The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Damià Barceló - One of the best experts on this subject based on the ideXlab platform.
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prospects on coupling uv h2o2 with activated sludge or a Fungal Treatment for the removal of pharmaceutically active compounds in real hospital wastewater
Science of The Total Environment, 2021Co-Authors: Josep Anton Mirtutusaus, Sara Rodriguezmozaz, Damià Barceló, Gloria Caminal, Adrian Jaengil, Gianluigi Buttiglieri, Rafael Gonzalezolmos, Montserrat SarraAbstract:Abstract Conventional active sludge (AS) process at municipal centralized wastewater Treatment facilities may exhibit little pharmaceuticals (PhACs) removal efficiencies when treating hospital wastewater (HWW). Therefore, a dedicated efficient wastewater Treatment at the source point is recommended. In this sense, advanced oxidation processes (AOPs) and Fungal Treatment (FG) have evidenced promising results in degrading PhACs. The coupling of the AOP based on UV/H2O2 Treatment with biological Treatment (AS or FG) treating a real non-sterile HWW, was evaluated in this work. In addition, a coagulation-flocculation preTreatment was applied to improve the efficiency of all approaches. Twenty-two PhACs were detected in raw HWW, which were effectively removed (93–95%) with the combination of any of the biological Treatment followed by UV/H2O2 Treatment. Similar removal results (94%) were obtained when placing UV/H2O2 Treatment before FG, while a lower removal (83%) was obtained in the combination of UV/H2O2 followed by AS. However, the latest was the only Treatment combination that achieved a decrease in the toxicity of water. Moreover, deconjugation of conjugated PhACs has been suggested for ofloxacin and lorazepam after AS Treatment, and for ketoprofen after Fungal Treatment. Monitoring of carbamazepine and its transformation products along the Treatment allowed to identify the same carbamazepine degradation pathway in UV/H2O2 and AS Treatments, unlike Fungal Treatment, which followed another degradation route.
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Fungal Treatment of metoprolol and its recalcitrant metabolite metoprolol acid in hospital wastewater biotransformation sorption and ecotoxicological impact
Water Research, 2019Co-Authors: Adrian Jaengil, Francesc Castelletrovira, Montserrat Sarra, Sara Rodriguezmozaz, Marta Villagrasa, Damià Barceló, Marta LlorcaAbstract:Hospital wastewater (HWW) effluents represent an important source of contaminants such as pharmaceutical compounds and their human metabolites. To better evaluate dedicated Treatment of hospital effluents for pollutant mitigation, not only the parent compounds should be considered but also the intermediates generated during Treatment. The metabolite metoprolol acid (MTPA) has been found in urban wastewaters at higher concentration than its parent compound metoprolol (MTP), being more recalcitrant to biodegradation. The aim of this study was to investigate degradation, transformation and sorption of the β-blocker MTP, and its recalcitrant metabolite MTPA, during water Treatment based on the fungi Ganoderma lucidum, Trametes versicolor and Pleurotus ostreatus. Fourteen intermediates were identified in MTP biotransformation while five of them also attributed to MTPA biodegradation and two to MTPA only. Their identification allowed their correlation in separate biotransformation pathways suggested. The highest degradation rate of metoprolol (up to 51%) and metoprolol acid (almost 77%) was found after 15-days Treatment with Ganoderma lucidum, with an increase in toxicity up to 29% and 4%, respectively. This fungus was further selected for treating real HWW in a batch fluidized bed bioreactor (FBB). Treated wastewater and Fungal biomass samples were used to evaluate the distribution of the target compounds and the intermediates identified between solid and liquid phases. While similar elimination capabilities were observed for the removal of metoprolol, and even higher for its persistent metabolite metoprolol acid, the extent on compound transformation diminished considerably compared with the study treating purified water: a high level of the persistent α-HMTP and TP240 were still present in effluent samples (15% and 6%, respectively), being both TPs present at high proportion (up to 28%) in Fungal biomass. This is the first time that pharmaceutical TPs have been investigated in the Fungal biomass.
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long term continuous Treatment of non sterile real hospital wastewater by trametes versicolor
Journal of Biological Engineering, 2019Co-Authors: Josep Anton Mirtutusaus, Montserrat Sarra, Sara Rodriguezmozaz, Marta Villagrasa, Damià Barceló, Eloi Parlade, Maira Martinezalonso, Nuria Gaju, Gloria CaminalAbstract:Hospital wastewater is commonly polluted with high loads of pharmaceutically active compounds, which pass through wastewater Treatment plants (WWTPs) and end up in water bodies, posing ecological and health risks. White-rot Fungal Treatments can cope with the elimination of a wide variety of micropollutants while remaining ecologically and economically attractive. Unfortunately, bacterial contamination has impeded so far a successful implementation of Fungal Treatment for real applications. This work embodied a 91-day long-term robust continuous Fungal operation treating real non-sterile hospital wastewater in an air pulsed fluidized bed bioreactor retaining the biomass. The hydraulic retention time was 3 days and the ageing of the biomass was avoided through partial periodic biomass renovation resulting in a cellular retention time of 21 days. Evolution of microbial community and Trametes abundance were evaluated. The operation was able to maintain an average pharmaceutical load removal of over 70% while keeping the white-rot fungus active and predominant through the operation.
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stropharia rugosoannulata and gymnopilus luteofolius promising Fungal species for pharmaceutical biodegradation in contaminated water
Journal of Environmental Management, 2018Co-Authors: Francesc Castelletrovira, Sara Rodriguezmozaz, Marta Villagrasa, Daniel Lucas, Damià Barceló, Montserrat SarraAbstract:Abstract Pharmaceuticals are environmental micropollutants that pose an emerging challenge because they are poorly eliminated in conventional wastewater Treatment plants. Over the last decade, many attempts have been made to solve this problem, and wastewater Fungal Treatment is a promising alternative. In this study, six different ligninolytic fungi ( Trametes versicolor , Ganoderma lucidum , Irpex lacteus , Stropharia rugosoannulata , Gymnopilus luteofolius and Agrocybe erebia ) were studied as bioremediation candidates for the removal and degradation of six recalcitrant pharmaceutical micropollutants: Carbamazepine (CBZ), Venlafaxine (VFX), Iopromide (IPD), Diclofenac (DCF), Cyclophosphamide (CFD) and Ifosfamide (IFD). Self-immobilization in a pellet shape was achieved for all Fungal mycelia (which was the first time that this was reported for S. rugosoannulata , G. luteofolius , and A. erebia ). Biodegradation achievement was greater than 90% for IPD with G. luteofolius and greater than 70% for CBZ with S. rugosoannulata , which suggests a great potential for this alternative biological Treatment. Besides, this was the first report where Fungal Treatment achieved CFD and IFD removals greater than 20% for the Treatment with T. versicolor , G. lucidum and S. rugosoannulata .
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the role of sorption processes in the removal of pharmaceuticals by Fungal Treatment of wastewater
Science of The Total Environment, 2018Co-Authors: Daniel Lucas, Francesc Castelletrovira, Montserrat Sarra, Marta Villagrasa, Damià Barceló, Marina Badiafabregat, Teresa Vicent, Gloria Caminal, Sara RodriguezmozazAbstract:The contribution of the sorption processes in the elimination of pharmaceuticals (PhACs) during the Fungal Treatment of wastewater has been evaluated in this work. The sorption of four PhACs (carbamazepine, diclofenac, iopromide and venlafaxine) by 6 different fungi was first evaluated in batch experiments. Concentrations of PhACs in both liquid and solid (biomass) matrices from the Fungal Treatment were measured. Contribution of the sorption to the total removal of pollutants ranged between 3% and 13% in relation to the initial amount. The sorption of 47 PhACs in fungi was also evaluated in a Fungal Treatment performed in 26days in a continuous bioreactor treating wastewater from a veterinary hospital. PhACs levels measured in the Fungal biomass were similar to those detected in conventional wastewater Treatment (WWTP) sludge. This may suggest the necessity of manage Fungal biomass as waste in the same manner that the WWTP sludge is managed.
Sara Rodriguezmozaz - One of the best experts on this subject based on the ideXlab platform.
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prospects on coupling uv h2o2 with activated sludge or a Fungal Treatment for the removal of pharmaceutically active compounds in real hospital wastewater
Science of The Total Environment, 2021Co-Authors: Josep Anton Mirtutusaus, Sara Rodriguezmozaz, Damià Barceló, Gloria Caminal, Adrian Jaengil, Gianluigi Buttiglieri, Rafael Gonzalezolmos, Montserrat SarraAbstract:Abstract Conventional active sludge (AS) process at municipal centralized wastewater Treatment facilities may exhibit little pharmaceuticals (PhACs) removal efficiencies when treating hospital wastewater (HWW). Therefore, a dedicated efficient wastewater Treatment at the source point is recommended. In this sense, advanced oxidation processes (AOPs) and Fungal Treatment (FG) have evidenced promising results in degrading PhACs. The coupling of the AOP based on UV/H2O2 Treatment with biological Treatment (AS or FG) treating a real non-sterile HWW, was evaluated in this work. In addition, a coagulation-flocculation preTreatment was applied to improve the efficiency of all approaches. Twenty-two PhACs were detected in raw HWW, which were effectively removed (93–95%) with the combination of any of the biological Treatment followed by UV/H2O2 Treatment. Similar removal results (94%) were obtained when placing UV/H2O2 Treatment before FG, while a lower removal (83%) was obtained in the combination of UV/H2O2 followed by AS. However, the latest was the only Treatment combination that achieved a decrease in the toxicity of water. Moreover, deconjugation of conjugated PhACs has been suggested for ofloxacin and lorazepam after AS Treatment, and for ketoprofen after Fungal Treatment. Monitoring of carbamazepine and its transformation products along the Treatment allowed to identify the same carbamazepine degradation pathway in UV/H2O2 and AS Treatments, unlike Fungal Treatment, which followed another degradation route.
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Fungal Treatment of metoprolol and its recalcitrant metabolite metoprolol acid in hospital wastewater biotransformation sorption and ecotoxicological impact
Water Research, 2019Co-Authors: Adrian Jaengil, Francesc Castelletrovira, Montserrat Sarra, Sara Rodriguezmozaz, Marta Villagrasa, Damià Barceló, Marta LlorcaAbstract:Hospital wastewater (HWW) effluents represent an important source of contaminants such as pharmaceutical compounds and their human metabolites. To better evaluate dedicated Treatment of hospital effluents for pollutant mitigation, not only the parent compounds should be considered but also the intermediates generated during Treatment. The metabolite metoprolol acid (MTPA) has been found in urban wastewaters at higher concentration than its parent compound metoprolol (MTP), being more recalcitrant to biodegradation. The aim of this study was to investigate degradation, transformation and sorption of the β-blocker MTP, and its recalcitrant metabolite MTPA, during water Treatment based on the fungi Ganoderma lucidum, Trametes versicolor and Pleurotus ostreatus. Fourteen intermediates were identified in MTP biotransformation while five of them also attributed to MTPA biodegradation and two to MTPA only. Their identification allowed their correlation in separate biotransformation pathways suggested. The highest degradation rate of metoprolol (up to 51%) and metoprolol acid (almost 77%) was found after 15-days Treatment with Ganoderma lucidum, with an increase in toxicity up to 29% and 4%, respectively. This fungus was further selected for treating real HWW in a batch fluidized bed bioreactor (FBB). Treated wastewater and Fungal biomass samples were used to evaluate the distribution of the target compounds and the intermediates identified between solid and liquid phases. While similar elimination capabilities were observed for the removal of metoprolol, and even higher for its persistent metabolite metoprolol acid, the extent on compound transformation diminished considerably compared with the study treating purified water: a high level of the persistent α-HMTP and TP240 were still present in effluent samples (15% and 6%, respectively), being both TPs present at high proportion (up to 28%) in Fungal biomass. This is the first time that pharmaceutical TPs have been investigated in the Fungal biomass.
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long term continuous Treatment of non sterile real hospital wastewater by trametes versicolor
Journal of Biological Engineering, 2019Co-Authors: Josep Anton Mirtutusaus, Montserrat Sarra, Sara Rodriguezmozaz, Marta Villagrasa, Damià Barceló, Eloi Parlade, Maira Martinezalonso, Nuria Gaju, Gloria CaminalAbstract:Hospital wastewater is commonly polluted with high loads of pharmaceutically active compounds, which pass through wastewater Treatment plants (WWTPs) and end up in water bodies, posing ecological and health risks. White-rot Fungal Treatments can cope with the elimination of a wide variety of micropollutants while remaining ecologically and economically attractive. Unfortunately, bacterial contamination has impeded so far a successful implementation of Fungal Treatment for real applications. This work embodied a 91-day long-term robust continuous Fungal operation treating real non-sterile hospital wastewater in an air pulsed fluidized bed bioreactor retaining the biomass. The hydraulic retention time was 3 days and the ageing of the biomass was avoided through partial periodic biomass renovation resulting in a cellular retention time of 21 days. Evolution of microbial community and Trametes abundance were evaluated. The operation was able to maintain an average pharmaceutical load removal of over 70% while keeping the white-rot fungus active and predominant through the operation.
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stropharia rugosoannulata and gymnopilus luteofolius promising Fungal species for pharmaceutical biodegradation in contaminated water
Journal of Environmental Management, 2018Co-Authors: Francesc Castelletrovira, Sara Rodriguezmozaz, Marta Villagrasa, Daniel Lucas, Damià Barceló, Montserrat SarraAbstract:Abstract Pharmaceuticals are environmental micropollutants that pose an emerging challenge because they are poorly eliminated in conventional wastewater Treatment plants. Over the last decade, many attempts have been made to solve this problem, and wastewater Fungal Treatment is a promising alternative. In this study, six different ligninolytic fungi ( Trametes versicolor , Ganoderma lucidum , Irpex lacteus , Stropharia rugosoannulata , Gymnopilus luteofolius and Agrocybe erebia ) were studied as bioremediation candidates for the removal and degradation of six recalcitrant pharmaceutical micropollutants: Carbamazepine (CBZ), Venlafaxine (VFX), Iopromide (IPD), Diclofenac (DCF), Cyclophosphamide (CFD) and Ifosfamide (IFD). Self-immobilization in a pellet shape was achieved for all Fungal mycelia (which was the first time that this was reported for S. rugosoannulata , G. luteofolius , and A. erebia ). Biodegradation achievement was greater than 90% for IPD with G. luteofolius and greater than 70% for CBZ with S. rugosoannulata , which suggests a great potential for this alternative biological Treatment. Besides, this was the first report where Fungal Treatment achieved CFD and IFD removals greater than 20% for the Treatment with T. versicolor , G. lucidum and S. rugosoannulata .
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the role of sorption processes in the removal of pharmaceuticals by Fungal Treatment of wastewater
Science of The Total Environment, 2018Co-Authors: Daniel Lucas, Francesc Castelletrovira, Montserrat Sarra, Marta Villagrasa, Damià Barceló, Marina Badiafabregat, Teresa Vicent, Gloria Caminal, Sara RodriguezmozazAbstract:The contribution of the sorption processes in the elimination of pharmaceuticals (PhACs) during the Fungal Treatment of wastewater has been evaluated in this work. The sorption of four PhACs (carbamazepine, diclofenac, iopromide and venlafaxine) by 6 different fungi was first evaluated in batch experiments. Concentrations of PhACs in both liquid and solid (biomass) matrices from the Fungal Treatment were measured. Contribution of the sorption to the total removal of pollutants ranged between 3% and 13% in relation to the initial amount. The sorption of 47 PhACs in fungi was also evaluated in a Fungal Treatment performed in 26days in a continuous bioreactor treating wastewater from a veterinary hospital. PhACs levels measured in the Fungal biomass were similar to those detected in conventional wastewater Treatment (WWTP) sludge. This may suggest the necessity of manage Fungal biomass as waste in the same manner that the WWTP sludge is managed.
Gloria Caminal - One of the best experts on this subject based on the ideXlab platform.
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prospects on coupling uv h2o2 with activated sludge or a Fungal Treatment for the removal of pharmaceutically active compounds in real hospital wastewater
Science of The Total Environment, 2021Co-Authors: Josep Anton Mirtutusaus, Sara Rodriguezmozaz, Damià Barceló, Gloria Caminal, Adrian Jaengil, Gianluigi Buttiglieri, Rafael Gonzalezolmos, Montserrat SarraAbstract:Abstract Conventional active sludge (AS) process at municipal centralized wastewater Treatment facilities may exhibit little pharmaceuticals (PhACs) removal efficiencies when treating hospital wastewater (HWW). Therefore, a dedicated efficient wastewater Treatment at the source point is recommended. In this sense, advanced oxidation processes (AOPs) and Fungal Treatment (FG) have evidenced promising results in degrading PhACs. The coupling of the AOP based on UV/H2O2 Treatment with biological Treatment (AS or FG) treating a real non-sterile HWW, was evaluated in this work. In addition, a coagulation-flocculation preTreatment was applied to improve the efficiency of all approaches. Twenty-two PhACs were detected in raw HWW, which were effectively removed (93–95%) with the combination of any of the biological Treatment followed by UV/H2O2 Treatment. Similar removal results (94%) were obtained when placing UV/H2O2 Treatment before FG, while a lower removal (83%) was obtained in the combination of UV/H2O2 followed by AS. However, the latest was the only Treatment combination that achieved a decrease in the toxicity of water. Moreover, deconjugation of conjugated PhACs has been suggested for ofloxacin and lorazepam after AS Treatment, and for ketoprofen after Fungal Treatment. Monitoring of carbamazepine and its transformation products along the Treatment allowed to identify the same carbamazepine degradation pathway in UV/H2O2 and AS Treatments, unlike Fungal Treatment, which followed another degradation route.
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long term continuous Treatment of non sterile real hospital wastewater by trametes versicolor
Journal of Biological Engineering, 2019Co-Authors: Josep Anton Mirtutusaus, Montserrat Sarra, Sara Rodriguezmozaz, Marta Villagrasa, Damià Barceló, Eloi Parlade, Maira Martinezalonso, Nuria Gaju, Gloria CaminalAbstract:Hospital wastewater is commonly polluted with high loads of pharmaceutically active compounds, which pass through wastewater Treatment plants (WWTPs) and end up in water bodies, posing ecological and health risks. White-rot Fungal Treatments can cope with the elimination of a wide variety of micropollutants while remaining ecologically and economically attractive. Unfortunately, bacterial contamination has impeded so far a successful implementation of Fungal Treatment for real applications. This work embodied a 91-day long-term robust continuous Fungal operation treating real non-sterile hospital wastewater in an air pulsed fluidized bed bioreactor retaining the biomass. The hydraulic retention time was 3 days and the ageing of the biomass was avoided through partial periodic biomass renovation resulting in a cellular retention time of 21 days. Evolution of microbial community and Trametes abundance were evaluated. The operation was able to maintain an average pharmaceutical load removal of over 70% while keeping the white-rot fungus active and predominant through the operation.
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the role of sorption processes in the removal of pharmaceuticals by Fungal Treatment of wastewater
Science of The Total Environment, 2018Co-Authors: Daniel Lucas, Francesc Castelletrovira, Montserrat Sarra, Marta Villagrasa, Damià Barceló, Marina Badiafabregat, Teresa Vicent, Gloria Caminal, Sara RodriguezmozazAbstract:The contribution of the sorption processes in the elimination of pharmaceuticals (PhACs) during the Fungal Treatment of wastewater has been evaluated in this work. The sorption of four PhACs (carbamazepine, diclofenac, iopromide and venlafaxine) by 6 different fungi was first evaluated in batch experiments. Concentrations of PhACs in both liquid and solid (biomass) matrices from the Fungal Treatment were measured. Contribution of the sorption to the total removal of pollutants ranged between 3% and 13% in relation to the initial amount. The sorption of 47 PhACs in fungi was also evaluated in a Fungal Treatment performed in 26days in a continuous bioreactor treating wastewater from a veterinary hospital. PhACs levels measured in the Fungal biomass were similar to those detected in conventional wastewater Treatment (WWTP) sludge. This may suggest the necessity of manage Fungal biomass as waste in the same manner that the WWTP sludge is managed.
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pharmaceuticals removal and microbial community assessment in a continuous Fungal Treatment of non sterile real hospital wastewater after a coagulation flocculation preTreatment
Water Research, 2017Co-Authors: Josep Anton Mirtutusaus, Sara Rodriguezmozaz, Marta Villagrasa, Damià Barceló, Marta Llorca, Eloi Parlade, Maira Martinezalonso, Nuria Gaju, Gloria CaminalAbstract:Hospital wastewaters are a main source of pharmaceutical active compounds, which are usually highly recalcitrant and can accumulate in surface and groundwater bodies. Fungal Treatments can remove these contaminants prior to discharge, but real wastewater poses a problem to Fungal survival due to bacterial competition. This study successfully treated real non-spiked, non-sterile wastewater in a continuous Fungal fluidized bed bioreactor coupled to a coagulation-flocculation preTreatment for 56 days. A control bioreactor without the fungus was also operated and the results were compared. A denaturing gradient gel electrophoresis (DGGE) and sequencing approach was used to study the microbial community arisen in both reactors and as a result some bacterial degraders are proposed. The Fungal operation successfully removed analgesics and anti-inflammatories, and even the most recalcitrant pharmaceutical families such as antibiotics and psychiatric drugs.
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Fungal Treatment for the removal of antibiotics and antibiotic resistance genes in veterinary hospital wastewater
Chemosphere, 2016Co-Authors: Daniel Lucas, Sara Rodriguezmozaz, Damià Barceló, Marina Badiafabregat, Teresa Vicent, Gloria Caminal, Jose Luis BalcazarAbstract:The emergence and spread of antibiotic resistance represents one of the most important public health concerns and has been linked to the widespread use of antibiotics in veterinary and human medicine. The overall elimination of antibiotics in conventional wastewater Treatment plants is quite low; therefore, residual amounts of these compounds are continuously discharged to receiving surface waters, which may promote the emergence of antibiotic resistance. In this study, the ability of a Fungal Treatment as an alternative wastewater Treatment for the elimination of forty-seven antibiotics belonging to seven different groups (β-lactams, fluoroquinolones, macrolides, metronidazoles, sulfonamides, tetracyclines, and trimethoprim) was evaluated. 77% of antibiotics were removed after the Fungal Treatment, which is higher than removal obtained in conventional Treatment plants. Moreover, the effect of Fungal Treatment on the removal of some antibiotic resistance genes (ARGs) was evaluated. The Fungal Treatment was also efficient in removing ARGs, such as ermB (resistance to macrolides), tetW (resistance to tetracyclines), blaTEM (resistance to β-lactams), sulI (resistance to sulfonamides) and qnrS (reduced susceptibility to fluoroquinolones). However, it was not possible to establish a clear link between concentrations of antibiotics and corresponding ARGs in wastewater, which leads to the conclusion that there are other factors that should be taken into consideration besides the antibiotic concentrations that reach aquatic ecosystems in order to explain the emergence and spread of antibiotic resistance.
Daniel Lucas - One of the best experts on this subject based on the ideXlab platform.
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stropharia rugosoannulata and gymnopilus luteofolius promising Fungal species for pharmaceutical biodegradation in contaminated water
Journal of Environmental Management, 2018Co-Authors: Francesc Castelletrovira, Sara Rodriguezmozaz, Marta Villagrasa, Daniel Lucas, Damià Barceló, Montserrat SarraAbstract:Abstract Pharmaceuticals are environmental micropollutants that pose an emerging challenge because they are poorly eliminated in conventional wastewater Treatment plants. Over the last decade, many attempts have been made to solve this problem, and wastewater Fungal Treatment is a promising alternative. In this study, six different ligninolytic fungi ( Trametes versicolor , Ganoderma lucidum , Irpex lacteus , Stropharia rugosoannulata , Gymnopilus luteofolius and Agrocybe erebia ) were studied as bioremediation candidates for the removal and degradation of six recalcitrant pharmaceutical micropollutants: Carbamazepine (CBZ), Venlafaxine (VFX), Iopromide (IPD), Diclofenac (DCF), Cyclophosphamide (CFD) and Ifosfamide (IFD). Self-immobilization in a pellet shape was achieved for all Fungal mycelia (which was the first time that this was reported for S. rugosoannulata , G. luteofolius , and A. erebia ). Biodegradation achievement was greater than 90% for IPD with G. luteofolius and greater than 70% for CBZ with S. rugosoannulata , which suggests a great potential for this alternative biological Treatment. Besides, this was the first report where Fungal Treatment achieved CFD and IFD removals greater than 20% for the Treatment with T. versicolor , G. lucidum and S. rugosoannulata .
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the role of sorption processes in the removal of pharmaceuticals by Fungal Treatment of wastewater
Science of The Total Environment, 2018Co-Authors: Daniel Lucas, Francesc Castelletrovira, Montserrat Sarra, Marta Villagrasa, Damià Barceló, Marina Badiafabregat, Teresa Vicent, Gloria Caminal, Sara RodriguezmozazAbstract:The contribution of the sorption processes in the elimination of pharmaceuticals (PhACs) during the Fungal Treatment of wastewater has been evaluated in this work. The sorption of four PhACs (carbamazepine, diclofenac, iopromide and venlafaxine) by 6 different fungi was first evaluated in batch experiments. Concentrations of PhACs in both liquid and solid (biomass) matrices from the Fungal Treatment were measured. Contribution of the sorption to the total removal of pollutants ranged between 3% and 13% in relation to the initial amount. The sorption of 47 PhACs in fungi was also evaluated in a Fungal Treatment performed in 26days in a continuous bioreactor treating wastewater from a veterinary hospital. PhACs levels measured in the Fungal biomass were similar to those detected in conventional wastewater Treatment (WWTP) sludge. This may suggest the necessity of manage Fungal biomass as waste in the same manner that the WWTP sludge is managed.
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removal of pharmaceuticals from wastewater by Fungal Treatment and reduction of hazard quotients
Science of The Total Environment, 2016Co-Authors: Daniel Lucas, Damià Barceló, Sara RodriguezmozazAbstract:The elimination of 81 pharmaceuticals (PhACs) by means of a biological Treatment based on the fungus Trametes versicolor was evaluated in this work. PhAC removal studied in different types of wastewaters (urban, reverse osmosis concentrate, hospital, and veterinary hospital wastewaters) were reviewed and compared with conventional activated sludge (CAS) Treatment. In addition, hazard indexes were calculated based on the exposure levels and ecotoxicity for each compound and used for the evaluation of the contaminants removal. PhAC elimination achieved with the Fungal Treatment (mean value 76%) was similar or slightly worse than the elimination achieved in the CAS Treatment (85%). However, the Fungal reactor was superior in removing more hazardous compounds (antibiotics and psychiatric drugs) than the conventional activated sludge in terms of environmental risk reduction (93% and 53% of reduction respectively). Fungal Treatment can thus be considered as a good alternative to conventional Treatment technologies for the elimination of PhACs from wastewaters.
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Fungal Treatment for the removal of antibiotics and antibiotic resistance genes in veterinary hospital wastewater
Chemosphere, 2016Co-Authors: Daniel Lucas, Sara Rodriguezmozaz, Damià Barceló, Marina Badiafabregat, Teresa Vicent, Gloria Caminal, Jose Luis BalcazarAbstract:The emergence and spread of antibiotic resistance represents one of the most important public health concerns and has been linked to the widespread use of antibiotics in veterinary and human medicine. The overall elimination of antibiotics in conventional wastewater Treatment plants is quite low; therefore, residual amounts of these compounds are continuously discharged to receiving surface waters, which may promote the emergence of antibiotic resistance. In this study, the ability of a Fungal Treatment as an alternative wastewater Treatment for the elimination of forty-seven antibiotics belonging to seven different groups (β-lactams, fluoroquinolones, macrolides, metronidazoles, sulfonamides, tetracyclines, and trimethoprim) was evaluated. 77% of antibiotics were removed after the Fungal Treatment, which is higher than removal obtained in conventional Treatment plants. Moreover, the effect of Fungal Treatment on the removal of some antibiotic resistance genes (ARGs) was evaluated. The Fungal Treatment was also efficient in removing ARGs, such as ermB (resistance to macrolides), tetW (resistance to tetracyclines), blaTEM (resistance to β-lactams), sulI (resistance to sulfonamides) and qnrS (reduced susceptibility to fluoroquinolones). However, it was not possible to establish a clear link between concentrations of antibiotics and corresponding ARGs in wastewater, which leads to the conclusion that there are other factors that should be taken into consideration besides the antibiotic concentrations that reach aquatic ecosystems in order to explain the emergence and spread of antibiotic resistance.
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suspect screening of emerging pollutants and their major transformation products in wastewaters treated with fungi by liquid chromatography coupled to a high resolution mass spectrometry
Journal of Chromatography A, 2016Co-Authors: Marta Llorca, Daniel Lucas, Damià Barceló, Laura Ferrandocliment, Marina Badiafabregat, Carles Cruzmorato, Sara RodriguezmozazAbstract:A new approach for the screening of 33 pharmaceuticals and 113 of their known transformation products in wastewaters was developed. The methodology is based on the analysis of samples by liquid chromatography coupled to high resolution mass spectrometry (HRMS) followed by data processing using specific software and manual confirmation. A home-made library was built with the transformation products reported in literature for the target pharmaceuticals after Treatment with various fungi. The method was applied to the search of these contaminants in 67 samples generated along Treatment of wastewaters with white-rot fungus Trametes versicolor. The screening methodology allowed the detection of different transformation products (TPs) generated from degradation of parent compounds after Fungal Treatment. This approach can be a useful tool for the rapid screening and tentative detection of emerging contaminants during water Treatment in both full and batch-scale studies when pure standards are not available.
Montserrat Sarra - One of the best experts on this subject based on the ideXlab platform.
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prospects on coupling uv h2o2 with activated sludge or a Fungal Treatment for the removal of pharmaceutically active compounds in real hospital wastewater
Science of The Total Environment, 2021Co-Authors: Josep Anton Mirtutusaus, Sara Rodriguezmozaz, Damià Barceló, Gloria Caminal, Adrian Jaengil, Gianluigi Buttiglieri, Rafael Gonzalezolmos, Montserrat SarraAbstract:Abstract Conventional active sludge (AS) process at municipal centralized wastewater Treatment facilities may exhibit little pharmaceuticals (PhACs) removal efficiencies when treating hospital wastewater (HWW). Therefore, a dedicated efficient wastewater Treatment at the source point is recommended. In this sense, advanced oxidation processes (AOPs) and Fungal Treatment (FG) have evidenced promising results in degrading PhACs. The coupling of the AOP based on UV/H2O2 Treatment with biological Treatment (AS or FG) treating a real non-sterile HWW, was evaluated in this work. In addition, a coagulation-flocculation preTreatment was applied to improve the efficiency of all approaches. Twenty-two PhACs were detected in raw HWW, which were effectively removed (93–95%) with the combination of any of the biological Treatment followed by UV/H2O2 Treatment. Similar removal results (94%) were obtained when placing UV/H2O2 Treatment before FG, while a lower removal (83%) was obtained in the combination of UV/H2O2 followed by AS. However, the latest was the only Treatment combination that achieved a decrease in the toxicity of water. Moreover, deconjugation of conjugated PhACs has been suggested for ofloxacin and lorazepam after AS Treatment, and for ketoprofen after Fungal Treatment. Monitoring of carbamazepine and its transformation products along the Treatment allowed to identify the same carbamazepine degradation pathway in UV/H2O2 and AS Treatments, unlike Fungal Treatment, which followed another degradation route.
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Fungal Treatment of metoprolol and its recalcitrant metabolite metoprolol acid in hospital wastewater biotransformation sorption and ecotoxicological impact
Water Research, 2019Co-Authors: Adrian Jaengil, Francesc Castelletrovira, Montserrat Sarra, Sara Rodriguezmozaz, Marta Villagrasa, Damià Barceló, Marta LlorcaAbstract:Hospital wastewater (HWW) effluents represent an important source of contaminants such as pharmaceutical compounds and their human metabolites. To better evaluate dedicated Treatment of hospital effluents for pollutant mitigation, not only the parent compounds should be considered but also the intermediates generated during Treatment. The metabolite metoprolol acid (MTPA) has been found in urban wastewaters at higher concentration than its parent compound metoprolol (MTP), being more recalcitrant to biodegradation. The aim of this study was to investigate degradation, transformation and sorption of the β-blocker MTP, and its recalcitrant metabolite MTPA, during water Treatment based on the fungi Ganoderma lucidum, Trametes versicolor and Pleurotus ostreatus. Fourteen intermediates were identified in MTP biotransformation while five of them also attributed to MTPA biodegradation and two to MTPA only. Their identification allowed their correlation in separate biotransformation pathways suggested. The highest degradation rate of metoprolol (up to 51%) and metoprolol acid (almost 77%) was found after 15-days Treatment with Ganoderma lucidum, with an increase in toxicity up to 29% and 4%, respectively. This fungus was further selected for treating real HWW in a batch fluidized bed bioreactor (FBB). Treated wastewater and Fungal biomass samples were used to evaluate the distribution of the target compounds and the intermediates identified between solid and liquid phases. While similar elimination capabilities were observed for the removal of metoprolol, and even higher for its persistent metabolite metoprolol acid, the extent on compound transformation diminished considerably compared with the study treating purified water: a high level of the persistent α-HMTP and TP240 were still present in effluent samples (15% and 6%, respectively), being both TPs present at high proportion (up to 28%) in Fungal biomass. This is the first time that pharmaceutical TPs have been investigated in the Fungal biomass.
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long term continuous Treatment of non sterile real hospital wastewater by trametes versicolor
Journal of Biological Engineering, 2019Co-Authors: Josep Anton Mirtutusaus, Montserrat Sarra, Sara Rodriguezmozaz, Marta Villagrasa, Damià Barceló, Eloi Parlade, Maira Martinezalonso, Nuria Gaju, Gloria CaminalAbstract:Hospital wastewater is commonly polluted with high loads of pharmaceutically active compounds, which pass through wastewater Treatment plants (WWTPs) and end up in water bodies, posing ecological and health risks. White-rot Fungal Treatments can cope with the elimination of a wide variety of micropollutants while remaining ecologically and economically attractive. Unfortunately, bacterial contamination has impeded so far a successful implementation of Fungal Treatment for real applications. This work embodied a 91-day long-term robust continuous Fungal operation treating real non-sterile hospital wastewater in an air pulsed fluidized bed bioreactor retaining the biomass. The hydraulic retention time was 3 days and the ageing of the biomass was avoided through partial periodic biomass renovation resulting in a cellular retention time of 21 days. Evolution of microbial community and Trametes abundance were evaluated. The operation was able to maintain an average pharmaceutical load removal of over 70% while keeping the white-rot fungus active and predominant through the operation.
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stropharia rugosoannulata and gymnopilus luteofolius promising Fungal species for pharmaceutical biodegradation in contaminated water
Journal of Environmental Management, 2018Co-Authors: Francesc Castelletrovira, Sara Rodriguezmozaz, Marta Villagrasa, Daniel Lucas, Damià Barceló, Montserrat SarraAbstract:Abstract Pharmaceuticals are environmental micropollutants that pose an emerging challenge because they are poorly eliminated in conventional wastewater Treatment plants. Over the last decade, many attempts have been made to solve this problem, and wastewater Fungal Treatment is a promising alternative. In this study, six different ligninolytic fungi ( Trametes versicolor , Ganoderma lucidum , Irpex lacteus , Stropharia rugosoannulata , Gymnopilus luteofolius and Agrocybe erebia ) were studied as bioremediation candidates for the removal and degradation of six recalcitrant pharmaceutical micropollutants: Carbamazepine (CBZ), Venlafaxine (VFX), Iopromide (IPD), Diclofenac (DCF), Cyclophosphamide (CFD) and Ifosfamide (IFD). Self-immobilization in a pellet shape was achieved for all Fungal mycelia (which was the first time that this was reported for S. rugosoannulata , G. luteofolius , and A. erebia ). Biodegradation achievement was greater than 90% for IPD with G. luteofolius and greater than 70% for CBZ with S. rugosoannulata , which suggests a great potential for this alternative biological Treatment. Besides, this was the first report where Fungal Treatment achieved CFD and IFD removals greater than 20% for the Treatment with T. versicolor , G. lucidum and S. rugosoannulata .
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the role of sorption processes in the removal of pharmaceuticals by Fungal Treatment of wastewater
Science of The Total Environment, 2018Co-Authors: Daniel Lucas, Francesc Castelletrovira, Montserrat Sarra, Marta Villagrasa, Damià Barceló, Marina Badiafabregat, Teresa Vicent, Gloria Caminal, Sara RodriguezmozazAbstract:The contribution of the sorption processes in the elimination of pharmaceuticals (PhACs) during the Fungal Treatment of wastewater has been evaluated in this work. The sorption of four PhACs (carbamazepine, diclofenac, iopromide and venlafaxine) by 6 different fungi was first evaluated in batch experiments. Concentrations of PhACs in both liquid and solid (biomass) matrices from the Fungal Treatment were measured. Contribution of the sorption to the total removal of pollutants ranged between 3% and 13% in relation to the initial amount. The sorption of 47 PhACs in fungi was also evaluated in a Fungal Treatment performed in 26days in a continuous bioreactor treating wastewater from a veterinary hospital. PhACs levels measured in the Fungal biomass were similar to those detected in conventional wastewater Treatment (WWTP) sludge. This may suggest the necessity of manage Fungal biomass as waste in the same manner that the WWTP sludge is managed.
