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Ferreira, Alana Rodrigues - One of the best experts on this subject based on the ideXlab platform.
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Avaliação da atividade antifúngica de ésteres benzoicos estruturalmente relacionados frente a espécies de Candida
Universidade Federal da Paraíba, 2016Co-Authors: Ferreira, Alana RodriguesAbstract:The benzoic derivatives have been related to antioxidant activity, anti-inflammatory, cytotoxic (cancer cell), antiviral, antibacterial and antiFungal, an growing interest in the scientific field. In the present study, it was investigated the antiFungal activity of a collection of benzoic acids esters derivatives, against the pathogenic species of Candida genus; causing the broad spectrum of Fungal infections known as candidiasis. It was us ed the esterification catalyzed by acid to obtain the evaluated esters and the antiFungal tests were performed with the microdilution broth method. The main objective of this study was to evaluate the influence of the presence and position of the various substituents of the aromatic ring of the esters, including: -OH, -CH3, -Ar, NO2, in the biological activity. The results were satisfactory. The bioactive substances showed good to moderate the bioactivity; the best MIC vary between 256μg / ml and 64μg / ml, this last one was presented by p-hydroxybenzoate methylester, against C. krusei and C.parapsilosis species. Some comments were made about the influence of substituents in this bioactivity: the methyl group in para position of the ring contributed to the inactivity of the compounds in question; the hydroxyl group in the same position improved bioactivity in some cases, being important for interaction with components of the Fungal Membrane through hydrog en bonding; the nitro group, through its reducing ability, may have been relevant to the bioactivity of the respective ester and the presence of a second aromatic ring in the ortho or para position, guarantee the bioactivity of the biphenyl substances. The results presented in the work presented relevance to the search of a compound with improved biological profile which can be used in the development of new antiFungal drugs.Os derivados benzoicos já foram relacionados à capacidade antioxidante, anti-inflamatória, citotóxica (de células cancerígenas), antiviral, antibacteriana e antifúngica, representando um interesse crescente no campo científico. No presente estudo, foi investigada a atividade antifúngica de uma coleção de ésteres derivados de ácidos benzoicos frente à espécies patogênicas do gênero Candida, causadores do amplo conjunto de infecções fúngicas conhecido por Candidíase. Utilizou-se a reação de esterificação catalisada por ácido para a obtenção dos ésteres avaliados e os testes antifúngicos foram realizados com o método de microdiluição em caldo. O principal objetivo do trabalho foi avaliar a influência da presença e posição dos diferentes grupos substituintes do anel aromático dos ésteres, entre eles: -OH, -CH3, -Ar, e -NO2, na atividade biológica. Os resultados mostraram-se satisfatórios, visto que as substâncias bioativas apresentaram de ótima à moderada bioatividade; as melhores CIM variaram entre 256μg/mL e 64μg/mL, esta última foi apresentada pelo p-hidroxibenzoato de metila, frente as espécies C. krusei e C.parapsilosis. Algumas observações foram feitas em relação a influência dos substituintes nessa bioatividade: a metila na posição parado anel contribuiu para a inatividade dos compostos em questão; a hidroxila na mesma posição Melhorou a bioatividade em alguns casos, sendo também importante para a interação com componentes das membranas fúngicas através de pontes de hidrogênio; o grupo nitro, através de sua capacidade redutora, pode ter sido relevante para a bioatividade do respectivo éster e a presença de um segundo anel aromático, nas posições ortoou para, garantiu a bioatividade das substâncias bifenílicas. Os resultados apresentados no estudo tem relevância na pesquisa de uma substância com melhor perfil biológico que possa então ser utilizado no desenvolvimento de novos fármacos antifúngicos
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Avaliação da atividade antifúngica de ésteres benzoicos estruturalmente relacionados frente a espécies de Candida
'Portal de Periodicos UFPB', 2016Co-Authors: Ferreira, Alana RodriguesAbstract:The benzoic derivatives have been related to antioxidant activity, anti-inflammatory, cytotoxic (cancer cell), antiviral, antibacterial and antiFungal, an growing interest in the scientific field. In the present study, it was investigated the antiFungal activity of a collection of benzoic acids esters derivatives, against the pathogenic species of Candida genus; causing the broad espectrum of Fungal infections known as candidiasis. It was used the esterification catalyzed by acid to obtain the evaluated esters and the antiFungal tests were performed with the microdilution broth method. The main objective of this study was to evaluate the influence of the presence and position of the various substituents of the aromatic ring of the esters, including: -OH, -CH3, -Ar, NO2, in the biological activity. The results were satisfactory. The bioactive substances showed good to moderate the bioactivity; the best MIC vary between 256μg / ml and 64μg / ml, this last one was presented by p-hydroxybenzoate methyl ester, against C. krusei and C. parapsilosis species. Some comments were made about the influence of substituents in this bioactivity: the methyl group in para position of the ring contributed to the inactivity of the compounds in question; the hydroxyl group in the same position improved bioactivity in some cases, being important for interaction with components of the Fungal Membrane through hydrogen bonding; the nitro group, through its reducing ability, may have been relevant to the bioactivity of the respective ester and the presence of a second aromatic ring in the ortho or para position, guarantee the bioactivity of the biphenyl substances. The results presented in the work presented relevance to the search of a compound with improved biological profile which can be used in the development of new antiFungal drugs.Os derivados benzoicos já foram relacionados à capacidade antioxidante, anti-inflamatória, citotóxica (de células cancerígenas), antiviral, antibacteriana e antifúngica, representando um interesse crescente no campo científico. No presente estudo, foi investigada a atividade antifúngica de uma coleção de ésteres derivados de ácidos benzoicos frente à espécies patogênicas do gênero Candida, causadores do amplo conjunto de infecções fúngicas conhecido por Candidíase. Utilizou-se a reação de esterificação catalisada por ácido para a obtenção dos ésteres avaliados e os testes antifúngicos foram realizados com o método de microdiluição em caldo. O principal objetivo do trabalho foi avaliar a influência da presença e posição dos diferentes grupos substituintes do anel aromático dos ésteres, entre eles: -OH, -CH3, -Ar, e -NO2, na atividade biológica. Os resultados mostraram-se satisfatórios, visto que as substâncias bioativas apresentaram de ótima à moderada bioatividade; as melhores CIM variaram entre 256μg/mL e 64μg/mL, esta última foi apresentada pelo p-hidroxibenzoato de metila, frente as espécies C. krusei e C. parapsilosis. Algumas observações foram feitas em relação a influência dos substituintes nessa bioatividade: a metila na posição para do anel contribuiu para a inatividade dos compostos em questão; a hidroxila na mesma posição melhorou a bioatividade em alguns casos, sendo também importante para a interação com componentes das membranas fúngicas através de pontes de hidrogênio; o grupo nitro, através de sua capacidade redutora, pode ter sido relevante para a bioatividade do respectivo éster e a presença de um segundo anel aromático, nas posições orto ou para, garantiu a bioatividade das substâncias bifenílicas. Os resultados apresentados no estudo tem relevância na pesquisa de uma substância com melhor perfil biológico que possa então ser utilizado no desenvolvimento de novos fármacos antifúngicos
Ece Ummu Deveci - One of the best experts on this subject based on the ideXlab platform.
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degradation of recalcitrant textile dyes by coupling Fungal and photocatalytic Membrane reactors
Clean-soil Air Water, 2016Co-Authors: Ece Ummu Deveci, Nadir Dizge, Huseyin Cengiz Yatmaz, Berrin TanselAbstract:White rot fungi have powerful and broad specific extracellular enzymatic systems and can degrade multiple types of recalcitrant compounds including azo dyes, therefore, they are used in the field of bioremediation. In the present study, decolorization and organic matter removal of Acid Red 88 (AR88) and Reactive Red 180 (RR180) dye solutions were performed using the white rot fungus Phanerochaete chrysosporium in a bioreactor. Degradation of the dyes was assessed using a hybrid process consisting of a Fungal Membrane bioreactor coupled with a photocatalytic Membrane reactor. The photocatalytic oxidation step was conducted using the semiconductor ZnO as the catalyst with UVA irradiation. Experimental data with the Fungal Membrane bioreactor showed that the optimum pH was 4.5 for P. chrysosporium. Integrating photocatalytic step after the Fungal Membrane process improved the chemical oxygen demand (COD) removal efficiency up to 98 and 96% for RR180 and AR88 dye solutions, respectively. Color removal efficiencies after the photocatalytic degradation were 99.9% for both dye solutions.
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integrated process of Fungal Membrane bioreactor and photocatalytic Membrane reactor for the treatment of industrial textile wastewater
Biochemical Engineering Journal, 2016Co-Authors: Ece Ummu Deveci, Nadir Dizge, Cengiz H Yatmaz, Yasin AytepeAbstract:Abstract In this study, Fungal Membrane bioreactor (FMBR) and semiconductor photocatalytic Membrane reactor (PMR) were used in order to test the efficiency of integrated Fungal biodegradation and photocatalytic degradation of textile wastewater from reactive washing processes. It was found that color removal and chemical oxygen demand (COD) reduction efficiencies were 88% and 53% for photocatalytic degradation, respectively. TiO2 and ZnO were tested as semiconductor catalysts in the PMR and TiO2 showed better efficiencies than ZnO for both color and COD removal. However, it was attained that color removal and COD reduction efficiencies were about 56% and 60% for Fungal biodegradation using Phanerochaete chrysosporium, respectively. Moreover, integrated system in which photocatalytic degradation was employed as a post-treatment application after Fungal biodegradation process achieved high removal efficiencies for color and COD removal as 93% and 99%, respectively.
Karin Thevissen - One of the best experts on this subject based on the ideXlab platform.
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neutral glycolipids of the filamentous fungus neurospora crassa altered expression in plant defensin resistant mutants
Journal of Lipid Research, 2005Co-Authors: Chaeho Park, Bruno P. A. Cammue, Karin Thevissen, Beau Bennion, Isabelle E J A Francois, Kathelijne K A Ferket, Steven B LeveryAbstract:To defend themselves against Fungal pathogens, plants produce numerous antiFungal proteins and peptides, including defensins, some of which have been proposed to interact with Fungal cell surface glycosphingolipid components. Although not known as a phytopathogen, the filamentous fungus Neurospora crassa possesses numerous genes similar to those required for plant pathogenesis identified in Fungal pathogens (Galagan, J. E., et al. 2003. Nature 422: 859-868), and it has been used as a model for studying plant-phytopathogen interactions targeting Fungal Membrane components (Thevissen, K., et al. 2003. Peptides. 24: 1705-1712). For this study, neutral glycolipid components were extracted from wild-type and plant defensin-resistant mutant strains of N. crassa. The structures of purified components were elucidated by NMR spectroscopy and mass spectrometry. Neutral glycosphingolipids of both wild-type and mutant strains were characterized as beta-glucopyranosylceramides, but those of the mutants were found with structurally altered ceramides. Although the wild type expressed a preponderance of N-2'-hydroxy-(E)-Delta3-octadecenoate as the fatty-N-acyl component attached to the long-chain base (4E,8E)-9-methyl-4,8-sphingadienine, the mutant ceramides were found with mainly N-2'-hydroxyhexadecanoate instead. In addition, the mutant strains expressed highly increased levels of a sterol glucoside identified as ergosterol-beta-glucoside. The potential implications of these findings with respect to defensin resistance in the N. crassa mutants are discussed.
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Interactions of antiFungal plant defensins with Fungal Membrane components.
Peptides, 2003Co-Authors: Karin Thevissen, Isabelle E J A Francois, Kathelijne K A Ferket, Bruno P. A. CammueAbstract:Plant defensins are small, basic, cysteine-rich peptides that are generally active against a broad spectrum of Fungal and yeast species at micromolar concentrations. Some of these defensins interact with Fungal-specific lipid components in the plasmaMembrane. Structural differences of these Membrane components between Fungal and plant cells probably account for the selective activity of plant defensins against Fungal pathogens and their nonphytotoxic properties. This review will focus on different classes of complex lipids in Fungal Membranes and on the selective interaction of plant defensins with these complex lipids.
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Fungal Membrane Responses Induced by Plant Defensins and Thionins
Journal of Biological Chemistry, 1996Co-Authors: Karin Thevissen, Rupert W. Osborn, Genoveva W. De Samblanx, Alexandre Ghazi, Willem F. BroekaertAbstract:Abstract Treatment of hyphae of Neurospora crassa with antiFungal plant defensins, i.e. Rs-AFP2 and Dm-AMP1 isolated from radish and dahlia seed, respectively, induced a rapid K+ efflux, Ca2+ uptake, and alkalinization of the incubation medium. The Rs-AFP2-induced alkalinization of the incubation medium could be inhibited with G-protein inhibitors. α-Hordothionin, an antiFungal thionin from barley seed, caused a sustained increased Ca2+ uptake at subinhibitory concentrations but only a transient increased uptake at inhibitory concentrations. α-Hordothionin also caused increased K+ efflux and alkalinization of the medium, but these fluxes occurred more rapidly compared to those caused by plant defensins. Furthermore, α-hordothionin caused permeabilization of Fungal hyphae to the non-metabolite α-aminoisobutyric acid and, in addition, altered the electrical properties of artificial lipid bilayers, consistently leading to rupture of the lipid bilayers. The plant defensins did not form ion-permeable pores in artificial Membranes and did not exhibit substantial hyphal Membrane permeabilization activity. Our results are consistent with the notion that thionins inhibit Fungal growth as a result of direct protein-Membrane interactions, whereas plant defensins might act via a different, possibly receptor-mediated, mechanism.
Bruno P. A. Cammue - One of the best experts on this subject based on the ideXlab platform.
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neutral glycolipids of the filamentous fungus neurospora crassa altered expression in plant defensin resistant mutants
Journal of Lipid Research, 2005Co-Authors: Chaeho Park, Bruno P. A. Cammue, Karin Thevissen, Beau Bennion, Isabelle E J A Francois, Kathelijne K A Ferket, Steven B LeveryAbstract:To defend themselves against Fungal pathogens, plants produce numerous antiFungal proteins and peptides, including defensins, some of which have been proposed to interact with Fungal cell surface glycosphingolipid components. Although not known as a phytopathogen, the filamentous fungus Neurospora crassa possesses numerous genes similar to those required for plant pathogenesis identified in Fungal pathogens (Galagan, J. E., et al. 2003. Nature 422: 859-868), and it has been used as a model for studying plant-phytopathogen interactions targeting Fungal Membrane components (Thevissen, K., et al. 2003. Peptides. 24: 1705-1712). For this study, neutral glycolipid components were extracted from wild-type and plant defensin-resistant mutant strains of N. crassa. The structures of purified components were elucidated by NMR spectroscopy and mass spectrometry. Neutral glycosphingolipids of both wild-type and mutant strains were characterized as beta-glucopyranosylceramides, but those of the mutants were found with structurally altered ceramides. Although the wild type expressed a preponderance of N-2'-hydroxy-(E)-Delta3-octadecenoate as the fatty-N-acyl component attached to the long-chain base (4E,8E)-9-methyl-4,8-sphingadienine, the mutant ceramides were found with mainly N-2'-hydroxyhexadecanoate instead. In addition, the mutant strains expressed highly increased levels of a sterol glucoside identified as ergosterol-beta-glucoside. The potential implications of these findings with respect to defensin resistance in the N. crassa mutants are discussed.
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Interactions of antiFungal plant defensins with Fungal Membrane components.
Peptides, 2003Co-Authors: Karin Thevissen, Isabelle E J A Francois, Kathelijne K A Ferket, Bruno P. A. CammueAbstract:Plant defensins are small, basic, cysteine-rich peptides that are generally active against a broad spectrum of Fungal and yeast species at micromolar concentrations. Some of these defensins interact with Fungal-specific lipid components in the plasmaMembrane. Structural differences of these Membrane components between Fungal and plant cells probably account for the selective activity of plant defensins against Fungal pathogens and their nonphytotoxic properties. This review will focus on different classes of complex lipids in Fungal Membranes and on the selective interaction of plant defensins with these complex lipids.
Yasin Aytepe - One of the best experts on this subject based on the ideXlab platform.
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integrated process of Fungal Membrane bioreactor and photocatalytic Membrane reactor for the treatment of industrial textile wastewater
Biochemical Engineering Journal, 2016Co-Authors: Ece Ummu Deveci, Nadir Dizge, Cengiz H Yatmaz, Yasin AytepeAbstract:Abstract In this study, Fungal Membrane bioreactor (FMBR) and semiconductor photocatalytic Membrane reactor (PMR) were used in order to test the efficiency of integrated Fungal biodegradation and photocatalytic degradation of textile wastewater from reactive washing processes. It was found that color removal and chemical oxygen demand (COD) reduction efficiencies were 88% and 53% for photocatalytic degradation, respectively. TiO2 and ZnO were tested as semiconductor catalysts in the PMR and TiO2 showed better efficiencies than ZnO for both color and COD removal. However, it was attained that color removal and COD reduction efficiencies were about 56% and 60% for Fungal biodegradation using Phanerochaete chrysosporium, respectively. Moreover, integrated system in which photocatalytic degradation was employed as a post-treatment application after Fungal biodegradation process achieved high removal efficiencies for color and COD removal as 93% and 99%, respectively.
