatum MTCC 557. The fermentation was carried out at 25±2 o C for 9 days. A combination of hydrolyzed wheat bran flour and coconut oil cake (1:1) at 70 % initial moisture content supported a maximum production of 3,872±156 mg CyA/kg substrate as compared with 792±33 mg/kg substrate before optimization. Furthermore, supplementation of salts, glycerol (1 % w/w), and ammonium sulfate (1 % w/w) increased the production of CyA to 5,454±75 mg/kg substrate. Inoculation of 5 g of solid substrate with 6 ml of 72-h-old Seed Culture resulted in a maximum production of 6,480±95 mg CyA/kg substrate
Michael E. Kane - One of the best experts on this subject based on the ideXlab platform.
-
Asymbiotic and symbiotic Seed germination of Eulophia alta (Orchidaceae)—preliminary evidence for the symbiotic Culture advantage
Plant Cell Tissue and Organ Culture, 2007Co-Authors: Timothy R. Johnson, Michael E. Kane, Scott L. Stewart, Daniela Dutra, Larry RichardsonAbstract:Eulophia alta (Linnaeus) Fawcett & Rendle Seeds collected from the Florida Panther National Wildlife Refuge (Collier County, FL; FPNWR) were used in a screen of five asymbiotic orchid Seed germination media to determine their effectiveness in promoting germination and protocorm development. In addition, 10 fungal isolates collected from the roots of E. alta at sites in the FPNWR, Highlands County (FL), and Goethe State Forest (Levy County, FL; GSF), and a fungal isolate from the roots of Spiranthes brevilabris collected from GSF were screened for their effectiveness at promoting in vitro symbiotic germination of E. alta Seeds. After 18 weeks asymbiotic Culture, Seeds sown on Phyto Technology Orchid Seed Sowing Medium germinated to a higher percentage (87.9%) and had a higher percentage of protocorms with developing protomeristems (32.7%) than Seeds Cultured on Knudson C, Malmgren Modified Terrestrial Orchid Medium, ½-strength Murashige & Skoog, or Vacin & Went Modified Orchid Medium. Significantly more leaf-bearing protocorms were observed on Phyto Technology Orchid Seed Sowing Medium (0.8%) and Vacin & Went Modified Orchid Medium (1.3%) than other media tested. Of the fungi tested, one fungal isolate (Ealt-396) promoted germination to 69.0%, two isolates promoted germination to less than 0.75% and did not support further protocorm development, and eight isolates did not support germination. Seeds co-Cultured in darkness with Ealt-396 grew more rapidly than asymbiotic Seedlings following germination. In addition, co-Cultured (=symbiotic) Seedlings continued to develop more rapidly than asymbiotic Seedlings upon transfer to 16/8 h light/dark photoperiod. Symbiotic Seed Culture of E. alta may be a more desirable method of propagation since protocorms develop more rapidly than Seeds sown on asymbiotic media. Symbiotic Seedlings may be more appropriate for reintroduction to natural areas than asymbiotic Seedlings since symbiotic Seedlings could serve to inoculate soils with a germination promoting mycobiont.
-
In vitro Seed Culture and Seedling development of Calopogon tuberosus
Plant Cell Tissue and Organ Culture, 2006Co-Authors: Philip J. Kauth, Wagner A. Vendrame, Michael E. KaneAbstract:A major obstacle to native orchid production is difficulty in Seed germination. Culture media and light effects on Seed germination of Calopogon tuberosus var. tuberosus , a native orchid with horticultural potential, were studied. Culture media included Knudson C, Malmgren modified terrestrial orchid, and PhytoTechnology orchid Seed sowing. Effects of 8 weeks continual darkness, 8 weeks 16-h photoperiod, 2 weeks dark followed by 6 weeks 16-h photoperiod, 4 weeks dark followed by 4 weeks 16-h photoperiod, and 6 weeks dark followed by 2 weeks 16-h photoperiod were examined. Percent Seed germination was highest on Knudson C after 8 weeks Culture; however, Seedling development was enhanced on PhytoTechnology Seed sowing medium during 8 weeks Culture under a 16-h photoperiod. This suggests that while KC and darkness promoted Seed germination, P723 and light enhanced further Seedling development. Seedlings of C. tuberosus readily acclimated to greenhouse conditions.
V S Bisaria - One of the best experts on this subject based on the ideXlab platform.
-
effect of Seed Culture on solid state bioconversion of wheat straw by phanerochaete chrysosporium for animal feed production
Journal of Bioscience and Bioengineering, 2002Co-Authors: Shubhayu Basu, Rajneesh Kumar Gaur, James Gomes, T R Sreekrishnan, V S BisariaAbstract:The solid-state bioconversion of wheat straw by Phanerochaete chrysosporium for the production of animal feed was studied. This study was performed based on a central composite experimental design. The conditions of the Seed Culture most suitable for rapid induction of the ligninolytic activity of the fungus, when the Seed Culture is subsequently used for solid-state bioconversion of wheat straw, were determined. When the Seed Culture with an initial pH of 5.8 was grown under agitated conditions at 130 rpm in baffled flasks at 38°C, it was predicted to give lignin degradation of 19.5% and cellulose degradation of 17.8%. A time profile study of the solid-state bioconversion of wheat straw indicated that the highest lignin and lowest cellulose degradation levels occurred on the sixth day of cultivation. The desirability coefficient for this process also passed through a maximum of 0.705 on the sixth day.
Shubhayu Basu - One of the best experts on this subject based on the ideXlab platform.
-
effect of Seed Culture on solid state bioconversion of wheat straw by phanerochaete chrysosporium for animal feed production
Journal of Bioscience and Bioengineering, 2002Co-Authors: Shubhayu Basu, Rajneesh Kumar Gaur, James Gomes, T R Sreekrishnan, V S BisariaAbstract:The solid-state bioconversion of wheat straw by Phanerochaete chrysosporium for the production of animal feed was studied. This study was performed based on a central composite experimental design. The conditions of the Seed Culture most suitable for rapid induction of the ligninolytic activity of the fungus, when the Seed Culture is subsequently used for solid-state bioconversion of wheat straw, were determined. When the Seed Culture with an initial pH of 5.8 was grown under agitated conditions at 130 rpm in baffled flasks at 38°C, it was predicted to give lignin degradation of 19.5% and cellulose degradation of 17.8%. A time profile study of the solid-state bioconversion of wheat straw indicated that the highest lignin and lowest cellulose degradation levels occurred on the sixth day of cultivation. The desirability coefficient for this process also passed through a maximum of 0.705 on the sixth day.
