The Experts below are selected from a list of 8928 Experts worldwide ranked by ideXlab platform
J.christopher Young - One of the best experts on this subject based on the ideXlab platform.
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microwave assisted extraction of the Fungal Metabolite ergosterol and total fatty acids
Journal of Agricultural and Food Chemistry, 1995Co-Authors: J.christopher YoungAbstract:A novel microwave-assisted extraction (MAE) technique for isolating the Fungal Metabolite ergosterol was developed and applied to Fungal hyphae and spores, mushrooms, filtered air, artificially contaminated corn, naturally contaminated grain dust, and soil. The procedure involves irradiation of milligram-sized samples in a conventional microwave oven for 35 s in the presence of methanol and aqueous sodium hydroxide and results in simultaneous extraction and saponification. Total ergosterol was determined by reversed phase high-performance liquid chromatography with ultraviolet detection at 282 nm and confirmed by gas chromatography-mass spectrometry (GC-MS). Results from a variety of Fungal samples showed MAE-derived ergosterol values to be comparable with those obtained by classical solvent extraction and to significantly exceed those obtained by supercritical fluid extraction (SFE). Total fatty acid profiles of spores were determined (by GC-MS) on the same extracts as used for ergosterol analyses. The MAE procedure is simple, rapid, reliable, and economical with respect to amounts of reagents required, especially when compared with classical solvent and SFE.
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Supercritical fluid extraction and supercritical fluid chromatography of the Fungal Metabolite ergosterol
Journal of Agricultural and Food Chemistry, 1993Co-Authors: J.christopher Young, David E. GamesAbstract:Novel techniques for the CO 2 supercritical fluid extraction and supercritical fluid chromatography of the Fungal Metabolite ergosterol in its free (nonconjugated) form were developed and applied to samples of flour, moldy bread, and mushrooms. Ergosterol was extracted directly, and subsequent chromatography with ultraviolet detection at 282 nm was accomplished without any further sample cleanup. The procedures are simple, rapid, and reliable. Sensitivity on a 1.0-g sample of flour was about 0.05 μg/g. Observed levels of ergosterol ranged from 0.08 μg/g (fresh weight basis) in a cake flour to 14.3 mg/g (freeze-dried basis) in mushroom caps
David E. Games - One of the best experts on this subject based on the ideXlab platform.
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Supercritical fluid extraction and supercritical fluid chromatography of the Fungal Metabolite ergosterol
Journal of Agricultural and Food Chemistry, 1993Co-Authors: J.christopher Young, David E. GamesAbstract:Novel techniques for the CO 2 supercritical fluid extraction and supercritical fluid chromatography of the Fungal Metabolite ergosterol in its free (nonconjugated) form were developed and applied to samples of flour, moldy bread, and mushrooms. Ergosterol was extracted directly, and subsequent chromatography with ultraviolet detection at 282 nm was accomplished without any further sample cleanup. The procedures are simple, rapid, and reliable. Sensitivity on a 1.0-g sample of flour was about 0.05 μg/g. Observed levels of ergosterol ranged from 0.08 μg/g (fresh weight basis) in a cake flour to 14.3 mg/g (freeze-dried basis) in mushroom caps
Patrick F Dowd - One of the best experts on this subject based on the ideXlab platform.
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penifulvin a a sesquiterpenoid derived Metabolite containing a novel dioxa 5 5 5 6 fenestrane ring system from a fungicolous isolate of penicillium griseofulvum
Organic Letters, 2006Co-Authors: Sang Hee Shim, James B Gloer, Dale C Swenson, Patrick F Dowd, Donald T WicklowAbstract:Penifulvin A (1), a new Fungal Metabolite with a previously undescribed ring system, has been isolated from cultures of an isolate of Penicillium griseofulvum (NRRL 35584) obtained from a white mycelial growth on a dead hardwood branch collected in a Hawaiian forest. The structure was assigned by analysis of NMR data and confirmed by single-crystal X-ray diffraction analysis. Penifulvin A (1) shows significant activity in dietary assays against the fall armyworm Spodoptera frugiperda.
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chaetochalasin a a new bioactive Metabolite from chaetomium brasiliense
Tetrahedron Letters, 1998Co-Authors: Dale C Swenson, James B Gloer, Donald T Wicklow, Patrick F DowdAbstract:Abstract Chaetochalasin A ( 1 ), a new bioactive Fungal Metabolite with a previously undescribed ring system, has been isolated from an EtOAc extract of Chaetomium brasiliense (NRRL 22999). The structure of 1 was determined by NMR experiments and single-crystal X-ray diffraction analysis.
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Toxicity of the Fungal Metabolite griseofulvin toHelicoverpa zea andSpodoptera frugiperda
Entomologia Experimentalis et Applicata, 1993Co-Authors: Patrick F DowdAbstract:Griseofulvin is a Fungal Metabolite that can be considered a mycotoxin but is used pharmaceutically to treat topical Fungal infections. It was found to be toxic to both the corn earworm. Helicoverpa zea Boddie, and the fall armyworm, Spodoptera frugiperda (J. E. Smith), at 250 ppm in diets. Its toxicity was enhanced by exposure to ultraviolet light, suggesting it can be photoactivated. Its toxicity was also enhanced by coincorporated δ-amino levulinic acid, suggesting it interferes with porphyrin synthesis in these insects. Differences in toxicity and rates of metabolism caused by coincorporated piperonyl butoxide suggested unspecific monooxygenases were relatively more important in griseofulvin activation in H. zea and detoxification in S. frugiperda .
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Toxicity of the Fungal Metabolite griseofulvin to Helicoverpa zea and Spodoptera frugiperda
Entomologia Experimentalis Et Applicata, 1993Co-Authors: Patrick F DowdAbstract:Griseofulvin is a Fungal Metabolite that can be considered a mycotoxin but is used pharmaceutically to treat topical Fungal infections. It was found to be toxic to both the corn earworm. Helicoverpa zea Boddie, and the fall armyworm, Spodoptera frugiperda (J. E. Smith), at 250 ppm in diets. Its toxicity was enhanced by exposure to ultraviolet light, suggesting it can be photoactivated. Its toxicity was also enhanced by coincorporated δ-amino levulinic acid, suggesting it interferes with porphyrin synthesis in these insects. Differences in toxicity and rates of metabolism caused by coincorporated piperonyl butoxide suggested unspecific monooxygenases were relatively more important in griseofulvin activation in H. zea and detoxification in S. frugiperda.
Margaret A Brimble - One of the best experts on this subject based on the ideXlab platform.
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bioinspired total synthesis and stereochemical revision of the Fungal Metabolite pestalospirane b
Organic Letters, 2017Co-Authors: Sandhya Badrinarayanan, Christopher J Squire, Jonathan Sperry, Margaret A BrimbleAbstract:The total synthesis of both enantiomers of pestalospirane B, 2, has been achieved using a bioinspired tandem dimerization–spiroketalization reaction. Electronic circular dichroism (ECD) and X-ray analysis were used to revise the absolute stereochemistry of the natural product pestalospirane B from 3S, 3′S, 12R, 12′R to its enantiomer 3R, 3′R, 12S, 12′S.
Yufen Zhao - One of the best experts on this subject based on the ideXlab platform.
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mycoediketopiperazine a novel Fungal Metabolite from a papularia sp
Tetrahedron Letters, 2005Co-Authors: Meijuan Fang, Hua Fang, Yaojian Huang, Yufen ZhaoAbstract:A novel Metabolite, mycoediketopiperazine, was isolated from a fungi culture (M-204036). Its structure and absolute stereochemistry was established by spectroscopic studies, including single-crystal X-ray diffraction analysis.
